CN113081945A - Probiotics extract-containing compound polyhexamethylene biguanide salt no-wash hand sanitizer and preparation method thereof - Google Patents
Probiotics extract-containing compound polyhexamethylene biguanide salt no-wash hand sanitizer and preparation method thereof Download PDFInfo
- Publication number
- CN113081945A CN113081945A CN202110535895.9A CN202110535895A CN113081945A CN 113081945 A CN113081945 A CN 113081945A CN 202110535895 A CN202110535895 A CN 202110535895A CN 113081945 A CN113081945 A CN 113081945A
- Authority
- CN
- China
- Prior art keywords
- probiotic
- hand sanitizer
- extract
- polyhexamethylene biguanide
- fermentation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
- A61K8/345—Alcohols containing more than one hydroxy group
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/84—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds obtained by reactions otherwise than those involving only carbon-carbon unsaturated bonds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9794—Liliopsida [monocotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/005—Antimicrobial preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/10—Washing or bathing preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Dermatology (AREA)
- Biotechnology (AREA)
- Engineering & Computer Science (AREA)
- Emergency Medicine (AREA)
- Botany (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention belongs to the technical field of disinfection, cleaning and sanitation application, and particularly relates to a probiotic extract-containing compounded polyhexamethylene biguanide salt no-clean hand sanitizer and a preparation method thereof. The composition comprises the following effective components in percentage by weight: 10 to 20 percent of probiotic fermentation product; 2 to 5 percent of plant extract; 0.8 to 1 percent of medical glycerol; 0.25 to 0.4 percent of polyhexamethylene biguanide hydrochloride; a pH adjusting agent; the balance of water, wherein the pH regulator is used for regulating the pH value of the hand sanitizer to weak acidity, and the probiotic fermentation product is supernatant obtained after probiotic fermentation and separation. According to the invention, the probiotic fermentation product is creatively compounded with the polyhexamethylene biguanide hydrochloride and the medical glycerol, so that the obtained hand sanitizer not only has long-acting sterilization and bacteriostasis effects, but also has the effects of moisturizing and moistening hand skin, and meanwhile, the supernatant liquid obtained in the process of probiotic fermentation is used as a raw material, so that resource waste is avoided, and the purposes of environmental protection and energy conservation are achieved.
Description
Technical Field
The invention belongs to the technical field of disinfection, cleaning and sanitation application, and particularly relates to a probiotic extract-containing compounded polyhexamethylene biguanide salt no-clean hand sanitizer and a preparation method thereof.
Background
Along with improvement of living standards of people and convenient use of transportation modes, people can frequently go out for travel, gather meals, shop and other public activities, so that hands of people are easy to contact various pathogenic microorganisms such as bacteria, viruses and the like, if the pathogenic microorganisms cannot be timely and effectively removed, the pathogenic microorganisms can survive for hours at palms, finger joints, fingertips and other positions, and even can continue to grow and propagate, and the pathogenic microorganisms and the viruses can be hidden and propagated places. For example, contaminated hands can scratch the nose and rub the eyes without being cleaned, germs on the hands can enter the hands in a false manner, and easily enter the human body through respiratory tracts, nasal and ocular membranes and the like; the hands can take things to eat without disinfection, and microorganisms such as bacteria, viruses and the like on the hands can be transferred to food, so that people can easily infect various diseases after eating the food; before the towel is used, hands are not cleaned, germs on the hands are transferred to the towel, and when a face is washed, the towel contacts with the eye strength membrane, the nose strength membrane and the like, and the germs can enter a human body by riding the machine.
