FR2876029A1 - Cosmetic or dermatological composition, useful for treatment of reactive, sensitive skin, comprises probiotic Lactobacillus and Bifidobacterium species - Google Patents

Abstract

Use of a combination of (i) Lactobacillus paracasei and L. casei, or their fractions or metabolites, and (ii) Bifidobacterium longum or B. lactis, or their fractions or metabolites, to prepare a dermatological composition for treatment and/or prevention of reactive, sensitive skin, optionally where associated with dry skin, is new. An independent claim is also included for cosmetic and/or dermatological compositions containing (A) and (B). ACTIVITY : Dermatological. A composition containing 10 10> colony-forming units each of L. paracasei ST11 NCC 2461 (CNCM I-2116) and B. lactis NCC 2818 (CNCM I-3446) was administered orally, once a day for 8 weeks, to women with dry, sensitive skin. The mean reduction, relative to placebo controls, in clinical scores for roughness of facial skin was 79% and for dryness of the legs 60%, while the mean increase in hydration factor was 28%. MECHANISM OF ACTION : The combination of (A) and (B) acts on the skin barrier and maintains natural defense mechanisms, to promote cutaneous homeostasis and regulation of the immune system of the skin.

Description

The present invention relates to a composition, in particular a cosmetic composition and / or

  dermatological agent intended more particularly for the prevention and / or treatment of skins qualified as sensitive and / or dry skins.

  In general, sensitive skin is defined by a particular reactivity of the skin. However, as opposed to skins described as allergic, this reactivity is not an immunological process that is to say does not occur only in a skin already sensitized, in response to the presence of a allergen. Its mechanism is said to be nonspecific.

  This cutaneous reactivity generally results in the appearance of signs of discomfort in response to contacting the subject with a triggering element that may have various origins. It can be the application of a cosmetic product on the surface of sensitive skin, food intake, exposure to sudden changes in temperature, air pollution and / or radiation rays. ultraviolet or infrared. There are also associated factors like age and skin type. Sensitive skin is therefore more common among dry or oily skin than normal skin.

  The appearance of these signs of discomfort, which appear in the minutes following the contact of the subject with the triggering element, is one of the essential characteristics of sensitive skin. It is mostly dysesthetic sensations. By dysaesthetic sensations we mean more or less painful sensations felt in a cutaneous zone, such as tingling, tingling, itching or pruritus, burning, heating, discomfort, tightness, etc. These subjective signs most often exist in the absence of visible chemical signs such as redness and flaking. It is now known that these skin irritation and intolerance reactions are notably related to a release of neuropeptides by the nerve endings of the epidermis and the dermis.

  However, there is still no fully satisfactory solution to prevent and / or treat this type of skin described as sensitive and this problem is particularly exacerbated in the case where the sensitive skin is associated with dry skin. Dry skin is primarily characterized by tightness and / or tension and is often associated with decreased cutaneous hydration and impaired barrier function as measured by insensitive water loss.

  WO 02/28402 discloses that probiotic microorganisms may have a beneficial effect in the regulation of cutaneous hypersensitivity reactions such as inflammatory and allergic reactions that are the result of an immunological process as opposed to the reactivity of sensitive skin. It is also reported in "Probiotics in the managment of atopic eczema, Clinical and Experimental Allergy 2000", Volume 30, pages 1604-1610, a study reporting the individual effect of some probiotics on infantile immune mechanisms such as dermatitis atopic.

  Unexpectedly, the inventors have found that a combination of two specific probiotic microorganisms proved to be particularly effective, particularly in adults, for the treatment of sensitive skin especially associated with dry skin. The combination of microorganisms, considered according to the invention, advantageously exhibits a potential activity at the level of the cutaneous barrier and a particular valence in the maintenance of the defense mechanisms thus favoring the maintenance of cutaneous homeostasis and the regulation of the immune system. the skin.

  Consequently, according to a first aspect, the present invention relates to a cosmetic and / or dermatological composition, in particular useful for preventing and / or treating sensitive and / or dry skin, comprising in a physiologically acceptable carrier an effective amount of from least one microorganism belonging to the species Lactobacillus paracasei or caseï, one of their fractions or one of their metabolites in combination with an effective amount of at least one microorganism belonging to the species Bifidobacterium longum, one of its fractions or one of its metabolites.

  According to another of its aspects, the present invention relates to a composition for oral absorption, in particular of the dietary supplement type, comprising in a physiologically acceptable carrier an effective amount of at least one microorganism belonging to the species Lactobacillus paracasei or caseol, one of their fractions or one of their metabolites in combination with an effective amount of at least one microorganism belonging to the species Bifidobacterium longum, one of its fractions or one of its metabolites.

  According to another of its aspects, the present invention relates to a cosmetic treatment method for preventing and / or treating sensitive and / or dry skin, comprising the administration, in particular oral or topical, of an effective amount of at least one microorganism belonging to the species Lactobacillus paracasei or casei; one of their fractions or one of their metabolites in combination with an effective amount of at least one microorganism belonging to the species Bifidobacterium longum, one of its fractions or one of its metabolites.

