CN106727263B - Skin Tiny ecosystem improves preparation and preparation method thereof - Google Patents
Skin Tiny ecosystem improves preparation and preparation method thereof Download PDFInfo
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- CN106727263B CN106727263B CN201611027229.XA CN201611027229A CN106727263B CN 106727263 B CN106727263 B CN 106727263B CN 201611027229 A CN201611027229 A CN 201611027229A CN 106727263 B CN106727263 B CN 106727263B
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- 238000002360 preparation method Methods 0.000 title claims abstract description 56
- 239000006041 probiotic Substances 0.000 claims abstract description 85
- 235000018291 probiotics Nutrition 0.000 claims abstract description 85
- 230000000529 probiotic effect Effects 0.000 claims abstract description 26
- 239000006228 supernatant Substances 0.000 claims description 50
- 241000894006 Bacteria Species 0.000 claims description 45
- 239000000843 powder Substances 0.000 claims description 31
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 28
- 150000004676 glycans Chemical class 0.000 claims description 15
- 229920001282 polysaccharide Polymers 0.000 claims description 15
- 239000005017 polysaccharide Substances 0.000 claims description 15
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 14
- 239000008103 glucose Substances 0.000 claims description 14
- 239000004310 lactic acid Substances 0.000 claims description 14
- 235000014655 lactic acid Nutrition 0.000 claims description 14
- 230000001954 sterilising effect Effects 0.000 claims description 14
- 238000002156 mixing Methods 0.000 claims description 13
- HQKMJHAJHXVSDF-UHFFFAOYSA-L magnesium stearate Chemical compound [Mg+2].CCCCCCCCCCCCCCCCCC([O-])=O.CCCCCCCCCCCCCCCCCC([O-])=O HQKMJHAJHXVSDF-UHFFFAOYSA-L 0.000 claims description 12
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims description 11
- 240000001046 Lactobacillus acidophilus Species 0.000 claims description 11
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims description 11
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 claims description 11
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims description 11
- 229940039695 lactobacillus acidophilus Drugs 0.000 claims description 11
- 244000199866 Lactobacillus casei Species 0.000 claims description 10
- 230000008901 benefit Effects 0.000 claims description 8
- 239000001963 growth medium Substances 0.000 claims description 8
- 230000003472 neutralizing effect Effects 0.000 claims description 8
- 235000013336 milk Nutrition 0.000 claims description 7
- 239000008267 milk Substances 0.000 claims description 7
- 210000004080 milk Anatomy 0.000 claims description 7
- 235000019359 magnesium stearate Nutrition 0.000 claims description 6
- 230000006872 improvement Effects 0.000 claims description 5
- 239000002671 adjuvant Substances 0.000 claims description 4
- 229940041514 candida albicans extract Drugs 0.000 claims description 4
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 4
- 239000012138 yeast extract Substances 0.000 claims description 4
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 3
- 241000628997 Flos Species 0.000 claims description 3
- 239000001888 Peptone Substances 0.000 claims description 3
- 108010080698 Peptones Proteins 0.000 claims description 3
- 241000698291 Rugosa Species 0.000 claims description 3
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 3
- 229930006000 Sucrose Natural products 0.000 claims description 3
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims description 3
- 239000006071 cream Substances 0.000 claims description 3
- 239000008367 deionised water Substances 0.000 claims description 3
- 229910021641 deionized water Inorganic materials 0.000 claims description 3
- 235000019319 peptone Nutrition 0.000 claims description 3
- 235000020183 skimmed milk Nutrition 0.000 claims description 3
- 239000005720 sucrose Substances 0.000 claims description 3
- 241000218588 Lactobacillus rhamnosus Species 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims description 2
- 241001608472 Bifidobacterium longum Species 0.000 claims 1
- 229940009291 bifidobacterium longum Drugs 0.000 claims 1
- 239000003826 tablet Substances 0.000 abstract description 24
- 238000000034 method Methods 0.000 abstract description 16
- 239000007938 effervescent tablet Substances 0.000 abstract description 14
- 239000000047 product Substances 0.000 abstract description 14
- 230000003020 moisturizing effect Effects 0.000 abstract description 13
- 239000002253 acid Substances 0.000 abstract description 11
- 230000008569 process Effects 0.000 abstract description 7
- 238000004519 manufacturing process Methods 0.000 abstract description 6
- 238000003860 storage Methods 0.000 abstract description 6
- 238000004140 cleaning Methods 0.000 abstract description 5
- 208000027418 Wounds and injury Diseases 0.000 abstract description 4
- 239000000872 buffer Substances 0.000 abstract description 4
- 230000006378 damage Effects 0.000 abstract description 4
- 208000014674 injury Diseases 0.000 abstract description 4
- 230000036559 skin health Effects 0.000 abstract description 4
- 238000012423 maintenance Methods 0.000 abstract description 3
- 239000012228 culture supernatant Substances 0.000 abstract description 2
- 230000008439 repair process Effects 0.000 abstract description 2
- 230000005808 skin problem Effects 0.000 abstract description 2
- 210000003491 skin Anatomy 0.000 description 100
- 239000000654 additive Substances 0.000 description 29
- 239000000243 solution Substances 0.000 description 24
- 230000000996 additive effect Effects 0.000 description 23
- UIIMBOGNXHQVGW-UHFFFAOYSA-M sodium bicarbonate Substances [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 21
- 230000000694 effects Effects 0.000 description 18
- 238000006386 neutralization reaction Methods 0.000 description 15
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 15
- 239000002585 base Substances 0.000 description 14
- 239000007788 liquid Substances 0.000 description 13
- 230000014509 gene expression Effects 0.000 description 12
- 238000000855 fermentation Methods 0.000 description 10
- 230000004151 fermentation Effects 0.000 description 10
- 239000002537 cosmetic Substances 0.000 description 9
- 238000012360 testing method Methods 0.000 description 9
- 241000901050 Bifidobacterium animalis subsp. lactis Species 0.000 description 8
- 229940009289 bifidobacterium lactis Drugs 0.000 description 8
- 239000012530 fluid Substances 0.000 description 8
- 239000003513 alkali Substances 0.000 description 7
- 230000001580 bacterial effect Effects 0.000 description 7
- 230000003139 buffering effect Effects 0.000 description 7
- 239000004615 ingredient Substances 0.000 description 7
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- 238000001035 drying Methods 0.000 description 6
- 244000005700 microbiome Species 0.000 description 6
- 241000194020 Streptococcus thermophilus Species 0.000 description 5
- 239000007853 buffer solution Substances 0.000 description 5
- 239000002609 medium Substances 0.000 description 5
- 238000005119 centrifugation Methods 0.000 description 4
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- 230000000813 microbial effect Effects 0.000 description 4
- 239000003223 protective agent Substances 0.000 description 4
- 239000007921 spray Substances 0.000 description 4
- 241000186000 Bifidobacterium Species 0.000 description 3