The hand sanitizer is a cleaning solution for cleaning hands, wherein certain specific components can sterilize and disinfect the hand sanitizer, and the hand sanitizer is convenient to use, can be used for cleaning and disinfecting the surface of skin and also can be used for cleaning and disinfecting the surface of an object. Currently, the hand sanitizer is mainly high-concentration alcohols such as ethanol and the like, phenols such as triclosan and the like, quaternary ammonium salts such as benzalkonium chloride, didodecyldimethylammonium chloride and the like, and guanidines such as chlorhexidine acetate, polyhexamethylene biguanide and the like. Although the quaternary ammonium salt cationic hand sanitizer is widely used, the quaternary ammonium salt cationic hand sanitizer serving as organic micromolecules has the problems of easy volatilization, poor chemical stability, slightly high toxicity and irritation and the like. Chlorhexidine acetate has limited use due to its peculiar odor. Polyhexamethylene biguanide as an environment-friendly degradable high-molecular polymer disinfectant has higher safety, mildness and bactericidal property compared with other salts, but has higher cost and is not beneficial to market production and application. In addition, the single chemical synthesis type hand sanitizer is easy to generate drug resistance for microorganisms after being used for a long time in the same system, so that the sterilization effect is reduced. Therefore, the hand sanitizer is compounded, and the synergistic action and action mechanism of the hand sanitizer are researched, so that the sterilization effect of the hand sanitizer can be enhanced, the antibacterial spectrum of the hand sanitizer can be expanded, the generation of drug resistance of microorganisms is avoided, and the service life of the hand sanitizer is prolonged.
Disclosure of Invention
Aiming at the technical problems of the hand sanitizer, the invention provides the probiotic extract-containing compounded polyhexamethylene biguanide salt no-wash hand sanitizer which is simple in method, convenient to operate, capable of effectively achieving the effects of enhancing the sterilization effect and expanding the antibacterial spectrum, and the preparation method thereof.
In order to achieve the purpose, the invention adopts the technical scheme that the invention provides a probiotic extract-containing compounded polyhexamethylene biguanide salt no-clean hand sanitizer, which comprises the following effective components in percentage by weight:
the pH regulator is used for regulating the pH value of the hand sanitizer to weak acidity, and the probiotic fermentation product is supernatant obtained after probiotic fermentation and separation.
Preferably, the PH adjusting agent is citric acid.
Preferably, the plant extract is aloe vera extract.
The invention also provides a method for preparing the probiotic extract-containing compound polyhexamethylene biguanide salt no-clean hand sanitizer, which comprises the following effective steps:
a. firstly, extracting supernatant after probiotic fermentation is finished to be used as a probiotic fermentation product;
b. adding the extracted supernatant, the plant extract, the medical glycerol and the polyhexamethylene biguanide hydrochloride into water, adding a pH regulator while stirring, and regulating to weak acidity;
c. and adding the adjusted materials into a homogenizer for homogenization to obtain the probiotic extract-containing compounded polyhexamethylene biguanide salt no-wash hand sanitizer.
Preferably, the step a comprises the following effective steps:
a1, strain activation: putting the milk powder culture medium into an autoclave for sterilization, and inoculating probiotics preserved in a glycerin pipe from a refrigerator into the milk powder culture medium for fermentation for 24 hours after sterilization;
a2, seed liquid culture: uniformly mixing the seed liquid culture medium with the auxiliary materials of milk powder, mixing in a 250ml triangular flask, inoculating the activated strain into the culture medium, and fermenting for 24 hours;
a3, 10L seed fermentation: preparing 10L of milk powder culture medium, sterilizing in an autoclave, inoculating seed liquid, and fermenting for 24 h;
a4, 500L seed fermentation: preparing 500L of milk powder culture medium, placing the milk powder culture medium into an autoclave for sterilization, and then inoculating the seed liquid obtained in the step a3 for fermentation for 24 hours;
a5, and collecting probiotic fermentation extract: after the fermentation is finished, putting the fermentation liquor into a centrifugal machine, wherein the centrifugal speed is 12000rpm/min, and collecting supernatant;
and a6, pumping the collected supernatant into membrane filtration equipment for filtration to obtain a probiotic fermentation product.
Preferably, the probiotic is a lactic acid bacterium.
