FR2684295A1 - NUTRIENT COMPOSITION FOR CAPILLARY USE AND PROCESS FOR PREPARING THE SAME. - Google Patents

Abstract

The nutritive composition for hair use according to the invention is a product of fermentation by an enzyme applied to a substrate containing vegetable protein and vegetable lipid. The nutritive composition for hair use, obtained by filtration of fermented products, is safe because it is composed only of harmless substances and has excellent results in preventing hair loss as well as promoting growth and regrowth hair.

Description

NUTRITIVE COMPOSITION FOR CAPILLARY USE

AND PROCESS FOR PREPARING THE SAME

  The subject of the present invention is a nutritional composition for capillary use and its method of preparation. More particularly, the invention relates to a nutritional composition for capillary use containing fermented products obtained by applying, for example, a

  mixture of lipase and proteinase or rice yeast on a substrate containing a

  vegetable protein, for example rice bran, and vegetable lipid, as well as its preparation process.

  The real mechanism of hair loss and growth is yet to be discovered.

  However, it is generally thought that the main causes of hair loss are an interruption of the blood circulation, a slow metabolism, an excess of hormones

  males, inflammation of the skin and allergies.

  A hair is composed of a part protruding above the skin and a part

  under the skin The hair bulb at the root of the hair contains a tissue called

  Papillary lules Many capillary vessels surround the hair bulb and the

  papillary blood cells are fed by the blood circulating in the capillaries,

  the renewal of cell division and hair growth.

  However, if the blood does not supply the cells enough, the cell division activity slows down, causing the hair to stop growing. As a result, it is often recognized as essential for hair growth to stimulate circulation.

  blood in the skin and activate the functions of the cells.

  The hair follicle that surrounds the hair contains an enzyme called 5-reductase that converts to 5-dihydrotestosterone by combination with the male hormone and is then transported via the blood vessels to the papillary cells, slowing down

  activity of adenylcyclase and causing a slowdown in cell division.

  Thus, the hair follicle atrophies little by little and the hair becomes fluffy, for

to finish with baldness.

  As mentioned above as examples, there are many hypotheses about the mechanism of hair loss and growth, a hypothesis of which none has been demonstrated. of products

  hair growth, claiming to contain substances that the distributors

  They think they are effective because of their understanding of the mechanism of stimulation of the blood circulation in the skin of the head and / or of the activation of papillary cells. For example, a hair composition that is really effective remains to be put

on the market.

  In such circumstances, the inventor of the present invention pursued a long

  and intense research and finally resulted in the present invention of a hair

  nutritive product which produces excellent results on the hair, and

  fermented products obtained by applying, for example, a mixture of lipase and proteinase or rice yeast on a substrate containing a vegetable protein, for example

rice, and a vegetable lipid.

  The present invention relates to a nutritional hair composition comprising

  fermented products obtained by applying, for example, a mixture of lipase and protein

  1 o se or rice yeast on a substrate containing a vegetable protein, for example bran

  of rice, and a vegetable lipid, as well as its method of preparation.

  Said substrate may contain anything that contains a vegetable protein and a lipid

  The enzyme for fermentation must have the ability to break down the protein

  This enzyme may be a lipase and a proteinaceous

  It is also possible to add other types of enzymes such as amylase.

  Fermented products undergo a filtering process and

  Nutritive capillary positions of the present invention These are harmless to the human body and show excellent results in preventing hair loss.

  and promoting their growth and regrowth.

  The present invention will now be more fully described in the description which

  follows. The substrate for the production of a nutritional hair composition according to the present invention may be all that contains a vegetable protein and a plant lipid. It may be, for example, products derived from seeds such as rice bran, sake, the

  wheat germ, fusuma bran, buckwheat millet or whole millet (soba),

  from peas such as soya beans, soy bean dregs (okara or tofu beans), soy milk, adzuki peas, soy flour or coffee grounds, or other plant products such as nuts, taro , tea flour, honey, sesame, peanuts, lotus seeds, ashitaba,

  However, beef tongues, wakame or laminar algae are particularly

  cheerful rice bran, sake dregs, fusuma bran, coffee grounds, wheat sprouts, sesame, soy cheese dregs (okara or tofu dreg), soybeans and adzuki peas they

  contain vegetable protein and vegetable lipids in well-balanced amounts.

