PT80743B - Sonde et procede pour le diagnostic du sexe d'un embryon humain - Google Patents
Sonde et procede pour le diagnostic du sexe d'un embryon humain Download PDFInfo
- Publication number
- PT80743B PT80743B PT80743A PT8074385A PT80743B PT 80743 B PT80743 B PT 80743B PT 80743 A PT80743 A PT 80743A PT 8074385 A PT8074385 A PT 8074385A PT 80743 B PT80743 B PT 80743B
- Authority
- PT
- Portugal
- Prior art keywords
- aon
- sex
- probe
- fragment
- cells
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims abstract description 26
- 238000003745 diagnosis Methods 0.000 title claims description 9
- 210000001161 mammalian embryo Anatomy 0.000 title abstract description 10
- 239000000523 sample Substances 0.000 claims abstract description 32
- 239000012634 fragment Substances 0.000 claims abstract description 23
- 210000004027 cell Anatomy 0.000 claims abstract description 19
- 210000000349 chromosome Anatomy 0.000 claims abstract description 14
- 238000000338 in vitro Methods 0.000 claims abstract description 7
- 244000005700 microbiome Species 0.000 claims abstract description 3
- 238000009396 hybridization Methods 0.000 claims description 19
- 210000003754 fetus Anatomy 0.000 claims description 5
- 210000003917 human chromosome Anatomy 0.000 claims description 4
- 238000005406 washing Methods 0.000 claims description 4
- 230000000295 complement effect Effects 0.000 claims 1
- 230000009089 cytolysis Effects 0.000 claims 1
- 150000007523 nucleic acids Chemical class 0.000 abstract description 8
- 108020004707 nucleic acids Proteins 0.000 abstract description 7
- 102000039446 nucleic acids Human genes 0.000 abstract description 7
- 238000001514 detection method Methods 0.000 abstract description 2
- 239000000243 solution Substances 0.000 description 23
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- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 8
- 238000002474 experimental method Methods 0.000 description 7
- 108020004414 DNA Proteins 0.000 description 6
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- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 6
- 239000007983 Tris buffer Substances 0.000 description 6
- 235000019333 sodium laurylsulphate Nutrition 0.000 description 6
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 6
- 108010034791 Heterochromatin Proteins 0.000 description 5
- 210000002593 Y chromosome Anatomy 0.000 description 5
- 210000004458 heterochromatin Anatomy 0.000 description 5
- 210000001182 human Y chromosome Anatomy 0.000 description 5
- 238000002360 preparation method Methods 0.000 description 5
- 238000001574 biopsy Methods 0.000 description 4
- 238000002405 diagnostic procedure Methods 0.000 description 4
- 210000002257 embryonic structure Anatomy 0.000 description 4
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- 239000011780 sodium chloride Substances 0.000 description 4
- 229940083575 sodium dodecyl sulfate Drugs 0.000 description 4
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- 238000003556 assay Methods 0.000 description 3
- 238000001914 filtration Methods 0.000 description 3
- 108091008146 restriction endonucleases Proteins 0.000 description 3
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- 241000212384 Bifora Species 0.000 description 2
- 108010077544 Chromatin Proteins 0.000 description 2
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 2
- 239000000020 Nitrocellulose Substances 0.000 description 2
- 210000001766 X chromosome Anatomy 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 230000001013 cariogenic effect Effects 0.000 description 2
- 210000004252 chorionic villi Anatomy 0.000 description 2
- 210000003483 chromatin Anatomy 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- 244000078703 ectoparasite Species 0.000 description 2
- 239000000706 filtrate Substances 0.000 description 2
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- 229920001220 nitrocellulos Polymers 0.000 description 2
- 230000003169 placental effect Effects 0.000 description 2
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- 230000005945 translocation Effects 0.000 description 2
