FR2565485A1 - STABLE TOOTHPASTE CONTAINING DEXTRANASE - Google Patents

Abstract

STABLE COMPOSITION FOR ORAL USE CONTAINING THE ENZYME DEXTRANASE, AN ANIONIC SURFACTANT SYSTEM, PREFERABLY CONTAINING AN AMPHOTERIC OR NON-IONIC SURFACTANT AND A POLYCATION STABILIZER SELECTED AMONG PROTEINS, POLYPEPTIDES AND POLYINS IN THE PRESENCE OF THE ANIONIC SURFACTANT WITHOUT DAMAGING ITS FOAMING AND CLEANING PROPERTIES.

Description

The present invention relates to new com

  positions for oral use comprising enzymes of the type

  dextranase, which are enzymatically stabilized

  that, physical and cosmetic in the presence of an anionic surfactant by addition of a polycationic stabilizer

  chosen from proteins, polypeptides and poly-

  amines, which prevent inactivation of dextranase and promote the maintenance of enzyme activity over an extended period. The presence of the amphoteric surfactant

  helps to ensure the stability of the composition.

  The prior art is replete with information regarding the efficacy of dextranase against dental plaque for dispersing and eliminating it, as shown in an article by Bowen in the British Dental Journal, vol. 24, N 8, 347-349 (April 16, 1968); an article by Fitzgerald et al. in JADA, vol. 76,

  301-304 (February 1968); and an article by Duany et al.

  in the Journal of Preventive dentistry, vol. 2, N 2, 23-27 (March-April 1975). Dextranase-type enzymes also decrease the formation of dental caries and periodontal disease when applied topically. These enzymes degrade or break down the dextrans synthesized in the plate from sucrose by Strep. mutans. Dextrans serve as glue for

cohesion of the plate.

  Therefore, dextranase has been incorporated into conventional oral hygiene products such as

  toothpaste, rinses and chewing-

  gum containing cleaning and foaming surfactants

  health, as shown in Japanese patent publication N 56834/1973 and containing an anionic surfactant such as

  The British patent N 1 319 423 shows this.

  Active agents, in particular anionic surfactants, tend to deactivate enzymes such as dextranase, which causes a rapid drop in enzyme activity in the absence of stabilization. It is therefore difficult to make a stable and foaming toothpaste containing

  dextranase. Therefore, a certain name has been incorporated-

  number of stabilizers in toothpaste compositions containing

  dextranase. For example, US Patent 3,991,177 teaches the use of manganese ions and ions

  calcium to stabilize dextranases in dentifri-

  these in the presence of an anionic surfactant such as N-

  sodium lauroylsarcosinate. US Pat. No. 3,981,989 describes the use of gelatin or peptone as stabilizing agents for dextranases in the presence of sodium lauryl sulfate. US Patent No. 4,140,758 teaches the use of a metal ion selected from manganese, calcium, magnesium and mixtures thereof as a stabilizer activating dextranase. Japanese Patent No. 013318 to Lion, dated February 6, 1980, uses eugenol and 1-menthol as a stabilizer for dextranase in the presence of arionic surfactants. Japanese Patent No. 010350 to Lion Corp., dated January 27, 1981, uses a mixture of dextranase and omega-amino acids in compositions

  for oral use to prevent the formation of bac-

  terian. French patent application N 82/05799 describes a composition for oral use comprising a mixture of dextranase and alpha-1,3-glucanase. The patent application

  C-B N 2 061 727A uses aluminas and aluminas

  dratées as abrasive, to stabilize the dextranase in

the toothpaste composition.

  US Patent No. 3,562,385 describes compositions for dental antiplaque and anti-scaling containing a mixture of a compound with bisguanido group, dextranase and sodium hexametaphosphate. US Patent No. 3,622,661 describes preparations for oral use containing dextranase and a specific binder carragheen or gum adra-

  glove to prevent demixing in the long run. Compounds

  anti-plaque and / or anti-tartar oral use

  dextranase are also described in US Pat. Nos. 3,630,924; 3,686,393; and 3,751,561. Oral compositions containing dextranase associated with

  other enzymes are described in US Patent No. 4,335,101.

  Dextranase was treated by molecular modification using a phosphoprotein excipient and a reagent as

  ethyl chloroformate to ensure longer

  activity in the oral cavity, as defined

  written in US Patent No. 4,138,476.

  Cosmetically stable toothpaste

  metabolic and enzymatic, containing a neutral protease of B. subtilis, stabilized by a partially protein

  hydrolyzed are described in US Patent No. 4,058,596.

  The addition of a group IIA metal ion to the combi-

  The addition of neutral protease and hydrolyzed protein provides additional stability as described in the

U.S. Patent No. 4,058,595.

  Although the problem of stabilizing dextranase in the presence of anionic surfactants is well known in the prior art, and has been resolved by the addition of various stabilizing agents as well as

  was mentioned above, no description found

  the use of a polycationic stabilizer chosen from proteins, polypeptides and polyamines, which

  more specifically includes a hydrolyzed protein which

  tarnished (Crotein Q), polylysine, polyarginine,

  protamine sulfate, a polyacryloxyalkylammo- salt

  nium, a salt of polyvinylpyridinium, polyoxyethylene

  (dimethylamino) dichloroethane (Busan-77) and poly N- (2-

hydroxypropyl) methacrylamide.

  It was unexpectedly discovered that the addi-

  tion of a polycationic stabilizer, chosen from proteins, peptides and polyamines, to a toothpaste containing an enzyme of the dextranase type and one or more anionic surfactants, preferably associated with

  amphoteric or non-ionic surfactants, provides better

  their stability on storage of the enzyme as gelatin and the other enzyme stabilizers of the prior art,

  without harming the foaming power or the detergency of the

anionic surfactant.

  Therefore, a main object of the present invention is to provide a stable toothpaste containing an anionic surfactant, a dextranase enzyme and

a polycationic stabilizer.

  Another object of the present invention is to provide

  a toothpaste containing foaming dextranase and

  stable in the presence of anionic and non-ionic surfactants

  ques. Yet another object of the present invention is

  to provide a toothpaste containing soft dextranase

  healthy and stable in the presence of anionic surfactants and

amphoteric.

  Another object of the present invention is to provide

  a toothpaste containing dextranase, stable from

  physical and cosmetic point of view, and in which the ac-

  The activity of the enzyme is retained during aging.

  Another great object of the present invention is to provide a stable toothpaste containing dextranase

  without harming the foaming properties of the tensio system

anionic active.