  The present invention further relates, in another of its aspects, to the use of an effective amount of at least one microorganism belonging to the species Lactobacillus paracasei or casei, one of their fractions or one of their metabolites in combination with an effective amount of at least one microorganism belonging to the species Bifidobacterium longum, one of its fractions or one of its metabolites for the preparation of a composition useful for preventing and / or treating skins sensitive and / or dry.

  According to a first variant, this combination is formulated in the form of a composition for oral absorption. It can especially be a food supplement or a foodstuff.

  According to a second variant, it is in the form of a cosmetic and / or dermatological composition according to the invention.

  Sensitive and / or dry skin As previously mentioned, sensitive skin is different from allergic skin. Its reactivity is not an immunological process and usually results only in dysesthetic sensations.

  For obvious reasons, the absence of visible signs makes it difficult to diagnose sensitive skin. Most often this diagnosis is based on the interrogation of the patient. This symptomatology also has the advantage of making it possible to differentiate sensitive skin, whether or not associated with dry skin, from contact irritation or allergy for which, on the other hand, there are visible inflammatory signs.

  Consequently, the development of "sensitive skin" products required the availability of tools for assessing the sensory response of the skin. The first tools were inspired from their conception of the essential characteristic of sensitive skin to know presence of signs of discomfort induced by a topical application. Thus, the "stinging test" with lactic acid was the first test proposed. It is performed by recording tingling sensation reported by a volunteer after application of a lactic acid solution at 10% on the wings of the nose. Subjects reporting moderate or severe tingling sensations are referred to as "stingers" and are considered to be sensitive skin. Because of this cutaneous sensitivity to the topical application of product, these subjects are then selected to test products known as sensitive skin. More recently, to specifically activate the peripheral nerve endings, involved in the discomfort and called nociceptors, recently identified as being involved in sensitive skin, new tests have been proposed that use precisely other discomfort inducers such as capsaicin.

  This second type of test, described in application EP 1 374 913, is also another tool that is particularly useful for the diagnosis of sensitive skin.

  For the purposes of the present invention, sensitive skin covers irritable skin and intolerant skin.

  An intolerant skin is a skin that reacts with sensations of heating, tugging, tingling and / or redness, to various factors such as the application of cosmetic or dermatological products or soap. In general, these signs are associated with erythema and hyper-seborrhoeic or acneic skin, or even rosacea, with or without darter.

  Irritable skin is a skin that reacts with pruritus, that is to say itching or tingling, to various factors such as the environment, emotions, food, wind, friction, razor , hard water with a high concentration of limestone, temperature variations or wool.

  In general, these two types of skin may be associated with dry skin with or without scale or skin that has erythema.

  As previously stated, skin dryness is often associated with a decrease in skin hydration rate, assessed by corneometry and an alteration of the barrier function, measured by the insensitive loss of water.

  Dry skin is essentially a feeling of tightness and / or tension. It is also rough to the touch and appears covered with scales.

  When the skin is slightly dry, these scales are abundant but not visible to the naked eye. They are less and less numerous but more and more visible to the naked eye when this disorder worsens.

  The origin of this dry skin can be constitutional or acquired.

  In the case of acquired dry skin, the intervention of external parameters such as exposure to chemical agents, difficult climatic conditions, solar rays or even certain therapeutic treatments (retinoids, for example) is crucial. Under these outside influences, the skin can become momentarily and locally dry. This can affect any type of skin, normal and even fat.

  In the case of constitutional dry skin, two categories can be distinguished: pathological skin and non-pathological skin.

  The constitutional dry pathological skins are essentially represented by atopic dermatitis and ichthyoses. They are almost independent of external conditions.

  Atopic dermatitis is described as associated with a deficit in the lipid metabolism of the stratum corneum and in particular ceramides. This pathology is in the form of more or less chronic xerosis over a large area of the body, associated with inflammatory and pruriginous plaques.

  Ichthyoses are pathologies characterized by a genetic deficit affecting the process of keratinization at different stages. They are manifested by a large plaque desquamation.

  The non-pathological constitutional dry skin is dry skin whose severity may depend on external factors already mentioned. Included in this skin category are senile skin (characterized by a general decrease in cutaneous metabolism with age), fragile skin (very sensitive to external factors and often accompanied by erythema and rosacea) and vulvar xerosis (d probable genetic origin and manifesting itself primarily on the face, limbs and back of the hands).

  The compositions and process according to the invention thus prove to be particularly effective for preventing and / or treating sensitive and / or dry skin and more particularly for so-called reactive, irritable and / or intolerant skin, dry skin which has been acquired and / or dry skin constitutional.

  The compositions according to the invention comprise at least one microorganism belonging to the species Lactobacillus paracasei or casei, and, in particular, a microorganism belonging to the species Lactobacillus paracasez.

  More particularly, it is the microorganism Lactobacillus paracasei deposited according to the Budapest Treaty at the Pasteur Institute (28 rue du Docteur Roux 75024 Paris Cédex 15) - under the designation CNCM I-2116, of one of its fractions or d one of its metabolites.