Preferably, in the step b, the pH is adjusted to 6.5.
Compared with the prior art, the invention has the advantages and positive effects that,
1. the invention provides a probiotic extract-containing compounded polyhexamethylene biguanide salt no-wash hand sanitizer and a preparation method thereof, wherein a probiotic fermentation product is creatively compounded with polyhexamethylene biguanide hydrochloride and medical glycerol, so that the obtained hand sanitizer not only has the effects of long-acting sterilization and bacteriostasis, but also has the effects of moisturizing and moistening hand skin, and meanwhile, the invention utilizes supernatant liquid obtained during probiotic fermentation as a raw material, thereby avoiding resource waste and achieving the purposes of environmental protection and energy conservation.
Detailed Description
In order that the above objects, features and advantages of the present invention can be more clearly understood, the present invention will be further described with reference to the following examples. It should be noted that the embodiments and features of the embodiments of the present application may be combined with each other without conflict.
In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention, however, the present invention may be practiced in other ways than those specifically described herein, and thus the present invention is not limited to the specific embodiments of the present disclosure.
Example 1, the probiotic extract-containing compounded polyhexamethylene biguanide salt no-wash hand sanitizer provided in this example
Firstly, preparing a probiotic fermented extract, putting a milk powder culture medium into an autoclave for sterilization, and inoculating lactic acid bacteria stored in a glycerin pipe from a refrigerator into the milk powder culture medium for fermentation for 24 hours after sterilization.
Then, preparing a seed liquid culture medium, mixing the seed liquid culture medium with the auxiliary materials of milk powder uniformly in a 250ml triangular flask, inoculating the activated strain into the culture medium, and fermenting for 24 hours.
Fermenting 10L of seeds, preparing 10L of milk powder culture medium, sterilizing in an autoclave, inoculating seed liquid, and fermenting for 24 h;
fermenting 500L of seeds, preparing 500L of milk powder culture medium, sterilizing in an autoclave, inoculating 10L of seed liquid, fermenting for 24h, and separating after fermentation.
Collecting lactobacillus fermentation extract, placing fermentation liquor into a centrifuge at the centrifugal speed of 12000rpm/min after fermentation is finished, and collecting supernatant.
And pumping the collected supernatant into membrane filtration equipment for filtration to obtain a lactic acid bacteria fermentation product. The supernatant is generally used as wastewater, some of the supernatants can be used as a feed additive, the main components of the supernatant are a small amount of natural lactic acid and most of metabolic products, the supernatant generated during fermentation and separation of lactic acid bacteria is added into the hand sanitizer after research, the moisture retention time of the hand sanitizer can be effectively prolonged to 8-9 hours, the traditional long-acting moisture retention time is generally about 5-6 hours, and the obtained hand sanitizer is mild in texture and small in damage to skin through the addition of lactic acid bacteria fermentation products, and is suitable for delicate skin of children.
After the lactobacillus fermentation product is extracted, 20% of extracted supernatant, 2.5% of plant extract, 1% of medical glycerol and 0.4% of polyhexamethylene biguanide hydrochloride are added into water, 0.05% of pH regulator is added while stirring, the pH is adjusted to 6.5, sweat secreted by a human body is acidic, dirt on skin can be acidic due to inorganic salt separated out along with the sweat and grease secreted by the skin surface, if the absorption liquid is alkaline, the two are neutralized, skin wrinkles are caused, and the weak acidity is favorable for keeping the skin soft.
And adding the adjusted materials into a homogenizer for homogenization to obtain the probiotic extract-containing compounded polyhexamethylene biguanide salt no-wash hand sanitizer, and then conveying the hand sanitizer into a filling tank for filling.
Example 2, this example provides a non-washing hand sanitizer containing probiotic extract compounded with polyhexamethylene biguanide salt
The preparation method provided by this example is the same as that in example 1, and mainly includes different proportions of 10% of probiotic fermentation product, 5% of plant extract, 0.8% of medical glycerin, 0.25% of polyhexamethylene biguanide hydrochloride, 0.03% of PH regulator, and the balance of water.