- 241000186018 Bifidobacterium adolescentis Species 0.000 description 3
- 241000222120 Candida <Saccharomycetales> Species 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 241000191940 Staphylococcus Species 0.000 description 3
- CKUAXEQHGKSLHN-UHFFFAOYSA-N [C].[N] Chemical compound [C].[N] CKUAXEQHGKSLHN-UHFFFAOYSA-N 0.000 description 3
- 230000004913 activation Effects 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 3
- 238000009395 breeding Methods 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 3
- 210000002615 epidermis Anatomy 0.000 description 3
- 230000001815 facial effect Effects 0.000 description 3
- MCSXGCZMEPXKIW-UHFFFAOYSA-N 3-hydroxy-4-[(4-methyl-2-nitrophenyl)diazenyl]-N-(3-nitrophenyl)naphthalene-2-carboxamide Chemical compound Cc1ccc(N=Nc2c(O)c(cc3ccccc23)C(=O)Nc2cccc(c2)[N+]([O-])=O)c(c1)[N+]([O-])=O MCSXGCZMEPXKIW-UHFFFAOYSA-N 0.000 description 2
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- 241000186866 Lactobacillus thermophilus Species 0.000 description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- 241000235342 Saccharomycetes Species 0.000 description 2
- 230000003712 anti-aging effect Effects 0.000 description 2
- 230000003796 beauty Effects 0.000 description 2
- 238000004364 calculation method Methods 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 230000002500 effect on skin Effects 0.000 description 2
- 238000004108 freeze drying Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000002207 metabolite Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
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- 230000035479 physiological effects, processes and functions Effects 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 239000003755 preservative agent Substances 0.000 description 2
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- 238000012545 processing Methods 0.000 description 2
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- 238000011160 research Methods 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 238000011218 seed culture Methods 0.000 description 2
- 239000002884 skin cream Substances 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- BJEPYKJPYRNKOW-REOHCLBHSA-N (S)-malic acid Chemical compound OC(=O)[C@@H](O)CC(O)=O BJEPYKJPYRNKOW-REOHCLBHSA-N 0.000 description 1
- HLLSOEKIMZEGFV-UHFFFAOYSA-N 4-(dibutylsulfamoyl)benzoic acid Chemical compound CCCCN(CCCC)S(=O)(=O)C1=CC=C(C(O)=O)C=C1 HLLSOEKIMZEGFV-UHFFFAOYSA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 244000131522 Citrus pyriformis Species 0.000 description 1
- FEWJPZIEWOKRBE-JCYAYHJZSA-N Dextrotartaric acid Chemical compound OC(=O)[C@H](O)[C@@H](O)C(O)=O FEWJPZIEWOKRBE-JCYAYHJZSA-N 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 229920002444 Exopolysaccharide Polymers 0.000 description 1
- 206010016807 Fluid retention Diseases 0.000 description 1
- 244000131316 Panax pseudoginseng Species 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
- 241000191967 Staphylococcus aureus Species 0.000 description 1
- KDYFGRWQOYBRFD-UHFFFAOYSA-N Succinic acid Natural products OC(=O)CCC(O)=O KDYFGRWQOYBRFD-UHFFFAOYSA-N 0.000 description 1
- FEWJPZIEWOKRBE-UHFFFAOYSA-N Tartaric acid Natural products [H+].[H+].[O-]C(=O)C(O)C(O)C([O-])=O FEWJPZIEWOKRBE-UHFFFAOYSA-N 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- BJEPYKJPYRNKOW-UHFFFAOYSA-N alpha-hydroxysuccinic acid Natural products OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 235000015278 beef Nutrition 0.000 description 1
- KDYFGRWQOYBRFD-NUQCWPJISA-N butanedioic acid Chemical compound O[14C](=O)CC[14C](O)=O KDYFGRWQOYBRFD-NUQCWPJISA-N 0.000 description 1
- 229910000019 calcium carbonate Inorganic materials 0.000 description 1
- 230000019522 cellular metabolic process Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 210000004087 cornea Anatomy 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 150000004985 diamines Chemical class 0.000 description 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000004945 emulsification Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000006260 foam Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 description 1
- 239000002085 irritant Substances 0.000 description 1
- 231100000021 irritant Toxicity 0.000 description 1
- 235000015122 lemonade Nutrition 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 239000001630 malic acid Substances 0.000 description 1
- 235000011090 malic acid Nutrition 0.000 description 1
- 229940099596 manganese sulfate Drugs 0.000 description 1
- 239000011702 manganese sulphate Substances 0.000 description 1
- 235000007079 manganese sulphate Nutrition 0.000 description 1
- BZDIAFGKSAYYFC-UHFFFAOYSA-N manganese;hydrate Chemical compound O.[Mn] BZDIAFGKSAYYFC-UHFFFAOYSA-N 0.000 description 1
- 238000012009 microbiological test Methods 0.000 description 1
- 230000000474 nursing effect Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 238000005498 polishing Methods 0.000 description 1
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 1
- 229920000053 polysorbate 80 Polymers 0.000 description 1
- 235000013406 prebiotics Nutrition 0.000 description 1
- 108010009004 proteose-peptone Proteins 0.000 description 1
- 230000002829 reductive effect Effects 0.000 description 1
- 210000001732 sebaceous gland Anatomy 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 210000004927 skin cell Anatomy 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- 238000013112 stability test Methods 0.000 description 1
- 238000007655 standard test method Methods 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 230000002459 sustained effect Effects 0.000 description 1
- 238000013268 sustained release Methods 0.000 description 1
- 239000012730 sustained-release form Substances 0.000 description 1
- 239000011975 tartaric acid Substances 0.000 description 1
- 235000002906 tartaric acid Nutrition 0.000 description 1
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 1
- 238000004448 titration Methods 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/19—Cosmetics or similar toiletry preparations characterised by the composition containing inorganic ingredients
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/36—Carboxylic acids; Salts or anhydrides thereof
- A61K8/365—Hydroxycarboxylic acids; Ketocarboxylic acids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
Abstract
The invention discloses a kind of skin Tiny ecosystems to improve preparation, is dissolved in skin base solution and being constituted by probiotics tablets, which, which improves, contains 1 × 10 in preparation6~1 × 108CFU/g active probiotic；The pH that the skin Tiny ecosystem improves preparation is 5.0~5.6；And preparation method thereof.The present invention uses skin base solution of the probiotics fermention culture supernatant as probiotics effervescent tablet, while NaHCO being added in tabletting3Buffer can maintain weak acid environment, reduce injury of the residual of alkaline face cleaning class product for skin.Simultaneously, Tiny ecosystem of the invention, which improves preparation, can guarantee that in production and product storage process, probiotics is active and quantity stability, finally fundamentally adjust the microecological balance of skin, the Maintenance Of Skin Health, there is moisturizing simultaneously, skin softening, the multiple-effect repair function such as compact provide new thinking to solve skin problem.