  Regarding the microorganism belonging to the species Bifidobacterium Longum, it may more particularly be selected from Bifidobacterium Longum Bb 536 which is a MORINAGA marketed strain, Bifidobacterium Longum deposited under the designation CNCM I-2170 and mixtures thereof.

  According to a particular embodiment, the compositions according to the invention comprise at least the microorganism Lactobacillus paracasei ST11, one of its fractions or one of its metabolites and at least the microorganism Bifidobacterium Longum Bb 536, one of its fractions or one of its metabolites.

  For the purposes of the invention, the term "metabolite" refers to any substance resulting from the metabolism of the microorganisms considered according to the invention and also having an efficacy for the treatment of sensitive and / or dry skin.

  For the purposes of the invention, the term "fraction" refers more particularly to a fragment of said microorganism having an efficacy for the treatment of dry and / or sensitive skin by analogy with said whole microorganism.

  Each of the corresponding microorganisms and / or metabolites and / or fractions can be formulated in a suitable carrier at a rate of at least 103 cfu / g, in particular at a rate of 105 to 1015 cfu / g and more particularly at a rate of 107 to 1012 cfu / g. cfu / g.

  According to a variant of the invention, they can be combined in a ratio of Lactobacillus paracasei or Casei / Bifzdobacterium longum, varying from 0.5 to 1.5, in particular from 0.7 to 1.2, and more particularly from order of 1.

  Advantageously, the compositions according to the invention contain at least said microorganisms in equivalent amounts and more particularly at 1010 cfu respectively.

  These microorganisms can be formulated in the form of powders, that is to say in a dry form, or in the form of suspensions or solutions.

  If necessary, these microorganisms can be formulated within the compositions according to the invention in an encapsulated form so as to significantly improve their survival time. In such a case, the presence of a capsule may in particular delay or prevent degradation of the microorganism in the gastrointestinal tract.

  The compositions according to the invention may also contain at least one microorganism belonging to a species distinct from the species Lactobacillus paracasei or casei and Bifidobacterium longum, in particular of the probiotic type and / or one of its fractions and / or one of its metabolites.

  For the purposes of the present invention, the term "probiotic microorganism" means a living microorganism which, when consumed in an adequate quantity, has a positive effect on the health of its host. and, in particular, that can improve the intestinal microbial equilibrium.

  These microorganisms that are suitable for the invention may be chosen in particular from ascomycetes such as Saccharomyces, Yarrowia, Kluyveromyces, Torulaspora, Schizosaccharomyces pombe, Debaromyces, Candida, Pichia, Aspergillus and Penicillium, bacteria of the genus Bifrdobacterium, Bacteroides, Fusobacterium, Melissococcus, Propionibacterium. , Enterococcus, Lactococcus, Staphylococcus, Peptostrepococcus, Bacillus, Pediococcus, Micrococcus, Leuconostoc, Weissella, Aerococcus, Oenococcus and Lactobacillus and their mixtures.

  Particularly suitable ascomycetes for the present invention are Yarrowia lipolitica and Kluyveromyces lactis, as well as Saccharomyces cereviseae, Torulaspora, Schizosaccharamyces pombe, Candida and Pichia.

  Specific examples of probiotic microorganisms are Bifidobacterium bifidum, Bifrdobacterium infantis, Lactobacillus acidophilus, Lactobacillus alimentarius, Lactobacillus curvatus, Lactobacillus delbruckii subsp. Lactis, Lactobacillus gasseri, Lactobacillus johnsonii, Lactobacillus reuteri, Lactobacillus rhamnosus (Lactobacillus GG), Lactobacillus sake, Lactococcus lactis, Streptococcus thermophilus, Staphylococcus carnosus, and Staphylococcus xylosus and mixtures thereof.

  More particularly, it is probiotic microorganisms from the group of lactic acid bacteria, such as Lactobacillus and / or Bifidobacterium. Illustrative of these lactic acid bacteria, mention may be made of Lactobacillus johnsonii, Lactobacillus reuteri, Lactobacillus rhamnosus, Bifidobacterium bifidum, Bifidobacterium breve, Bifidobacterium animalis, Bifidobacterium lactis, Bifidobacterium infantis, Bifidobacterium adolescentis and Bifidobacterium pseudocatenulatum, and mixtures thereof.

  The most suitable species are Lactobacillus johnsonii, Bifidobacterium adolescentis, respectively deposited according to the Budapest Treaty with the Pasteur Institute (28 rue du Docteur Roux, Paris F75024 cedex 15) under the following designations CNCM I-1225 and CNCM I-2168 and the genus Bifidobacterium lactis (Bb 12) (ATCC27536) and mixtures thereof. The strain of Bifidobacterium lactis (ATCC27536) can be obtained from Hansen (Hansen AIS Chr., 10-12 Boege Alle, PO Box 407, DK2970 Hoersholm, Denmark).

  These microorganisms and / or their fractions and / or metabolites may be formulated in a suitable carrier in an amount of at least 103 cfu / g, in particular at doses ranging from 105 to 1015 cfu / g, and more particularly from 107 to 100 cfu / g. 1012 cfu / g of support.