Example 3 this example provides a polyhexamethylene biguanide salt leave-on hand sanitizer without probiotic extract
The preparation method provided by this example is the same as that in example 1, and mainly includes, in different proportions, 5% of a plant extract, 0.8% of medical glycerin, 0.25% of polyhexamethylene biguanide hydrochloride, 0.03% of a PH adjuster, and the balance water.
And (3) sterilization test:
the sterilization test is regulated by 2.1.1.5.5 and 2.1.1.9.3 in the disinfection technical Specification of the Ministry of health (2002), the action temperature is controlled by water bath at 19-21 ℃, the test is repeated for 3 times, and 3 times of repeated tests show that:
TABLE 1 Sterilization test results
According to the requirements of the hygienic standard GB15979-2002 of the disposable hygienic product, the sterilization rate is more than or equal to 90 percent, namely the killing logarithm value is more than or equal to 1, and the product has antibacterial performance; the sterilization rate is more than or equal to 99.999 percent, namely the killing log value is more than or equal to 5.00, which indicates that the product has the disinfection performance. The disinfectant of the embodiment 1-2 is a guanidine-containing disinfectant, and the action time is required to be more than 10min to achieve the killing logarithm value of more than or equal to 5 (namely, the sterilization rate is more than or equal to 99.999%). Meanwhile, the effect of promoting the lactic acid bacteria fermentation product can be clearly observed compared to example 3.
The disinfecting solutions of the respective examples were subjected to stability tests, respectively. The polyhexamethylene biguanide in the sample stock solution is respectively measured, the sample stock solution is stored for 3 months at 37 ℃, the amount of the polyhexamethylene biguanide in each sample is measured again, the reduction rate of the effective components of the examples 1-2 is less than or equal to 10 percent and is about 8 percent, the requirements of disinfection technical specification (2002 edition) -2.2.3.2.1 are met, and the storage period of the samples can be determined to be 2 years. The reduction rate of the effective components in example 3 is 9.5%, and the detection shows that the arsenic content of the sample is less than 0.05mg/kg, the lead content is less than 0.05mg/kg, and the mercury content is less than 0.05mg/kg, so that the standard requirements of technical standards for cosmetic safety (2015 edition) are met. Therefore, the lactic acid bacteria fermentation product has the effect of inhibiting the decrease of the effective components.
Moisture retention test:
the hand surfaces of four people are cleaned by selecting a certain brand of wash-free moisturizing hand sanitizer commonly used in the market and the hand sanitizers prepared in the embodiments 1 to 3 for three times, and then the palms of the four people are placed in the same environment to observe the moisturizing time of the hand surfaces.
TABLE 2 moisturizing test results
Through the above tests, it was confirmed that the lactic acid bacteria fermentation product had an effect of significantly improving the moisturizing effect.
DPPH radical scavenging test
The determination of DPPH free radical clearance rate by the hand sanitizer is evaluated by referring to T/SHRH 006-2018. The evaluation results were as follows:
TABLE 3 measurement results of DPPH radical scavenging rate
Through the tests, the DPPH free radical clearance rate of the hand sanitizer provided by the invention is more than or equal to 75%, and the lactic acid bacteria fermentation product has the effect of improving the DPPH free radical clearance rate.
The above description is only a preferred embodiment of the present invention, and not intended to limit the present invention in other forms, and any person skilled in the art may apply the above modifications or changes to the equivalent embodiments with equivalent changes, without departing from the technical spirit of the present invention, and any simple modification, equivalent change and change made to the above embodiments according to the technical spirit of the present invention still belong to the protection scope of the technical spirit of the present invention.
Claims (7)
1. The probiotic extract-containing compound polyhexamethylene biguanide salt no-clean hand sanitizer is characterized by comprising the following effective components in percentage by weight:
the pH regulator is used for regulating the pH value of the hand sanitizer to weak acidity, and the probiotic fermentation product is supernatant obtained after probiotic fermentation and separation.