Description
Technical field
The present invention relates to technical field of skin care, especially a kind of skin Tiny ecosystem improves preparation, and preparation method thereof.
Background technique
Influence of the microorganism to skin, beauty industry the few concern, but with the progress of life science, medicine, biology
Area research person has found that microorganism has vital effect to human body and life, and the quality of human skin has with microorganism
Inseparable relationship.Skin microbial restores to microecological balance and reconstruction plays a crucial role, simultaneously for biological barrier
It constitutes, the decomposition of sebaceous glands, acid cover has been formed and its important role.Skin microbial group and host keep association between environment
It adjusts, physiological dynamic balance, it is beneficial to host, and this balance is exactly skin microecological balance.Due to skin itself,
The reason of environment, drug, their Tiny ecosystem can often lack of proper care, and can be allowed to extensive from extraneous probiotic supplemented or the prebiotic factor
It is multiple.
Probiotics will play cleaning skin, the growth and breeding for inhibiting skin surface harmful bacteria, secretion cornea and exocellular polysaccharide
Help the skin-care effects such as protecting skin, it is necessary to meet two preconditions, first is that probiotics is active, i.e., in the form of viable bacteria
It adds and is present in product；Second is that active probiotic quantity is enough, i.e., certain viable bacteria content just can guarantee active probiotic
Skin effect.
1, Chinese patent: the cosmetics (CN1183266A) containing microecologic regulator, it is characterised in that the cosmetics
Interior youth's Bick Candida containing weight percent 1%~10% and/or deep red saccarom ycete or its metabolin.By implementing this
Inventive technique scheme can further increase skin care, foster skin, strong skin and the promotion skin cell metabolism effect of cosmetics
Fruit, and a kind of indispensable normal physiological bacterium is provided for skin, reach maintenance skin microecological balance.But there are following
Disadvantage: 1. saccharomycete are not skin resident bacteria, can not be colonized on the skin, so the metabolism of skin can not be participated in, and only
There is continual supply that could work to skin, is further improved.2. youth's Bick Candida for using and dark red
Saccharomycete is dead bacterium, can not further growth breeding.3. gram Candida and deep red saccarom ycete do not reach probiotic oral
Rank, so directly application facial skin, may destroy the physiology circulation of human body.
2, Chinese patent: a kind of cosmetics (CN102755281A) containing microecologic regulator, in the cosmetics
In the Bifidobacterium containing weight ratio 0.5%~1%, the microecologic regulator using Bifidobacterium as cosmetics, production
Tiny ecosystem skin care item out can promote skin metabolism, achieve the effect that skin-protecting face nursing；But it has the disadvantage in that used in 1.
Bifidobacterium be one plant of bifidobacterium adolescentis, physiological metabolism is more single.2. bifidobacterium adolescentis is mainly enteron aisle
Probiotics, it is more difficult on the skin to colonize.3. the bifidobacterium adolescentis used is mainly dead bacterium, can not further growth breeding, ginseng
It is recycled with Human Physiology.
3, Chinese patent: beauty method, shield skin composition, thallus and drying thallus (CN103945829A), described
Invention provide it is a kind of reside the staphylococcus epidermis that microbe to screen obtains from human body surface, and in the same of sampling object
Body carries out the investment and surface growth of obtained bacterium, so as to improve the skin care mode of skin health state；But it is lacked there are following
Point: 1. bacterial strains have specificity, do not arrive the staphylococcus epidermis of strain level, can not determine if it is beneficial bacterium, risk
It is too big.2, staphylococcus epidermis and staphylococcus aureus only lean on selective medium to distinguish and identify, and discrimination is inadequate.3, skin care
Although mode is personalized, operation is lengthy and tedious, and technique production is more difficult.
4, Chinese patent: a kind of activated probiotic skin cream and preparation method thereof (CN103251547A), in the patent
In, the active probiotic bacterium solution containing high viable bacteria amount of emulsification system is easily dispersed in using the preparation of bacterium mud, glycerol and trehalose.Secondly
Determine adding conditional and additive amount of the active probiotic bacterium solution in face cream preparation process, it is ensured that face cream posture stablize with
And containing high viable bacteria amount to have effects that skin care benefit skin；But have the disadvantage in that 1: adding 0.1% preservative Nepal first
Rouge is unfavorable for skin health.2, being of no curative effect property of activated probiotic skin cream data obtained；3, the shelf-life is short.
5, Chinese patent: a kind of microbe effervescent tablet and preparation method thereof (CN103585184B) mentions in the patent in question
The method for having supplied to prepare active microorganism effervescent tablet, it is micro- by effectively being extended in the auxiliary material for protecting microbial activity addition effervescent tablet
The preservation time of bio-effervescent tablets；But acid source used is that lemonade, lactic acid, tartaric acid, succinic acid, malic acid etc. are artificial synthesized
Lactic acid, be not natural fermentation products.And disintegration time is not belonging to be sustained in 2.0~3.0min, increases microorganism and is adding
The risk inactivated during work, use, storage.