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- 238000009631 Broth culture Methods 0.000 description 1
- VYZAMTAEIAYCRO-UHFFFAOYSA-N Chromium Chemical compound [Cr] VYZAMTAEIAYCRO-UHFFFAOYSA-N 0.000 description 1
- 108020004635 Complementary DNA Proteins 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- 108020002230 Pancreatic Ribonuclease Proteins 0.000 description 1
- 102000005891 Pancreatic ribonuclease Human genes 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- 208000024967 X-linked recessive disease Diseases 0.000 description 1
- 206010000210 abortion Diseases 0.000 description 1
- 231100000176 abortion Toxicity 0.000 description 1
- 238000002669 amniocentesis Methods 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 210000000601 blood cell Anatomy 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 238000010804 cDNA synthesis Methods 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 210000002230 centromere Anatomy 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 229910052804 chromium Inorganic materials 0.000 description 1
- 239000011651 chromium Substances 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 239000002299 complementary DNA Substances 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 230000008021 deposition Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000002224 dissection Methods 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 210000001671 embryonic stem cell Anatomy 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 235000019439 ethyl acetate Nutrition 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000012894 fetal calf serum Substances 0.000 description 1
- 230000001605 fetal effect Effects 0.000 description 1
- 210000002950 fibroblast Anatomy 0.000 description 1
- 238000003818 flash chromatography Methods 0.000 description 1
- 238000013467 fragmentation Methods 0.000 description 1
- 238000006062 fragmentation reaction Methods 0.000 description 1
- 238000003304 gavage Methods 0.000 description 1
- 210000004090 human X chromosome Anatomy 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 230000008774 maternal effect Effects 0.000 description 1
- GQPLMRYTRLFLPF-UHFFFAOYSA-N nitrous oxide Inorganic materials [O-][N+]#N GQPLMRYTRLFLPF-UHFFFAOYSA-N 0.000 description 1
- 210000004940 nucleus Anatomy 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000003793 prenatal diagnosis Methods 0.000 description 1
- 229940002612 prodrug Drugs 0.000 description 1
- 239000000651 prodrug Substances 0.000 description 1
- 235000019833 protease Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000002285 radioactive effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 230000020509 sex determination Effects 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- 210000001082 somatic cell Anatomy 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 229950003937 tolonium Drugs 0.000 description 1
- HNONEKILPDHFOL-UHFFFAOYSA-M tolonium chloride Chemical compound [Cl-].C1=C(C)C(N)=CC2=[S+]C3=CC(N(C)C)=CC=C3N=C21 HNONEKILPDHFOL-UHFFFAOYSA-M 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 238000002604 ultrasonography Methods 0.000 description 1
- 239000013598 vector Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6879—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for sex determination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR8410408A FR2566911A1 (fr) | 1984-06-29 | 1984-06-29 | Sonde et procede pour le diagnostic du sexe d'un embryon humain |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| PT80743A PT80743A (fr) | 1985-07-01 |
| PT80743B true PT80743B (fr) | 1986-12-11 |
Family
ID=9305658
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PT80743A PT80743B (fr) | 1984-06-29 | 1985-06-28 | Sonde et procede pour le diagnostic du sexe d'un embryon humain |
Country Status (6)
| Country | Link |
|---|---|
| EP (1) | EP0188485A1 (enrdf_load_stackoverflow) |
| ES (1) | ES8608683A1 (enrdf_load_stackoverflow) |
| FR (1) | FR2566911A1 (enrdf_load_stackoverflow) |
| GR (1) | GR851598B (enrdf_load_stackoverflow) |
