KR20190073289A - Cosmetic Composition comprising Extract of Agaricus blazei - Google Patents

Abstract

The present invention relates to a cosmetic composition for improving skin condition comprising an extract of White mushroom as an active ingredient, having an effect of improving skin whitening, wrinkles and skin elasticity.

Description

The present invention relates to a cosmetic composition containing an extract of Mushroom mushroom as an active ingredient (Cosmetic Composition Containing Extract of Agaricus blazei)

The present invention relates to a cosmetic composition containing, as an active ingredient, a white mushroom extract having whitening, skin elasticity and wrinkle-reducing effects.

Aging is largely divided into natural aging or endogenous aging and external aging. Natural aging is due to genetic causes, so it is difficult to control. Therefore, studies have been carried out to prevent external aging, and in particular, research on prevention of wrinkle formation due to extrinsic photoaging by long-term ultraviolet exposure has been attracting attention (Gilchrest BA, J. Am. Acad. Dermatol., 1989: 21: 610-613). The clinical features of the exogenous skin aging are aging skin, loss of elasticity, irregular pigmentation, and deep wrinkles.

The active ingredients for skin wrinkle improvement or anti-aging currently being developed can not be used as a cosmetic raw material or are very unstable, and it is not easy to deliver to the skin, so a special stabilization system and delivery system are required. And is not visible. Recently, attention is increasingly focused on retinoid-containing skin protectants. The retinoid has been used as a means for solving photoaging phenomena such as wrinkles, skin thickening, sagging and reduction in elasticity, which are accumulated as daylight. However, since retinoids are very unstable compounds, they are sensitive to ultraviolet rays, moisture, heat, and oxygen and cause chemical changes easily. Therefore, researches have been concentrated on the development of effective ingredients derived from natural products to solve these problems.

The inventors of the present invention have studied to find substances derived from natural materials that can improve the skin condition. As a result, it has been found that the mushroom extract of the white mushroom has an effect of inhibiting the activity of elastase, tyrosinase and collagenase The present invention has been completed.

1. Korean Patent No. 10-0535875

It is an object of the present invention to provide a cosmetic composition having an effect of improving wrinkles, skin whitening or skin elasticity.

In order to achieve the above object, one aspect of the present invention provides a cosmetic composition comprising Agaricus blazei extract as an active ingredient.

The white mushroom belongs to the mushroom, the wormwood mushroom, the neck wrinkle mushroom, and is also called Agaricus mushroom and the spore mushroom. It is rich in various nutrients such as proteins, fats, fibers, vitamins, inorganic salts and nucleic acids, and is known to be effective in various diseases including cancer.

According to one embodiment of the present invention, the mushroom extract may be prepared according to a conventional method known in the art. Specifically, after washing, drying and pulverizing the white mushroom, the mushroom extract can be prepared using a conventional solvent under the conditions of ordinary temperature and pressure.

The solvent may be selected from the group consisting of distilled water, alcohols having 1 to 4 carbon atoms, ethyl acetate, chloroform, diethyl ether, dichloromethane, hexane, acetone, and mixtures thereof, preferably distilled water.

According to one embodiment of the present invention, there is provided a cosmetic composition comprising an extract of Agaricus blazei as an active ingredient,

The white mushroom extract,

Washing and drying the white mushroom;

The dried white mushroom was put into an extraction tank, and purified water having a weight of 8 to 10 times the total weight of white mushroom was added, followed by extraction at 110 to 125 ° C for 5 to 9 hours. The first extract was recovered from the extraction tank ;

After addition of purified water having a weight of 8 to 10 times the total weight of the white mushroom remaining in the extraction tank in which the first extract was collected, the extract was extracted at 110 to 125 캜 for 5 to 9 hours, and the second extract ; And

Mixing the recovered first extract and the second extract and filtering them with a filter.

According to one embodiment of the present invention, the white mushroom extract may be one obtained by an extraction method which additionally performs a step of filtering with the filter and then concentrating the filtered extract in vacuum.

According to one embodiment of the present invention, the step of concentrating the vacuum may be to concentrate to a concentration of 5 brix or more.

On the other hand, the mushroom extract includes not only the extract obtained by the solvent extraction method described above but also the extract obtained through ordinary purification process. For example, a fraction obtained through various purification methods such as separation using an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity or affinity) May also be included in the peel extract of the present invention. In addition, the mushroom extract may be prepared in powder form by an additional process such as vacuum distillation and freeze-drying or spray drying. Specifically, the mushroom extract may be prepared in powder form through sterilization and spray drying, sterilized at a temperature of 70 to 90 ° C for 10 to 40 minutes, and then powdered using a spray dryer have.

According to one embodiment of the present invention, the first extract and the second extract are those obtained by performing extraction at 110 to 125 캜, 115 to 125 캜, 115 to 121 캜, 118 to 121 캜, or 120 to 121 캜 When extracting at a temperature lower than 110 캜, active ingredients having wrinkle improvement, skin whitening, skin elasticity enhancement, skin irritation mitigation, or moisturizing effect may not be sufficiently extracted, and extraction at a temperature exceeding 125 캜 The active ingredient may be denatured.

According to one embodiment of the invention, the first extract and the second extract are extracted for 5 to 9 hours, 6 to 9 hours, 5 to 8 hours, 6 to 8 hours, 7 to 8 hours, or 6 to 7 hours , And when the extraction is performed for less than 5 hours, the active ingredient having wrinkle improvement, skin whitening, skin elasticity enhancement, skin irritation mitigation, or moisturizing effect may not be sufficiently extracted, and 9 hours The components extracted beforehand may be denatured to reduce wrinkles, skin whitening, skin elasticity enhancement, skin irritation mitigation, or moisturizing effect according to one embodiment of the present invention.

According to one embodiment of the present invention, the first extract and the second extract may be those obtained by performing the extraction under the high pressure condition, and the atmospheric pressure in the extraction tank in which the extraction is performed may be 1 to 10 atm, 1 to 8 atm, 1 to 5 atm, 1.1 to 5 atm, 1.1 to 4 atm, 1.1 to 3 atm, 1.1 to 2.1 atm, 1.1 to 1.5 atm, 1.1 to 1.2 atm, or 1.2 to 1.5 atm.

According to one embodiment of the present invention, the filter may have a pore size of 0.5 to 2.0 占 퐉, 0.5 to 1.5 占 퐉, 0.5 to 1.0 占 퐉, or 1 占 퐉.

According to one embodiment of the present invention, the cosmetic composition may inhibit the activity of at least one enzyme selected from the group consisting of elastase, tyrosinase, and collagenase.

According to one embodiment of the present invention, the cosmetic composition may have one or more effects selected from the group consisting of wrinkle improvement, skin whitening, skin elasticity enhancement, skin irritation mitigation, and moisturizing effect.

In the present invention, "whitening" means whitening the skin and means all actions that inhibit the synthesis of melanin and inhibit or prevent skin deposition of melanin.

According to one embodiment of the present invention, the composition may be one that inhibits the activity of tyrosinase.

In the present invention, " tyrosinase " is a metal-containing protein containing a pair of copper ions in its active site, which undergoes a hydroxylation reaction of L-tyrosine in the pathway of melanin formation to form 3,4- dihydroxyphenylalanine L-DOPA), which is then oxidized again with phenylalanine-3,4-quinone (dopaquinone) and then synthesized into melanin via various intermediates such as dopachrome, indole-5,6- Quot; enzyme "

Since the mushroom extract according to one embodiment of the present invention has a tyrosinase inhibitory activity in a concentration-dependent manner, the composition according to one embodiment of the present invention may have a whitening activity.

In the present invention, "elastase" means an enzyme having a role of catalyzing the hydrolysis reaction of elastin involved in skin elasticity.

In one embodiment of the present invention, the mildew extract has an elastase inhibitory activity in a concentration-dependent manner, so that the composition according to one embodiment of the present invention may have wrinkle-improving activity.

The composition according to one embodiment of the present invention may be to inhibit collagen fiber damage.

Collagen fiber is a fibrous tissue composed of collagen and exists in connective tissue such as bone, cartilage, skin, tendon. When the skin is exposed to ultraviolet rays, collagen fibers involved in skin elasticity are damaged, and the skin ages.

In one embodiment of the present invention, the mushroom extract of the present invention has collagen fiber damage-inhibiting activity in a concentration-dependent manner, so that the composition according to one embodiment of the present invention may have wrinkle-improving activity.

The cosmetic composition of the present invention may contain, as an active ingredient, the ingredients commonly used in cosmetic compositions besides the above-mentioned mushroom extract, such as conventional adjuvants such as stabilizers, solubilizers, vitamins, pigments and fragrances, and Carrier.

Meanwhile, the cosmetic composition of the present invention may be prepared in any form conventionally produced in the art, and examples thereof include a solution, a suspension, an emulsion, a paste, a gel, a cream, a lotion, a powder, a soap, Containing cleansing oil, powdered foundation, emulsion foundation, wax foundation, and spray, but is not limited thereto.

According to one embodiment of the present invention, the cosmetic composition may be in the form of a soft lotion, a gel, a water soluble liquid, a milk lotion, a cream, an essence, a skin toner, an oil-in-water emulsion, a water- Emulsion, cleansing foam, cleansing water, pack, body oil, body lotion, underwater type make-up base, in-water type make-up base, and foundation.

When the formulation of the cosmetic composition of the present invention is a paste, a cream or a gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide Can be used.

When the formulation of the cosmetic composition of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, Propane / butane, or dimethyl ether. ≪ RTI ID = 0.0 >

When the formulation of the cosmetic composition of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate , Propylene glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid esters of sorbitan can be used.

When the formulation of the cosmetic composition of the present invention is in the form of a suspension, the carrier component may include water, a liquid diluent such as ethanol or propylene glycol, ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester Suspending agent, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the cosmetic composition of the present invention is a cleansing agent containing an interfacial active agent, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, Fatty acid amide ether sulfate, alkylamido betaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester.

The mushroom extract according to one embodiment of the present invention is excellent in the activity of inhibiting the activity of elastase, tyrosinase and collagenase, and thus can be effectively used as an effective ingredient of a cosmetic composition for skin improvement.

1A to 1C show the extraction yields of the mushroom extract according to the change of extraction conditions. FIG. 1A shows the extraction yield according to the extraction temperature (60, 80, 90, 100, or 120 DEG C) Shows the extraction yield according to the extraction time (6, 8, 10 or 12 hours) and FIG. 1c shows the extraction yield according to the ethanol content (0, 30, 50 or 70%).
FIG. 2 is a schematic view illustrating a process for preparing white mushroom extract according to one embodiment of the present invention.
Fig. 3 shows the results of measurement of the inhibitory activity of elastase of white mushroom extract.
Fig. 4 shows the results of measuring collagenase inhibitory activity of the mushroom extract.
Fig. 5 shows the amount of MMP-1 expressed in human skin fibroblasts when treated with the mushroom extract.
6 shows the expression amount of type I collagen measured in treating human mussel extract with human skin fibroblasts.
Fig. 7 shows the result of measuring the tyrosinase inhibitory activity of the mushroom extract.

Hereinafter, one or more embodiments will be described in more detail by way of examples. However, these embodiments are intended to illustrate one or more embodiments and the scope of the present invention is not limited to these embodiments.

Example 1: Preparation of white mushroom extract

Example 1-1. Establishment of extraction conditions

In order to confirm the extraction yield according to the extraction conditions, the following experiment was conducted.

The white mushroom ( Agaricus blazei ) was purchased from Kim Sang Min Agaricus (Jeju, Korea), and the adhered material was removed, washed with clean fresh water, and completely dried at room temperature. Dried white mushroom was added to the extraction tank and hot water or ethanol was added at a weight of 8 to 10 times the weight of white mushroom to extract a total of 2 times. At this time, the extraction temperature was changed to 60 ° C, 80 ° C, 90 ° C, 100 ° C and 120 ° C, and the extraction time was changed to 6 hours, 8 hours, 10 hours and 12 hours. Ethanol was 0% (hot water), 30%, 50% and 70%.

As a result, it was confirmed that the extraction yield was increased as the extraction temperature was increased as shown in FIG. Also, as shown in FIG. 1B, when the extraction time was changed only at the same extraction temperature (120 ° C), the extraction yield was constant within the error range regardless of the extraction time.

When ethanol was extracted twice at 70 ° C for 6 hours using ethanol as an extraction solvent, the extraction yield was found to increase with increasing ethanol concentration as shown in FIG. 1c. The extraction yield was 51.4% for the control group (hot water extraction) extracted twice at 120 ° C for 8 hours. However, unlike the hot - water extract, the ethanol extracts showed insoluble components as they were concentrated at a high concentration. When the ethanol extracts were not concentrated, the insoluble components precipitated as the ethanol evaporated.

In addition, the use of ethanol extract as a raw material for foods or cosmetics may affect the formulation of the final product. Therefore, hot water was used as an extraction solvent in the subsequent experiments.

Examples 1-2. Production of white mushroom extract

Dried white mushroom was added to the extraction tank and purified water was added at a weight of 8 to 10 times the weight of white mushroom. At 121 DEG C and 1.2 to 1.5 atm for 6 hours to 8 hours to recover the primary extract and further add purified water of 8 to 10 times the weight of white mushroom to the extraction tank. Under the conditions of 121 占 폚 and 1.2 to 1.5 atm for 6 hours to 8 hours to recover a secondary extract. The collected primary extract and secondary extract were mixed and filtered through a filter having a pore size of 1.0 탆 (Rainbow, Korea) to obtain a hot-water extract (hereinafter, referred to as Extract 1) extracted at 121 캜.

The filtered extract was concentrated in vacuo above 5 brix. After extracting the concentrate of white mushroom extract, it was confirmed that it was safe by examining the presence or absence of foreign body, foreign body, moisture, common bacteria and E. coli.

The method for producing the mushroom extract according to this embodiment is shown in Fig. 2 in order. Except that the temperature was changed to 60 占 폚 (hereinafter referred to as Extract 2), 80 占 폚 (hereinafter referred to as Extract 3), 90 占 폚 (hereinafter referred to as Extract 4) Mushroom extract 2 to extract 5 were prepared.

Example 2. Analysis of Phenol Content of White Mushroom Extracts

The content of phenol in the mushroom extract 1 prepared in Example 1-2 was analyzed.

10 ml of third-order distilled water was added to 1 ml of hot water extract of mushroom (Extract 1) extracted at 121 ° C, and 2 ml of Folin-Ciocalteu phenol solution was added and mixed. After reacting at room temperature for 5 minutes, 2 ml of 20% sodium carbonate solution was added, and further reaction was carried out at room temperature for 2 hours. When a mixture of blue color was generated, the absorbance was measured at 680 nm and the quantitative curve was confirmed using catechin, an indicator material. The results showed that the content of phenol in 1 g of white mushroom extract was expressed in ㎎ units.

As a result, as shown in Table 1, the total phenol content of the white mushroom extract was 898.96 mg / g.

extract Total phenol content (mg / g) White mushroom extract 1 898.96 + - 42.66

Example 3 Inhibitory Effect on Elastase Activity

The dermal tissue of the skin forms a network structure of collagen and elastin, and when the elastin is decomposed by elastase, the network structure of the skin is disassembled and the skin is wrinkled. Therefore, in order to confirm the skin improving effect of the mushroom extract, the inhibition of elastase activity was examined.

Elastase hydrolyzes N-succinyl-Ala-Ala-Ala-p-nitroanilide to produce p-nitroaniline . 100 μl of a 2 mM N-Succinyl-Ala-Ala-p-nitroanilide solution was prepared using 0.1 M Tris-Cl buffer (pH 8.0), and to this solution was added retinol (positive control) 2 was added at a concentration of 100 μg / ml and mixed lightly. 50 μl of Elastase solution (0.1360 units / ml, E7885, SIGMA USA) was added, and reacted at 37 ° C for 10 minutes. Absorbance was measured at 410 nm (Synergy2, BioTek Inc., USA). The inhibition rate of the elastase activity was calculated according to the following formula 1, and is shown in Table 2 and FIG.

As a result, as shown in the following Table 2 and FIG. 3, it was confirmed that the inhibitory activity of the mildew extract 1 was about 25% higher than that of the positive control retinol.

extract Elastase inhibitory activity (%) Retinol (positive control) 19.26 ± 1.00 White mushroom extract 1 34.35 + - 0.38

Example 4. Collagenase inhibitory activity

Collagen is the main protein of all connective tissues such as skin, blood vessels and muscles, which accounts for 70% to 80% of skin dryness and is degraded by collagenase. In this test, the collagenase inhibitory activity of a sample containing mushroom extract was first screened using 4-Phenylazobenzyloxycarbonyl-Pro-Leu-Gly-Pro-D-Arg trifluoroacetate salt, which is used as a substrate of collagenase Respectively.

Proliferating cell lines were prepared by adding 4 mM CaCl ₂ to 0.1 M Tris-HCl buffer (pH 7.5) and dissolving 4-phenyl azobenzyloxycarbonyl-Pro-Leu-Gly-Pro-D-Arg (Sigma, USA) (Sigma, USA) solution at a concentration of 0.2 mg / ml, and the mushroom extract 1 (100 占 퐂 / ml) prepared in Example 1-2 were added, and the mixture was allowed to stand at room temperature for 20 minutes . As a positive control, ascorbic acid (100 μg / ml) was used. Then, the reaction was stopped by adding 250 cit of a citric acid solution of 6%, and 1.5 ml of ethyl acetate was added thereto, and the absorbance at 320 nm was measured. The collagenase inhibitory activity (%) was calculated according to the following formula (2) and shown in Table 3 and FIG.

As a result, as shown in Table 3 and FIG. 4, it was found that the collagenase inhibitory activity of the white mushroom extract was about twice as good as that of ascorbic acid, which is a positive control group.

extract Collagenase inhibitory activity (%) Ascorbic acid (positive control) 5.49 ± 2.14 White mushroom extract 1 11.88 ± 1.69

Example 5. Evaluation of MMP-1 Inhibitory Activity

The collagenase, MMP-1 (Matrix metalloproteinase-1), is one of the most effective enzymes in skin collagen decline. The active ingredient inhibiting MMP-1 can inhibit aging of the skin, It has a good effect on improving elasticity and preventing skin aging. The collagenase inhibitory activity of Example 4 was tested for specific substrates defined in vitro. In Example 5, however, by confirming the inhibitory activity of MMP-1 produced in human dendritic fibroblasts (HDF) (ADME), which analyzes the ADME (Absorption, Distribution, Metabolism, Excitation) of a sample to a cell, Wrinkle improving activity.

The amount of RNA expression of MMP-1 was measured using qRT-PCR to determine whether the composition containing the mushroom extract according to one embodiment of the present invention inhibits MMP-1 expression to inhibit MMP-1 I looked at it. 10 ng / ml of proinflammatory cytokine TNF-α and 50 μg of the mushroom extract 1 prepared in Example 1-2 for 12 to 24 hours according to the manufacturer's instructions using TRIzol reagent (Life Technologies, CA, USA) / ㎖, 100㎍ / ㎖ or 200㎍ / human skin fibroblasts in a concentration of ㎖ the HDF cells were incubated at (human dermal fibroblast, ATCC than HDF cells, ATCC) 37 ℃, CO ₂ conditions for 24 hours after the treatment on The total RNA was extracted.

Mu] l of M-MLV RT (Promega, Madison, WI), 5 [mu] l of buffer, 3 [mu] l of 10 mM dNTPs, 0.45 [ WI, USA) and 3.75 [mu] l of 20 [mu] M oligo dT (Bioneer, Daejeon, Korea) were used to reverse transcribe 5 쨉 g of total RNA.

2 μl of a reaction mixture consisting of 10 μl of RNaseH SYBR Green PCR master mix, 2 μl of primer, 7 μl of PCR-grade water (Takara, Mountain View, Calif., USA) and 1 μl of cDNA was mixed with Power SYBR Green PCR Master Mix (Applied Biosystems) were performed qRT-PCR in a StepOnePlus real-time PCR system. The cycle consisting of 10 minutes at 95 占 폚, 15 seconds at 95 占 폚 and 1 minute at 60 占 폚 was performed 40 times. Fluorescence intensity was measured after each cycle. The Ct (threshold cycle) value of each gene was normalized to the Ct value of β-actin, and the amount of expression of each gene was analyzed by comparing the amount of change in Ct value. The primer sequences of the respective genes used in the experiments are shown in Table 4.

Name of the primer order SEQ ID NO: MMP-1 Forward 5'-ATGCTGAAACCCTGAAGGTG-3 ' SEQ ID NO: 1 Reverse 5'-CTGCTTGACCCTCAGAGACC-3 ' SEQ ID NO: 2 Type I collagen
(Type I collagen) Forward 5'-CTTCCTGCGCCTGATGTCCA-3 ' SEQ ID NO: 3 Reverse 5'-CTCGTGCAGCCATCGACAGT-3 ' SEQ ID NO: 4 β-actin Forward 5'-GGATTCCTATGTGGGCGACGA-3 ' SEQ ID NO: 5 Reverse 5'-CGCTCGGTGAGGATCTTCATG-3 ' SEQ ID NO: 6

Fig. 5 shows the amount of MMP-1 RNA expressed in human skin fibroblasts treated with White mushroom extract 1. As shown in FIG. 5, the expression of MMP-1 was remarkably increased more than 3-fold in the treatment of TNF-α as compared with that of the untreated group, and when treating the mushroom extract 1 (described as AC in FIG. 5) Lt; RTI ID = 0.0 > 50%. ≪ / RTI >

Example 6. Measurement of amount of collagen expression

The relative expression level of type I collagen was measured by the method of Example 5-1 using qRT-PCR. The concentration of TNF-α used and the concentration of the mushroom extract 1 prepared in Example 1-2 were the same as in Example 5-1. The primer sequences used to measure the amount of expression of type I collagen are listed in Table 4 above.

Fig. 6 shows the amount of RNA expression of type I collagen measured in the treatment of white mushroom extract 1 in human skin fibroblasts. As shown in Fig. 6, the expression of type I collagen was reduced by about 25% in the treatment of TNF-α compared to the untreated group, and when treated with white mushroom extract 1 (described as AC in FIG. 6) 1 collagen was increased about 1.5 times or more.

Example 7. Tyrosinase Inhibitory Activity

1.0 ml of a 0.1 M potassium phosphate buffer solution (pH 6.8), 1.0 ml of a 0.3 mg / ml L-tyrosine aqueous solution and 0.1 ml of 1250 unit / ml mushroom tyrosinase (Sigma, USA) were mixed. 100 mu g / ml, 200 mu g / ml, or 400 mu g / ml of the mushroom extract 1 prepared in Example 1-2 was added and reacted at 37 DEG C for 10 minutes. Then, the absorbance of the reaction solution was measured at 480 nm (JASCO V-550, japan), and the tyrosinase inhibitory activity was calculated according to the following formula (3), and it is shown in Table 5 and FIG. As a positive control, kojic acid (koji acid) at a concentration of 50 μg / ml was used.

As shown in Table 5 and FIG. 7, it was found that the mushroom extract of White mushroom inhibited the activity of tyrosinase in a concentration-dependent manner. The addition of 200 ㎍ / ㎖ or 400 ㎍ / ㎖ of white mushroom extract showed higher tyrosinase inhibitory activity than that of positive control kojic acid. The mushroom extract according to one embodiment of the present invention exhibited a tyrosinase inhibitory activity superior to kojic acid when the concentration was increased even though it was not a single purified ingredient.

extract Tyrosinase inhibitory activity (%) Kojic acid (control group) 95.59 + - 0.39 White mushroom extract 1 100 μg / ml 33.58 1.55 Mushroom extract 1 200 μg / ml 47.01 + - 1.97 White mushroom extract 1 400 μg / ml 51.74 + 1.14

Example 8. Preparation of cosmetic composition containing white mushroom extract

Example 8-1. Manufacture of nutrition lotions

3.0 parts by weight of propylene glycol, 0.1 part by weight of carboxy polymer and a trace amount of preservative and remaining amount of purified water were added to 100 parts by weight of the entire composition by heating at 80 to 85 캜 with mixing and stirring, 1.0 part by weight of sorbitan sesquioleate, 10 parts by weight of liquid paraffin, 1.0 part by weight of sorbitan stearate, 0.5 parts by weight of glycerin monostearate as a parent type, 1.5 parts by weight of stearic acid, 0.1 part by weight of glyceryl stearate / PEG- 1.0 part by weight of a catalyst and 0.2 part by weight of triethanolamine were heated to 80 to 85 DEG C and then emulsified. After the emulsification was completed, the mixture was thermally cooled to 50 ° C with stirring using an agitator, and a small amount of a perfume was added thereto. The mixture was cooled to 45 ° C and added with a trace amount of colorant. The white mushroom extract 1 to 5) were added and mixed. The mixture was cooled to 25 占 폚 and stored.

Example 8-2. Manufacture of nutrition cream

0.3 parts by weight of carboxy polymer, 5.0 parts by weight of butylene glycol, 3.0 parts by weight of glycerin and a remaining amount of purified water were added to 100 parts by weight of the entire composition by heating at 80 to 85 캜 with stirring and stirring, , 2.0 parts by weight of cetyl alcohol, 2.0 parts by weight of glyceryl monostearate, 0.5 part by weight of polyoxyethylene sorbitan monostearate, 0.5 parts by weight of sorbitan sesquioleate, 0.5 parts by weight of glyceryl monostearate / glyceryl stearate 1.0 part by weight of polyoxyethylene stearate, 1.0 part by weight of wax, 4.0 parts by weight of liquid paraffin, 4.0 parts by weight of squalane, and 4.0 parts by weight of caprylic / capric triglyceride were heated to 80 to 85 캜 and added with triethanol amine 0.5 And the mixture was emulsified. After completion of the emulsification, 5.0 parts by weight of the white mushroom extract (extracts 1 to 5) prepared in Example 1-2 was added to and mixed with the mixture, and the mixture was cooled to 25 ° C and stored.

Example 9. Evaluation of skin stability

To evaluate the skin stability of the mushroom extract of the present invention, the cream prepared in Example 8-2 was applied to the arm of 49 healthy male and female (24 male, 25 female), and the human skin patch test was carried out Respectively.

Specifically, after the arm of the subject was wiped with 70% ethanol, the cream containing the mushroom extracts 1 to 5 prepared in Example 8-2 was closed for 24 hours by using an adhesive tape in an amount of 20 μl each. After 24 hours, the adhesive tape was removed, and the sample remaining on the skin with a gauze was gently wiped off and the skin condition was observed. After 24 hours and 48 hours, another one was observed.

As a result, skin irritation was not confirmed in the cream containing mushroom extracts 1 to 5, and it was confirmed that the composition according to one embodiment of the present invention was stable irrespective of human skin.

The present invention has been described with reference to the preferred embodiments. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims. Therefore, the disclosed embodiments should be considered in an illustrative rather than a restrictive sense. The scope of the present invention is defined by the appended claims rather than by the foregoing description, and all differences within the scope of equivalents thereof should be construed as being included in the present invention.

<110> UNIJEJU Co., Ltd. <120> Cosmetic Composition comprising Extract of Agaricus blazei <130> PN170370-P1 <150> KR 10-2017-0174568 <151> 2017-12-18 <160> 6 <170> KoPatentin 3.0 <210> 1 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> MMP-1_Forward <400> 1 atgctgaaac cctgaaggtg 20 <210> 2 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> MMP-1_Reverse <400> 2 ctgcttgacc ctcagagacc 20 <210> 3 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Type I collagen_Forward <400> 3 cttcctgcgc ctgatgtcca 20 <210> 4 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Type I collagen_Reverse <400> 4 ctcgtgcagc catcgacagt 20 <210> 5 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> beta-actin_Forward <400> 5 ggattcctat gtgggcgacg a 21 <210> 6 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> beta-actin_Reverse <400> 6 cgctcggtga ggatcttcat g 21

Claims (5)

A cosmetic composition comprising an extract of Agaricus blazei as an active ingredient,
The white mushroom extract,
Washing and drying the white mushroom;
The dried white mushroom was put into an extraction tank, and purified water having a weight of 8 to 10 times the total weight of white mushroom was added, followed by extraction at 110 to 125 ° C for 5 to 9 hours. The first extract was recovered from the extraction tank ;
After addition of purified water having a weight of 8 to 10 times the total weight of the white mushroom remaining in the extraction tank in which the first extract was collected, the extract was extracted at 110 to 125 캜 for 5 to 9 hours, and the second extract ; And
Mixing the recovered first extract and the second extract and filtering them with a filter.
The composition of claim 1, wherein the filter has a pore size of 0.5 to 1.5 탆.
The composition according to claim 1, wherein the cosmetic composition inhibits the activity of at least one enzyme selected from the group consisting of elastase, tyrosinase and collagenase.
The composition according to claim 1, wherein the cosmetic composition has at least one effect selected from the group consisting of wrinkle improvement, skin whitening, skin elasticity enhancement, skin irritation mitigation, and moisturizing effect.
The cosmetic composition according to claim 1, wherein the cosmetic composition is at least one selected from the group consisting of a soft lotion, a gel, a water-soluble liquid, a milk lotion, a cream, an essence, a skin toner, an oil-in-water emulsion, Wherein the composition is a formulation selected from the group consisting of a cleansing foam, a cleansing water, a pack, a body oil, a body lotion, an underwater type makeup base, a water-in-oil type makeup base and a foundation.

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