There are the following problems for application of the probiotics in skin care item at present: probiotics exists with particle or pulvis, absolutely
Most of is dead bacterium, not can be carried out metabolism；Probiotics can not stop for a long time on the skin；Probiotics does not can be uniformly dispersed
In frost or newborn system；Contain preservative in most cosmetics, increase security risk and is unfavorable for probiotics existence.Cause
And active probiotic skin effect and not fully up to expectations.
Summary of the invention
In view of the deficiencies of the prior art, the present invention proposes a kind of skin Tiny ecosystems to improve preparation, and pollution-free, effect is good.
In order to achieve the above-mentioned object of the invention, the present invention is the following technical schemes are provided: a kind of skin Tiny ecosystem improves preparation, by
Probiotics tablets, which are dissolved in skin base solution, to be constituted, which, which improves, contains 1 × 10 in preparation6~1 × 108CFU/g live probiotic
Bacterium；The pH that the skin Tiny ecosystem improves preparation is 5.0~5.6.
Preferably, which is lactobacillus acidophilus, L. casei casei, Lactobacillus rhamnosus, long pair
The mixing of one of discrimination bacillus and lactobacillus thermophilus or a variety of arbitrary proportions.
Preferably, which is that the active probiotic is centrifuged resulting supernatant after fermented and cultured.
Preferably, lactic acid and exocellular polysaccharide are contained in the skin base solution.
A kind of skin Tiny ecosystem as described above improves the preparation method of preparation, comprising the following steps:
(1), probiotics is inoculated into exocellular polysaccharide selective medium and carries out 37~40 DEG C of stationary cultures 17~for 24 hours,
12000~18000rpm is centrifuged 3~10min, obtains probiotic powder and supernatant；After taking 90~95 DEG C of 3~7min of sterilizing of supernatant
It is cooled to room temperature, obtains skin base solution；
(2), NaHCO needed for neutralizing skin base solution is calculated in the titratable acidity and pH value of determination step (1) skin base solution3
Amount, mixes with step (1) probiotic powder and tablet conven-tional adjuvants, and tabletting obtains probiotics tablets, viable bacteria in each probiotics tablets
Number is at least 1 × 109CFU/g。
Preferably, the tablet conven-tional adjuvants include trehalose, glucose, sucrose, milk powder, pearl powder, Flos Rosae Rugosas pollen
And magnesium stearate.
Preferably, the exocellular polysaccharide selective medium are as follows: skimmed milk powder 5%, glucose 5%, yeast extract
0.35%, peptone 0.35%, deionized water 89.3% adjusts pH6.2~6.4.
Compared with prior art, the invention has the following advantages that using probiotics fermention culture supernatant as probiotics
The acid solution of effervescent tablet, while NaHCO being added in tabletting3Buffer can maintain weak acid environment, reduce alkaline face cleaning class product
Remain the injury for skin.
A certain proportion of mixing probiotics is carried out fermented and cultured, centrifuging and taking supernatant first by the present invention.This supernatant fermentation liquid
Be not only hyperalimentation fluid, containing carbon nitrogen source needed for growth of probiotics, and especially comprising natural fermented metabolite lactic acid and
Exocellular polysaccharide.On the one hand exocellular polysaccharide has moisture-keeping function to human skin, on the other hand have protective agent effect to probiotics,
Probiotic active can be protected.Lactic acid is necessary to human skin, and the ability that skin on the one hand can be helped to improve alkali neutralization subtracts
Injury of the residual of few alkalinity face cleaning class product for skin.Meanwhile lactic acid is natural fermentation products, is not artificial synthesized or adds
Add, be used as effervescent tablet acid source, this is an innovation.
Then according to centrifuged supernatant acidity, calculate to neutralize the NaHCO of every gram of centrifuged supernatant3Amount is established and neutralizes public affairs
Formula and model make entire skin care item system pH reach 5.0~5.6.Then the NaHCO that probiotic powder will be mixed and be calculated3
It is matched, unclassified stores mixes in constant humidity desinfection chamber, carries out tabletting, alkali source needed for obtaining effervescent tablet.In order to continue to mention
For carbon source needed for growth of probiotics, contain glucose in tablet material.Trehalose is additionally added in tablet, trehalose is a kind of guarantor
Agent is protected, on the one hand there is efficient biological protective action to probiotics strain cell, biology can be helped to resist extraneous severe ring
Border, and then probiotic active is improved, on the other hand there are the characteristics such as anti-aging, moisturizing, after-sun recovery to skin.Each tablet
Viable count is at least up to 1 × 109CFU/g.Here probiotics is lactobacillus acidophilus, L. casei casei, newborn bifid
Bacillus and streptococcus thermophilus, and be the mixing of one or more arbitrary proportions.
Tablet is put into skin base solution, effervescent tablet slow release, and a large amount of foams will not be generated rapidly, probiotics is made to exist
The ambient growth of one amphimicrobian helps to ensure that probiotics is active during storage and quantity stability.Gained
Skin Tiny ecosystem improve preparation in contain 1 × 106~1 × 108The active probiotic of CFU/g.
Skin Tiny ecosystem of the invention, which improves preparation, can guarantee that probiotics is active in production and product storage process
With quantity stability, finally fundamentally adjust skin microecological balance, the Maintenance Of Skin Health, overcome Traditional makeup/
Skin care item are palliative and are also easy to produce the defect of side effect, while having moisturizing, skin softening, and the multiple-effect repair function such as compact is
It solves skin problem and provides new thinking.Its preparation is by scientific algorithm reaction system and mixed proportion, to the greatest extent
Maintain the active ingredient of each function ingredients.