  The formulations previously described for the microorganisms belonging to the species Lactobacillus paracasei or case and Bifidobacterium Longum can of course be considered for the aforementioned microorganisms.

  In general, the compositions according to the invention and in particular those intended to be administered orally may comprise, for living microorganisms, from 103 to 1015 cfu / g, in particular from 105 to 1015 cfu / g and more particularly from 107 to 1012 cfu / g of microorganisms per gram of support or equivalent doses calculated for inactive or dead microorganisms or for microorganism fractions or metabolites produced. The compositions for topical application according to the invention generally comprise from 103 to 10 12 cfu / g, in particular from 105 to 1010 cfu / g and more particularly from 10 to 109 cfu / g of microorganisms, in particular probiotics.

  When the composition comprises metabolites, the contents of metabolites in the compositions substantially correspond to the levels that can be produced by 103 to 1015 cfu, in particular 105 to 1015 cfu, and more particularly from 107 to 1012 cfu of living microorganisms per gram of support. .

  In the particular case of the compositions to be administered orally, the concentration of each microorganism and / or fraction and / or corresponding metabolite may be adjusted so as to correspond to doses (expressed in microorganism equivalent) ranging from 5.105 to 1013 cfu and in particular from 108 to 1011 cfu / day.

  The microorganism (s) may be included in the composition according to the invention in a living, semi-active or inactivated, dead form.

  I1 (s) can also be included as fractions of cellular components or as metabolites. The microorganism (s), metabolite (s) or fraction (s) can also be introduced in the form of a freeze-dried powder, a culture supernatant and / or the where appropriate in a concentrated form.

  In the particular case of topical compositions, it may be advantageous to use these microorganisms in inactivated or even dead form.

  According to a variant of the invention, the compositions may also contain one or more divalent mineral cation (s).

  In the context of the present invention, the divalent mineral cations can be used in different forms. The divalent mineral cation may thus be in the form of an inorganic or organic salt, anhydrous or hydrated or a chelated complex.

  These salts can be, for example, carbonates, bicarbonates, sulphates, glycerophosphates, chlorides, nitrates, acetates, hydroxides, oxides, salts of α-hydroxy acids (citrates, tartrates, lactates, malates) or of fruit acids, or salts of amino acids (aspartate, arginate, fumarate) or salts of fatty acids (palmitate, oleate, caseinate, behenate).

  According to a particular embodiment, the divalent mineral cation is chosen from manganese, copper and / or zinc.

  According to another particular embodiment, the divalent mineral cation is an alkaline earth metal. As alkaline earth metal used in the invention, mention may be made of barium, calcium, magnesium, strontium and / or beryllium.

  Advantageously, the divalent inorganic cation and in particular alkaline earth metal is used in the present invention in salt form. In particular, the salt may be chosen from calcium nitrate, strontium nitrate, magnesium gluconate, calcium lactate, strontium gluconate, magnesium lactate, calcium chloride, strontium chloride, magnesium chloride, calcium carbonate, strontium sulfate, magnesium sulfate, calcium glycerophosphate, calcium citrate, magnesium citrate, strontium acetate, magnesium acetate and mixtures thereof.

  According to a particularly advantageous embodiment, at least one divalent mineral cation chosen from the salts of citrate, chloride, gluconate, sulfate, lactate and / or acetate, strontium, calcium and / or magnesium and their mixtures is used.

  The divalent mineral cation may also be used in the form of a chelated complex, in particular with crystallized or ionized proteins.

  The divalent inorganic cation may also be in a specific form stored by a microorganism, for example of yeast type, like selenium yeasts.

  Thus, the cations can be introduced as such into the compositions according to the invention or by means of a compound or mixture of compound (s) known to contain at least one of these cations in a high concentration. For example, as a source of metal salts, an extract of plants or yeasts enriched in cations can be used. Similarly, the calcium may for example be introduced via a milk extract.

  The divalent mineral cation content used in the compositions according to the invention depends of course on the form of the cation in question and can be determined by simple routine experiments. These daily doses can in particular range from 100 g to 5 g, more particularly from 1 mg to 2 g, or even from 10 mg to 1.3 g.

  In compositions for oral administration according to the invention, the divalent mineral cation concentration may be adjusted to correspond to doses ranging from 1 to 3000 mg / day and in particular from 10 to 2000 mg / day.

  The compositions according to the invention are generally administered topically or orally.

  The compositions according to the invention may be in any of the galenical forms normally used according to the route of use.

  The support may be of a different nature depending on the type of composition considered.

  Examples of suitable food or pharmaceutical carriers are milk, yoghurt, cheese, fermented milks, fermented milk products, ice creams, fermented cereal products, milk-based powders, formulas for children and infants, especially pet food, tablets or tablets, suspensions of liquid bacteria, oral supplements in dry form and oral supplements in liquid form.

  In particular, the composition according to the invention may be a food composition for human consumption. This may include, in particular, whole nutritional foods, beverages, mineral waters, soups, dietary supplements and substitute foods, nutritional bars, confectionery, milk-based products or fermented milk, yogurt, milk-based powders, enteral nutrition products, children's and / or infants' compositions, cereal-based products or fermented cereal products, ice cream, chocolate, coffee, "culinary" products such as mayonnaise, tomato puree or salad dressings. The composition according to the invention may also be intended for animals.