  Other objects, advantages and new features of the invention will be explained in part in the

  description below and in part will become apparent to

  those skilled in the art considering what may or may be

  learned by practicing the invention. goals and before-

  ges of the invention can be achieved and achieved by the means and combinations particularly emphasized in

the claims appended.

  To achieve the above goals and others

  aims and according to the present invention as it is

  read and described in general, the stable toothpaste

  of the present invention includes a tensio-ac system

  anionic tif, a dextranase and a polycatio stabilizer

  selected from proteins, polypeptides and

  polyamines, in an excipient for dental use. The sta-

  typical bilisants can be chosen from a derivative

  of quaternary ammonium from a hydrolyzed protein, the poly-

  lysine, polyarginine, protamine sulfate, a polyacryloxyalkylammonium salt, a polyvinylpyridinium salt

  polyoxyethylene (dimethylamino) dichloro-

  ethane and poly (N-2-hydroxypropyl) methacrylamide.

  More specifically, the present invention

  identifies a stable composition for oral use which can be in the form of a powder, paste, cream, liquid or chewing gum, comprising an anionic surfactant system, a dextranase in quantity that can

  provide 1,000 to 55,000 units per gram of enzyme activity

  initial tick, and a polycationic stabilizer in a weight ratio of 1/1 to 1/2 of the anionic surfactant to the stabilizer. Dextranase enzymes are produced from a variety of sources, all of which are useful in the present invention. Dextranase enzymes are commonly produced by culturing Penicillium funiculosum or other fungal sources in a medium containing dextran. Dextran is usually a product of

  commercial quality obtained from Leuconostoc mesente-

  rioides. This commercial grade of dextran contains

  about 95% of alpha-l, 6-glucoside links and about 5% of alpha-l, 3-glucoside links. The body of Penicillium produces dextranase which in particular hydrolyzes the

1.6 links.

  Dextranase can also be prepared by

  methods which are described in the prior art.

  These include the process described by Bowen, "British Dental Journal", vol. 124, N 8, dated April 16, 1968, 343-349. Another method is described in US Patent No. 2,742,399 to Tsuchiya et al. (see also Tsuchiya and

  coll., "Journal of Bacteriology", vol. 64, page 513).

  In Bowen's process, dextran can be prepared from non-cariogenic streptococcal strains

  such as ATCC 10558, 903-1600, IIA2 + 3, or Leuconostoc mesen-

  terioides and purified by the method described by Wood et al., "Archives of Oral Biology", vol. 11, 1066, pages

  1039 and following, except that L. mesente-

rioides is grown at 25 C.

  Dextranase can be prepared for the inoculation of Penicillium funiculosum in vials containing 250 ml of medium containing 0.5% yeast extract and 1% dextran. The flasks are incubated at 30 ° C in a shaking incubator for 4 days. Then the culture is centrifuged at 3000 g for

  minutes and filtered on Whatman 42 filter paper.

  Then dialyze in a 16mm "Visking" tube system with deionized water and concentrate

  times by dialysis with polyethylene glycol (molar-

  20,000). The dextranase produced by this process has a

  molecular weight of about 200,000 to 275,000. If we de-

  sire, we can still purify dextranase by fraction-

ammonium sulfate.

  Other processes for preparing dextra-

  nase include the one described in the US patent

No. 2,742,399 to Tsuchiya et al.

  Dextranases of bacterial origin can also be used in the compositions of the present invention. Dextranase of bacterial origin can be prepared by the general method by which enzymes are produced from bacteria. However, the source

  preferred dextranase for the purposes of this invention

  tion is a mutant of Bacillus coagulans, NRRL B-3977 (Beckman dextranase catalog N 680 000). Dextranase of bacterial origin can be obtained by adding alpha-1,3-dextran or a mixture of alpha-1,6, alpha-1,3-,

and alpha-1,4-dextrans.

  The bacterial strain can be inoculated into a

  shake bottle or in a fermentation device

  for a period of 1 to 5 days at 25-40 C. The sterile growth media can consist of dextran or

  of the mutane mentioned above, associated with a mixture of hy-

  carbon drate (starch, glucose, sucrose, cellulose), nitrogen compounds (protein digest, gelatin, casein, ammonium salts), growth stimulators, (yeast extract, corn maceration liquor, distillation residues ) or minerals. We can add

  conservation and decanting the enzyme, filtering it, or centering it

  triugate to precipitate cells (intracel dextranase-

  lular). Extracellular dextranase can be precipitated

  ammonium sulphate, acetone, sodium sulphate

  dium or a similar salt. Intracellular dextranases

  are autolyzed and extracted. After the fractionation stage

  by salt, we can further purify the enzyme by diff-

  different column chromatography methods (DEAE, Sephodex, ECTEOLA, hydroxyapatite) and freeze or stabilize it by adding protein, dextran, salt, etc. (The purification steps are usually done at

  low temperatures with refrigeration).

  The amount of dextranase used in the

  Oral Positions of the Invention is at least an effective amount to promote oral hygiene. This amount depends on the activity of the dextranase which can for example be between 1000 and 55,000 units per

  gram and therefore the method of preparation thereof. A My-

  dextranase enzyme material prepared in a ty-

  pique has an activity of about 5000 to 10,000 units per gram. One unit of Beckman dextranase is the amount of enzyme that produces 1 mole of reducing sugar from 1 mg of moltose monohydrate or native dextran per

minute at 35 C at a pH of 6.0.

  While smaller amounts of dextranase can be used, the dextranase having activity

  about 1000 to 55,000 units per gram can be pre-

  smells in amounts of about 0.05 to 4% by weight of the

composition for oral use.

  The toothpaste of the present invention preferably contains 5000 to 55,000 units per gram of toothpaste of initial enzymatic activity, it is stable from the physical and cosmetic point of view and it retains the activity of

  the enzyme over time (at least 75% of enzyme activity

  tick after 12 weeks of aging at 100 F

(37.8 C).