Active probiotic is exposed under the action of effervescent tablet sustained release and protective agent it is considered that being easy inactivation in this patent
Aqueous systems in.Effervescent tablet use form: probiotics on the one hand can be effectively mixed, it is uniformly distributed；On the other hand, effervescent tablet
A large amount of CO can be generated2, make environment in anaerobism form, greatly facilitate the growth of probiotics of amphimicrobian in survival, ensure that benefit
The activity and stability of raw bacterium.Skin Tiny ecosystem improves preparation cosmetics different from the past, and skin base solution is that probiotics naturally sends out
What ferment obtained, it is limited not only containing skin cares substances such as lactic acid and exocellular polysaccharides, but also containing carbon nitrogen source needed for growth of probiotics
Probiotics is prevented to be easy decline inactivation, stability and activity with higher in aqueous systems.
So one aspect of the present invention introduces effervescent tablet in terms of Tabules, liquid or certain solid actives benefit can be reduced
Inactivation risk of the raw bacterium in processing, storage, use process, is finally reached inhibition harmful bacteria, balance skin surface p H, and moisturizing is protected
Wet effect.On the other hand, solution probiotics, which is exposed in system existing for a large amount of free waters, is easy to decline inactivation, and viable bacteria is steady
Qualitative low, viable bacteria is not easy the technical problems such as evenly dispersed, and the present invention adds the form of natural fermented culture solution using alkali source tablet, and
And in the range of whole system maintains pH5.0~5.6, so that this preparation is to application on human skin more Safe beneficial.
NaHCO in alkali source3There are certain buffering effect, NaHCO3The neutralizing effect of generation is not complete neutralization H+, but
Buffer system is established in N-process, and such balance is had in final system:
Establishing such equilibrium system benefit is after bacterial strain is added, on the one hand can the production acid to bacterial strain buffer, one
Aspect can also constantly discharge CO as bacterial strain produces acid2, make probiotics in the ambient growth of an amphimicrobian, help to protect
Probiotics is active during storage and quantity stability for card.
Detailed description of the invention
Fig. 1 is NaHCO in the present invention3Titrate the titration figure of supernatant；
Fig. 2 is NaHCO in the present invention3Titrate the neutralization ratio of supernatant and the relational graph of titer.
Specific embodiment
Below with reference to embodiment, the present invention will be described in detail, the description of this part be only it is exemplary and explanatory, no
Reply protection scope of the present invention has any restriction effect.
A kind of skin Tiny ecosystem improvement preparation, including probiotics tablets and skin base solution, probiotics tablets are dissolved in skin base solution；Skin
It is 1 × 10 that Tiny ecosystem, which improves the viable count in preparation,6~1 × 108CFU/g；Skin Tiny ecosystem improve preparation pH be 5.0~
5.6。
The preparation that skin Tiny ecosystem improves preparation is obtained using following methods:
1, the acquisition of culture solution
1) activated spawn
Active probiotic is the one of lactobacillus acidophilus, L. casei casei, bifidobacterium lactis and streptococcus thermophilus
Kind is a variety of.
Lactic acid bacteria freeze drying strain is taken, is activated.Freeze-drying pipe disinfection is cut open, a small amount of culture medium is added, bacterium solution is sucked out, into
The coating of row plate, cultivates 48h under 37 DEG C of anaerobic conditions.It selects and meets lactic acid bacteria form, the good bacterium colony of growth and development carries out
Liquid seed culture medium activation, activation condition cultivates 12 under 37 DEG C of anaerobic conditions~for 24 hours, become first order seed.By level-one kind
Son is inoculated into progress activation culture 17h in seed culture medium by the inoculum concentration of 2%~8% (v/v).
MRS Liquid Culture based formulas is as follows:
Casein peptone 10.0g, beef leach object 10.0g, yeast extract 5.0g, glucose 5.0g, sodium acetate 5.0g, lemon
Lemon acid diamine 2.0g, Tween 80 1.0g, dipotassium hydrogen phosphate 2.0g, epsom salt 0.2g, seven water manganese sulfate 0.05g, calcium carbonate
20.0g, distilled water 1.0L, pH6.8.Add agar 20.0g (15g of import) on the basis of MRS solid medium liquid medium within.
118 DEG C, sterilize 30min.
2) strain fermentation
Cell concentration is reached 1 × 107CFU/g inoculation, ferments.Fermentation medium is exocellular polysaccharide selectivity ESM
(expolysaccharides selection medium): skimmed milk powder 5%, glucose 5%, yeast extract 0.35%,
Peptone 0.35%, deionized water 89.3% adjust pH6.2.In 37 DEG C of stationary culture 17h, 10,000rpm centrifugations take supernatant
It is cooled to room temperature after 90~95 DEG C of sterilizing 5min of liquid, the pH of acquisition, titratable acidity.
The culture solution of this centrifuged supernatant, that is, required.It is not only hyperalimentation fluid, containing carbon nitrogen source needed for growth of probiotics,
It and include natural fermented metabolite: lactic acid and exocellular polysaccharide.On the one hand exocellular polysaccharide has moisture-keeping function to human skin,
On the other hand there is protective agent effect to probiotics, probiotic active can be protected.Necessary to human skin, on the one hand lactic acid is
The ability that skin can be helped to improve alkali neutralization reduces injury of the residual of alkaline face cleaning class product for skin.Meanwhile lactic acid
It is natural fermentation products, is not artificial synthesized or addition, is used as effervescent tablet acid source, this is an innovation.
2, alkali source NaHCO3Calculating
Calculate to neutralize the NaHCO of every gram of centrifuged supernatant according to supernatant titratable acidity3Amount, according still further to maximum additive amount
Different weight percentage add NaHCO3, establish and neutralize formula and model, entire skin care item system is made to reach skin microbial growth
Optimal pH 5.0~5.6.NaHCO needed for calculating3The calculating process of quality
NaHCO needed for complete neutralization 1g or 1mL sample3Quality=titratable acidity/° T × 84 × 10-6G/g sample
Wherein, titratable acidity/° T × 84 × 10-6For mol number, it is equivalent to NaHCO3And H+Molal quantity, also correspond to produce
CO2Molal quantity.
So being inserted in above-mentioned formula according to the acidity of supernatant after sterilizing, complete neutralization is calculated and needs NaHCO3's
Amount.Because of NaHCO3Also there is certain buffering effect, therefore buffer system is considered to be made into formula, as shown in Figure 1.By Fig. 1
As can be seen that resulting buffer system is best with the compatible degree of exponential formula.This is because NaHCO3The neutralizing effect of generation is simultaneously
It is not complete neutralization H+, but buffer system is established in the process, such balance is had in final system:
Such equilibrium system benefit be added bacterial strain after, on the one hand can the production acid to bacterial strain buffer, on the one hand
CO can also be constantly discharged as bacterial strain produces acid2.The CO of generation2Be conducive to the growth of probiotics of amphimicrobian.