  As regards more particularly cosmetic products, they may be aqueous, hydroalcoholic or oily solutions, dispersions of the type of solutions or dispersions of the lotion or serum type, emulsions of liquid or semi-liquid consistency of the milk type. , suspensions or emulsions, of the cream type, aqueous or anhydrous gel, microemulsions, microcapsules, microparticles, or vesicular dispersions of ionic and / or nonionic type.

  For ingestion, many embodiments of oral compositions and in particular dietary supplements are possible. Their formulation is carried out by the usual methods for producing dragees, capsules, gels, emulsions, tablets, capsules or solutions. In particular, the active (s) according to the invention can be incorporated into all other forms of food supplements or fortified foods, for example food bars, or compacted powders or not. The powders can be diluted with water, in soda, dairy products or soy derivatives, or incorporated into food bars.

  The active agents according to the invention can be formulated with the excipients and components customary for such oral compositions or food supplements, namely in particular fatty and / or aqueous components, humectants, thickeners, preservatives, texture, flavor and / or coating agents, antioxidants, preservatives and dyes common in the field of food.

  The formulating agents and excipients for oral composition, and especially for food supplements, are known in this field and are not the subject of a detailed description here.

  Of course, the oral compositions according to the invention may contain several other active ingredients.

  As active agents that may be mentioned are vitamins B3, B5, B6, B8, C, E, or PP, carotenoids, curcuminoids and niacin.

  In particular, it is possible to use an antioxidant complex comprising vitamins C and E, and at least one carotenoid, especially a carotenoid selected from 13-carotene, lycopene, astaxanthin, zeaxanthin and lutein, flavonoids such as catechins, hesperidin, proanthocyanidins and anthocyanins.

  The composition advantageously comprises at least one prebiotic or a mixture of prebiotics. More particularly, these prebiotics can be chosen from oligosaccharides, produced from glucose, galactose, xylose, maltose, sucrose, lactose, starch, xylan, hemicellulose, inulin, gums, of the acacia type, for example, or one of their mixtures. More particularly, the oligosaccharide comprises at least one fructooligosaccharide. More particularly, this prebiotic may comprise a mixture of fructo-oligosaccharide and inulin.

  The cosmetic and / or dermatological compositions, more particularly concerned by a topical application, may especially be in the form of aqueous, hydroalcoholic or oily solutions, dispersions of the type of solutions or dispersions of the lotion or serum type, of emulsions of liquid consistency. or semi-liquid of the milk type, obtained by dispersion of a fatty phase in an aqueous phase (HIE) or vice versa (W / O), or suspensions or emulsions of soft, semi-solid or solid consistency of the cream type, aqueous or anhydrous gel, or microemulsions, microcapsules, microparticles, or vesicular dispersions of ionic and / or nonionic type.

  These compositions are prepared according to the usual methods.

  These compositions may in particular constitute creams for cleaning, protection, treatment or care for the face, for the hands, for the feet, for large anatomical folds or for the body, (for example, day creams, night creams). make-up creams, foundation creams, sunscreen creams), make-up products such as fluid foundations, make-up removing milks, protective or skincare body milks, after-sun milks, lotions, skin care gels or mousses, such as cleaning or disinfecting lotions, sunscreen lotions, artificial tanning lotions, bath compositions, deodorant compositions containing a bactericidal agent, gels or lotions after abrasive creams, or compositions against insect bites.

  The compositions according to the invention may also consist of solid preparations constituting soaps or cleaning bars.

  They may also be used for hair in the form of aqueous, alcoholic or aqueous-alcoholic solutions, or in the form of creams, gels, emulsions, foams or in the form of aerosol compositions also containing a propellant under pressure.

  When the composition of the invention is an emulsion, the proportion of the fatty phase can range from 5 to 80% by weight, and preferably from 5 to 50% by weight relative to the total weight of the composition. The oils, emulsifiers and coemulsifiers used in the composition in emulsion form are chosen from those conventionally used in the cosmetics and / or dermatological field. The emulsifier and the coemulsifier may be present in the composition in a proportion ranging from 0.3 to 30% by weight, and preferably from 0.5 to 20% by weight relative to the total weight of the composition.

  When the composition of the invention is a solution or an oily gel, the fatty phase may represent more than 90% of the total weight of the composition.

  In known manner, the cosmetic and / or dermatological composition of the invention may also contain adjuvants which are customary in the pharmaceutical and / or dermatological cosmetic field, such as hydrophilic or lipophilic gelling agents, hydrophilic or lipophilic active agents, preservatives, antioxidants solvents, perfumes, fillers, filters, bactericides, odor absorbers and coloring matter. The amounts of these various adjuvants are those conventionally used in the field under consideration, and for example from 0.01 to 20% of the total weight of the composition. These adjuvants, depending on their nature, can be introduced into the fatty phase and / or into the aqueous phase.