  An essential ingredient in toothpaste containing

  of the dextranase of the present invention is a tensio-

  compatible anionic active agent intended to form a stable foam and to help achieve a complete dispersion of

  composition in the oral cavity. Surfactants

  anionic active agents include a sulfonate, sulfate, carboxylate or phosphate as the anionic group

  conferring solubility in water. Examples of deter-

  suitable anionic gents include soaps, for

  example the water-soluble salts of higher fatty acids

  laughers or resin acids, those which can be pro-

  made from fats, oils and original waxes

  animal, vegetable or marine, for example soaps

  dium, greasy, coconut oil, tall oil and metals

  swaddles thereof; and sulfated and sulfonated synthetic detergents, especially those which have about 8 to 26 and preferably about 12 to 22 carbon atoms in the

  molecule. Examples of synthetic anionic detergents

  suitable include (higher) alkyl sulfonates

  aromatics with a single aromatic ring such as alkyl (su-

  lower) benzenesulfonates containing 8 to 16 carbon atoms

  bone in the alkyl group, in straight or branched chain,

  for example the sodium salt of decyl-, undecyl-, dodecyl-

  (lauryl), tridecyl-, tetradecyl-, pentadecyl- or hexadecyl-

  benzenesulfonate and (C8-C16) toluene-, xylene- and phenolsulfonate alkyls; alkyl (C8-C16) naphthalenesulfonate,

  ammonium diamylnaphthalenesulfonate and dinonylnaphtha-

  sodium lenesulfonate; sulfated aliphatic alcohols

  like sodium lauryl- and hexadecyl sulfates, lau-

  triethanolamine ryl sulfate and sodium oleyl sulfate; sulfated alcohol ethers, such as lauryl-, tridecyl- or

  tetradecyl sulfates having 1 to 5 ethylene oxide units

  lene; ammonium lauryl ether sulfate; Oils, acids

  sulfated and sulfonated fatty esters or esters, such as the sodium salts of sulfonated castor oil and oleic acid

  commercial sulfated; sulfated hydroxyamides such as hy-

  sulfated droxyethyllauramide; the sodium salt of lauryl-

  sulfoacetate; the sodium salt of dioctylsulfosuccinate,

  the sodium salt of oleylmethyltauride and N-lauroyl-

sodium sarcosinate.

  Also included in the scope of the invention are

  tion sulfuric esters of polyalcohols incompletely esterified by higher fatty acids, for example

  coconut oil monoglyceride monosulfate, monosul-

  ointment diglyceride fate; and hydroxy esters

  sulfonated higher fatty acids such as higher fatty acid esters of low weight alkylolsulfonic acids

  molecular, for example the oleic ester of iside acid

  thionic. The most common anionic surfactants

  used are ammonium, mono-, di- and tri-

  ethanolamine, and alkali metals (sodium and potassium) (higher) alkyl benzenesulfonates, higher alkylsulfates, fatty acid monoglyceride sulfates

  higher and sulfated ethoxylated alcohols, lauryl-

  ammonium ethersulfate, sodium N-lauroylsarcosinate,

  sodium dioctylsulfosuccinate and mixtures thereof

  this. It is preferred to use the anionic surfactant (s) in amounts of about 0.1 to 5% by weight per

  report to the support or excipient for dental use.

  Other suitable surfactants include

  non-ionic agents such as monostate cendensates

  sorbitan spleen with about 20 to 60 moles of d-ethyl oxide

  lene, ethylene oxide condensates with propylene glycol propylene oxide condensates ("Pluronics"); condensates of alcohol or higher fatty ethers with ethylene oxide; alkylthiophenol condensates with 15-unit ethylene oxide, and adducts of ethylene oxide to monoesters of hexalcohols and internal esters thereof such as sorbitan monolaurate, sorbitol monooleate , mannitan monopalmitate, and sorbitan monoisostearate. Specific examples include polyoxyethylene sorbitan monooleate

  (Tween 80), polyoxyethylene monoisostearate 20 is released

  bitan. It was found that a weighted ratio of the tensio-ac-

  anionic tif with non-ionic surfactant of 1/1, 1/2 and

  2/1 ensured good stability of dextranase in pre-

  sence of a polycationic stabilizer like Crotein Q. The amphoteric surfactant, which is a preferred additional surfactant, has both anionic and cationic groups, it is ionically balanced and its isoelectric point is at a pH of about 7; this group includes betaines and

  sulfobetaines. Betaines are a class of tensio-ac-

  amphoteric tifs which include alkylbétaines, alkylamidobétaines and alkylaminobétaines of general formula: R2

R -NR4-COO

  R3 in which R1 is an alkyl group having 10 to about

  carbon atoms, preferably 12 to 16 carbon atoms

bone, or an amido radical:

0 h

R-C-N- (CH2) a-

or an amino radical:

R-NH- (CH2) a-

  wherein R is an alkyl group having about 10 carbon atoms and a is an integer from 1 to 3; R2 and R3 are each alkyl groups having 1 to 3 carbon atoms and preferably 1 carbon atom; R4 is an alkylene or hydroxyalkylene group having 1 to 4 atoms

  carbon, and, optionally, a hydroxyl group. Sal-

  Typical kyldimethylbetaines include decylbetaine or 2- (N-decyl-N, N-dimethylammonio) acetate, cocobetaine

  or 2- (N-coco-N, N-dimethylammonio) acetate, myristylbe-

  tain, palmitylbétaine, laurylbétaïne, cetétbbe-

  tain, stearylbetaine, etc. Likewise, amidobetaines

  include cocoamidoethyl betaine, cocoamidopropyl-

  betaine, lauramidopropylbetaine and the like.

  The sulfobetaines, which are analogous in their structure to betaines, have sulfonate groups in place of the carboxylate groups, as represented by the general formula: R2

R -N-R4-SO

1, 4 3

  R3 in which R1, R2, R3 and R4 have the same meanings as above, and include alkylsulfobetaines,

  alkylamidosulfobétaines and alkylaminosulfobéta! nes.

  The molar ratio of anionic surfactant to

  amphoteric surfactant from 1 / 0.8 to 0.8 / 2 the optimum being from

  approximately 1.2 / 1, ensures good dextranase stability in the presence of a polycationic stabilizer such as Crotein Q.

  It is believed that the fact that this report is desired

  table is due to the optimum establishment of a mid-system

  that mixed helping to moderate the denaturing effect of lauryl-

  sodium sulfate, (LSS). If the quantity of betaine is increased to an LSS to betaine ratio of 1/2 or more, a stable toothpaste is obtained which has insufficient taste characteristics. This surfactant system forms

  a stable foam and helps achieve a consistent dispersion

  full of composition in the oral cavity.-J. Garcia Dominguez, International Journal of Cosmetic Science, vol. 3, 57-68 (1981) reveals that amphoteric betaine prevents sodium lauryl sulfate from denaturing dextranase in

  aqueous solution by forming mixed micelles by inter-

  ionic action with the LSS. However, his employment in a

  toothpaste together with the protein stabilizer ca

  ionic ensures an unexpected and unusual stability of the composition as well as the maintenance of the activity of

the aging enzyme.

  It is preferred to use the amphoteric surfactant in an amount of 2 to 6% by weight relative to the support or

* excipient for dental use.