Bring exponential formula into, when can calculate pH5.6, corresponding titratable acidity.Due to buffer system, dripping
Determine acidity it is relatively high when, balance carry out in one direction, therefore, choose titratable acidity where region, carry out linear push
It leads, as shown in Figure 2.
After bringing formula into, according to the available corresponding theoretical neutralization ratio of titratable acidity.It is counted according to theoretical neutralization ratio
It calculates, obtaining corresponding pH is 5.6 or so.
According to the foundation of buffering formula, 1g fermented supernatant fluid, neutralization reaches NaHCO required for pH5.63Additive amount formula
Are as follows:
In practice with NaHCO needed for 1g or 1mL sample3Quality=titratable acidity/° T × 84 × 10-6G/g sample × reason
By neutralization ratio
3, probiotics tabletting
Viable count will at least reach 1 × 10 in each tablet9Freeze-dry Powder of Probioctics additive amount is calculated in CFU/mL.This
In probiotics be lactobacillus acidophilus, L. casei casei, bifidobacterium lactis and streptococcus thermophilus, and be a kind of or more
The Mixed Microbes of kind arbitrary proportion.The alkali source NaHCO being calculated3Amount is 30%~50%, and glucose additive amount is 5~10%,
Trehalose, glucose, sucrose, milk powder, pearl powder, Flos Rosae Rugosas pollen and the remaining amount of magnesium stearate polishing.Ingredient drying and sterilizing,
It is mixed in desinfection chamber, crosses the sieve sieve of 40 mesh, dehumidifier tabletting.
The supplement of glucose provides sufficient carbon source for the growth of probiotics.Trehalose is a kind of protective agent, on the one hand right
Probiotics strain cell has efficient biological protective action, biology can be helped to resist extraneous adverse circumstances, and then improve benefit
Raw bacterium activity, on the other hand has the characteristics such as anti-aging, moisturizing, after-sun recovery to skin.
4, skin Tiny ecosystem improves the acquisition of preparation
The tablet finally obtained can achieve the effect of slow release in fermented supernatant fluid, will not generate a large amount of bubbles.
Embodiment 1
1, L. casei casei was activated into MRS culture medium for two generations, the thallus of logarithmic phase will be grown into, in born of the same parents
Exo polysaccharides selectivity ESM, 37 DEG C of progress High Density Cultivation 20h, after by fermentation liquid desk centrifuge 10,000rpm is centrifuged
5min obtains supernatant.
2, it is cooled to room temperature after 90 DEG C of sterilizing 5min of gained supernatant, measures its acidity, measure pH3.72, titratable acidity
The supernatant mixed is divided in the blue lid bottle to have sterilized at two by 159.54 ° of T.
3, according to centrifuged supernatant acidity after sterilizing, the theoretical NaHCO for neutralizing every gram of centrifuged supernatant is calculated3Amount is
1.34×10-2g.According to the foundation of buffering formula, 1kg fermented supernatant fluid, neutralization reaches NaHCO required for pH5.63Additive amount
8g NaHCO3。
It 4, is 2.4 × 10 by viable count11CFU/g bifidobacterium lactis carries out tabletting, and the addition of 0.3g tablet bacterium powder is calculated
Amount is 6%, and glucose additive amount is 30%, NaHCO3Additive amount is 40%, and trehalose additive amount is 14%, and milk powder additive amount is
5%, pearl powder additive amount is 5%.
5, except degerming powder, other ingredient drying and sterilizings, interior 40 mesh screens of crossing of desinfection chamber open dehumidifier after mixing.
Station after dehumidifier carries out mixing and tabletting, fermented liquid supernatant are dispensed to spray bottle.
6, tablet is added in skin base solution, no a large amount of bubbles are released rapidly, the uniform slow release of gas, no bottle explosion feelings
Condition, obtaining skin Tiny ecosystem improves preparation, and number of live bacteria of probiotics is 5.4 × 106CFU/g, pH value 5.1.
Embodiment 2
1, lactobacillus acidophilus, L. casei casei, lactobacillus thermophilus are activated two in MRS culture medium respectively
In generation, will grow into the thallus of logarithmic phase, in exocellular polysaccharide selectivity ESM, 37 DEG C of progress High Density Cultivation 17h, culture solution viable bacteria
Number respectively reaches 1.4 × 109、5.5×109、6×109CFU/g, after by fermentation liquid desk centrifuge 12,000rpm carry out from
Heart 5min, takes supernatant.
2, gained supernatant is mixed according to volume ratio 3: 3: 4, obtains probiotics supernatant.Supernatant is gone out at 95 DEG C
It is cooled to room temperature after bacterium 5min, measures its acidity, measure pH3.63,183.80 ° of T of titratable acidity.
3, according to centrifuged supernatant acidity after sterilizing, the theoretical NaHCO for neutralizing every gram of centrifuged supernatant is calculated3Amount is
1.55×10-2g.According to the foundation of buffering formula, 10kg fermented supernatant fluid, neutralization reaches NaHCO required for pH 5.63Addition
Measure 93g NaHCO3。
4, lactobacillus acidophilus and bifidobacterium lactis are mixed according to 1: 1 ratio, total viable count is 7.9 × 1010CFU/g, meter
It is 18% that calculation, which obtains 0.15g tablet bacterium powder additive amount, and glucose additive amount is 10%, NaHCO3Additive amount is 30%, trehalose
Additive amount is 12%, and milk powder additive amount is 15%, and pearl powder additive amount is 10%, magnesium stearate 5%.
5, except degerming powder, other ingredient drying and sterilizings, interior 40 mesh screens of crossing of desinfection chamber open dehumidifier after mixing.
Station after dehumidifier carries out mixing and tabletting, fermented liquid supernatant are dispensed to spray bottle.
6, tablet is added in skin base solution, no a large amount of bubbles are released rapidly, the uniform slow release of gas, no bottle explosion feelings
Condition, obtaining skin Tiny ecosystem improves preparation, and number of live bacteria of probiotics is 8 × 106CFU/g, pH value 5.5.
