CH683317A5 - A method for producing a Haarnährmittels. - Google Patents

Description

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description

Technical field

The present invention relates to a method for producing a hair nutrient. In particular, the invention relates to a process for the preparation of a hair nutrient containing fermentation products which, by the action of e.g. a mixture of lipase and proteinase or rice yeast on a substrate containing vegetable protein such as e.g. Rice bran and herbal lipids, were prepared.

State of the art

The real background for hair loss and hair growth has not yet been clarified. However, it is generally believed that the main causes of hair loss are circulatory disorders, poor metabolism, an excess of male hormones

e.g. Hydrocyanide, ascorbic acid, cysteine or glutathione can be activated. In particular, chymotrypsin, trypsin, pepsin, carboxypeptidase, cathepsin A, cathepsin B, cathepsin C ', cathepsin III, cathepsin IV, kidney acylase I, renenacylase II, leucine aminopeptidase, aminotripep tidase, glycilglycinol dipole Prolinase, plasmin, thrombin, papain, ficin, streptococcal proteinase, C1 (dostridium), histolyticum proteinase or peptidase can be used.

Fermentation products can be obtained by the action of enzyme on the above-mentioned substrate. The fermentation products were analyzed by HPLC and the following results were obtained. The above analyzes were carried out with a detector (LC-9A. Shimadzu Seisakusho, Co., Ltd.) and with columns (length 3 m). Acetonitrile solvent was used as the moving bed, the column temperature was approx. 30 ° C and the flow rate was approx. 10 ml / min.

It turned out that the fermentation products fatty acids such as contained palmitic acid, stearic acid, behenic acid, Lignoce-rinsäure, dodecenoic, tetradecenoic, nibble-cadiensäure, pentadecenoic acid, hexadecenoic, oleic acid, linoleic acid, linolenic acid, eicosaenoic acid, eicosadienoic, arachidonic acid, Eicosatriensäu-re, docosenoic and docosahexaenoic acid. It was also shown that the fermentation products contained amino acids such as leucine, isoleucine, lysine, methionine, cysteine, phenylalanine, tyrosine, threonine, tryptophan, valine, histidine, arginine, aspartic acid, alanine, glutamic acid, glycine, proline and serine.

It is clear from the results of the analyzes that the fermentation products obtained according to the present invention contain a large number of amino acids and fatty acids. It is believed that papillary cell nutrition is a function of amino acids, and the activation and expansion of capillary blood vessels, the supply of moisture to the hair to give it a shiny appearance, and the inactivation of the 5-a- Reductase in hair follicles

Stepping of a hair nutrient which has an excellent effect on the hair and which contains fermentation products which, by action, e.g. a mixture of lipase and proteinase or rice yeast on a substrate containing vegetable protein such as e.g. Rice bran and herbal lipids, were prepared.

Presentation of the invention

According to the present invention, a process for the preparation of a hair nutrient which contains fermentation products which by the action of e.g. a mixture of lipase and proteinase or rice yeast on a substrate which contains vegetable protein such as e.g. Containing rice bran and vegetable lipids can be obtained, provided.

The above substrate can be anything that contains vegetable protein and vegetable lipids. Enzymes for fermentation must contain the vegetable protein and the vegetable lipids. Ci iKetrnt enolton I / Ännün Cnl ^ ha Cn? »/ Mn

the rice yeast in addition to lipase and proteinase a variety of enzymes, such as Amylase, glutaminase, cellulase, pectinase, which not only cleave the vegetable protein and the vegetable lipids, but also other components of the substrate. It is believed that these other ingredients also help promote hair growth by synergizing with the above amino acids and fatty acids.

For preservation, the hair nutrients of the present invention can be refrigerated or boiled, but this does not result in a functional change or degradation or reduction in the effectiveness of the hair nutrients.

The process according to the invention for the production of the hair nutrient is described below. The working steps include the action of enzyme on a substrate which contains vegetable protein and vegetable lipids, and filtering the fermentation products obtained.

The fermentation is carried out under aqueous conditions. Lipase and proteinase or rice yeast, which other enzymes such as e.g. Amylase produces. Lipase and proteinase can be used separately or together, in the form of a mixture. Said mixture can contain other enzymes, e.g. Contain amylase to increase the cleavage effect and cleave other components of the substrate. A mixture of 0.1 wt.% Enzyme per 100% substrate is preferably used. In the case of rice yeast, the cleavage of which is weak compared to lipase or proteinase, it is better to use 1% by weight rice yeast per 100% substrate.