  The inactivation of dextranase due to the presence of an anionic surfactant in the toothpaste makes it essential to add about 0.5 to 5% by weight of a

  cationic stabilizer with multi-positive charges

  ples, so that it reacts with the anionic surfactant sodium lauryl sulfate (LSS), thus reducing its ability

  to react with the enzyme dextranase. The stabilizers are

  required are polycationic and include proteins, polypeptides and polyamines. More specifically, the polycationic stabilizer is chosen from a derivative

  quaternary hydrolyzed collagen protein, polya

  lysine, polyarginine, protamine sulfate, a polyacryloxyalkylammonium salt, a polyvinylpyridinium salt

  polyoxyethylene (dimethyl amino) dichloroetha-

  ne (Busan-77), poly N- (2-hydroxypropyl) methacrylamide.

  Cationic surfactants do not play the role of

  dextranase stabilizer. The stable weight ratio

  reading / anionic surfactant is preferably in the range of 1/1 to 1/2 respectively, so that the stabilizer attaches to the anionic surfactant and prevents

its interaction with dextranase.

  The quaternary derivative of hydrolyzed collagen product, which is a preferred polycationic stabilizer, is a product of Croda Inc. of NewYork, known as Crotein Q, having a minimum pH of 9.5-10.5, is a powder dirty white fluid and its adopted name is steartrimonium hydrolyzed animal protein. The free amino groups in the protein molecule react with the quaternary ammonium reagent to form the quaternized derivative which has multiple positive charges. At pH below 5.5, Crotein Q has a double positive charge, due to the protonation of NH groups in the protein chain, as shown by a

  diagram in the Crodata 7778 brochure, page 2.

vNéCH3) N (CH3) X x '

\ ± NH2- - + N * C) NH

2 e2 CH2 x

N (CH3)

  At a pH of 9.5, the quaternary group of Cro-

  tein Q is always positively charged.

N (CH3) 3 N (CH3) 3

x x

X X

! I

NH NH

NR-x-N <+ H>) 3 NH x

N <CN3) 3

  Crotein Q has been evaluated as a stabilizer for dextranase activity in a mixed surfactant system. An aqueous system was used since the aging periods are shorter than those

  required for toothpaste. We calculated the times ne-

  so that the activity drops by 50% (t) and we

  use these times for comparison. You will find below

  under data representative of each study.

  Influence of the concentration of LSS on the activity of the enzyme: Concentration of LSS t - 26 C 0.5% 2.8 h 1.0% 0.8 h Inactivation of the enzyme increases with

concentration of LSS.

  Influence of temperature on enzyme inactivation: Experimental conditions t - 38 C t - 26 C 1% LSS O h 0.8 h 1% LSS + 1% Crotein 1.5 h 22.0 h 1% LSS + 0.5% Pluronic (mixed detergent) 1.1 h 14.6 h Mixed detergent + 1% Crotein 5.3 H 31.7 h The activity of the enzyme is stabilized by

low temperatures.

  Stability of the activity of the enzyme as a function of the concentration of Crotein Q: System t - 38 C 1% LSS, 1% Crotein 1.8 h 1% LSS, 2% Crotein 14.8 h In the presence of detergent ( s), the stability of the enzyme increases with the concentration of Crotein Q. Stability studies carried out on dextranase enzymes have shown that the inactivation effects of

  the enzyme of anionic surfactants such as lauryl-

  sodium sulfate (LSS), can be reduced in the presence of a polycationic stabilizer like Crotein Q. Although we do not know the exact mechanism by which the stabilizer plays this role, it is assumed that the polycationic stabilizer preferentially prevents the reaction of the enzyme with anionic surfactants without harming the

  foaming power of the surfactant. The preferred interaction

  stabilizer with surfactant prevents inactivity

  activation of dextranase by the anionic surfactant.

  The stability of toothpaste containing dex-

  tranase is measured by the maintenance of the activity of the

  zyme over an extended period, as shown in the table

  I .; in this table, comparative samples of this den-

  tifrice containing anionic and / or nonionic surfactants and stabilizers are evaluated by the following method. To 0.05 ml of a dilute dextranase solution in distilled water containing 200 to 300 units / ml, 0.95 ml of a dextran solution (average molecular weight) is added

  000) in 0.1 M sodium acetate and digested

  rer for 30 minutes at 37 C. At the end of the digestion period, 1 ml of colored reagent is added, with stirring,

  and it is heated in a boiling water bath for 15 minutes.

  The samples are cooled, 10 ml of water are added, with stirring, and the optical density readings are taken at 540 nm using maltose as a standard. Boil 1.0 ml of maltose solution (100-1000 pg / ml) and 1 ml of colored reagent for 15 minutes, cool and read optical density readings at 540 nm after addition of 10 ml of water. The colored reagent comprises 5 g of 3,5-dinitrosalicylic acid, 1 g of phenol,

  0.25 g of sodium sulfite and 100 g of potassium tartrate-

  sium and sodium dissolved in 500 ml of 2% NaOH. One unit of dextranase activity is defined as the activity which releases 1 pg of maltose monohydrate (Baker) per minute

at 37 C.

  Examples 1 to 7 inclusive are detailed below.

after.

TABLE I

  % of residual activity (Weeks at 38 C) Toothpastes 1 2 3 4 5 6 7 8 9 Control * 64.6 2.1 48.2-1.2 46.9 2.6 41.6 + 10.1 33 , 2 0.6 26.7i0.3 26.2 2.0 26.5 * 0.1 24.9 + 1, O (1) 0.5% LSS 4.8i0.2 3.5i0.3 5, 4i1.2 2.8 0.0 2.0i0.4 3.2, 0.3 * 1.6 * 0.3 2.0iO0.0 2.1 * 0.2 (2) 0.5% Pluronic ** 83.9 * 2.1 76.6 3.1 75, 7 3.1 62.1 * 1.8 56.9 2.1 49.5 2.2 46.5 * 1.3 51.3 + 2 , 8 47.1_0.1

(3) 0.5% LSS +

  0.5% Pluronic 68.4 * 1.9 55.3 + 1.6 43.7i1.0 32.7i1.7 28.7i0.4 21.50O, 6 18, 9i1.0 16.0i0.6 13 , 6 + 2.7

(4) 0.5% LSS +

  0.5% Pluronic + 1% Polyox *** 93.9 + 1.9 88.3 + 1.1 81.8 85 5.0 75.0 2.4 73.4-1.7 68.9 2.9 61.3 + 1.7 63.7 + 2.0 54.1 + 1.8

(5) 0.5% LSS +

  0.5% Pluronic 0 + 1% Crotein Q 94.8 + 0.8 95.2 + 1.1 92.6 * 3.2 98.2 1.9 94, 7 * 2.5 80.2i0.3 84.6 1.2 88.3 + 2.2 80.2 + 4.9

(6) 0.5% LSS +

  0.5% Pluronic + 1% Gelatin 76.5 + 1.1 78.2i0.5 71.1 + 0.9 75.2 * 4.7 74.4 * 1.9 66.9 + 2.3 64 .0 0.9 70.0 1.4 64.6 + 0.8

(7) 0.5% LSS +

0.5% Pluronic

+ 1% polyvi-

  nylpyrrolidone 70.7 * 0.6 55.8 1.8 49.2 + 1.9 48.1 3.5 38.5 + 0.3 34.9 * 1.7 32.9 * 1.0 30, 9 + 1.6 26.4 * 1.7

  * Control toothpaste containing enzyme but no LSS or stabilizer.