Embodiment 3
1, lactobacillus acidophilus, L. casei casei, bifidobacterium lactis are activated two in MRS culture medium respectively
In generation, will grow into the thallus of logarithmic phase, in exocellular polysaccharide selectivity ESM, 37 DEG C of progress High Density Cultivations, culture solution viable count point
Do not reach 5.5 × 109、4×109、9×109CFU/g, after by fermentation liquid desk centrifuge 9,000rpm carries out centrifugation 15min,
Take supernatant.
2, gained supernatant is mixed according to volume ratio 1: 3: 3, arrives probiotics supernatant.Supernatant is sterilized at 95 DEG C
It is cooled to room temperature after 5min, measures its acidity, measure pH3.71,184.53 ° of T of titratable acidity.
3, according to centrifuged supernatant acidity after sterilizing, the theoretical NaHCO for neutralizing every gram of centrifuged supernatant is calculated3Amount is
1.55×10-2g.According to the foundation of buffering formula, 100kg fermented supernatant fluid, neutralization reaches NaHCO required for pH5.63Addition
Measure 0.93kg NaHCO3。
4, lactobacillus acidophilus and bifidobacterium lactis are mixed according to 4: 1 ratios, total viable count is 1.4 × 1011CFU/g, meter
It is 10% that calculation, which obtains 0.35g tablet bacterium powder additive amount, and glucose additive amount is 15%, NaHCO3Additive amount is 40%, trehalose
Additive amount is 15%, and milk powder additive amount is 10%, and pearl powder additive amount is 5%, magnesium stearate 5%.
5, except degerming powder, other ingredient drying and sterilizings, interior 40 mesh screens of crossing of desinfection chamber open dehumidifier after mixing.
Station after dehumidifier carries out mixing and tabletting, fermented liquid supernatant are dispensed to spray bottle.
6, tablet is added in skin base solution, no a large amount of bubbles are released rapidly, the uniform slow release of gas, no bottle explosion feelings
Condition, obtaining skin Tiny ecosystem improves preparation, and number of live bacteria of probiotics is 1 × 107CFU/g, pH value 5.4.
Embodiment 4
1, lactobacillus acidophilus, L. casei casei, bifidobacterium lactis, streptococcus thermophilus are cultivated in MRS respectively
It activated for two generations in base, the thallus of logarithmic phase will be grown into, in exocellular polysaccharide selectivity ESM, 37 DEG C of progress High Density Cultivation 17h,
Culture solution viable count respectively reaches 5.5 × 109、4×109、6×109、4.5×109CFU/g, after by the desk-top centrifugation of fermentation liquid
Machine 12,000rpm carry out centrifugation 5min, take supernatant.
2, gained supernatant is mixed according to volume ratio 1: 1: 1: 1, arrives probiotics supernatant.Supernatant is gone out at 90 DEG C
It is cooled to room temperature after bacterium 5min, measures its acidity, measure pH3.69,185.35 ° of T of titratable acidity.
3, according to centrifuged supernatant acidity after sterilizing, the theoretical NaHCO for neutralizing every gram of centrifuged supernatant is calculated3Amount is
1.55×10-2g.According to the foundation of buffering formula, 100kg fermented supernatant fluid, neutralization reaches NaHCO required for pH5.63Addition
Measure 0.93kg NaHCO3。
4, by lactobacillus acidophilus, L. casei casei, bifidobacterium lactis, streptococcus thermophilus according to 1: 1: 1: 1 ratio
Example mixing, total viable count are 2.9 × 1011CFU/g, it is 10% that 0.5g tablet bacterium powder additive amount, which is calculated, glucose additive amount
For 25%, NaHCO3Additive amount is 35%, and trehalose additive amount is 8%, and milk powder additive amount is 2%, and pearl powder additive amount is
10%, magnesium stearate 10%.
5, except degerming powder, other ingredient drying and sterilizings, interior 40 mesh screens of crossing of desinfection chamber open dehumidifier after mixing.
Station after dehumidifier carries out mixing and tabletting, fermented liquid supernatant are dispensed to spray bottle.
6, tablet is added in skin base solution, no a large amount of bubbles are released rapidly, the uniform slow release of gas, no bottle explosion feelings
Condition, obtaining skin Tiny ecosystem improves preparation, and number of live bacteria of probiotics is 5 × 107CFU/g, pH value 5.2.
Skin Tiny ecosystem made from examples detailed above improves the stability test of preparation and active probiotic piece, by 4 series
Sample probiotics viable bacteria in sample is measured by sampling, often in 0d, 3d, 7d, 15d, 30d in 25 DEG C of preservation 30d under room temperature
A sample takes three Duplicate Samples, and final result is the average value of 3 sample viable counts.According to " GB7918.1-7918.5-1987
Cosmetics microorganism standard test method " processing sample, according to " GBT 4789.35-2010 microbiological test of food hygiene is eaten
Lactic acid bacteria is examined in product " select detection culture medium and condition of culture.Test result is referring to following table:
1 different skin Tiny ecosystem of table improves stability of the preparation at room temperature in preserving process
Seen from table 1, the resulting skin Tiny ecosystem of this patent technique improves preparation viable bacteria content height and viable bacteria stability is good.
Stability of the 2 different activities probiotics tablets of table in preserving process
As can be seen from Table 2, the viable bacteria content of the resulting active probiotic piece of this patent technique is high and viable bacteria stability is good.
Health giving quality test
This research is chosen 400 adult females and is tested on the face, and the age is 25~55 years old, is divided into 4 groups, every group
100 people.This four groups skin Tiny ecosystems used correspondingly containing Examples 1 to 4 improve preparation.Frequency of use is one day 2
It~3 times, persistently uses 4 weeks., the colour of skin uniformity horizontal to moisturizing and irritation carry out test observation respectively, and by volunteer into
The assessment of row questionnaire, it is comprehensive to obtain data.Test result is as follows.
A, moisturizing is horizontal
The test result of moisturizing level is as shown in table 3.In table 3,5 points of expression moisturizing levels are very good, 4 points of expression moisture retention waters
Flat preferable, 3 points of expression moisturizings are horizontal general, and 2 points of expression moisturizing levels are poor, and 1 point of expression moisturizing level is excessively poor.
