CN106727263A - Skin Tiny ecosystem improves preparation and preparation method thereof - Google Patents
Skin Tiny ecosystem improves preparation and preparation method thereof Download PDFInfo
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- CN106727263A CN106727263A CN201611027229.XA CN201611027229A CN106727263A CN 106727263 A CN106727263 A CN 106727263A CN 201611027229 A CN201611027229 A CN 201611027229A CN 106727263 A CN106727263 A CN 106727263A
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- probiotics
- tiny ecosystem
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- 238000002360 preparation method Methods 0.000 title claims abstract description 52
- 239000006041 probiotic Substances 0.000 claims abstract description 87
- 235000018291 probiotics Nutrition 0.000 claims abstract description 87
- 239000006228 supernatant Substances 0.000 claims description 50
- 239000000843 powder Substances 0.000 claims description 31
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 28
- 230000000529 probiotic effect Effects 0.000 claims description 27
- 230000001954 sterilising effect Effects 0.000 claims description 16
- 150000004676 glycans Chemical class 0.000 claims description 15
- 229920001282 polysaccharide Polymers 0.000 claims description 15
- 239000005017 polysaccharide Substances 0.000 claims description 15
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 14
- 239000008103 glucose Substances 0.000 claims description 14
- 239000004310 lactic acid Substances 0.000 claims description 14
- 235000014655 lactic acid Nutrition 0.000 claims description 14
- HQKMJHAJHXVSDF-UHFFFAOYSA-L magnesium stearate Chemical compound [Mg+2].CCCCCCCCCCCCCCCCCC([O-])=O.CCCCCCCCCCCCCCCCCC([O-])=O HQKMJHAJHXVSDF-UHFFFAOYSA-L 0.000 claims description 12
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims description 11
- 240000001046 Lactobacillus acidophilus Species 0.000 claims description 11
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims description 11
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 claims description 11
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims description 11
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- 239000008267 milk Substances 0.000 claims description 7
- 210000004080 milk Anatomy 0.000 claims description 7
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- 238000005119 centrifugation Methods 0.000 claims description 5
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- 239000006152 selective media Substances 0.000 claims description 5
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- 229940041514 candida albicans extract Drugs 0.000 claims description 4
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- 239000012138 yeast extract Substances 0.000 claims description 4
- 241000628997 Flos Species 0.000 claims description 3
- 241000186866 Lactobacillus thermophilus Species 0.000 claims description 3
- 239000001888 Peptone Substances 0.000 claims description 3
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- 241000698291 Rugosa Species 0.000 claims description 3
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims description 3
- 239000008367 deionised water Substances 0.000 claims description 3
- 229910021641 deionized water Inorganic materials 0.000 claims description 3
- 235000019319 peptone Nutrition 0.000 claims description 3
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- 241000218588 Lactobacillus rhamnosus Species 0.000 claims description 2
- 241001608472 Bifidobacterium longum Species 0.000 claims 1
- 240000000111 Saccharum officinarum Species 0.000 claims 1
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- 229940009291 bifidobacterium longum Drugs 0.000 claims 1
- 239000003826 tablet Substances 0.000 abstract description 35
- 238000000034 method Methods 0.000 abstract description 16
- 239000000047 product Substances 0.000 abstract description 16
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- 230000003020 moisturizing effect Effects 0.000 abstract description 13
- 239000002253 acid Substances 0.000 abstract description 11
- 230000008569 process Effects 0.000 abstract description 8
- 238000003860 storage Methods 0.000 abstract description 6
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- 241000894006 Bacteria Species 0.000 description 48
- 239000000243 solution Substances 0.000 description 24
- UIIMBOGNXHQVGW-UHFFFAOYSA-M sodium bicarbonate Substances [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 21
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- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 15
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- 238000006386 neutralization reaction Methods 0.000 description 10
- 238000012360 testing method Methods 0.000 description 10
- 230000003139 buffering effect Effects 0.000 description 9
- 239000002537 cosmetic Substances 0.000 description 9
- 241000901050 Bifidobacterium animalis subsp. lactis Species 0.000 description 8
- 229940009289 bifidobacterium lactis Drugs 0.000 description 8
- 239000007788 liquid Substances 0.000 description 8
- 239000003513 alkali Substances 0.000 description 7
- 230000001580 bacterial effect Effects 0.000 description 7
- 230000008901 benefit Effects 0.000 description 7
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 6
- 230000014509 gene expression Effects 0.000 description 6
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- 241000196324 Embryophyta Species 0.000 description 3
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- 239000000463 material Substances 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- MCSXGCZMEPXKIW-UHFFFAOYSA-N 3-hydroxy-4-[(4-methyl-2-nitrophenyl)diazenyl]-N-(3-nitrophenyl)naphthalene-2-carboxamide Chemical compound Cc1ccc(N=Nc2c(O)c(cc3ccccc23)C(=O)Nc2cccc(c2)[N+]([O-])=O)c(c1)[N+]([O-])=O MCSXGCZMEPXKIW-UHFFFAOYSA-N 0.000 description 2
- 241000193830 Bacillus <bacterium> Species 0.000 description 2
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- 241000235342 Saccharomycetes Species 0.000 description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
- 229930006000 Sucrose Natural products 0.000 description 2
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- 238000004321 preservation Methods 0.000 description 2
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- KDYFGRWQOYBRFD-UHFFFAOYSA-N succinic acid Chemical compound OC(=O)CCC(O)=O KDYFGRWQOYBRFD-UHFFFAOYSA-N 0.000 description 2
- 239000005720 sucrose Substances 0.000 description 2
- 238000013268 sustained release Methods 0.000 description 2
- 239000012730 sustained-release form Substances 0.000 description 2
- BJEPYKJPYRNKOW-REOHCLBHSA-N (S)-malic acid Chemical compound OC(=O)[C@@H](O)CC(O)=O BJEPYKJPYRNKOW-REOHCLBHSA-N 0.000 description 1
- HLLSOEKIMZEGFV-UHFFFAOYSA-N 4-(dibutylsulfamoyl)benzoic acid Chemical compound CCCCN(CCCC)S(=O)(=O)C1=CC=C(C(O)=O)C=C1 HLLSOEKIMZEGFV-UHFFFAOYSA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 244000131522 Citrus pyriformis Species 0.000 description 1
- FEWJPZIEWOKRBE-JCYAYHJZSA-N Dextrotartaric acid Chemical compound OC(=O)[C@H](O)[C@@H](O)C(O)=O FEWJPZIEWOKRBE-JCYAYHJZSA-N 0.000 description 1
- 229920002444 Exopolysaccharide Polymers 0.000 description 1
- 206010016807 Fluid retention Diseases 0.000 description 1