2. The probiotic extract-containing compounded polyhexamethylene biguanide salt no-wash hand sanitizer according to claim 1, wherein the PH regulator is citric acid.
3. The probiotic extract-containing compounded polyhexamethylene biguanide salt hand sanitizer according to claim 1, wherein the plant extract is aloe vera extract.
4. The method for preparing the probiotic extract-containing compounded polyhexamethylene biguanide salt no-clean hand sanitizer according to any one of claims 1 to 3 is characterized by comprising the following effective steps:
a. firstly, extracting supernatant after probiotic fermentation is finished to be used as a probiotic fermentation product;
b. adding the extracted supernatant, the plant extract, the medical glycerol and the polyhexamethylene biguanide hydrochloride into water, adding a pH regulator while stirring, and regulating to weak acidity;
c. and adding the adjusted materials into a homogenizer for homogenization to obtain the probiotic extract-containing compounded polyhexamethylene biguanide salt no-wash hand sanitizer.
5. The preparation method of the probiotic extract-containing compounded polyhexamethylene biguanide salt no-wash hand sanitizer according to claim 4, wherein the step a comprises the following effective steps:
a1, strain activation: putting the milk powder culture medium into an autoclave for sterilization, and inoculating probiotics preserved in a glycerin pipe from a refrigerator into the milk powder culture medium for fermentation for 24 hours after sterilization;
a2, seed liquid culture: uniformly mixing the seed liquid culture medium with the auxiliary materials of milk powder, mixing in a 250ml triangular flask, inoculating the activated strain into the culture medium, and fermenting for 24 hours;
a3, 10L seed fermentation: preparing 10L of milk powder culture medium, sterilizing in an autoclave, inoculating seed liquid, and fermenting for 24 h;
a4, 500L seed fermentation: preparing 500L of milk powder culture medium, placing the milk powder culture medium into an autoclave for sterilization, and then inoculating the seed liquid obtained in the step a3 for fermentation for 24 hours;
a5, and collecting probiotic fermentation extract: after the fermentation is finished, putting the fermentation liquor into a centrifugal machine, wherein the centrifugal speed is 12000rpm/min, and collecting supernatant;
and a6, pumping the collected supernatant into membrane filtration equipment for filtration to obtain a probiotic fermentation product.
6. The preparation method of the probiotic extract-containing compounded polyhexamethylene biguanide salt leave-in hand sanitizer according to claim 5, wherein the probiotics are lactic acid bacteria.
7. The method for preparing the probiotic extract-containing compounded polyhexamethylene biguanide salt no-wash hand sanitizer according to claim 6, wherein in the step b, the pH is adjusted to 6.5.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110535895.9A CN113081945A (en) | 2021-05-17 | 2021-05-17 | Probiotics extract-containing compound polyhexamethylene biguanide salt no-wash hand sanitizer and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110535895.9A CN113081945A (en) | 2021-05-17 | 2021-05-17 | Probiotics extract-containing compound polyhexamethylene biguanide salt no-wash hand sanitizer and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113081945A true CN113081945A (en) | 2021-07-09 |
Family
ID=76665886
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110535895.9A Pending CN113081945A (en) | 2021-05-17 | 2021-05-17 | Probiotics extract-containing compound polyhexamethylene biguanide salt no-wash hand sanitizer and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113081945A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106309309A (en) * | 2016-08-23 | 2017-01-11 | 江苏微康生物科技有限公司 | Milk fermented product filtrate as well as preparation method and application thereof |