Table 3
| Product | 5 points | 4 points | 3 points | 2 points | 1 point |
| Embodiment 1 | 85 | 15 | 0 | 0 | 0 |
| Embodiment 2 | 35 | 50 | 15 | 0 | 0 |
| Embodiment 3 | 78 | 10 | 12 | 0 | 0 |
| Embodiment 4 | 32 | 48 | 20 | 0 | 0 |
From the point of view of the test result for the moisturizing level that volunteer's facial skin is tested, skin Tiny ecosystem improves preparation to skin
Moistening effect is obvious.
B, the colour of skin uniformity
The test result of the colour of skin uniformity is as shown in table 4.In table 4,5 points of expression colour of skin uniformitys are obviously improved, 4 points of expressions
The colour of skin uniformity makes moderate progress, and 3 points of expression colour of skin uniformity indistinctions, 2 points of expression colour of skin uniformitys are deteriorated, 1 point of expression
The colour of skin uniformity is deteriorated.
Table 4
| Product | 5 points | 4 points | 3 points | 2 points | 1 point |
| Embodiment 1 | 10 | 53 | 27 | 0 | 0 |
| Embodiment 2 | 8 | 22 | 70 | 0 | 0 |
| Embodiment 3 | 15 | 52 | 33 | 0 | 0 |
| Embodiment 4 | 27 | 45 | 28 | 0 | 0 |
From the point of view of the test result for the colour of skin uniformity that volunteer's facial skin is tested, improve preparation using skin Tiny ecosystem
The blee of volunteer have improvement, it is basic the case where being deteriorated.
C, irritation
Irritating test result is as shown in table 5.In table 5,5 points of expressions are nonirritant, and 4 points indicate minimal irritation, and 3
Dividing indicates medium irritation, 2 points of expression intense irritations, and 1 point indicates strongly irritation.
Table 5
| Product | 5 points | 4 points | 3 points | 2 points | 1 point |
| Embodiment 1 | 100 | 0 | 0 | 0 | 0 |
| Embodiment 2 | 75 | 25 | 0 | 0 | 0 |
| Embodiment 3 | 84 | 16 | 0 | 0 | 0 |
| Embodiment 4 | 91 | 9 | 0 | 0 | 0 |
From the point of view of irritant experiment result, the preparation for being added to active probiotic ingredient does not have or only has to volunteer's skin
Minimal irritation.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (5)
1. the preparation method that a kind of skin Tiny ecosystem improves preparation, comprising the following steps: (1), by probiotics be inoculated into extracellular more
37~40 DEG C of stationary cultures 17~for 24 hours are carried out in sugared selective medium, 12000~18000rpm is centrifuged 3~10min, benefits
Raw bacterium powder and supernatant；It is cooled to room temperature after taking 90~95 DEG C of 3~7min of sterilizing of supernatant, obtains skin base solution；
(2), NaHCO needed for neutralizing skin base solution is calculated in the titratable acidity and pH value of determination step (1) skin base solution3Amount, with
Step (1) probiotic powder and tablet conven-tional adjuvants mix, and tabletting obtains probiotics tablets, and viable count is at least in each probiotics tablets
It is 1 × 109CFU/g；
The skin Tiny ecosystem improvement preparation is dissolved in skin base solution by probiotics tablets to be constituted；
The probiotics is lactobacillus acidophilus, L. casei casei, Lactobacillus rhamnosus, bifidobacterium longum and thermophilic cream
The mixing of one of bacillus or a variety of arbitrary proportions；
The skin base solution is that the probiotics is centrifuged resulting supernatant after fermented and cultured.
2. the preparation method that skin Tiny ecosystem as described in claim 1 improves preparation, it is characterised in that: the skin Tiny ecosystem changes
Contain 1 × 10 in kind preparation6~1 × 108CFU/g active probiotic；The pH that the skin Tiny ecosystem improves preparation is 5.0~5.6.
3. the preparation method that skin Tiny ecosystem as claimed in claim 1 or 2 improves preparation, it is characterised in that: the skin base solution
In contain lactic acid and exocellular polysaccharide.
4. the preparation method that skin Tiny ecosystem as described in claim 1 improves preparation, it is characterised in that: the tablet conven-tional adjuvants
Including trehalose, glucose, sucrose, milk powder, pearl powder, Flos Rosae Rugosas pollen and magnesium stearate.
5. the preparation method that skin Tiny ecosystem as described in claim 1 improves preparation, it is characterised in that: the exocellular polysaccharide choosing
Selecting property culture medium are as follows: skimmed milk powder 5%, glucose 5%, yeast extract 0.35%, peptone 0.35%, deionized water
89.3%, adjust pH 6.2~6.4.
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| CN108192779A (en) * | 2017-12-30 | 2018-06-22 | 高产明 | A kind of skin care scented soap and preparation method thereof |
| CN108066275A (en) * | 2018-01-30 | 2018-05-25 | 集美大学 | A kind of edible rose facial mask and preparation method thereof |
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| CN110025501B (en) * | 2019-05-13 | 2022-01-18 | 武汉森澜生物科技有限公司 | Composition for supplementing moisture to skin and preparation method and application thereof |
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| CN113081945A (en) * | 2021-05-17 | 2021-07-09 | 山东君捌壹生物科技有限公司 | Probiotics extract-containing compound polyhexamethylene biguanide salt no-wash hand sanitizer and preparation method thereof |
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5324515A (en) * | 1991-10-22 | 1994-06-28 | Pacific Corporation | Cosmetic material obtained from a lactic acid fermentation broth |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2058702A (en) * | 2000-10-06 | 2002-04-15 | Nestle Sa | Use of probiotic lactic acid bacteria for balancing the skin's immune system |
| FR2876029B1 (en) * | 2004-10-04 | 2008-11-14 | Oreal | COSMETIC AND / OR DERMATOLOGICAL COMPOSITION FOR SENSITIVE SKINS. |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US5324515A (en) * | 1991-10-22 | 1994-06-28 | Pacific Corporation | Cosmetic material obtained from a lactic acid fermentation broth |
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