  ** Block copolymer of approximately 80% polyoxyethylene and approximately 20% polyoxypropylene, the latter radical having a molecular weight of 3250, obtained from BASF Wyandotte Co. *** Water-soluble resin, oxide oxide high molecular weight ethylene obtained from Union Carbide Corp. It is evident from the table that the enzyme itself, in the absence of detergent, is unstable in the dough

  toothpaste. Adding LSS increases the speed of

  activation (1). The addition of Pluronic to the LSS / enzyme toothpaste (3) does not stabilize dextranase. Adding Polyox in addition results in inadequate stabilization

  (4), in comparison with Crotein Q (5) which gives a better

  the stability of the enzyme in the toothpaste as gelatin

  tine (compare 5 and 6). Polyvinylpyrrolidone (7) does not

  not effectively bilize dextranase in toothpaste.

  The stability of toothpaste containing dex-

  tranase, measured by the maintenance of the activity of the enzyme

  over an extended period, is also presented in the ta-

  bleau II; in this table, comparative samples of this toothpaste containing anionic and amphoteric surfactants are evaluated by the manual method of Tsuchiya et al., Journal of Bacteriology, vol. 64 (4) 513-514,

  (1952), which can be modified for the Techni-

  con, the two methods using as a colorimetric reagent

  than dinitrosalicylic acid. Aged samples solubilized in water and in a buffer are incubated for 11.5 minutes with a dextran substrate,

  the reaction is stopped by boiling, the solution is reacted

  tion with the colored reagent, we bind the absorbance and we

  parers the results to a standard curve corresponding to the glu-

  cose to determine the percentage of enzyme activity

  residual. Despite aberrant results for the activi-

  enzymatic tees detected in some aged toothpaste samples, which show increasing activity

  with aging, the general trend highlights

  this a gradual decline in the activity of the enzyme of the dentit

  frice at the end of the 12 weeks of aging at 1000 F (37.8 C). In the case of some of the best formulas, we tested the keys and checked by analysis

256,485

  manual that there was a loss of up to 30% after 12 weeks when using the method described above.

  Examples 8 to 10 inclusive are detailed below.

  after. Examples 11 to 17 inclusive relate to other

  two-surfactant-stable toothpaste formulas

  health humectants, thickeners, classic flavorings

  and the like, in usual amounts as is ex-

  posed in the present description.

TABLE II

  LSS / betaine / dextranase formulas and aging results.

  Eg Ingredients Activity% of the initial activity of the enzyme

initial 3 se- 6 se- 9 se- 12 se-

  of the enzyme hand-held hand-held 8 3.6% dextranase (0.83 mg protein / ml), 2% Crotein Q, 1.8% LSS, 5% betaine, silica 53 130 104 98 103 99 9 3, 6% dextranase (0.83 mg protein / ml), 2% Crotein Q, 1.8% LSS, 5% betafne, alumina 49,392 100 98 87 79 0.65% dextranase (0.83 mg protein / ml ), 1% Crotein Q, 1.2% LSS, alumina 5 340 0 0 or 0 11 Alumina, 2% Crotein Q, 1.2% LSS, 4% betaine (l), 0.55% dextranase 4 570 110 101 95 83 12 Silica, 2% Crotein Q, 1.2% LSS, 4% betaine, 1% amide, 2.1% dextranase 18 600 84 71 67 81 13 Alumina, 2% Crotein Q, 1.2%

LSS, 4% betaine, 1.8% dex-

  tranase 13,800 99 88 107 107 14 Alumina, 2% Crotein Q, 0.3%

LSS, 0.9% ALES (2), 4% be-

  tafne, 1.8% dextranase 13,400 91 95 116 113 u Ln LnI TABLE II (continued) Ex. Ingredients Activity% of the initial activity of the enzyme

initial 3 se- 6 se- 9 se- 12 se-

  of the enzyme hand-held hand-held Alumina, 2% Crotein Q, 0.3% LSS, 0.9% ALES, 4% betaine, 3.6% dextranase 27 000 103 99 123 121 16 Silica, 2% Crotein Q, 0.3% LSS, 0.9% ALES, 4% betaine, 3.6% dextranase 28 300 102 99 120 119 17 Alumina, 3% Crotein Q, 1.2%

LSS, 5% betaine, 3.6% dex-

tranase 23 200 104 141 139 148

(1) Sulphated amido-betaine.

  (2) Laurylether ammonium sulfate.

  The samples were aged at 100 F (37.8 C). Activities determined by

  photocolorimetry using 3,5-dinitrosalicylic acid.

  Calculations: LSS (94% of active product, PM 288.38) 18 gx 0.94 = 16.9 g = 0.0587 moles Betaine (27% of active product, PM 283) 50 gx 0.27 = 13.5 g = 0.0477 moles

  LSS / betafne molar ratio = 0.06 / 0.05 = 1.2 / 1.

  o Ln The interaction of the stabilizer with the surfactant

  anionic intended to prevent its interaction with the dex-

  tranase does not significantly reduce the foaming power of

  the anionic agent. The foaming properties of surfactants

  assets are measured by the standardized method based on foam height. This analysis is carried out at 37 ° C. in distilled water and in hard water (105 ppm CaC12 and 70 ppm MgC12). The solutions are prepared so that they contain 0.1% LSS and 0.2% Crotein Q; separately or

  together. The volume of foam is measured in ml after 30

payments from the graduated cylinder.

TABLE III

  0.1% LSS 0.2% Crotein Q 0.1% LSS +

0.2% Cro-

  _ tein Q Distilled water 500 ml 12 ml 475 ml Hard water 238 ml 12 ml 425 ml These data show that the protein Q which is non-foaming does not easily decrease the foaming of LSS in distilled water and increases the foaming of LSS in hard water. We also note that the foaming of LSS in hard water is drastically reduced but is greatly increased by the stabilizer Crotein Q.