- 241000186660 Lactobacillus Species 0.000 description 1
- 244000131316 Panax pseudoginseng Species 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
- 241000191967 Staphylococcus aureus Species 0.000 description 1
- FEWJPZIEWOKRBE-UHFFFAOYSA-N Tartaric acid Natural products [H+].[H+].[O-]C(=O)C(O)C(O)C([O-])=O FEWJPZIEWOKRBE-UHFFFAOYSA-N 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- BJEPYKJPYRNKOW-UHFFFAOYSA-N alpha-hydroxysuccinic acid Natural products OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 235000015278 beef Nutrition 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
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- 229910000019 calcium carbonate Inorganic materials 0.000 description 1
- 230000019522 cellular metabolic process Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 210000004087 cornea Anatomy 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 150000004985 diamines Chemical class 0.000 description 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
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- 239000002085 irritant Substances 0.000 description 1
- 231100000021 irritant Toxicity 0.000 description 1
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- 238000002386 leaching Methods 0.000 description 1
- 235000015122 lemonade Nutrition 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 239000001630 malic acid Substances 0.000 description 1
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- 229940099596 manganese sulfate Drugs 0.000 description 1
- 239000011702 manganese sulphate Substances 0.000 description 1
- 235000007079 manganese sulphate Nutrition 0.000 description 1
- BZDIAFGKSAYYFC-UHFFFAOYSA-N manganese;hydrate Chemical compound O.[Mn] BZDIAFGKSAYYFC-UHFFFAOYSA-N 0.000 description 1
- 238000012009 microbiological test Methods 0.000 description 1
- 230000000474 nursing effect Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 238000005498 polishing Methods 0.000 description 1
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 1
- 229920000053 polysorbate 80 Polymers 0.000 description 1
- 235000013406 prebiotics Nutrition 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 108010009004 proteose-peptone Proteins 0.000 description 1
- 210000001732 sebaceous gland Anatomy 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 210000004927 skin cell Anatomy 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
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- 238000011105 stabilization Methods 0.000 description 1
- 238000007655 standard test method Methods 0.000 description 1
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- 229960005137 succinic acid Drugs 0.000 description 1
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- 208000024891 symptom Diseases 0.000 description 1
- 239000011975 tartaric acid Substances 0.000 description 1
- 235000002906 tartaric acid Nutrition 0.000 description 1
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 1
- 238000004448 titration Methods 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/19—Cosmetics or similar toiletry preparations characterised by the composition containing inorganic ingredients
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/36—Carboxylic acids; Salts or anhydrides thereof
- A61K8/365—Hydroxycarboxylic acids; Ketocarboxylic acids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Dermatology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Emergency Medicine (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Cosmetics (AREA)
Abstract
Improve preparation the invention discloses a kind of skin Tiny ecosystem, be dissolved in skin base solution by probiotics tablets and constituted, the skin Tiny ecosystem contains 1 × 10 in improving preparation6~1 × 108CFU/g active probiotics；The pH that the skin Tiny ecosystem improves preparation is 5.0~5.6；And preparation method thereof.The present invention uses probiotics fermention culture supernatant as the skin base solution of probiotics effervescent tablet, while adding NaHCO in compressing tablet3Buffer, can maintain weak acid environment, reduce the injury of the residual for skin of alkaline face cleaning class product.Simultaneously, Tiny ecosystem of the invention improves preparation and can guarantee that in production and product storage process probiotics is active and quantity stability, the final microecological balance for fundamentally adjusting skin, the Maintenance Of Skin Health, there is moisturizing simultaneously, skin softening, the multiple-effect repair function such as compacted, and new thinking is provided to solve skin problem.
Description
Technical field
Improve preparation the present invention relates to technical field of skin care, especially a kind of skin Tiny ecosystem, and preparation method thereof.
Background technology
Influence of the microorganism to skin, beauty industry the few concern, but with the progress of life science, medical science, biology
The quality that area research person has found microorganism and has vital effect, human body skin to human body and life has with microorganism
Inseparable relation.Microecological balance is recovered skin microbial and reconstruction plays a crucial role, simultaneously for biological barrier
Constitute, the decomposition of sebaceous glands, acid cover has been formed and its important effect.Skin microbial group and host, keep association between environment
Adjust, physiological dynamic balance, it is beneficial to host, and this balance is exactly skin microecological balance.Due to skin itself,
The reason for environment, medicine, their Tiny ecosystem can often lack of proper care, and can be allowed to extensive from extraneous probiotic supplemented or the prebiotic factor
It is multiple.
Growth and breeding, secretion cornea and exocellular polysaccharide that probiotics will play cleaning skin, suppress skin surface harmful bacteria
Help the skin-care effects such as protecting skin, it is necessary to meet two preconditions, one is active probiotics, i.e., in the form of viable bacteria
Add and be present in product；Two is enough active probiotic quantity, i.e., certain viable bacteria content just can guarantee that active probiotic
Skin effect.
1st, Chinese patent：Cosmetics (CN1183266A) containing microecologic regulator, it is characterised in that the cosmetics
It is contained within youth's Bick Candida and/or deep red saccarom ycete or its metabolin of percentage by weight 1%~10%.By implementing this
Inventive technique scheme, can further improve skin care, foster skin, strong skin and the promotion skin cell metabolism effect of cosmetics
Really, and for skin provides a kind of indispensable normal physiological bacterium, reach and safeguard skin microecological balance.But exist following
Shortcoming：1. saccharomycete is not the resident bacterium of skin, it is impossible to colonized on skin, so the metabolism of skin cannot be participated in, and only
Having continual supply could work to skin, be further improved.2. youth's Bick Candida for using and dark red
Saccharomycete is dead bacterium, it is impossible to which further growth is bred.3. gram Candida and deep red saccarom ycete do not reach probiotic oral
Rank, so directly application facial skin, may destroy the physiology circulation of human body.