  The enzyme used for the fermentation in the present invention must be able to break down both the vegetable protein and the vegetable liquid in said substrate. This may be a lipase and a proteinase, and others may also be added, such as for example amylase In addition to lipase and proteinase, it is also possible to use rice yeast which

  possesses the ability to produce a variety of enzymes in a living body.

  Spermato lipase is one of the lipases that can be used in the present invention.

  vention, spermato lipase which is used in the range of acidity

  yeasts, yeasts, micro-organisms, humors (biological fluids) or enzymes extracted from internal organs. Lipase of the pancreas, liver,

  tuberculosis bacteria, lipase FIB and hima lipase.

  The proteinase to be used in the present invention will preferably be acidic with the most suitable p H may be used for this purpose pepsin, trypsin or chymotrypsin,

  which have no particular active base except the amino acid residue of the

  zyme itself and do not require any particular reagents in their enzyme activities.

  We can still use most of the enzymes or cathepsins we find

  in plant enzymes and animal cells, such as papain or fici-

  can be activated by a reducing agent such as hydrocyanate, ascorbic acid or

  cysteine or glutathione. In particular, chymotryp-

  sine, trypsin, pepsin, carboxy peptidase, cathepsin A, cathepsin B, cat-

  C, cathepsin III, cathepsin IV, renal acylase 1, renal acylase 11, leukemia,

  amino peptidase, aminotripeptidase, glycilglycine dipectidase, prolitase,

  linase, plasmin, thrombin, papain, ficin, streptococcal proteinase, C 1,

  peptidase or the historyticum proteinase.

  Fermented products can be obtained by applying the enzyme to said sub-

  HPLC (High Performance Liquid Chromatography) analyzes were performed on the fermented products with the following results. These analyzes were performed using a detector (LC-9A, Shimadzu Seisakusho, Co, Ltd) and columns (3m). of

  long) Acetonitrile was used as the eluent and the temperature of the column was now

  naked at about 30 C, the flow rate being about 10 ml / min.

  The fermented products contained fatty acids such as myristic acid, palm acid and

  mitic acid, stearic acid, arachic acid, behenic acid, lignoceric acid, dodecene

  tetradecenic acid, tetradecadienic acid, pentadecenic acid, hexadecene

  oleic acid, linoleic acid, linolenic acid, eicosaenic acid, eicosedic acid,

  nicotine, arachidonic acid, eicosatrienic acid, docosenic acid and docosahexanic acid.

  The fermented products also contained amino acids such as leucine, isoleucine, lysine

  ne, methionine, cysteine, phenylalanine, tyrosine, threonine, tryptophan, valine, histidine

  arginine, aspartic acid, alanine, glutamic acid, glycine, proline and ciline.

  It is clear from the results of the analyzes that the fermented products obtained according to the present invention contain various amino acids and fatty acids. It is believed that amino acids serve to nourish the papillary cells and that the fatty acids serve to activate and dilate the capillaries, to provide moist air to the hair which gives them a

  appearance and to inhibit the 5-a-reductase contained in hair follicles.

  In order to prevent softening of the hair, it is also believed that the components taken alone or in combination stimulate the growth and regrowth of the hair. It will be appreciated that the fermented products also contain amino acids which include sulfur or unsaturated fatty acids. think they have excellent effects on growth

and hair regrowth.

  In addition to the confirmed components in said fermented products, the products obtained

  by fermentation by applying rice yeast to the substrate according to the invention containing

  small amounts of vitamin A, vitamin BI, vitamin B 2, vitamin B 6, vitamin B 12, vitamin C, vitamin E, copper, iron, calcium, phosphorus,

  purine, deoxyribonucleic acid, ribonucleic acid, adenine, guanidine,

  ne, uracil, cytosine and xanthine, obtained using a mixture of lipase and proteinase, because various enzymes, besides lipase and proteinase, are produced in the

  yeast, such as amylase, glutaminase, cellulase and pectinase,

  not only vegetable proteins and plant lipids, but also other components of the substrate. It is believed that these other components also stimulate growth and

  hair regrowth by synergy with said amino acids and fatty acids.

  To preserve the nutritional hair compositions of the present invention, it is possible to

  refrigerate or boil them However, there are no functional or

  gradation of the effects of nutritive hair compositions.

  We will give below a description of a process for the preparation of the compound

  Nutritional capillary assembly of the present invention. The process comprises the steps of

  to apply the enzyme to a substrate containing a vegetable protein and a vegetable lipid.

  and filter the fermented products thus obtained.