  Fats which can be used in the invention include mineral oils such as, for example, hydrogenated polyisobutene and liquid petroleum jelly, vegetable oils such as, for example, a liquid fraction of shea butter, sunflower oil and almonds. apricot, animal oils such as perhydrosqualene, synthetic oils including Purcellin oil, isopropyl myristate and ethyl hexyl palmitate, and fluorinated oils such as perfluoropolyethers. It is also possible to use fatty alcohols, fatty acids such as stearic acid and, for example, waxes such as paraffin, carnauba and beeswax. It is also possible to use silicone compounds such as silicone oils and, for example, cyclomethicone and dimethicone, waxes, resins and silicone gums. These compounds can be functionalized or not.

  As emulsifiers that can be used in the invention, mention may be made, for example, of glycerol stearate, polysorbate 60, the cetylstearyl alcohol / cetylstearyl alcohol mixture oxyethylenated with 33 moles of ethylene oxide sold under the name Sinnowax AO by Henkel, the mixture of PEG-6 / PEG-32 / glycol stearate sold under the name of Tefose 63 by Gattefosse, PPG-3 myristyl ether, silicone emulsifiers such as cetyldimethicone copolyol and sorbitan mono or tristearate, PEG stearate -40, oxyethylenated sorbitan monostearate (200E).

  As solvents that can be used in the invention, mention may be made of lower alcohols, in particular ethanol and isopropanol, and propylene glycol.

  As hydrophilic gelling agents, mention may be made of carboxylic polymers such as carbomer, acrylic copolymers such as copolymers of acrylates / alkyl acrylates, polyacrylamides and in particular the polyacrylamide mixture, C13-14-Isoparaffin and Laureth-7 sold under the name Sepigel 305 by the company SEPPIC, polysaccharides such as cellulose derivatives such as hydroxyalkylcelluloses and in particular hydroxypropylcellulose and hydroxyethylcellulose, natural gums such as guar, carob and xanthan and clays.

  As lipophilic gelling agents, mention may be made of modified clays such as bentones, metal salts of fatty acids such as aluminum stearates and hydrophobic silica, or else ethylcellulose and polyethylene.

  As hydrophilic active agents, it is possible to use proteins or protein hydrolysates, amino acids, and especially C2 to C10 polyols such as glycerin, sorbitol, butylene glycol and polyethylene glycol, urea, allantoin, sugars and sugar derivatives, water-soluble vitamins, starch, bacterial or plant extracts such as those of Aloe Vera.

  As lipophilic active agents, it is possible to use retinol (vitamin A) and its derivatives, tocopherol (vitamin E) and its derivatives, ceramides, essential oils.

  In addition, the active agents according to the invention may be combined with active agents intended in particular for the prevention and / or treatment of cutaneous affections.

  In addition, the composition of the invention may advantageously contain a thermal and / or mineral water, in particular chosen from Vittel water, the waters of the Vichy basin and Roche Posay water.

  The cosmetic treatment method of the invention may be implemented in particular by applying the cosmetic and / or dermatological compositions as defined above, according to the usual technique of use of these compositions. For example: applications of creams, gels, serums, lotions, cleansing milks or after-sun compositions on the skin or on dry hair, application of a hair lotion on wet hair, shampoos, or still application of toothpaste on the gums.

  The cosmetic process according to the invention can be carried out by topical administration or by daily oral administration, for example, of the combination according to the invention which can be, for example, formulated in the form of capsules, gels, lotions, dragees, emulsions, tablets, capsules or drinkable ampoules, in an appropriate quantity and number, according to their form, so that the active agents are administered at a rate of 5 × 10 5 to 10 13 cfu per day, in particular 106 to 1011 cfu per day, in microorganisms or in doses equivalents of partially inactivated or dead microorganisms or fractions of microorganisms or metabolites produced.

  In another embodiment, administration is repeated until the divalent mineral cation is administered at doses in the range of 1 to 3000 mg per day, and in particular from 10 to 2000 mg per day.

  The method according to the invention may comprise a single administration. According to another embodiment, the administration is repeated for example 2 to 3 times daily on a day or more and generally over an extended period of at least 4 weeks, or even 4 to 15 weeks, with one or more if necessary periods of interruption.

  In the following description and examples, unless otherwise indicated, percentages are percentages by weight and ranges of values in the form "between ... and ..." include the specified lower and upper bounds. The ingredients are mixed, before they are shaped, in the order and under conditions easily determined by those skilled in the art.

  The following examples are presented by way of illustration and not limitation of the field of the invention.

  Examples of compositions for the oral route Example 1: Stick powder Active ingredient Lactobacillus paracasei STIL 101 ufc Bifidobacterium longum Bb536 1010 ufc Calcium citrate 50 mg Excipient xanthan gum 0.8 mg Sodium benzoate 0.2 mg Maltodextrin qsp 30g It is possible to take a stick a day. EXAMPLE 2 Stick Powder Active Principle Lactobacillus paracase® ST11 1010 ufc Bifidobacterium longum Bb536 1010 cfu Magnesium citrate 50 mg Excipient Xanthan gum 0.8 mg Sodium benzoate 0.2 mg Maltodextrin qsp 30 g One stick per day can be taken.