2nd, Chinese patent：A kind of cosmetics (CN102755281A) containing microecologic regulator, in described cosmetics
In the Bifidobacterium containing part by weight 0.5%~1%, using Bifidobacterium as cosmetics microecologic regulator, production
Tiny ecosystem skin care item out can promote skin metabolism, reach the effect of skin-protecting face nursing；But have the following disadvantages：1. used by
Bifidobacterium be one plant of bifidobacterium adolescentis, its physiological metabolism is more single.2. bifidobacterium adolescentis is mainly enteron aisle
Probiotics, it is more difficult on skin to colonize.3. the bifidobacterium adolescentis for using is mainly dead bacterium, it is impossible to which further growth is bred, ginseng
Circulated with Human Physiology.
3rd, Chinese patent：Beauty method, shield and dry thalline (CN103945829A) at skin composition, thalline, described
Invention provides a kind of MRSE obtained from the resident microbe to screen of human body surface, and in the same of sampling object
Body carries out the input and surface growth of obtained bacterium, so as to improve the skin care mode of skin health state；But there is following lacking
Point：1. bacterial strain has specificity, without the MRSE to strain level, it is impossible to determine if it is beneficial bacterium, risk
It is too big.2nd, MRSE and staphylococcus aureus are only distinguished by selective medium and differentiate, discrimination is inadequate.3rd, skin care
Although mode is personalized, operation is lengthy and tedious, and technique productions are more difficult.
4th, Chinese patent：A kind of activated probiotic skin cream and preparation method thereof (CN103251547A), in the patent
In, the active probiotic bacterium solution containing viable bacteria amount high for being easily dispersed in emulsification system is prepared using bacterium mud, glycerine and trehalose.Secondly
Determine adding conditional and addition of the active probiotic bacterium solution in face cream preparation technology, it is ensured that face cream figure stabilization with
And containing viable bacteria amount high so as to have skin care benefit skin effect；But have the following disadvantages：1：The preservative Nepal first of addition 0.1%
Fat, is unfavorable for skin health.2nd, activated probiotic skin cream the being of no curative effect property data for being obtained；3rd, the shelf-life is short.
5th, Chinese patent：A kind of microbe effervescent tablet and preparation method thereof (CN103585184B), in the patent in question, carries
The method for having supplied to prepare active microorganism effervescent tablet, effectively extends micro- in the auxiliary material addition effervescent tablet that will protect microbial activity
The preservation time of bio-effervescent tablets；But acid source used is that lemonade, lactic acid, tartaric acid, butanedioic acid, malic acid etc. are artificial synthesized
Lactic acid, be not natural fermentation products.And disintegration time is in 2.0~3.0min, sustained release is not belonging to, increases microorganism and adding
Work, using, storage during inactivate risk.
Current application of the probiotics in skin care item there are problems that following：Probiotics exists with particle or pulvis, absolutely
Most of is dead bacterium, it is impossible to carry out metabolism；Probiotics cannot for a long time stop on skin；Probiotics does not can be uniformly dispersed
In frost or newborn system；Contain preservative in most cosmetics, increased potential safety hazard and be unfavorable for that probiotics survives.Cause
And active probiotic skin effect and not fully up to expectations.
The content of the invention
The present invention in view of the shortcomings of the prior art, proposes that a kind of skin Tiny ecosystem improves preparation, and pollution-free, effect is good.
In order to realize foregoing invention purpose, the present invention provides following technical scheme：A kind of skin Tiny ecosystem improves preparation, by
Probiotics tablets are constituted in being dissolved in skin base solution, and the skin Tiny ecosystem contains 1 × 10 in improving preparation6~1 × 108CFU/g live probiotics
Bacterium；The pH that the skin Tiny ecosystem improves preparation is 5.0~5.6.
Preferably, the active probiotic is lactobacillus acidophilus, L. casei casei, Lactobacillus rhamnosus, length pair
The mixing of one or more arbitrary proportion in discrimination bacillus and lactobacillus thermophilus.
Preferably, the skin base solution is the supernatant obtained by the active probiotic is centrifuged after fermented and cultured.
Preferably, lactic acid and exocellular polysaccharide are contained in the skin base solution.
A kind of skin Tiny ecosystem as described above improves the preparation method of preparation, comprises the following steps：
(1), being inoculated into probiotics carries out 37~40 DEG C of 17~24h of quiescent culture in exocellular polysaccharide selective medium,
12000~18000rpm is centrifuged 3~10min, obtains probiotic powder and supernatant；After taking 90~95 DEG C of 3~7min of sterilizing of supernatant
Room temperature is cooled to, skin base solution is obtained；
(2), the titratable acidity and pH value of determination step (1) skin base solution, is calculated the NaHCO needed for neutralizing skin base solution3
Amount, mixes with step (1) probiotic powder, and tablet conven-tional adjuvants, and compressing tablet obtains probiotics tablets, viable bacteria in each probiotics tablets
Number is at least 1 × 109CFU/g。
Preferably, the tablet conven-tional adjuvants include trehalose, glucose, sucrose, milk powder, pearl powder, Flos Rosae Rugosas pollen
And magnesium stearate.
Preferably, the exocellular polysaccharide selective medium is：Skimmed milk powder 5%, glucose 5%, yeast extract
0.35%, peptone 0.35%, deionized water 89.3% adjusts pH6.2~6.4.
Compared with prior art, the present invention has advantages below：Using probiotics fermention culture supernatant as probiotics
The acid solution of effervescent tablet, while adding NaHCO in compressing tablet3Buffer, can maintain weak acid environment, reduce alkaline face cleaning class product
Remain the injury for skin.