  The fermentation is carried out in an aqueous medium Lipase and proteinase or rice yeast which produces other enzymes such as amylase are used as an enzyme Lipase and proteinase can be applied separately or applied together in the form of a mixture. Said mixture may contain other enzymes such as amylase, so as to stimulate the decomposition function, and to decompose other components in the substrate It is advantageous to apply 0.1% by weight, relative to the substrate, of each

  enzymes in said mixture In the case of rice yeast, such as the decomposition function

  position of said yeast is small in comparison with that of lipase or proteinase, it

  It is better to add 1% by weight, relative to the substrate, of rice yeast.

  The fermentation temperature will preferably be room temperature, or

  taken at 350 C and 450 C, and it will be possible to use as a buffer solution, with a p H included

  between 6 and 11, a mixture of hydrochloric acid collidine, or potassium phosphate

  mayor secondary potassium phosphate, or hydrochloric acid soda salt from Veronal, or

  hydrochloric acid trisaminomethane, or hydrochloric acid borax, or boric acid -

  sodium carbonate, or hydrochloric acid aminoethyl propane diol, or ammonium chloride

  monium ammonia, or glycine sodium hydroxide, or boric acid hydroxide

  sodium, or hydrochloric acid dimethylglycinate sodium, or sodium bicarbonate -

  sodium carbonate, or borax sodium hydroxide, or sodium borax carbonate, or hydrochloric acid sodium carbonate, or sodium phosphate secondary sodium hydroxide, or other equivalents Any single or multiple combination of those substances

  can also be used as a mixture.

  The reaction time for the fermentation will preferably be between 5 and 10 hours, with stirring. The end of the reaction may be known, as far as the lipase is concerned, by taking as indicator a predetermined color, and by checking the quantity of acids

  extracts which are produced by hydrolysis of the ester, or by electrochemical

  If these techniques can not be easily implemented, colorimetry can be used for the alcohol extracted during the hydrolysis.

  which is proteinase, we can use the colorimetry with ninhydrin, which is the colorimetric

  sort of amino acids by the bluish color produced by ninhydrin when it is

  If this technique can not be easily implemented, it is possible to

  to use the formaldehyde assay by the method of 50 rensen This method uses the phenomenon of

  divergence of the titration curve for the α-amino or imino radical in the presence of formaldehyde

  As for sterilization, sterilization by autoclave is preferred.

  The nutritional hair composition of the present invention is obtained by adding

  water or alcohol to the fermented products after filtration of said fermented products.

  Nutritive capillary position thus obtained can be used as is or be mixed with a hair lotion, a hair cream, a capillary liquid, a hair tonic,

  an ointment, shampoo or rinse aid.

  It is possible to mix with the nutritional hair composition of the present invention

  certain components that are often used in hair treatments intended to

  promote hair growth and regrowth, such as stimulants in the circulation.

  blood, metabolic stimulants, anti-male hormones, anti-inflammatory, anti-allergenic, moisturizers, antibacterials, and / or refreshing products.

  This is, for example, tocopherol acetate, capronium chloride, carbon dioxide,

  lnokitiol, ethyl pantheryl benzoate, glyceride pentadecanate,

  vitamin H, ethinyl estradiol, glycyl-1-olicoleic acid, diphenyl hy-

  drazinium, hyaluronic acid, Minisasanishiki extract, mononitroguaiacol,

  tincture of Togarashi, Shoukyo tincture, hydrocortisone, diphenic

  hydrazinium, D-pantothenylol, salicylic acid, lactic acid, lactate

dium, menthol, etc.

Example 1

  500 g of rice bran is used as the substrate. 1000 ml of hot water is added to the rice bran (500 g) and the mixture is carefully mixed. The mixture is then placed in a container.

  such as a glass or plastic container, and 5 g of

  3 g of sodium bicarbonate are also added as a buffer to control the pH. The container is heated to a temperature of about 450.degree.

  constant keeping The mixture is left thus a whole day with occasional stirring.

  The mixture becomes pasty and this pasty liquid is filtered with a cloth filter to obtain

  about 1000 ml of a semitransparent nutritive liquid liquid.

Example 2

  200 g of soybean meal are used as the substrate. 600 ml of water are added to said soybean meal (200 g). 100 g of internal organs of animals are prepared and 200 ml of water to said internal organs of animals, and then the whole is ground with a glass homogenizer to obtain a homogenizate Said homogenate is

  then centrifuged at a speed of 1500 rpm. The supernatant fluid (100 ml) is then

  The mixture is then added to the substrate.

  The mixture is maintained at about 400 ° C. The enzyme activity is examined by means of a Kletto photo-electric colorimeter (filter NO 540) and the end. of this activity is observed after 8

hours.

  40 ml of boric acid sodium carbonate mixture (0.1 M, p H 10) is added as a buffer to the fermented liquid thus obtained. Chymotrypsin is then added to obtain a

  molarity of 0.1 M The mixture is left at about 400 C The confirmation of the end of the iron

  is obtained by measuring, with Conway's method, the amount of ammonia

  liquid samples of 0.2 ml each taken from said mixture After 10 hours

  viron, ammonia production ceases and fermentation is over Fermented liquid

  is then filtered and washed with water A nutritive hair lotion is thus obtained.

  EXAMPLE 3 200 g of Adzuki millet are used as substrate. 500 ml of water are added to this milling and the mixture is in the form of a suspension. Separately, the mixture is floated.

  lipase obtained from mycelium FIB in distilled water (100 ml), and stirred

  followed by a Knapp-March mixer for approximately 2 minutes, then leave

  at room temperature for about 20 minutes The supernatant fluid obtained after centrifugation

  fugation of the mixture at 2000 rpm for 30 minutes is added to said mixture of Adzuki milling and water. Britton-Robinson buffer (phosphoric acid, acetic acid, acid

  boric acid, caustic soda) is added to adjust the pH to 9.

  About 8 hours at about 350 ° C. The fermentation process is monitored by assay using 0.05 M alcoholic hydrochloric acid.

  Separately, commercial papain dissolved in a mixture of water and sulphuric acid

  The water is then added to the mixture for several hours. Alcohol is then added to the mixture to precipitate the papain. Water is added to the refined papain (1 g of papain, 100 ml of water). Britton-Robinson buffer to adjust the pH to 9 before adding the assembly to said fermentation liquid containing said substrate. The fermentation liquid is left at about 400 ° C. The end of the fermentation is confirmed by assay, by means of alcohol, liquid samples of 0.2 ml each taken

  on said fermentation liquid The fermentation is completed after about 12 hours.

  The fermented liquid is filtered and thus a nutritive hair lotion is obtained.

Example 4

  100 g of soy cheese lees (okara or tofu binder) are used as substrate.

  to 500 ml of water and the mixture is maintained at about 500 ° C. Separately, commercial renal lipase is dissolved (1%) in distilled water Said distilled water containing said renal lipase (5 ml) 5 ml of phosphoric acid (0.6 M, p H 7) is added as a buffer and the whole mixture is maintained at

  400 C for about 10 hours, with occasional stirring The end of fermentation

  This is confirmed by observing the cessation of carbon dioxide production in use.

reading a Warburg manometer.

  Separately, 10 g of commercial pepsin (Cuhady, 1/10 000 pepsin so-

  USP (American Pharmacopoeia) in 10 ml of a 20% ethanol solution, and the whole is added to said fermentation liquid. Ammonia hydrochloric acid mixture is also added to adjust the pH to 7. is left at about 350 C for about

  hours It is then filtered by means of a filter (5 g) The p H is then 7

  The end of the fermentation is confirmed with an apparatus of

  Conway by taking liquid samples of 0.5 ml each from the filtered liquid and

  languishing each sample with 1 ml of potassium carbonate in the same

  The end of the fermentation is known by observing the changes in the quantities of ammonia volatilizing, this by dosage The end of the fermentation is confirmed after approximately 10 hours The liquid thus obtained is filtered in the usual manner and

  gets a nutritive hair lotion.

  Evaluation of the results The nutritive capillary liquids obtained according to Examples 1 to 4 were diluted with ethanol and the mixtures were applied to men and mice daily, once in the morning and once in the evening. days of application, both types of controls showed surprising results in hair growth and regrowth.

  the case of the mice in particular, the influence on the thrust of the hair was enormous.

Claims (1)

The nutritive hair composition of Claim 1 contains fermented products containing various amino acids and fatty acids. The acids, alone or in combination, activate the circulation in the capillaries and inhibit the α-reductase contained in the hair follicle. As capillaries promote growth and regrowth of conventional hair, the nutritional hair composition of Claim 1 provides a better preventive result against hair loss and a better result on growth and regrowth of hair. Hair composition The nutrient of the present invention is not harmful to the human body because it is a fermentative and the substrate comprises vegetable proteins and plant lipids and a safe enzyme is used. With respect to the nutritive hair composition of Claim 2, the lipase and proteinase enzymes used effectively break down vegetable proteins and plant lipids used in the substrate, ensuring a high production rate of fermented products per unit of product. weight. The nutritional hair composition of Claim 3, in addition to amino acids and fatty acids, contains in small amounts vitamin E, copper, purine, nucleic acids, etc. and these elements, associated with amino acids and acids fat, enhance the action of stimulating growth and regrowth of hair. The process of Claim 4 provides a nutritional hair composition that activates the capillaries and inhibits the α-reductase that is contained in the hair follicle. The composition therefore acts to prevent hair loss and to grow and repel hair follicles. hair in a much more remarkable way than the classic hair products promoting hair growth and regrowth. The process of Claim 5 produces a high level of fermented products per unit weight because lipase and proteinase are used as enzymes that effectively ferment vegetable proteins and plant lipids. The process of Claim 6 provides a nutritional hair composition which contains in small amounts, in addition to amino acids and fatty acids, vitamin E, copper, purine, nucleic acids, etc. and these elements, associated with the acids amino and fatty acids, enhance the action of stimulating growth and regrowth of hair. The methods of Claims 7 to 9 provide for faster decomposition by means of enzymes leading to more efficient decomposition and therefore provide a better rate of production of a nutrient hair composition per unit weight. A capillary nutrient composition comprising fermentation products obtained by application of enzymes to a substrate comprising vegetable proteins and plant lipids. The capillary nutrient composition according to claim 1, wherein said enzymes are lipases and proteinases. Hair-nourishing composition according to claim 1, wherein said enzymes are enzymes produced by rice yeast. Process for the preparation of a hair nutrient composition comprising a step of applying enzymes to a substrate comprising vegetable proteins and plant lipids and a step of filtering the fermented products obtained. A process for preparing a hair nutrient composition according to Claim 4, wherein said enzymes are a mixture of lipases and proteinases. A process for preparing a hair nutrient composition according to Claim 4, wherein said enzymes are enzymes produced by rice yeast. A process for preparing a hair-care nutrient composition according to Claim 4, additionally comprising a step of adding to said substrate, as a buffer, one of, or any combination of, single or multiple H between 6 and 11 selected from the following products: hydrochloric acid collidine, potassium phosphate primary potassium phosphate secondary, hydrochloric acid soda salt Veronal, hydrochloric acid trisnamomethane, hydrochloric acid borax, boric acid sodium carbonate, hydrochloric acid aminoethyl propane diol, ammonium chloride ammonia, glycine - sodium hydroxide, boric acid sodium hydroxide, hydrochloric acid sodium dimethylglycinate, sodium bicarbonate sodium carbonate, borax sodium hydroxide, sodium borax carbonate hydrochloric acid sodium carbonate, sodium phosphate secondary sodium hydroxide, or the like. A process for preparing a hair-care nutrient composition according to Claim 5, additionally comprising a step of adding to said substrate, as a buffer, one of, or any combination of, single or multiple H between 6 and 11 selected from the following products: hydrochloric acid collidine, potassium phosphate primary potassium phosphate secondary, hydrochloric acid soda salt Veronal, hydrochloric acid trisnamomethane, hydrochloric acid borax, boric acid sodium carbonate, hydrochloric acid aminoethyl propane diol, ammonium chloride ammonia, glycine - sodium hydroxide, boric acid sodium hydroxide, hydrochloric acid sodium dimethylglycinate, sodium bicarbonate sodium carbonate, borax sodium hydroxide, sodium borax carbonate hydrochloric acid sodium carbonate, sodium phosphate secondary sodium hydroxide, or the like. A process for preparing a hair-care nutrient composition according to Claim 6, additionally comprising a step of adding to said substrate, as a buffer, one of, or any combination of, single or multiple H between 6 and 11 selected from the following products: hydrochloric acid collidine, potassium phosphate primary potassium phosphate secondary, hydrochloric acid soda salt Veronal, hydrochloric acid trisnamomethane, hydrochloric acid borax, boric acid sodium carbonate, hydrochloric acid aminoethyl propane diol, ammonium chloride ammonia, glycine -   1 sodium hydroxide, boric acid sodium hydroxide, hydrochloric acid dimethyl-   sodium glycinate, sodium bicarbonate sodium carbonate, borax hydroxide   dium, borax sodium carbonate, hydrochloric acid sodium carbonate, phosphate   secondary potassium sodium hydroxide, or the like.