  The attached graph represents the relative volumes

  foam in distilled and hard water.

  These data show that in distilled water,

  the LSS produces the largest volume of foam.

  Combinations containing LSS and soft protein

  smells better than the combination of LSS and betaine, Crotein and betaine, and than betaine alone. We also note that Crotein does not easily reduce the foaming of LSS although betaine does. In hard water, the foaming of the LSS is drastically reduced, while the foaming of betaine is unchanged. In hard water, the foaming of the LSS is greatly increased by the Crotein stabilizer. Overall, these results indicate that the system that provides the best stabilization of the enzyme, the system

  LLSS + Crotein + betaine, has good characteristics

  foaming regardless of the hardness of the water. This

  point is important, because the concentration of di-

  valents in the oral cavity is very high.

  Accelerated aging studies show

  furthermore that enzy- stable toothpastes

  material, physical and cosmetic, approximate container-

  5000 units of dextranase per gram of toothpaste

  foam well in the presence of betaine, anio-surfactant

  cationic stabilizer as shown in the ta-

bleau IV.

TABLE IV

  Echan- Ingredients% of reten- Aged * Height tillon tion of tillon actinvideté at 100 F of foam __ enzymatic (37.8 OC) 1 Alumina 1.2% betaine, 1.2%

LSS, 2% Cro-

  tein Q 95 9 weeks 54 2 Alumina, 0.9% betaine, 0.8%

LSS, 1% Cro-

  tein Q 96 9 weeks 34 3 Alumina, 1.2% betaine, 0.6%

LSS, 1% Cro-

  tein Q 98 9 weeks 30 4 Silica, 1.2% beta, 0.6%

LSS, 2% Cro-

  tein Q 104 6 weeks 32 Alumina, 1.2% betaine, 0.6% LSS 83 9 weeks 35 6 Alumina, 1.2% betaine, 0.5% LSS 89 9 weeks 32 * The height of the foam is measured after shaking 1 g of toothpaste in 10 ml of hard water at 175 ppm (at 90 F,

32.2 C) for 15 seconds.

  Toothpastes and tooth powders

  usually contain a polishing agent or abra-

  sif practically insoluble in water which is compatible with the composition. Preferred compatible materials which do not harm the dentifrice composition include dicalcium phosphate dihydrate, silica and hydrated alumina. The polishing agent can be the only support material as in a tooth powder and is present in

  amount up to about 80% relative to the exci-

  suitable for dental use and generally around 30 to 75%

  compared to the excipient for dental use.

  In toothpaste formulas, liquids and solids must necessarily be dosed to form a creamy mass with the desired consistency, which can

  be extruded from a pressurized container or a tube

  before crashing (for example aluminum or lead).

  In general, the liquids in the toothpaste mainly contain water, glycerol, aqueous solutions of sorbitol, propylene glycol, polyethylene glycol 400, etc., and the appropriate mixtures thereof. It is advantageous

  usually use a mixture of water and a moist

  so much or a binder like glycerol or sorbitol. The total liquid content generally represents about 75% of the excipient. The amount of water represents

  generally about 10 to 25% of the excipient. We prefer em-

  also use a gelling agent in toothpaste

  such as natural and synthetic gums and substances

  those of the gum type such as carragheen, tragacanth, starch, sodium alginate, carboxymethylcellulose,

  Viscarine GMC, iota carrageenane and the like,

  in quantity of up to about 10%, and preferably from about 0.2 to 5% relative to the support or the excipient. The support may suitably contain a fluorinated compound having a beneficial effect on the care and hygiene of the oral cavity, for example a reduction

  of the solubility of enamel in acids and the protection

  tion of teeth against decay. Examples of these compounds

  These include stannous fluoride, potassium fluoride.

  sium and 6 t a i n, sodium hexafluorostannate, stannous chlorofluoride, sodium fluorozirconate and sodium monofluorophosphate. These substances, which dissociate or release fluorinated ions into the water, may be suitably present in the support

  in effective but non-toxic amounts, usually

  taken between about 0.1 and 5% by weight.

  Various other substances can also be incorporated into the support. Examples of these are coloring or whitening agents (e.g. titanium dioxide), preservatives (e.g.

  sodium benzoate), silicones, chloro-

  phylle, ammoniated substances like urea, phosphate

  diammonic and mixtures thereof, alcohol, men-

  thol and other constituents. These adjuvants are incorporated

  res to the compositions of the present invention in amounts

  which practically do not affect the properties and characteristics

  and are appropriately selected and used in appropriate amounts according to the particular type of

preparation in question.

  Flavoring or sweetening substances of the type commonly used in toothpaste may be

  incorporated into the support. These substances, if they are pre-

  help to modify the particular tastes of the ma-

  desired. Examples of these additives include

  flavoring oils, for example green mint oils

  te, peppermint, wintoreemn, sassafras, cloves, sage, eucalyptus, marjoram, cinnamon, lemon and orange, as well as methyl salicylate. The appropriate sweeteners

  include sucrose, lactose, maltose, sor-

  bitol, sodium cyclamate and saccharin. The aromati-

  health and the sweetener may appropriately represent

  ter together about 0.01 to 2% of the support.

  Toothpaste can be prepared by suitable mixing

  prayed ingredients. For example, to make a dough

  scent, we disperse a gelling agent such as an alginate

  sodium, carboxymethylcellulose or iota carra-

  geenane and a preservative such as sodium benzoate, if used, in a humectant such as glycerol. Water may also be present. Then we can

  mix additional humectant and water with the

  persion, which forms a homogeneous paste, gel or cream

  uncomfortable. The dental abrasive is then added, the

  surfactant and flavoring. The toothpaste is

  suite carefully de-aerated (for example under vacuum) and placed in a tube. The composition can be deaerated during the

mixing or after mixing.

  The following examples only illustrate the

  vention, but it is understood that the invention is not

  not limit to these examples. All the quantities of the differ-

  rents ingredients are by weight, unless otherwise indicated.

Examples 1 to 7.

  The stabilization properties of Examples 1 to

  7, which are measured by the maintenance of the activity of the

  zyme for a certain period and recorded in the ta-

  bleau I, clearly show the superiority of the composition

  tion of Example 5 which contains a polycatio stabilizer

  nique in an anionic surfactant dextranase toothpaste according to the present invention. the other examples concern

  control compositions which do not contain stabilizers

  polycationic health (1) or in which the polycationic stabilizer has been replaced by nonionic surfactants (3 and 4) or by cationic compounds (7) or

with gelatin (6).