A certain proportion of mixing probiotics is carried out fermented and cultured, centrifuging and taking supernatant by the present invention first.This supernatant zymotic fluid
Be not only hyperalimentation fluid, containing the carbon nitrogen source needed for growth of probiotics, and especially comprising natural fermented metabolite lactic acid and
Exocellular polysaccharide.On the one hand exocellular polysaccharide has moisture-keeping function to human body skin, on the other hand there is protective agent to act on probiotics,
Probiotic active can be protected.Necessary to lactic acid is human body skin, skin on the one hand can be helped to improve the ability that alkali is neutralized, subtracted
The injury of the residual for skin of few alkalescence face cleaning class product.Meanwhile, lactic acid is natural fermentation products, is not artificial synthesized or adds
Plus,, used as effervescent tablet acid source, this is an innovation for it.
Then according to centrifuged supernatant acidity, every gram of NaHCO of centrifuged supernatant of neutralization is calculated3Amount, sets up and neutralizes public affairs
Formula and model, make whole skin care item system pH reach 5.0~5.6.Then the NaHCO that probiotic powder will be mixed and be calculated3
Matched, unclassified stores is mixed in constant humidity desinfection chamber, carries out compressing tablet, the alkali source needed for obtaining effervescent tablet.In order to continue to carry
For the carbon source needed for growth of probiotics, glucose is contained in tablet material.Trehalose is additionally added in tablet, trehalose is a kind of guarantor
Shield agent, on the one hand has efficient biological protective action to probiotics strain cell, can help the extraneous severe ring of biological resistance
Border, and then probiotic active is improved, on the other hand there is the characteristics such as anti-aging, moisturizing, after-sun to skin.Each tablet
Viable count is at least up to 1 × 109CFU/g.Here probiotics is lactobacillus acidophilus, L. casei casei, newborn bifid
Bacillus and streptococcus thermophilus, and be one or more mixing of arbitrary proportion.
Tablet is put into skin base solution, effervescent tablet slowly discharges, and will not rapidly produce a large amount of foams, probiotics is existed
One ambient growth of amphimicrobian, helps to ensure that in storage process probiotics is active and quantity stability.Gained
Skin Tiny ecosystem improve preparation in contain 1 × 106~1 × 108The active probiotic of CFU/g.
Skin Tiny ecosystem of the invention improves preparation and can guarantee that probiotics is active in production and product storage process
With quantity stability, finally fundamentally adjust skin microecological balance, the Maintenance Of Skin Health, overcome Traditional makeup/
Skin care item are cured the symptoms, not the disease and are also easy to produce the defect of side effect, while have moisturizing, skin softening, and the multiple-effect repair function such as compact, and is
Solve skin problem and provide new thinking.Its preparation is by scientific algorithm reaction system and mixed proportion, farthest
Maintain the active ingredient of each function ingredients.
In this patent active probiotic effervescent tablet sustained release and protective agent in the presence of, exposed to it is considered that easily inactivation
Aqueous systems in.Effervescent tablet type of service：On the one hand can effectively mix probiotics, be uniformly distributed it；On the other hand, effervescent tablet
A large amount of CO can be produced2, make environment in anaerobism form, the growth of probiotics of amphimicrobian is greatly facilitated in survival, it is ensured that benefit
The activity and stability of raw bacterium.Skin Tiny ecosystem improves preparation cosmetics different from the past, and its skin base solution is that probiotics naturally sends out
What ferment was obtained, it is limited not only containing the skin care material such as lactic acid and exocellular polysaccharide, and containing the carbon nitrogen source needed for growth of probiotics
Easily decline is inactivated in aqueous systems to prevent probiotics, with stability and activity higher.
So one aspect of the present invention introduces effervescent tablet in terms of Tabules, liquid or some solid actives benefit can be reduced
Inactivation risk of the raw bacterium during processing, storage, use, is finally reached suppression harmful bacteria, and balance skin surface p H, moisturizing is protected
Wet effect.On the other hand, solve to be easy to decline inactivation in the system that probiotics exists exposed to a large amount of free waters, viable bacteria is steady
Qualitative low, viable bacteria is difficult the technical problem such as dispersed, of the invention in the form of alkali source tablet adds natural fermented nutrient solution, and
And whole system is maintained in the range of pH5.0~5.6 so that this preparation is to application on human skin more Safe beneficial.
NaHCO in alkali source3There are certain buffering effect, NaHCO3The neutralizing effect of generation is not to neutralize H completely+, but
Buffer system is set up in N-process, such balance is had in final system：
It is that after adding bacterial strain, on the one hand can enter row buffering, one to the product acid of bacterial strain to set up such equilibrium system benefit
Aspect can also constantly discharge CO as bacterial strain produces acid2, make probiotics in an ambient growth for amphimicrobian, help to protect
Probiotics is active in storage process and quantity stability for card.
Brief description of the drawings
Fig. 1 is NaHCO in the present invention3Titrate the titration figure of supernatant；
Fig. 2 is NaHCO in the present invention3Titrate the neutralization ratio of supernatant and the graph of a relation of titer.
Specific embodiment
With reference to embodiment, the present invention will be described in detail, the description of this part be only it is exemplary and explanatory, no
Reply protection scope of the present invention has any restriction effect.
A kind of skin Tiny ecosystem improves preparation, including probiotics tablets and skin base solution, and probiotics tablets are dissolved in skin base solution；Skin
The viable count that Tiny ecosystem improves in preparation is 1 × 106~1 × 108CFU/g；Skin Tiny ecosystem improve the pH of preparation for 5.0~
5.6。
The preparation that skin Tiny ecosystem improves preparation is obtained using following methods：
1st, the acquisition of nutrient solution
1) activated spawn
Active probiotic is the one of lactobacillus acidophilus, L. casei casei, bifidobacterium lactis and streptococcus thermophilus
Plant or various.
Extracting lactic acid bacterium freeze-drying lactobacillus, are activated.The sterilization of lyophilized pipe is cut open, a small amount of culture medium is added, bacterium solution is suctioned out, entered
Row flat board is coated with, and 48h is cultivated under 37 DEG C of anaerobic conditions.Select and meet lactic acid bacteria form, grow good bacterium colony, carries out
Liquid seed culture medium is activated, and activation condition cultivates 12~24h under 37 DEG C of anaerobic conditions, as first order seed.By one-level kind
Son is inoculated into by the inoculum concentration of 2%~8% (v/v) and activation culture 17h is carried out in seed culture medium.