The fermentation is preferably carried out at room temperature or 35 ° C to 45 ° C, and as a buffer solution, pH 6 to 11, hydrochloric acid / collidine, primary / secondary potassium phosphate, hydrochloric acid / veronal sodium salt, hydrochloric acid / tris-aminomethane, hydrochloric acid / Borax, boric acid / sodium carbonate, hydrochloric acid / aminomethyl propanediol, ammonium chloride / ammonia, glycine / sodium hydroxide, boric acid / sodium hydroxide, hydrochloric acid / sodium dimethyl

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The lipase to be used for the present invention can be spermatolipase which is used in the acidic range and which is contained in a large number of molds, yeast, microorganisms, liquids or enzymes from internal organs. Pancreatic lipase, liver lipase, lipase from tubercle bacteria, PIB lipase and Hima lipase can be used in particular.

The proteinase to be used in the present invention is preferably acidic at the most suitable pH. In this regard, pepsin, trypsin or chymotrypsin can be used which have no particular base activity, with the exception of the amino acid residue of the enzyme itself, and which do not require a special reagent for enzymatic activity. Most of the enzymes or cathepsin that are present in plant enzymes and animal cells, such as papain or ficin, and which can be used by a reducing agent such as e.g. Hydrocyanide, ascorbic acid, cysteine or glutathione can be activated. In particular, chymotrypsin, trypsin, pepsin, carboxypeptidase, cathepsin A, cathepsin B, cathepsin C ', cathepsin III, cathepsin IV, renal acylase I, renenacylase II, leucine aminopeptidase, aminotripep tidase, glycilase prinitase, glycilglycinol dipole Prolinase, plasmin, thrombin, papain, ficin, streptococcal proteinase, C1 (dostridium), histolyticum proteinase or peptidase can be used.

Fermentation products can be obtained by the action of enzyme on the above-mentioned substrate. The fermentation products were analyzed by HPLC and the following results were obtained. The above analyzes were carried out with a detector (LC-9A. Shimadzu Seisakusho, Co., Ltd.) and with columns (length 3 m). Acetonitrile solvent was used as the moving bed, the column temperature was approx. 30 ° C and the flow rate was approx. 10 ml / min.

It turned out that the fermentation products fatty acids such as contained palmitic acid, stearic acid, behenic acid, Lignoce-rinsäure, dodecenoic, tetradecenoic, nibble-cadiensäure, pentadecenoic acid, hexadecenoic, oleic acid, linoleic acid, linolenic acid, eicosaenoic acid, eicosadienoic, arachidonic acid, Eicosatriensäu-re, docosenoic and docosahexaenoic acid. It was also shown that the fermentation products contained amino acids such as leucine, isoleucine, lysine, methionine, cysteine, phenylalanine, tyrosine, threonine, tryptophan, valine, histidine, arginine, aspartic acid, alanine, glutamic acid, glycine, proline and serine.

It is clear from the results of the analyzes that the fermentation products obtained according to the present invention contain a large number of amino acids and fatty acids. It is believed that the nutrition of the papillary cells is a function of the amino acids, and the activation and expansion of capillary blood vessels, the hydration of the hair to give it a shiny appearance, and the inactivation of the 5-a- Reductase in the hair follicle, in order to avoid hair weakening, is a function of the fatty acids. It is believed that the individual components, or a combination of the components, additionally promote hair growth. It should be noted here that the fermentation products also contain amino acids with sulfur and unsaturated fatty acids, and these substances are believed to have an excellent effect on hair growth.

It was found that the fermentation products obtained by the action of rice yeast on the substrates according to the present invention, in addition to the components which were confirmed in the above-mentioned fermentation products obtained by a mixture of lipase and proteinase, small amounts of vitamin A, Contains vitamin Bi, vitamin B2, vitamin B6, vitamin B12, vitamin C, vitamin E, copper, iron, calcium, phosphate, purine, deoxyribonucleic acid, ribonucleic acid, adenine, guanine, uracil, cytosine and xanthine, since the rice yeast in addition to lipase and proteinase a variety of enzymes, such as Amylase, glutaminase, cellulase, pectinase, which not only cleave the vegetable protein and the vegetable lipids, but also other components of the substrate. It is believed that these other ingredients also help promote hair growth by synergizing with the above amino acids and fatty acids.

For preservation, the hair nutrients of the present invention can be refrigerated or boiled, but this does not result in a functional change or degradation or reduction in the effectiveness of the hair nutrients.

The process according to the invention for the production of the hair nutrient is described below. The working steps include the action of enzyme on a substrate which contains vegetable protein and vegetable lipids, and filtering the fermentation products obtained.

The fermentation is carried out under aqueous conditions. Lipase and proteinase or rice yeast, which other enzymes such as e.g. Amylase produces. Lipase and proteinase can be used separately or together, in the form of a mixture. Said mixture can contain other enzymes, e.g. Contain amylase to increase the cleavage effect and cleave other components of the substrate. A mixture of 0.1 wt.% Enzyme per 100% substrate is preferably used. In the case of rice yeast, the cleavage of which is weak compared to lipase or proteinase, it is better to use 1% by weight rice yeast per 100% substrate.

The fermentation is preferably carried out at room temperature or 35 ° C to 45 ° C, and as a buffer solution, pH 6 to 11, hydrochloric acid / collidine, primary / secondary potassium phosphate, hydrochloric acid / veronal sodium salt, hydrochloric acid / tris-aminomethane, hydrochloric acid / Borax, boric acid / sodium carbonate, hydrochloric acid / aminomethyl propanediol, ammonium chloride / ammonia, glycine / sodium hydroxide, boric acid / sodium hydroxide, hydrochloric acid / sodium dimethyl

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thylglycinate, sodium bicarbonate / sodium carbonate, borax / sodium hydroxide, borax / sodium carbonate, hydrochloric acid / sodium carbonate, secondary sodium phosphate / sodium hydroxide or similar be used. Any combination or all combinations of the abovementioned substances can also be used in the form of a mixture.

The reaction time of the fermentation is preferably 5 to 10 hours with stirring. As in the case of lipase, the end of the reaction can be determined by adding a predetermined dye as an indicator and determining the amount of fatty acids released which are obtained by ester hydrolysis or by electrical titration with alkali. If this procedure proves to be difficult, the alcohol released by hydrolysis must be determined colorimetrically. As in the case of proteinase, ninhydrin colorimetry, i.e. Use colorimetry of amino acids through the bluish substance that arises when ninhydrin is heated. If this is difficult to carry out, S0rensen form titration can be used. This method takes advantage of the fact that the titration curve deviates for an a-amino residue or imino residue in the presence of formaldehyde. An autoclave is preferably used for sterilization.

A hair nutrient according to the present invention is obtained by filtering these fermentation products and adding water or alcohol. The hair nutrient obtained can be used in this state or hair lotions, hair creams, hair lotions, hair tonics, pomades, shampoos or hair conditioners can be added.

Ingredients which are frequently used in conventional hair restorants, such as e.g. blood circulation-promoting substances, metabolism-promoting substances, antagonists to male hormones, anti-inflammatory substances, anti-allergenic substances, moisturizing substances, antibacterial substances and / or cooling agents are added. These are e.g. around tocophero-acetate, capronium chloride, carbonic acid gas, hinokiti-ol, pantonyl ethyl ether benzoinate, glyceride pentade canate, vitamin H, ethynyl estradiol, glythylricinic acid, diphenylhydramine chloride, hyaluronic acid, Minisa sanishiki extract. Mononitroguaiacol, Toaarashi tincture, Shoukvo tincture. Hydrocortisone, diphenichyamine chloride, D-pantothenyl alcohol, salicylic acid, lactic acid, sodium lactate, menthol etc.

Examples

Example 1:

Rice bran was used as the substrate. Warm water (1000 ml) was added to the above-mentioned rice bran (500 g) and everything was mixed well. The mixture was then transferred to a suitable vessel, e.g. a glass or plastic jar and rice yeast (5 g) was added to the above mixture. Sodium bicarbonate (8 g) was added to control the pH. The vessel was heated to about 45 ° C by means of a heater and then kept at this temperature. With occasional stirring, the mixture was left to stand for a whole day. The mixture became dough-like in consistency, and this dough-like liquid in the jar was filtered through a cloth so as to obtain about 1000 ml of a semi-transparent liquid hair nutrient.

Example 2:

Soybean sediment was used as the substrate. Water (600 ml) was added to the above-mentioned soybean soil set (200 g). Animal offal (100 g) was prepared and water (200 ml) was added to the above animal offal, and this mixture was then ground in a glass homogenizer to prepare a homogenate. This homogenate was centrifuged at 1500 rpm. The supernatant (100 ml) was then drawn off and added to the substrate. At the same time, sodium carbonate / boric acid was added as a buffer to adjust the pH to 10.0. The mixture was kept at a temperature of about 40 ° C. The enzyme activities were monitored by means of a photoelectric colorimeter (Kletto) (filter No. 540), and an end of the activity was found after approximately 8 hours.

0.1 M (pH 10.0) sodium carbonate / boric acid was added in an amount of 40 ml as a buffer to the fermentation broth thus obtained. 0.1 M chymotrypsin was then added. This mixture was kept at a temperature of about 40 ° C. The end of fermentation was measured by measuring the amount of ammonium in the 0.2 ml of liquid sampled from the above mixture. The ammonium evolution ended after about 10 hours, which meant the end of the fermentation. The fermentation broth was then filtered and washed with water. A liquid hair nutrient was thus obtained.

Example 3:

Adzuki sediment was used as substrate. Water (500 ml) was added to the above Adzuki sediment (200 g) and a suspension was prepared. Separately, lipase obtained from FIB-Mycelium was poured onto 100 ml of distilled water, and this was then stirred for about 2 minutes with a Knapp-March mixer and left to stand at room temperature for about 20 minutes. The mixture was centrifuged at 2000 rpm for 30 minutes and the top layer was added to the above mixture of Adzuki sediment and water. The pH was adjusted to 9.0 by adding Britton-Robinson buffer (phosphoric acid, acetic acid, boric acid, caustic soda). The mixture was kept under shaking at a temperature of about 35 ° C. for about 8 hours. The fermentation process was followed by titration with 0.05 M alcoholic hydrochloric acid.

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Separately, commercially available papain was dissolved in water and hydrogen sulfide was bubbled through the mixture for a few hours. Alcohol was then added to the above mixture to precipitate the papain. Water was added to the purified papain (1 g papain: 100 ml water) and the pH was then adjusted to 9.0 by adding Britton-Robinson buffer, and this liquid was added to the above-mentioned fermentation broth including substrate. This fermentation broth was kept at a temperature of about 40 ° C. The end of the fermentation was confirmed by alcohol titration of 0.2 ml samples taken from the above fermentation broth. The fermentation was complete after about 12 hours. The fermentation broth was filtered to give a liquid hair nutrient.

Example 4:

Tofu waste was used as the substrate. Water (500 ml) was added to the above tofu waste (100 g) and the mixture was kept at a temperature of about 50 ° C. Commercially available kidney lipase (1%) was separately dissolved in distilled water. This distilled water containing the above kidney lipase (5 ml) was added to the above mixture. Phosphoric acid (0.6 M, pH 7.0, 5 ml) was also added as a buffer and the mixture was kept at a temperature of about 40 ° C for about 10 hours with occasional shaking. The end of the fermentation was confirmed by determining the end of the carbon dioxide gas evolution using a Warburg manometer.

Commercially available pepsin (Cudahy, 1/10000 ELP, soluble pepsin; 10 g) was separately dissolved in 10 ml of a 20% ethanol solution and this solution was added to the above fermentation broth. Hydrochloric acid / ammonium was also added to bring the pH to 7.5. This liquid was kept at a temperature of about 35 ° C for about 20 hours. It was then filtered through a filter cell (5 g filter substance). During this process the liquid had a pH of 7.0. The filtrate was left at about 37 ° G. The end of the fermentation was confirmed by mixing 0.5 ml samples, which had been taken from the filtrate outside of a Conway apparatus, with 1 ml of potassium carbonate, with which the above-mentioned apparatus had been charged. The end of the fermentation was demonstrated by titration to track the changes in the amount of ammonia released.

The end was confirmed after about 10 hours. The broth obtained was filtered in the usual way and a liquid hair nutrient was obtained.

Impact assessment

The liquid hair nutrients obtained according to Examples 1 to 4 were diluted with ethanol, and the mixtures were applied once a day in the morning and once in the evening to humans and mice. After thirty days of use, an excellent effect on hair growth was shown in both cases. The hair growth effect was extremely pronounced in the mice.

The process for the preparation of the hair nutrient according to claim 1 contains fermentation products which contain a large number of amino acids and fatty acids. Individually or in combination, the acids activate capillary blood vessels and inactivate the 5-a reductase, which is contained in the hair follicle. In comparison with conventional hair growth agents, the hair nutrient produced according to claim 1 shows a much better preventive effect against hair loss as well as a much more pronounced hair growth effect. The hair nutrient according to the present invention is completely harmless to humans since it is a fermentation product, the substrate consists of vegetable protein and vegetable lipids, and a harmless enzyme is used.

As for the method for producing the hair nutrient according to claim 3, lipase and proteinase used as the enzyme cleave the vegetable protein and the vegetable lipids used in the substrate in an effective manner, which necessitates a high production rate of fermentation products per unit weight.

The method for producing the hair nutrient according to claim 4 contains, in addition to amino acids and fatty acids, small amounts of vitamin E, copper, purine, nucleic acid, etc., which together with the amino acids and the fatty acids further promote hair growth.

The method according to claim 2 provides a hair nutrient according to the present invention which activates capillary vessels and inactivates the 5-a reductase in the hair follicle. The remedy therefore works against hair loss and allows hair to grow better than normal hair restoration products.

The method according to claim 3 provides large amounts of fermentation products per unit weight, since lipase and proteinase are used as enzymes, which ferment the vegetable protein and the vegetable lipids in the substrate with a high conversion rate.

The method according to claim 4 provides a hair nutrient which, in addition to amino acids and fatty acids, contains small amounts of vitamin E, copper, purine, nucleic acids etc. which, together with the amino acids and the fatty acids, further promote hair growth.

Claims 5 to 7 provide methods for faster enzymatic cleavage, which leads to better cleavage rates and therefore to better production rates of hair nutrients per unit weight.

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Claims (7)

Claims 1. A process for the preparation of a hair nutrient containing fermentation products, the fermentation products being obtained by the action of enzymes on a substrate which contains vegetable protein and vegetable lipids. 2. A process for the preparation of a hair nutrient according to claim 1 with a process step in which the fermentation products obtained are filtered. 3. A method for producing a hair nutrient according to claim 1 or 2, wherein the above-mentioned enzymes are a mixture of lipase and proteinase. 4. A method for producing a hair nutrient according to claim 1 or 2, wherein the above-mentioned enzymes are produced from rice yeast. 5. A process for the preparation of a hair nutrient according to claim 2, which additionally comprises a process step in which the above-mentioned substrate is a buffer from one or any combination or all combinations of the chemicals with pH 6 to 11 from a group which, for example, comprises contains the following substances, is added: Saic acid / collidine, primary / secondary potassium phosphate, hydrochloric acid / veronal sodium salt, saic acid / trisaminomethane, hydrochloric acid / borax, boric acid / sodium carbonate, hydrochloric acid / aminomethyl propanediol, ammonium chloride / ammonia, glycine / sodium hydroxide, boric acid / sodium hydroxide, hydrochloric acid / Sodium dimethylglycinate, sodium bicarbonate / sodium carbonate, borax / sodium hydroxide, borax / sodium carbonate, hydrochloric acid / sodium carbonate, secondary potassium phosphate / sodium hydroxide. 6. A process for the preparation of a hair nutrient according to claim 3, which additionally comprises a process step in which the abovementioned substrate is a buffer from one or any combination or all combinations of the chemicals with a pH value of 6 to 11 from a group which, for example, comprises the contains the following substances, is added: Saic acid / collidine, primary / secondary potassium phosphate, hydrochloric acid / veronal sodium salt, hydrochloric acid / trisaminomethane, hydrochloric acid / borax, boric acid / sodium carbonate, hydrochloric acid / aminomethyl propanediol, ammonium chloride / ammonia, glycine / sodium hydroxide, boric acid / sodium hydroxide, hydrochloric acid / Sodium dimethylglycinate, sodium bicarbonate / sodium carbonate, borax / sodium hydroxide, borax / sodium carbonate, hydrochloric acid / sodium carbonate, secondary potassium phosphate / sodium hydroxide. 7. A process for the preparation of a hair nutrient according to claim 4, which additionally comprises a process step in which the above-mentioned substrate is a buffer from one or any combination or all combinations of the chemicals with a pH of 6 to 11 from a group which, for example contains the following substances: Saic acid / collidine, primary / secondary potassium phosphate, hydrochloric acid / veronal sodium salt, hydrochloric acid / trisaminomethane, hydrochloric acid / borax, boric acid / sodium carbonate, hydrochloric acid / aminomethylpropane diol, ammonium chloride / ammonia, glycine / sodium hydroxide, boric acid / sodium hydroxide, hydrochloric acid / sodium di -methylglycinate, sodium bicarbonate / sodium carbonate, borax / sodium hydroxide, borax / sodium carbonate, hydrochloric acid / sodium carbonate, secondary potassium phosphate / sodium hydroxide. 5 10th 15 20th 25th 30th 35 40 45 50 55 60 65 6