Example 3: capsule

  Active ingredient mg / capsule Lactobacillus paracasei ST11 108 cfu Bifidobacterium longum Bb536 108 cfu Magnesium gluconate 150 Vitamin C 60 magnesium stearate 0.02 One to three of these capsules per day can be taken. Example 4: dragee-type formulation Active ingredients mg / tablet Magnesium gluconate 50 Lactobacillus paracasei ST11 5,108 cfu Bifidobacterium longum Bb536 5,108 cfu Calcium citrate 200 Excipient of the dragee core Microcrystalline cellulose 70 Encompress 1 M 60 Magnesium stearate 3 Anhydrous colloidal silica 1 Coating agent Shellac 5 Talc 61 Sucrose 250 Polyvidone 6 Titanium dioxide 0.3 Coloring agent 5 This type of sugared almond paste can be taken 1 to 3 times a day.

  EXAMPLE 5 Dragée Formulation Active Ingredients mg / tablet Magnesium Lactate 50 Bifidobacterium longum Bb536 10y cfu Lactobacillus paracasei ST11 1 9 cfu Calcium lactate 200 Dread core excipient Microcrystalline cellulose 70 Encompress1M 60 Magnesium stearate 3 Silica colloidal anhydrous 1 Coating agent Shellac 5 Talc 61 Sucrose 250 Polyvinylidone 6 Titanium dioxide 0.3 Staining agent 5 This type of sugar-coated tablet can be taken 1 to 3 times a day.

  Examples of compositions for the topical route Example 1: Facial lotion for sensitive skin Lactobacillus paracasei powder ST11 5.00 Bifidobacterium longum powder Bb536 5.00 Magnesium gluconate 3.00 Calcium lactate 2.00 Antioxidant 0.05 Isopropanol 40.0 Preservative 0, 30 Water qs 100% Example 2: Milk for the care of the face of dry and sensitive skin Magnesium chloride 3.00 Calcium ascorbate 3.00 Lactobacillus paracasei powder ST11 5.00 Bifidobacterium longum powder Bb536 5.00 Glycerol stearate 1.00 cetylstearyl alcohol / cetylstearyl alcohol oxyethylenated at 30 mol EO (Sinnowax A0 sold by the company HENKEL) 3.00 cetyl alcohol 1.00 Dimethicone (DC 200 Fluid sold by the company Dow Corning) 1, 00 Vaseline oil 6.00 Isopropyl myristate (Estol IMP 1514 sold by UNICHEMA) 3.00 Antioxidant 0.05 Glycerin 20.00 Preservative 0.30 Water qs 100 Example 3: Gel for the facial care of sensitive skin Nitrate stronti um 4.00 Powder of Lactobacillus paracasei ST11 5.00 Powder of Bifidobacterium longum Bb536 5.00 Hydroxypropylcellulose (Klucel H sold by the company HERCULES) 1.00 Vitamin E 2.50 Antioxidant 0.05 Isopropanol 40.00 Preservative 0.30 Water qs 100% Example 4: milk for the care of the face of dry and sensitive skins Magnesium ascorbate 3.00 Blackcurrant seed oil 4.00 Borage oil 4.00 Lactobacillus paracasei powder ST11 5.00 Bifidobacterium longum powder Bb536 5.00 glycerol stearate 1.00 cetylstearyl alcohol / cetylstearyl alcohol oxyethylenated to 3 mole EO (Sinnovax AO sold by HENKEL) 3.00 Cetyl alcohol 1.00 Dimethicone (DC 200 Fluid sold by Dow Corning) 1 , 00 Vaseline oil 6.00 Isopropyl myristate (Estol IPM 1514 sold by the company Unichema) 3.00 Glycerin 20.00 Preservative 0, 30 Water qs 100

Claims (24)