MRS Liquid Culture based formulas are as follows：
Casein peptone 10.0g, beef leaching thing 10.0g, yeast extract 5.0g, glucose 5.0g, sodium acetate 5.0g, lemon
Lemon acid diamine 2.0g, Tween 80 1.0g, dipotassium hydrogen phosphate 2.0g, epsom salt 0.2g, seven water manganese sulfate 0.05g, calcium carbonate
20.0g, distilled water 1.0L, pH6.8.Add agar 20.0g (15g of import) on the basis of MRS solid medium liquid medium withins.
118 DEG C, sterilize 30min.
2) strain fermentation
Cell concentration is reached 1 × 107CFU/g is inoculated with, and is fermented.Fermentation medium is exocellular polysaccharide selectivity ESM
(expolysaccharides selection medium)：Skimmed milk powder 5%, glucose 5%, yeast extract 0.35%,
Peptone 0.35%, deionized water 89.3% adjusts pH6.2.In 37 DEG C of quiescent culture 17h, 10,000rpm centrifugations take supernatant
Room temperature, the pH of acquisition, titratable acidity are cooled to after 90~95 DEG C of sterilizing 5min of liquid.
This centrifuged supernatant is required nutrient solution.Hyperalimentation fluid is not only, containing the carbon nitrogen source needed for growth of probiotics,
And comprising natural fermented metabolite：Lactic acid and exocellular polysaccharide.On the one hand exocellular polysaccharide has moisture-keeping function to human body skin,
On the other hand there is protective agent to act on probiotics, probiotic active can be protected.Necessary to lactic acid is human body skin, on the one hand
Skin can be helped to improve the ability that alkali is neutralized, reduce the injury of the residual for skin of alkaline face cleaning class product.Meanwhile, lactic acid
It is natural fermentation products, is not artificial synthesized or addition,, used as effervescent tablet acid source, this is an innovation for it.
2nd, alkali source NaHCO3Calculating
Every gram of NaHCO of centrifuged supernatant of neutralization is calculated according to supernatant titratable acidity3Amount, according still further to maximum addition
Different weight percentage addition NaHCO3, set up and neutralize formula and model, whole skin care item system is reached skin microbial and grow
Optimal pH 5.0~5.6.NaHCO needed for calculating3The calculating process of quality
NaHCO needed for neutralizing 1g or 1mL samples completely3Quality=titratable acidity/° T × 84 × 10-6G/g samples
Wherein, titratable acidity/° T × 84 × 10-6It is mol numbers, equivalent to NaHCO3And H+Molal quantity, also correspond to produce
CO2Molal quantity.
So according to the acidity of supernatant after sterilizing, being inserted in above-mentioned formula, being calculated to neutralize completely needs NaHCO3's
Amount.Because NaHCO3Also there is certain buffering effect, therefore buffer system is made formula by consideration, as shown in Figure 1.By Fig. 1
As can be seen that the buffer system of gained is best with the compatible degree of exponential formula.Because NaHCO3The neutralizing effect of generation is simultaneously
It is not to neutralize H completely+, but buffer system is set up in the process, such balance is had in final system：
Such equilibrium system benefit is after adding bacterial strain, on the one hand can to enter row buffering to the product acid of bacterial strain, on the one hand
CO can also be constantly discharged as bacterial strain produces acid2.The CO of generation2Be conducive to the growth of probiotics of amphimicrobian.
Bring exponential formula into, when can calculate pH5.6, corresponding titratable acidity.Due to buffer system, in drop
Determine acidity it is higher when, balance carry out in one direction, therefore, choose titratable acidity where region, carry out linear push
Lead, as shown in Figure 2.
After bringing formula into, corresponding theoretical neutralization ratio can be obtained according to titratable acidity.Counted according to theoretical neutralization ratio
Calculate, it is 5.6 or so to obtain correspondence pH.
According to the foundation of buffering formula, 1g fermented supernatant fluids neutralize the NaHCO required for reaching pH5.63Addition formula
For：
In practice with the NaHCO needed for 1g or 1mL samples3Quality=titratable acidity/° T × 84 × 10-6G/g samples × reason
By neutralization ratio
3rd, probiotics compressing tablet
Viable count will at least reach 1 × 10 in each tablet9CFU/mL, is calculated Freeze-dry Powder of Probioctics addition.This
In probiotics be lactobacillus acidophilus, L. casei casei, bifidobacterium lactis and streptococcus thermophilus, and be a kind of or many
Plant the Mixed Microbes of arbitrary proportion.The alkali source NaHCO being calculated3It is 30%~50% to measure, and glucose addition is 5~10%,
Trehalose, glucose, sucrose, milk powder, pearl powder, Flos Rosae Rugosas pollen and the remaining amount of magnesium stearate polishing.Dispensing drying and sterilizing,
Mixed in desinfection chamber, cross the sieve sieve of 40 mesh, dehumidifier compressing tablet.
The supplement of glucose, for the growth of probiotics provides sufficient carbon source.Trehalose is a kind of protective agent, on the one hand right
Probiotics strain cell has efficient biological protective action, can help the extraneous adverse circumstances of biological resistance, and then improve benefit
Raw bacterium activity, on the other hand has the characteristics such as anti-aging, moisturizing, after-sun to skin.
4th, skin Tiny ecosystem improves the acquisition of preparation
The final tablet for obtaining can reach the effect of slow release in fermented supernatant fluid, will not produce a large amount of bubbles.
Embodiment 1
1st, L. casei casei was activated into for two generations in MRS culture mediums, the thalline of logarithmic phase will be grown into, in born of the same parents
Exo polysaccharides selectivity ESM, 37 DEG C carry out High Density Cultivation 20h, after by zymotic fluid desk centrifuge 10,000rpm is centrifuged
5min, obtains supernatant.
2nd, room temperature is cooled to after 90 DEG C of sterilizing 5min of gained supernatant, determines its acidity, measure pH3.72, titratable acidity
159.54 ° of T, the supernatant that will be mixed is divided in two blue lid bottles for having sterilized.
3rd, according to centrifuged supernatant acidity after sterilizing, it is calculated every gram of NaHCO of centrifuged supernatant of theoretical neutralization3Amount is
1.34×10-2g.According to the foundation of buffering formula, 1kg fermented supernatant fluids neutralize the NaHCO required for reaching pH5.63Addition
8g NaHCO3。
4th, it is 2.4 × 10 by viable count11CFU/g bifidobacterium lactis carry out compressing tablet, are calculated the addition of 0.3g tablets bacterium powder
It is 6% to measure, and glucose addition is 30%, NaHCO3Addition is 40%, and trehalose addition is 14%, and milk powder addition is
5%, pearl powder addition is 5%.
5th, in addition to bacterium powder, other dispensing drying and sterilizings cross 40 eye mesh screens in desinfection chamber, after being well mixed, open dehumidifier.
Operating desk after dehumidifier is mixed and compressing tablet, and fermented liquid supernatant is dispensed to spray bottle.
6th, tablet is added in skin base solution, is released rapidly without a large amount of bubbles, gas uniformly slowly discharges, without bottle explosion feelings
Condition, obtaining skin Tiny ecosystem improves preparation, and number of live bacteria of probiotics is 5.4 × 106CFU/g, pH value is 5.1.
Embodiment 2
1st, lactobacillus acidophilus, L. casei casei, lactobacillus thermophilus are activated two in MRS culture mediums respectively
In generation, the thalline of logarithmic phase will be grown into, in exocellular polysaccharide selectivity ESM, 37 DEG C carry out High Density Cultivation 17h, nutrient solution viable bacteria
Number respectively reaches 1.4 × 109、5.5×109、6×109CFU/g, after by zymotic fluid desk centrifuge 12,000rpm carry out from
Heart 5min, takes supernatant.
2nd, gained supernatant is mixed according to volume ratio 3: 3: 4, is obtained probiotics supernatant.Supernatant is gone out at 95 DEG C
Room temperature is cooled to after bacterium 5min, its acidity is determined, pH3.63,183.80 ° of T of titratable acidity is measured.
3rd, according to centrifuged supernatant acidity after sterilizing, it is calculated every gram of NaHCO of centrifuged supernatant of theoretical neutralization3Amount is
1.55×10-2g.According to the foundation of buffering formula, 10kg fermented supernatant fluids neutralize the NaHCO required for reaching pH 5.63Addition
Amount 93g NaHCO3。
4th, lactobacillus acidophilus and bifidobacterium lactis are mixed according to 1: 1 ratio, total viable count is 7.9 × 1010CFU/g, meter
It is 18% that calculation obtains 0.15g tablet bacterium powders addition, and glucose addition is 10%, NaHCO3Addition is 30%, trehalose
Addition is 12%, and milk powder addition is 15%, and pearl powder addition is 10%, magnesium stearate 5%.
5th, in addition to bacterium powder, other dispensing drying and sterilizings cross 40 eye mesh screens in desinfection chamber, after being well mixed, open dehumidifier.
Operating desk after dehumidifier is mixed and compressing tablet, and fermented liquid supernatant is dispensed to spray bottle.
6th, tablet is added in skin base solution, is released rapidly without a large amount of bubbles, gas uniformly slowly discharges, without bottle explosion feelings
Condition, obtaining skin Tiny ecosystem improves preparation, and number of live bacteria of probiotics is 8 × 106CFU/g, pH value is 5.5.
Embodiment 3
1st, lactobacillus acidophilus, L. casei casei, bifidobacterium lactis are activated two in MRS culture mediums respectively
In generation, the thalline of logarithmic phase will be grown into, in exocellular polysaccharide selectivity ESM, 37 DEG C carry out High Density Cultivation, nutrient solution viable count point
Do not reach 5.5 × 109、4×109、9×109CFU/g, after zymotic fluid desk centrifuge 9,000rpm are carried out into centrifugation 15min,
Take supernatant.
2nd, gained supernatant is mixed according to volume ratio 1: 3: 3, to probiotics supernatant.By supernatant in 95 DEG C of sterilizings
Room temperature is cooled to after 5min, its acidity is determined, pH3.71,184.53 ° of T of titratable acidity is measured.
3rd, according to centrifuged supernatant acidity after sterilizing, it is calculated every gram of NaHCO of centrifuged supernatant of theoretical neutralization3Amount is
1.55×10-2g.According to the foundation of buffering formula, 100kg fermented supernatant fluids neutralize the NaHCO required for reaching pH5.63Addition
Amount 0.93kg NaHCO3。
4th, lactobacillus acidophilus and bifidobacterium lactis are mixed according to 4: 1 ratios, total viable count is 1.4 × 1011CFU/g, meter
It is 10% that calculation obtains 0.35g tablet bacterium powders addition, and glucose addition is 15%, NaHCO3Addition is 40%, trehalose
Addition is 15%, and milk powder addition is 10%, and pearl powder addition is 5%, magnesium stearate 5%.
5th, in addition to bacterium powder, other dispensing drying and sterilizings cross 40 eye mesh screens in desinfection chamber, after being well mixed, open dehumidifier.
Operating desk after dehumidifier is mixed and compressing tablet, and fermented liquid supernatant is dispensed to spray bottle.
6th, tablet is added in skin base solution, is released rapidly without a large amount of bubbles, gas uniformly slowly discharges, without bottle explosion feelings
Condition, obtaining skin Tiny ecosystem improves preparation, and number of live bacteria of probiotics is 1 × 107CFU/g, pH value is 5.4.
Embodiment 4
1st, lactobacillus acidophilus, L. casei casei, bifidobacterium lactis, streptococcus thermophilus are cultivated in MRS respectively
Activated for two generations in base, the thalline of logarithmic phase will be grown into, in exocellular polysaccharide selectivity ESM, 37 DEG C carry out High Density Cultivation 17h,
Nutrient solution viable count respectively reaches 5.5 × 109、4×109、6×109、4.5×109CFU/g, after by the desk-top centrifugation of zymotic fluid
Machine 12,000rpm carries out centrifugation 5min, takes supernatant.
2nd, gained supernatant is mixed according to volume ratio 1: 1: 1: 1, to probiotics supernatant.Supernatant is gone out at 90 DEG C
Room temperature is cooled to after bacterium 5min, its acidity is determined, pH3.69,185.35 ° of T of titratable acidity is measured.
3rd, according to centrifuged supernatant acidity after sterilizing, it is calculated every gram of NaHCO of centrifuged supernatant of theoretical neutralization3Amount is
1.55×10-2g.According to the foundation of buffering formula, 100kg fermented supernatant fluids neutralize the NaHCO required for reaching pH5.63Addition
Amount 0.93kg NaHCO3。
4th, by lactobacillus acidophilus, L. casei casei, bifidobacterium lactis, streptococcus thermophilus according to 1: 1: 1: 1 ratio
Example mixing, total viable count is 2.9 × 1011CFU/g, it is 10%, glucose addition to be calculated 0.5g tablet bacterium powders addition
It is 25%, NaHCO3Addition is 35%, and trehalose addition is 8%, and milk powder addition is 2%, and pearl powder addition is
10%, magnesium stearate 10%.
5th, in addition to bacterium powder, other dispensing drying and sterilizings cross 40 eye mesh screens in desinfection chamber, after being well mixed, open dehumidifier.
Operating desk after dehumidifier is mixed and compressing tablet, and fermented liquid supernatant is dispensed to spray bottle.
6th, tablet is added in skin base solution, is released rapidly without a large amount of bubbles, gas uniformly slowly discharges, without bottle explosion feelings
Condition, obtaining skin Tiny ecosystem improves preparation, and number of live bacteria of probiotics is 5 × 107CFU/g, pH value is 5.2.
Skin Tiny ecosystem obtained in examples detailed above improves the stability test of preparation and active probiotic piece, by 4 series
Sample under 25 DEG C of room temperature conditions preservation 30d, in 0d, 3d, 7d, 15d, 30d, probiotics viable bacteria in sample is measured by sampling, often
Individual sample takes three Duplicate Samples, and final result is 3 average values of sample viable count.According to《GB7918.1-7918.5-1987
Cosmetics microorganism standard test method》Treatment sample, according to《GBT 4789.35-2010 microbiological test of food hygiene is eaten
Lactic acid bacteria inspection in product》Selection detection culture medium and condition of culture.Result of the test is referring to following table：
The different skin Tiny ecosystem of table 1 improves stability of the preparation at ambient temperature in preserving process
From table 1, the skin Tiny ecosystem obtained by this patent technique improves that preparation viable bacteria content is high and viable bacteria good stability.
Stability of the different activities probiotics tablets of table 2 in preserving process
From table 2, the viable bacteria content of the active probiotic piece obtained by this patent technique is high and viable bacteria good stability.
Health giving quality is tested
This research is chosen 400 adult females and is tested on the face, and its age is 25~55 years old, is divided into 4 groups, every group
100 people.This four groups skin Tiny ecosystems used correspondingly containing embodiment 1~4 improve preparation.Frequency of use is one day 2
~3 times, persistently use 4 weeks.Test observation is carried out to moisturizing level, the colour of skin uniformity and excitant respectively, and is entered by volunteer
Row questionnaire is assessed, comprehensive to obtain data.Test result is as follows.
A, moisturizing level
The test result of moisturizing level is as shown in table 3.In table 3,5 points represent that moisturizing level is very good, 4 points of expression moisture retention waters
Flat preferable, 3 points represent that moisturizing level is general, and 2 points represent that moisturizing level is poor, and 1 point represents that moisturizing level is excessively poor.
Table 3
| Product | 5 points | 4 points | 3 points | 2 points | 1 point |
| Embodiment 1 | 85 | 15 | 0 | 0 | 0 |
| Embodiment 2 | 35 | 50 | 15 | 0 | 0 |
| Embodiment 3 | 78 | 10 | 12 | 0 | 0 |
| Embodiment 4 | 32 | 48 | 20 | 0 | 0 |
From the point of view of the test result of the moisturizing level of volunteer's facial skin test, skin Tiny ecosystem improves preparation to skin
Moistening effect is obvious.
B, the colour of skin uniformity
The test result of the colour of skin uniformity is as shown in table 4.In table 4,5 points represent that the colour of skin uniformity is obviously improved, 4 points of expressions
The colour of skin uniformity makes moderate progress, 3 points of expression colour of skin uniformity indistinctions, and 2 points represent that the colour of skin uniformity has been deteriorated, 1 point of expression
The colour of skin uniformity is deteriorated.
Table 4
| Product | 5 points | 4 points | 3 points | 2 points | 1 point |
| Embodiment 1 | 10 | 53 | 27 | 0 | 0 |
| Embodiment 2 | 8 | 22 | 70 | 0 | 0 |
| Embodiment 3 | 15 | 52 | 33 | 0 | 0 |
| Embodiment 4 | 27 | 45 | 28 | 0 | 0 |
From the point of view of the test result of the colour of skin uniformity of volunteer's facial skin test, preparation is improved using skin Tiny ecosystem
The blee of volunteer have improvement, do not occur situation about being deteriorated substantially.
C, excitant
Irritating test result is as shown in table 5.In table 5,5 points of expressions are nonirritant, and 4 points indicate minimal irritation, 3
Divide and represent medium excitant, 2 points of expression intense irritations, 1 point represents strongly excitant.
Table 5
| Product | 5 points | 4 points | 3 points | 2 points | 1 point |
| Embodiment 1 | 100 | 0 | 0 | 0 | 0 |
| Embodiment 2 | 75 | 25 | 0 | 0 | 0 |
| Embodiment 3 | 84 | 16 | 0 | 0 | 0 |
| Embodiment 4 | 91 | 9 | 0 | 0 | 0 |
From the point of view of irritant experiment result, the preparation that with the addition of active probiotic composition does not have or only has to volunteer's skin
Minimal irritation.
The above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (7)
1. a kind of skin Tiny ecosystem improves preparation, it is characterised in that：It is dissolved in skin base solution by probiotics tablets and is constituted, the micro- life of the skin
State contains 1 × 10 in improving preparation6~1 × 108CFU/g active probiotics；The skin Tiny ecosystem improve the pH of preparation for 5.0~
5.6。
2. skin Tiny ecosystem as claimed in claim 1 improves preparation, it is characterised in that：The active probiotic be lactobacillus acidophilus,
One or more arbitrary proportion in L. casei casei, Lactobacillus rhamnosus, bifidobacterium longum and lactobacillus thermophilus
Mixing.
3. skin Tiny ecosystem as claimed in claim 1 improves preparation, it is characterised in that：The skin base solution exists for the active probiotic
Supernatant after fermented and cultured obtained by centrifugation.
4. skin Tiny ecosystem as claimed in claim 3 improves preparation, it is characterised in that：Containing lactic acid and extracellular many in the skin base solution
Sugar.
5. a kind of skin Tiny ecosystem as claimed in claim 1 improves the preparation method of preparation, comprises the following steps：
(1), probiotics is inoculated into exocellular polysaccharide selective medium carries out 37~40 DEG C of quiescent culture 17~24h, 12000
~18000rpm is centrifuged 3~10min, obtains probiotic powder and supernatant；It is cooled to after taking 90~95 DEG C of 3~7min of sterilizing of supernatant
Room temperature, obtains skin base solution；
(2), the titratable acidity and pH value of determination step (1) skin base solution, is calculated the NaHCO needed for neutralizing skin base solution3Amount, with
Step (1) probiotic powder, and tablet conven-tional adjuvants are mixed, and compressing tablet obtains probiotics tablets, and viable count is at least in each probiotics tablets
It is 1 × 109CFU/g。
6. preparation method as claimed in claim 5, it is characterised in that：The tablet conven-tional adjuvants include trehalose, glucose, sugarcane
Sugar, milk powder, pearl powder, Flos Rosae Rugosas pollen and magnesium stearate.
7. preparation method as claimed in claim 6, it is characterised in that：The exocellular polysaccharide selective medium is：Skimmed milk powder
5%, glucose 5%, yeast extract 0.35%, peptone 0.35%, deionized water 89.3% adjusts pH 6.2~6.4.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107753415A (en) * | 2017-11-06 | 2018-03-06 | 辽宁大学 | A kind of active probiotic facial mask and preparation method thereof |
| CN108066275A (en) * | 2018-01-30 | 2018-05-25 | 集美大学 | A kind of edible rose facial mask and preparation method thereof |
| CN108192779A (en) * | 2017-12-30 | 2018-06-22 | 高产明 | A kind of skin care scented soap and preparation method thereof |
| CN109864965A (en) * | 2019-03-28 | 2019-06-11 | 叶绿芝生物科技（中山）有限公司 | A kind of gold skin base solution composition |
| CN110025501A (en) * | 2019-05-13 | 2019-07-19 | 武汉森澜生物科技有限公司 | The composition and its preparation method and application to keep the skin wet for skin |
| CN113081945A (en) * | 2021-05-17 | 2021-07-09 | 山东君捌壹生物科技有限公司 | Probiotics extract-containing compound polyhexamethylene biguanide salt no-wash hand sanitizer and preparation method thereof |
| CN113413327A (en) * | 2021-06-21 | 2021-09-21 | 山东焦点福瑞达生物股份有限公司 | Preparation method of polysaccharide skin microecological cosmetic |
| CN114717128A (en) * | 2021-04-18 | 2022-07-08 | 青岛蔚蓝生物股份有限公司 | Lactobacillus reuteri with effects of improving aged skin and enhancing hair health and application thereof |
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| CN1468105A (en) * | 2000-10-06 | 2004-01-14 | ˹��Ʒ�˹ɷݹ�˾ | Use of probiotic lactic acid bacteria for balancing the skin's immune system |
| CN101090706A (en) * | 2004-10-04 | 2007-12-19 | 奥里尔股份有限公司 | Cosmetic or dermatologic composition against dry and/or sensitive skin |
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| US5324515A (en) * | 1991-10-22 | 1994-06-28 | Pacific Corporation | Cosmetic material obtained from a lactic acid fermentation broth |
| CN1468105A (en) * | 2000-10-06 | 2004-01-14 | ˹��Ʒ�˹ɷݹ�˾ | Use of probiotic lactic acid bacteria for balancing the skin's immune system |
| CN101090706A (en) * | 2004-10-04 | 2007-12-19 | 奥里尔股份有限公司 | Cosmetic or dermatologic composition against dry and/or sensitive skin |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107753415A (en) * | 2017-11-06 | 2018-03-06 | 辽宁大学 | A kind of active probiotic facial mask and preparation method thereof |
| CN108192779A (en) * | 2017-12-30 | 2018-06-22 | 高产明 | A kind of skin care scented soap and preparation method thereof |
| CN108066275A (en) * | 2018-01-30 | 2018-05-25 | 集美大学 | A kind of edible rose facial mask and preparation method thereof |
| CN109864965A (en) * | 2019-03-28 | 2019-06-11 | 叶绿芝生物科技（中山）有限公司 | A kind of gold skin base solution composition |
| CN110025501A (en) * | 2019-05-13 | 2019-07-19 | 武汉森澜生物科技有限公司 | The composition and its preparation method and application to keep the skin wet for skin |
| CN110025501B (en) * | 2019-05-13 | 2022-01-18 | 武汉森澜生物科技有限公司 | Composition for supplementing moisture to skin and preparation method and application thereof |
| CN114717128A (en) * | 2021-04-18 | 2022-07-08 | 青岛蔚蓝生物股份有限公司 | Lactobacillus reuteri with effects of improving aged skin and enhancing hair health and application thereof |
| WO2022222259A1 (en) * | 2021-04-18 | 2022-10-27 | 青岛蔚蓝生物股份有限公司 | Lactobacillus reuteri having improved anti-aging and hair health enhancement effects, and application thereof |
| CN114717128B (en) * | 2021-04-18 | 2023-11-17 | 青岛蔚蓝生物股份有限公司 | Lactobacillus reuteri with effects of improving aging skin and enhancing hair health and application thereof |
| CN113081945A (en) * | 2021-05-17 | 2021-07-09 | 山东君捌壹生物科技有限公司 | Probiotics extract-containing compound polyhexamethylene biguanide salt no-wash hand sanitizer and preparation method thereof |
| CN113413327A (en) * | 2021-06-21 | 2021-09-21 | 山东焦点福瑞达生物股份有限公司 | Preparation method of polysaccharide skin microecological cosmetic |
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