| CN106727263A (en) * | 2016-11-22 | 2017-05-31 | 深圳弘睿康生物科技有限公司 | Skin Tiny ecosystem improves preparation and preparation method thereof |
| CN111534455A (en) * | 2020-04-02 | 2020-08-14 | 广州栋方生物科技股份有限公司 | Preparation of lactobacillus sporolysis product and application of lactobacillus sporolysis product in cosmetics |
| CN111544371A (en) * | 2020-06-12 | 2020-08-18 | 洋浦吉商生物科技有限公司 | Whitening and repairing mask containing probiotic active extracting solution and nicotinamide |
| CN111544337A (en) * | 2020-06-17 | 2020-08-18 | 广东中科天元新能源科技有限公司 | Hyaluronic acid-containing moisturizing and skin-moistening no-wash gel disinfectant and preparation method thereof |
-
2021
- 2021-05-17 CN CN202110535895.9A patent/CN113081945A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106309309A (en) * | 2016-08-23 | 2017-01-11 | 江苏微康生物科技有限公司 | Milk fermented product filtrate as well as preparation method and application thereof |
| CN106727263A (en) * | 2016-11-22 | 2017-05-31 | 深圳弘睿康生物科技有限公司 | Skin Tiny ecosystem improves preparation and preparation method thereof |
| CN111534455A (en) * | 2020-04-02 | 2020-08-14 | 广州栋方生物科技股份有限公司 | Preparation of lactobacillus sporolysis product and application of lactobacillus sporolysis product in cosmetics |
| CN111544371A (en) * | 2020-06-12 | 2020-08-18 | 洋浦吉商生物科技有限公司 | Whitening and repairing mask containing probiotic active extracting solution and nicotinamide |
| CN111544337A (en) * | 2020-06-17 | 2020-08-18 | 广东中科天元新能源科技有限公司 | Hyaluronic acid-containing moisturizing and skin-moistening no-wash gel disinfectant and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103356738B (en) | Skin disinfection gel and its application | |
| CN102511507A (en) | Marine bios sterilization solution | |
| CN102423497A (en) | Marine organism degerming air refresher | |
| CN102113981B (en) | Non-rinse pregnant woman perineum medical care gel and preparation method thereof | |
| CN101658397A (en) | Functional wipes containing chlorine dioxide and application thereof | |
| CN101708289A (en) | Preparation method for bamboo vinegar antibacterial liquid | |
| CN107737070A (en) | Sterilization, skin care hand cleaning liquid | |
| CN112137913A (en) | Washing-free disinfection gel and preparation method thereof | |
| CN103053626B (en) | Natural lemon disinfectant and preparation method thereof | |
| CN101543658B (en) | Cervical cap for preventing and treating cervical erosion and preparation method thereof | |
| CN101766186A (en) | Composition capable of slowly-releasing chlorine dioxide and application thereof | |
| CN112741087A (en) | Cold chain food disinfectant and preparation method thereof | |
| CN101803611B (en) | Citric acid iodine disinfectant and method for preparing same | |
| CN113100255A (en) | Composite nano-silver pet deodorizing disinfectant and preparation method thereof | |
| CN112675086A (en) | Passion fruit hand-washing-free gel hand sanitizer and preparation method thereof | |
| CN111671816A (en) | Plant type no-clean antibacterial disinfectant and preparation method thereof | |
| CN102948429A (en) | Livin bacteria removing liquid for marine organism | |
| CN109498535B (en) | Bacteriostatic composition, bacteriostatic no-wash gel, and preparation method and application thereof | |
| CN109350570B (en) | Cosmetic preservative based on natural plant components and application thereof | |
| CN106962420A (en) | A kind of disposable thimerosal based on plant extracts and preparation method thereof | |
| CN113081945A (en) | Probiotics extract-containing compound polyhexamethylene biguanide salt no-wash hand sanitizer and preparation method thereof | |
| CN107669508B (en) | Preparation process of antibacterial wet tissue | |
| CN106359558A (en) | Natural cherry fresh keeping agent and preparation method thereof | |
| CN113080207B (en) | Process for preparing disinfectant | |
| CN107536764A (en) | A kind of composition with antisepsis and sterilization effect and its application in cosmetics |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |