FR2566911A1 - Sonde et procede pour le diagnostic du sexe d'un embryon humain - Google Patents
Sonde et procede pour le diagnostic du sexe d'un embryon humain Download PDFInfo
- Publication number
- FR2566911A1 FR2566911A1 FR8410408A FR8410408A FR2566911A1 FR 2566911 A1 FR2566911 A1 FR 2566911A1 FR 8410408 A FR8410408 A FR 8410408A FR 8410408 A FR8410408 A FR 8410408A FR 2566911 A1 FR2566911 A1 FR 2566911A1
- Authority
- FR
- France
- Prior art keywords
- probe
- dna
- cells
- fragment
- hybridization
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 238000000034 method Methods 0.000 title claims abstract description 28
- 238000003745 diagnosis Methods 0.000 title claims description 7
- 210000001161 mammalian embryo Anatomy 0.000 title abstract description 11
- 239000012634 fragment Substances 0.000 claims abstract description 24
- 238000009396 hybridization Methods 0.000 claims abstract description 20
- 210000004027 cell Anatomy 0.000 claims abstract description 19
- 150000007523 nucleic acids Chemical class 0.000 claims abstract description 12
- 210000000349 chromosome Anatomy 0.000 claims abstract description 11
- 108020004707 nucleic acids Proteins 0.000 claims abstract description 11
- 102000039446 nucleic acids Human genes 0.000 claims abstract description 11
- 238000000338 in vitro Methods 0.000 claims abstract description 10
- 238000001514 detection method Methods 0.000 claims abstract description 5
- 244000005700 microbiome Species 0.000 claims abstract description 3
- 108020004414 DNA Proteins 0.000 claims description 33
- 239000000523 sample Substances 0.000 claims description 32
- 210000002593 Y chromosome Anatomy 0.000 claims description 10
- 210000002257 embryonic structure Anatomy 0.000 claims description 8
- 238000002405 diagnostic procedure Methods 0.000 claims description 7
- 238000002360 preparation method Methods 0.000 claims description 7
- 210000003754 fetus Anatomy 0.000 claims description 5
- 238000005406 washing Methods 0.000 claims description 5
- 108010077544 Chromatin Proteins 0.000 claims description 4
- 210000003483 chromatin Anatomy 0.000 claims description 4
- 239000003298 DNA probe Substances 0.000 claims description 3
- 108020004635 Complementary DNA Proteins 0.000 claims description 2
- 238000010804 cDNA synthesis Methods 0.000 claims description 2
- 230000002759 chromosomal effect Effects 0.000 claims description 2
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- 239000000243 solution Substances 0.000 description 22
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 10
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- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 9
- 238000012360 testing method Methods 0.000 description 8
- 239000000020 Nitrocellulose Substances 0.000 description 7
- FJWGYAHXMCUOOM-QHOUIDNNSA-N [(2s,3r,4s,5r,6r)-2-[(2r,3r,4s,5r,6s)-4,5-dinitrooxy-2-(nitrooxymethyl)-6-[(2r,3r,4s,5r,6s)-4,5,6-trinitrooxy-2-(nitrooxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-3,5-dinitrooxy-6-(nitrooxymethyl)oxan-4-yl] nitrate Chemical compound O([C@@H]1O[C@@H]([C@H]([C@H](O[N+]([O-])=O)[C@H]1O[N+]([O-])=O)O[C@H]1[C@@H]([C@@H](O[N+]([O-])=O)[C@H](O[N+]([O-])=O)[C@@H](CO[N+]([O-])=O)O1)O[N+]([O-])=O)CO[N+](=O)[O-])[C@@H]1[C@@H](CO[N+]([O-])=O)O[C@@H](O[N+]([O-])=O)[C@H](O[N+]([O-])=O)[C@H]1O[N+]([O-])=O FJWGYAHXMCUOOM-QHOUIDNNSA-N 0.000 description 7
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- 210000002993 trophoblast Anatomy 0.000 description 7
- 108010034791 Heterochromatin Proteins 0.000 description 6
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- 238000001574 biopsy Methods 0.000 description 5
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- 238000004458 analytical method Methods 0.000 description 4
- 238000001914 filtration Methods 0.000 description 4
- 108091008146 restriction endonucleases Proteins 0.000 description 4
- 239000011780 sodium chloride Substances 0.000 description 4
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- 210000001766 X chromosome Anatomy 0.000 description 3
- 210000004369 blood Anatomy 0.000 description 3
- 239000008280 blood Substances 0.000 description 3
- 210000003917 human chromosome Anatomy 0.000 description 3
- 230000035935 pregnancy Effects 0.000 description 3
- 108010067770 Endopeptidase K Proteins 0.000 description 2
- 108091028043 Nucleic acid sequence Proteins 0.000 description 2
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
- 238000006935 Simonis synthesis reaction Methods 0.000 description 2
- 210000000601 blood cell Anatomy 0.000 description 2
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- 230000001605 fetal effect Effects 0.000 description 2
- 210000002950 fibroblast Anatomy 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- LWGJTAZLEJHCPA-UHFFFAOYSA-N n-(2-chloroethyl)-n-nitrosomorpholine-4-carboxamide Chemical compound ClCCN(N=O)C(=O)N1CCOCC1 LWGJTAZLEJHCPA-UHFFFAOYSA-N 0.000 description 2
- 230000003169 placental effect Effects 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
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- 238000002604 ultrasonography Methods 0.000 description 2
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 108010022894 Euchromatin Proteins 0.000 description 1
- 208000034454 F12-related hereditary angioedema with normal C1Inh Diseases 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- 101500006448 Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) Endonuclease PI-MboI Proteins 0.000 description 1
- 108020002230 Pancreatic Ribonuclease Proteins 0.000 description 1
- 102000005891 Pancreatic ribonuclease Human genes 0.000 description 1
- 239000012980 RPMI-1640 medium Substances 0.000 description 1
- 244000171726 Scotch broom Species 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- 230000005856 abnormality Effects 0.000 description 1
- 206010000210 abortion Diseases 0.000 description 1
- 231100000176 abortion Toxicity 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 235000019270 ammonium chloride Nutrition 0.000 description 1
- 238000002669 amniocentesis Methods 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 210000002230 centromere Anatomy 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 210000001136 chorion Anatomy 0.000 description 1
- 239000003593 chromogenic compound Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000002224 dissection Methods 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 210000000632 euchromatin Anatomy 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000012894 fetal calf serum Substances 0.000 description 1
- 238000013467 fragmentation Methods 0.000 description 1
- 238000006062 fragmentation reaction Methods 0.000 description 1
- 208000016861 hereditary angioedema type 3 Diseases 0.000 description 1
- 210000004090 human X chromosome Anatomy 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 230000016507 interphase Effects 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 210000000265 leukocyte Anatomy 0.000 description 1
- 230000008774 maternal effect Effects 0.000 description 1
- 235000019799 monosodium phosphate Nutrition 0.000 description 1
- 210000004940 nucleus Anatomy 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 210000002826 placenta Anatomy 0.000 description 1
- 239000013612 plasmid Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000002285 radioactive effect Effects 0.000 description 1
- 108091035233 repetitive DNA sequence Proteins 0.000 description 1
- 102000053632 repetitive DNA sequence Human genes 0.000 description 1
- 230000003252 repetitive effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 230000020509 sex determination Effects 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 description 1
- 229910000162 sodium phosphate Inorganic materials 0.000 description 1
- ILXAOQAXSHVHTM-UHFFFAOYSA-M sodium;2-amino-2-(hydroxymethyl)propane-1,3-diol;chloride Chemical compound [Na+].[Cl-].OCC(N)(CO)CO ILXAOQAXSHVHTM-UHFFFAOYSA-M 0.000 description 1
- 239000008279 sol Substances 0.000 description 1
- 210000001082 somatic cell Anatomy 0.000 description 1
- 238000000527 sonication Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 238000009210 therapy by ultrasound Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 229950003937 tolonium Drugs 0.000 description 1
- HNONEKILPDHFOL-UHFFFAOYSA-M tolonium chloride Chemical compound [Cl-].C1=C(C)C(N)=CC2=[S+]C3=CC(N(C)C)=CC=C3N=C21 HNONEKILPDHFOL-UHFFFAOYSA-M 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 230000036266 weeks of gestation Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6879—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for sex determination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Priority Applications (6)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR8410408A FR2566911A1 (fr) | 1984-06-29 | 1984-06-29 | Sonde et procede pour le diagnostic du sexe d'un embryon humain |
| GR851598A GR851598B (enrdf_load_stackoverflow) | 1984-06-29 | 1985-06-28 | |
| ES544664A ES8608683A1 (es) | 1984-06-29 | 1985-06-28 | Procedimiento para el diagnostico in vitro del sexo |
| EP85903297A EP0188485A1 (fr) | 1984-06-29 | 1985-06-28 | Sonde et procede pour le diagnostic du sexe d'un embryon humain |
| PT80743A PT80743B (fr) | 1984-06-29 | 1985-06-28 | Sonde et procede pour le diagnostic du sexe d'un embryon humain |
| PCT/FR1985/000178 WO1986000342A1 (fr) | 1984-06-29 | 1985-06-28 | Sonde et procede pour le diagnostic du sexe d'un embryon humain |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR8410408A FR2566911A1 (fr) | 1984-06-29 | 1984-06-29 | Sonde et procede pour le diagnostic du sexe d'un embryon humain |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| FR2566911A1 true FR2566911A1 (fr) | 1986-01-03 |
Family
ID=9305658
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| FR8410408A Withdrawn FR2566911A1 (fr) | 1984-06-29 | 1984-06-29 | Sonde et procede pour le diagnostic du sexe d'un embryon humain |
Country Status (6)
| Country | Link |
|---|---|
| EP (1) | EP0188485A1 (enrdf_load_stackoverflow) |
| ES (1) | ES8608683A1 (enrdf_load_stackoverflow) |
| FR (1) | FR2566911A1 (enrdf_load_stackoverflow) |
| GR (1) | GR851598B (enrdf_load_stackoverflow) |
| PT (1) | PT80743B (enrdf_load_stackoverflow) |
| WO (1) | WO1986000342A1 (enrdf_load_stackoverflow) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4769319A (en) * | 1985-05-31 | 1988-09-06 | Salk Institute Biotechnology Industrial Associates, Inc. | Nucleic acid probes for prenatal sexing |
| EP0261172A1 (en) * | 1986-02-21 | 1988-03-30 | Whitehead Institute For Biomedical Research | Y-specific dna hybridization probes and uses therefor |
| FR2595100B1 (fr) * | 1986-02-28 | 1990-02-09 | Agronomique Inst Nat Rech | Sondes moleculaires d'adn specifique du genome male de mammiferes, notamment du genre bos, presentant des sequences d'adn specifique du sexe male, procede de preparation de telles sondes et procede de determination du sexe d'embryons et de foetus des mammiferes susdits, a l'aide de ces sondes |
| FR2603701B2 (fr) * | 1986-02-28 | 1990-09-21 | Agronomique Inst Nat Rech | Applications des sondes moleculaires d'adn specifique du genome male de mammiferes, notamment du genre bos, au controle de la presence du chromosome y dans une population de spermatozoides et a la separation des spermatozoides en deux populations de spermatozoides porteurs respectivement du chromosome y et du chromosome x pour leur utilisation pour l'insemination artificielle |
| ATE87979T1 (de) * | 1986-08-12 | 1993-04-15 | Univ Australian | Geschlechtsbestimmung bei wiederkaeuern unter verwendung y-chromosomenspezifischer polynukleotide. |
| US5328827A (en) * | 1987-08-27 | 1994-07-12 | Institut National De La Recherche Agronomique - Inra | Specific DNA molecular probes for Bos-type male genome |