  1. Cosmetic and / or dermatological composition, especially useful for preventing and / or treating sensitive and / or dry skin, comprising in a physiologically acceptable carrier an effective amount of at least one microorganism belonging to the species Lactobacillus paracasei or casei; one of their fractions or one of their metabolites in combination with an effective amount of at least one microorganism belonging to the species Bifrdobacterium longum, one of its fractions or one of its metabolites.   2. A composition for oral absorption comprising in a physiologically acceptable carrier an effective amount of at least one microorganism belonging to the species Lactobacillus paracasei or casei; one of their fractions or one of their metabolites in combination with an effective amount of at least one microorganism belonging to the species Bifrdobacterium longum, one of its fractions or one of its metabolites.   3. Composition according to claim 1 or 2, characterized in that the microorganism belonging to the species Lactobacillus paracasei or caseï is the microorganism Lactobacillus paracasei ST11 (CNCM 1-2116).   4. Composition according to any one of claims 1 to 3, characterized in that the microorganism belonging to the species Bifrdobacterium Longum is selected from Bifidobacterium Longum Bb536, Bifrdobacterium Longum deposited under the designation CNCM I-2170 and mixtures thereof.   5. Composition according to any one of the preceding claims, characterized in that it comprises at least the microorganism Lactobacillus paracasei STIL, one of its fractions or one of its metabolites and the microorganism Bifidobacterium Longum Bb 536, the one of its fractions or one of its metabolites.   6. Composition according to any one of the preceding claims, characterized in that each of said microorganisms and / or one of their fractions and / or one of their metabolites is formulated in said support in an amount equivalent to at least 103 cfu / g, in particular ranging from 105 to 1015 cfu / g, and in particular from 107 to 1012 cfu / g of support.   7. Composition according to any one of the preceding claims, characterized in that the said microorganisms are present in a ratio of Ufc Lactobacillus paracasei or Box Bifidobacterium longum varying from 0.5 to 1.5, in particular from 0.7 to 1, 2 and more particularly of the order of 1.   8. Composition according to any one of claims 1 to 7, characterized in that it further comprises at least one other microorganism including probiotic type, and / or one of its fractions and / or one of its metabolites.   9. Composition according to claim 8, characterized in that said microorganism is selected from ascomycetes such as Saccharomyces, Yarrowia, Kluyveromyces, Torulaspora, Schizosaccharomyces pombe, Debaromyces, Candida, Pichia, Aspergillus and Penicillium, bacteria of the genus Bifidobacterium, Bacteroides, Fusobacterium, Melissococcus, Propionibacterium, Enterococcus, Lactococcus, Staphylococcus, Peptostrepococcus, Bacillus, Pediococcus, Micrococcus, Leuconostoc, Weissella, Aerococcus, Oenococcus and Lactobacillus and mixtures thereof.   10. Composition according to any one of claims 8 or 9, characterized in that the microorganism is selected from Saccharomyces cereviseae, Yarrowia lipolitica, Kluyveromyces lactis, Torulaspora, Schizosaccharomyces pombe, Candida, Pichia, Bifidobacterium bifidum, Bifidobacterium breve, Bifidobacterium animalis , Bifidobacterium lactis, Bifidobacterium infantis, Bifidobacterium adolescentis, Bifidobacterium pseudocatenulatum, Lactobacillus acidophilus, Lactobacillus alimentarius, Lactobacillus curvatus, Lactobacillus delbruckii subsp. Lactis, Lactobacillus gasseri, Lactobacillus johnsonii, Lactobacillus reuteri, Lactobacillus rhamnosus (Lactobacillus GG), Lactobacillus cake, Lactococcus lactis, Streptococcus thermophilus, Staphylococcus carnosus and Staphylococcus xylosus and mixtures thereof.   11. Composition according to any one of claims 8 to 10, characterized in that the microorganism is derived from the group of lactic acid bacteria.   12. Composition according to any one of claims 8 to 11, characterized in that the microorganism is selected from Lactobacillus johnsonii (CNCM I-1225), Bifidobacterium adolescentis (CNCM I-2168), Bifidobacterium lactis (ATCC 27536) and their mixtures.   13. Composition according to any one of the preceding claims, characterized in that it further comprises at least one divalent mineral cation.   14. Composition according to claim 13, characterized in that said divalent mineral cation is an alkaline earth metal.   15. The composition of claim 13 or 14, characterized in that said alkaline earth metal is selected from barium, calcium, magnesium, strontium, beryllium and mixtures thereof.   16. Composition according to claim 15, characterized in that said cation is in the form of a salt selected from carbonates, bicarbonates, sulphates, glycerophosphates, chlorides, nitrates, acetates, hydroxides, oxides , salts of ahydroxy acids such as citrates, tartrates, lactates and malates or of fruit acids, salts of amino acids such as aspartates, arginates, and fumarates or salts of fatty acids such as palmitates, oleates, caseinates and behenates.   17. Composition according to any one of the preceding claims, characterized in that it is a cosmetic composition.   18. Composition according to any one of claims 1 to 16, characterized in that it is a food composition, and in particular for human consumption.   19. Composition according to any one of claims 1 to 16, characterized in that it is a dermatological composition.   20. Cosmetic treatment method for preventing and / or treating sensitive skin associated or not with dry skin, comprising the oral or topical administration of at least an effective amount of at least one microorganism belonging to the species Lactobacillus paracasei or casei, one of their fractions or one of their metabolites in combination with an effective amount of at least one microorganism belonging to the species Bifidobacterium longum, one of its fractions or one of its metabolites.   21. The method of claim 20, characterized in that said microorganisms are as defined in claims 2 to 4.   22. The method of claim 20 or 21, characterized in that said microorganisms and / or fractions and / or metabolites are respectively administered at a rate of 5.105 to 1013 cfu per day and in particular 108 to 1011 cfu per day.   23. Use of an effective amount of at least one microorganism belonging to the species Lactobacillus paracasei or casei; one of their fractions or a metabolite thereof in combination with an effective amount of at least one microorganism belonging to the species Bifidobacterium longum, one of its fractions or one of its metabolites, for the manufacture of a dermatological composition for treating and / or preventing reactive sensitive skin associated or not with a dry skin.   24. Use according to claim 23, characterized in that said microorganisms are as defined in claims 2 to 4.