| PT (1) | PT80743B (enrdf_load_stackoverflow) |
| WO (1) | WO1986000342A1 (enrdf_load_stackoverflow) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4769319A (en) * | 1985-05-31 | 1988-09-06 | Salk Institute Biotechnology Industrial Associates, Inc. | Nucleic acid probes for prenatal sexing |
| EP0261172A1 (en) * | 1986-02-21 | 1988-03-30 | Whitehead Institute For Biomedical Research | Y-specific dna hybridization probes and uses therefor |
| FR2595100B1 (fr) * | 1986-02-28 | 1990-02-09 | Agronomique Inst Nat Rech | Sondes moleculaires d'adn specifique du genome male de mammiferes, notamment du genre bos, presentant des sequences d'adn specifique du sexe male, procede de preparation de telles sondes et procede de determination du sexe d'embryons et de foetus des mammiferes susdits, a l'aide de ces sondes |
| FR2603701B2 (fr) * | 1986-02-28 | 1990-09-21 | Agronomique Inst Nat Rech | Applications des sondes moleculaires d'adn specifique du genome male de mammiferes, notamment du genre bos, au controle de la presence du chromosome y dans une population de spermatozoides et a la separation des spermatozoides en deux populations de spermatozoides porteurs respectivement du chromosome y et du chromosome x pour leur utilisation pour l'insemination artificielle |
| ATE87979T1 (de) * | 1986-08-12 | 1993-04-15 | Univ Australian | Geschlechtsbestimmung bei wiederkaeuern unter verwendung y-chromosomenspezifischer polynukleotide. |
| US5328827A (en) * | 1987-08-27 | 1994-07-12 | Institut National De La Recherche Agronomique - Inra | Specific DNA molecular probes for Bos-type male genome |
| FR2619819B1 (fr) * | 1987-08-27 | 1990-08-10 | Agronomique Inst Nat Rech | Sondes moleculaires d'adn specifique du genome male de ruminants, notamment de la sous-famille des bovines et en particulier du genre bos, sequences flanquantes pour l'amplification de ces sondes et applications desdites sondes |
| WO1989002440A2 (en) * | 1987-09-21 | 1989-03-23 | Whitehead Institute For Biomedical Research | Y-specific dna hybridization probes and uses therefor |
| DE68926668T2 (de) * | 1988-01-29 | 1997-02-13 | Advanced Riverina Holdings | Bestimmung des geschlechts bei wiederkäuern unter verwendung von gamma-chromosom-spezifischen polynukleotiden |
| JP2693776B2 (ja) * | 1988-02-24 | 1997-12-24 | 科学技術振興事業団 | Ｄｎａプローブ及びそれを用いたヒト胎児の性別判定法 |
| FR2635116B1 (fr) * | 1988-08-08 | 1990-11-02 | Georges Michel | Procede pour la determination du sexe d'embryons de ruminants, kit ou necessaire pour la mise en oeuvre du procede |
| US5840482A (en) * | 1990-10-10 | 1998-11-24 | The Regents Of The University Of California | Y chromosome specific nucleic acid probe and method for determining the Y chromosome in situ |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE735915C (de) * | 1939-07-27 | 1943-06-01 | Waffen Und Munitionsfabriken A | Vorrichtung zum gleichzeitigen Messen der Wandstaerke und deren Verlauf laengs des Umfanges von zylindrischen Hohlkoerpern an mindestens drei verschiedenen Stellen |
| NO149296C (no) * | 1978-03-17 | 1984-03-21 | Johde Fa | Maaleapparat for sylinderformete gjenstander |
| DD154846B1 (de) * | 1980-11-27 | 1987-03-11 | Ursula Barth | Verfahren und auswerteeinrichtung zur angenaeherten paarungsdurchmesserbestimmung rotationssymmetrischer teile |
-
1984
- 1984-06-29 FR FR8410408A patent/FR2566911A1/fr not_active Withdrawn
-
1985
- 1985-06-28 ES ES544664A patent/ES8608683A1/es not_active Expired
- 1985-06-28 GR GR851598A patent/GR851598B/el unknown
- 1985-06-28 WO PCT/FR1985/000178 patent/WO1986000342A1/fr not_active Application Discontinuation
- 1985-06-28 EP EP85903297A patent/EP0188485A1/fr not_active Withdrawn
- 1985-06-28 PT PT80743A patent/PT80743B/pt unknown
Also Published As
| Publication number | Publication date |
|---|---|
| ES8608683A1 (es) | 1986-07-16 |
| ES544664A0 (es) | 1986-07-16 |
| EP0188485A1 (fr) | 1986-07-30 |
| GR851598B (enrdf_load_stackoverflow) | 1985-11-25 |
| PT80743A (fr) | 1985-07-01 |
| WO1986000342A1 (fr) | 1986-01-16 |
| FR2566911A1 (fr) | 1986-01-03 |
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