| FR2619819B1 (fr) * | 1987-08-27 | 1990-08-10 | Agronomique Inst Nat Rech | Sondes moleculaires d'adn specifique du genome male de ruminants, notamment de la sous-famille des bovines et en particulier du genre bos, sequences flanquantes pour l'amplification de ces sondes et applications desdites sondes |
| WO1989002440A2 (en) * | 1987-09-21 | 1989-03-23 | Whitehead Institute For Biomedical Research | Y-specific dna hybridization probes and uses therefor |
| DE68926668T2 (de) * | 1988-01-29 | 1997-02-13 | Advanced Riverina Holdings | Bestimmung des geschlechts bei wiederkäuern unter verwendung von gamma-chromosom-spezifischen polynukleotiden |
| JP2693776B2 (ja) * | 1988-02-24 | 1997-12-24 | 科学技術振興事業団 | Ｄｎａプローブ及びそれを用いたヒト胎児の性別判定法 |
| FR2635116B1 (fr) * | 1988-08-08 | 1990-11-02 | Georges Michel | Procede pour la determination du sexe d'embryons de ruminants, kit ou necessaire pour la mise en oeuvre du procede |
| US5840482A (en) * | 1990-10-10 | 1998-11-24 | The Regents Of The University Of California | Y chromosome specific nucleic acid probe and method for determining the Y chromosome in situ |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE735915C (de) * | 1939-07-27 | 1943-06-01 | Waffen Und Munitionsfabriken A | Vorrichtung zum gleichzeitigen Messen der Wandstaerke und deren Verlauf laengs des Umfanges von zylindrischen Hohlkoerpern an mindestens drei verschiedenen Stellen |
| DE2909836A1 (de) * | 1978-03-17 | 1979-09-27 | Johde Fa | Praezisionsmessvorrichtung fuer zylindrische objekte |
| DD154846A1 (de) * | 1980-11-27 | 1982-04-21 | Ursula Barth | Verfahren und auswerteeinrichtung zur angenaeherten paarungsdurchmesserbestimmung rotationssymmetrischer teile |
-
1984
- 1984-06-29 FR FR8410408A patent/FR2566911A1/fr not_active Withdrawn
-
1985
- 1985-06-28 ES ES544664A patent/ES8608683A1/es not_active Expired
- 1985-06-28 GR GR851598A patent/GR851598B/el unknown
- 1985-06-28 WO PCT/FR1985/000178 patent/WO1986000342A1/fr not_active Application Discontinuation
- 1985-06-28 EP EP85903297A patent/EP0188485A1/fr not_active Withdrawn
- 1985-06-28 PT PT80743A patent/PT80743B/pt unknown
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE735915C (de) * | 1939-07-27 | 1943-06-01 | Waffen Und Munitionsfabriken A | Vorrichtung zum gleichzeitigen Messen der Wandstaerke und deren Verlauf laengs des Umfanges von zylindrischen Hohlkoerpern an mindestens drei verschiedenen Stellen |
| DE2909836A1 (de) * | 1978-03-17 | 1979-09-27 | Johde Fa | Praezisionsmessvorrichtung fuer zylindrische objekte |
| DD154846A1 (de) * | 1980-11-27 | 1982-04-21 | Ursula Barth | Verfahren und auswerteeinrichtung zur angenaeherten paarungsdurchmesserbestimmung rotationssymmetrischer teile |
Non-Patent Citations (4)
| Title |
|---|
| CHEMICAL ABSTRACTS, vol. 100, no. 11, 12 mars 1984, page 255, réf. no. 82189p, Columbus, Ohio, US; Y.F. LAU et al.: "A rapid screening test for antenatal sex determination" & LANCET 1984, 1(8367), 14-16 * |
| CHEMICAL ABSTRACTS, vol. 101, no. 1, 2 juillet 1984, page 296, réf. no. 3425g, Columbus, Ohio, US; J.R. GOSDEN et a.: "Rapid fetal sex determination in first trimester prenatal diagnosis by dot hybridization of DNA probes" & LANCET 1984, 1(8376), 540-1 * |
| NATURE, vol. 307, no. 5947, 12 janvier 1984, pages 172-173, MacMillan Journals Ltd., Londres, GB; G. GUELLAEN et al.: "Human XX males with Y single-copy DNA fragments" * |
| PROC. NATL. ACAD. SCI. USA, vol. 80, décembre 1983, pages 7571-7575; A. BERNHEIM et al.: "Direct hybridization of sorted human chromosomes: Localization of the Y chromosome on the flow karyotype" * |
Also Published As
| Publication number | Publication date |
|---|---|
| ES8608683A1 (es) | 1986-07-16 |
| ES544664A0 (es) | 1986-07-16 |
| EP0188485A1 (fr) | 1986-07-30 |
| PT80743B (fr) | 1986-12-11 |
| GR851598B (enrdf_load_stackoverflow) | 1985-11-25 |
| PT80743A (fr) | 1985-07-01 |
| WO1986000342A1 (fr) | 1986-01-16 |
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