Toothpaste Ingredients%

(1) (2) (3) (4) (5) (6) (7)

  Glycerol 15 15 15 15 15 15 15 Sodium alginate 1.0 1.0 1.0 1.0 1.0 1.0 1.0 Sorbitol solution

70% 15 15 15 15 15 15 15

  Saccharin sodium salt 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1

LSS 0.5 - 0.5 0.5 0.5 0.5 0.5

  Pluronic (F108) 1 - 0.5 0.5 0.5 0.5 0.5 0.5 Hydrated alumina 50 50 50 50 50 50 50 Silica Huber 2 2 2 2 2 2 2 Flavoring 1 1 1 1 1 1 1 Dextranase 5000 5000 5000 5000 5000 5000 5000

uni- uni-

tees / g tees / g

Crotein Q - - - - 1.0 - -

Gelatin - - - - - 1.0

Polyox - - - 1, O - - -

Polyvinylpyrro-

  lidone - - - - - - 1.0 Water the remainder 1 Block copolymer of approximately 80% polyoxyethylene and approximately 20% polyoxypropylene, the latter radical having a molecular weight of 3250, obtained from BASF Wyandotte Co. 2 Resin soluble in water, high molecular weight polymer of ethylene oxide, obtained from Union Carbide Corp.

Example 8.

Gel toothpaste.

* Ingredients Glycerol 25

CMC1 0.35

  Sodium benzoate 0.5 Saccharin sodium salt 0.2 Monofluorophosphate (MFP) 0.76 Sorbitol 24.09 Carbowax - PEG 6002 3 Blue FDC N 1 1% 0.1 Deionized water 6.6 Silica containing combined alumina3 18 Colloidal silica airgel4 8 Sodium lauryl sulfate (LSS) 1.8 Betaine tego-S-10665 5 Crotein Q 2 Flavoring 1 Dextranase (20,000 units / g) 3.6 pH 6.5 Foam 84 ml 1Carboxymethylcellulose 2Polyethylene glycol, molecular weight 600 3Zeo 49B, from JM Huber 4Syloid 244 Sulfated amphoteric surfactant manufactured by Goldschmidt

  Chemical Corp., active ingredient content 27%.

Example 9.

Toothpaste.

  Ingredients% Iota carrageenane 0.9 Glycerol 22 Sodium benzoate 0.5 Saccharin sodium salt 0.2

MFP 0.76

  Deionized water 12.24 Hydrated alumina 50

-LSS 1.8

  Amidobetaine sulfated (1.5% active product) 5 Crotein Q 2 Flavoring 1 Dextranase (20,000 units / g) 3.6 pH 7.41 Foam 83 ml

Example 10.

Toothpaste.

  Ingredients% Iota carrageenane 0.9 Glycerol 22 Saccharin sodium salt 0.2 Methyl parahydroxybenzoate 0.1

MFP 0.76

  Crotein Q 1 Deionized water 23.19 Hydrated alumina 50

LSS 1.2

  Dextranase (20,000 units / g) 0.65 pH 8.08 Removal of betaine reduces stability

  of the enzyme of this composition to zero.

Example 11.

Toothpaste.

  Ingredients% Iota carrageenane 0.9 Glycerol 22

MFP 0.76

  Sodium benzoate 0.5 Saccharin sodium salt 0.2 Hydrated alumina 50

LSS 1.2

  Betaine 4.0 Crotein Q 2 Flavoring 1 Dextranase (10,000 units / g) 1.8 Deionized water 15.64 pH 7.44 Foam 58 ml

Example 12.

Toothpaste.

  Ingredients% Iota carrageenane 0.9 Glycerol 22 Sodium benzoate 0.5 Saccharin sodium salt 0.2

MFP 0.76

  Hydrated alumina 50 Deionized water 13.64

LSS 1.2

  Betaine teg-S 1066 5 Crotein Q 3 Flavoring 1 Dextranase (10,000 units / g) 1.8 pH 7.17 Foam 71 ml

Example 13.

Toothpaste.

  Ingredients% Iota carrageenane 0.9 Glycerol 22 Sodium benzoate 0.5 Saccharin sodium salt 0.2

MFP 0.76

  Hydrated alumina 50 Deionized water 11.84

LSS 1.2

  Betaine 5 Crotein Q 3 Flavoring 1 Dextranase (20,000 units / g) 3.6 pH 7, 28 Foam 60 ml

Example 14.

Mouthwash.

  Ingredients% Ethanol (90%) 5-10 Glycerol 10-20 Saccharin sodium salt 0-5 Sodium benzoate 0-5 Pluronic F108 1-2 Dextranase (1,000 to 20,000 units / ml) 0.05-1 Sodium lauryl sulfate (LSS) 0.1-0.5 Crotein Q 0.5-3 Flavoring 0.22-0.5 Water the rest

Example 15.

Tooth powder.

  Ingredients% Hydrated alumina 70-80 Glycerol 10

LSS 0.1-0.5

  Saccharin sodium salt 0.1 Flavoring 1.0 Dextranase (1,000 to 20,000 units / g) 0.05-1.0 Crotein Q 0.5-3 Water the rest

Example 16.

  Chewing gum. Ingredients% Gum base, e.g. gum

  arabic, containing a charge of elas-

  natural or synthetic seed 20-35 Sorbitol 10-20 Dextranase (5,000 to 20,000 units / g) 0.1-1.0 Sodium lauryl sulfate 0.1-0.5 Crotein Q 0.5-3 Flavoring 0.5 -2 Dextrose the rest

Example 17.

Toothpaste.

  Ingredients% Glycerol 22 Carrageenane 1,3 Dicalcium phosphate dihydrate 50 Sodium benzoate 0.5 Saccharin sodium salt 0.2 Crotein Q 1.3

LSS 0.75

  Pluronic F108 0.5 Flavoring 1 Dextranase (5,000 to 20,000 units / g) 0.051.0 Water the rest The following table defines other toothpaste compositions containing dextranase which are stable from the physical and cosmetic point of view and which keep

  enzyme activity in aging.

  - The compositions of Examples 18 to 26 inclusive comprise an aqueous excipient containing a suitable flavoring agent. The compositions of Examples 18 to 21 inclusive

  contain 1.8% dextranase. The composition of the example-

  ple 22 contains 2.1%, that of example 23 2.5% and

  those of Examples 24 to 26 included 3.6% dextranase.

  Conventional humectants or mixtures thereof are

  used in appropriate quantities as defined

  finished in this patent text. The compositions of the examples

  ples 18, 19, 21, 24 and 26 include glycerol, while those of examples 20, 22, 23 and 25 include a

  mixture of glycerol and sorbitol.

EXAMPLES 10 to 18

Toothpaste

  Eg Ingredients Foam Activity ini- Activity at% of ac-

  (ml) tial of the end of 12 zyme activity (units) weeks to initial

O F (37.8 C)

  18 Iota * / alumina, 1.2% LSS, 4% betaine, 2% Crotein Q 58 13 800 15 200 110 19 Iota / alumina, 3% LSS, 0.9% ALES, 4% betaine, 2% Crotein Q 58 13,400 15,100 113 Silica, 0.3% LSS, 0.9% ALES, 4% betaine, 2% Crotein Q 46 14,000 15,800 113 21 Iota / alumina, 1.2% LSS, w% betaine, 3% Crotein Q 71 12 500 16 100 129 '22 Silica, 1.2 LSS, 4% betaine, 2% Crotein Q, 1% amide 55 18 600 15 000 81 23 Silica, 0.6% LSS, 4% betaine, 2% Crotein Q. 1% amide 37 23 800 18 700 79 24 Iota / alumina, 0.3% LSS, 0.9% ALES, 4% betaine, 2% Crotein Q 50 27 000 32 700 121 Silica, 0.3% LSS , 0.9% ALES, N 4% betaine, 2% Crotein Q 51 28 300 33 800 119 O Lni 26 Iota / alumina, 1.2% LSS, 5% betaine, 3% Crotein Q 60 23 200 34 300 148

* Carrageenane.

  You can make changes in the components

  above. For example, you can replace the ten-

  specific surfactants of the examples by other surfactants -

  anionic active agents such as (higher) alkyl benzenesulfo-

  nates, fatty acid soaps such as tallow soap,

  sulfated alcohol ethers and the like. Likewise, we can

  place specific betaine surfactants with other betaines and sulfated betaines. Likewise, we can replace

  specific nonionic surfactants in examples

  ples by other nonionic surfactants.

  Carboxymethylcellulose, carrageenan or sodium alginate can be replaced by other thickening or gelling agents such as starch, carragheen,

tragacanth and the like.

  Likewise, the Crotein Q used in the examples can be replaced by other polycationic stabilizers.

  such as polylysine, polyarginine, prota- sulfate

  mine, polyvinylpyridinium salts, poly N-

(2-hydroxypropyl) methacrylamide.

  It is understood that the detailed description

  above is for illustration purposes only

  and that changes can be made to it without delay

  gain from the spirit of the invention. The descriptive abstract given above is for the convenience of technical researchers only and should not be given any weight in this regard.

  which relates to the scope of the invention.

Claims (20)

  1. Foaming stable composition for buccal use comprising one or more anionic surfactants, a   dextranase in a quantity that can provide 1,000 to 55,000 units   teas / g of initial enzymatic activity, and a polycationic stabilizer in an amount that can effectively stabilize   the activity of the enzyme against decline during old age   smoothing, and against inactivation due to the presence of anionic surfactants chosen from proteins, polypeptides and polyamines, in an anionic surfactant to stabilizer weight ratio of 1/1 to 1/2, in an excipient for dental use.   2. Composition for oral use according to the claim   tion 1, characterized in that the polycation stabilizer   that is chosen from a quaternary derivative of hydrolyzed collagen protein, polylysine, polyarginine, protamine sulfate, a polyacryloxyalkylammonium salt,   polyvinylpyridinium salts, polyoxyethyl   lene (dimethylamino) dichloroethane, poly-N- (2-hydroxy- propyl) methacrylamide.   3. Composition for oral use according to the claim   tion 1, containing a nonionic surfactant in a report   weight bearing non-ionic surfactant on surfactant anionic from 2/1 to 1/2.   4. Composition for oral use according to the claim   tion 1, containing an amphoteric surfactant chosen from betaines and sulfobetaines, in a molar ratio of anionic surfactant to amphoteric surfactant of 1 / 0.8 at 0.8 / 2.   5. Composition for oral use according to the claim   tion 1, characterized in that the amphoteric surfactant has the general formula: R2 Rl-N ± R4-COo or 1.4 R3 R2 R1-N ± R4-S3   l * 1 4 3 R3 o R is an alkyl group having 10 to 20 carbon atoms, the amido radical: OH il I R-C-N- (CH2) a- or the amino radical: R-NH- (CH2) a-   o R is an alkyl radical having 10 to 20 carbon atoms and a is an integer from 1 to 3; R2 and R3 are each alkyl radicals having 1 to 3 carbon atoms; and R4   is an alkylene or hydroxyalkylene group having 1 to 4 ato-   carbon, the amphoteric surfactant constituting about   ron 2 to 6% by weight of the composition.   6. Composition for oral use according to the claim   tion 1, characterized in that the polycation stabilizer   that constitutes about 0.5 to 5% by weight of the composition.   7. Composition for oral use according to the claim   tion 1, characterized in that dextranase represents   about 0.05 to 4% by weight of the composition.   8. Composition for oral use according to the claim   tion 1, characterized in that the anionic surfactant is sodium lauryl sulfate.   9. Composition for oral use according to the claim   tion 1, characterized in that the anionic surfactants are sodium lauryl sulfate and ammonium lauryl ether sulfate.   10. Composition for oral use as claimed   cation 3, characterized in that the non-ionic surfactant   that is a block copolymer consisting of approximately 80% polyoxyethylene and approximately 20% polyoxypropylene having a molecular weight of 3,250.   11. Composition for oral use according to the claim   cation 1, in the form of a toothpaste, containing   about 30 to 75% by weight of an insoluble polishing agent ble in water.   12. Composition for oral use according to the claim   cation 1, in the form of a tooth powder, containing approximately 70 to 80% of a polishing agent insoluble in 1 water.   13. Composition for oral use according to the claim   cation 1, in the form of an aqueous mouthwash containing about 5-10% ethanol.   14. Composition for oral use according to the claim   cation 1, in the form of chewing gum, comprising approxi-   20 to 35% of a gum base containing a filler   natural or synthetic elastomer.   15. Composition for oral use according to the claim   cation 11, characterized in that the polishing agent is hydrated alumina.   16. Composition for oral use as claimed   cation 11, characterized in that the polishing agent is dicalcium phosphate dihydrate.   17. Composition for oral use as claimed   cation 11, characterized in that the polishing agent is silica.   18. Composition for oral use as claimed   cation 11, having a liquid content of 20 to 75% per relative to the weight of the composition.   19. Composition for oral use according to the claim   cation 4, characterized in that the polycatio-   nique is a quaternary derivative of hydrolyzed collagen protein.   20. Composition for oral use as claimed   cation 11, characterized in that the polycatio-   nique is a quaternary derivative of hydrolyzed collagen protein.