FR2420764A1 - Procede et reactif pour le dosage de l'heparine bio-active dans le plasma - Google Patents

Procede et reactif pour le dosage de l'heparine bio-active dans le plasma

Info

Publication number
FR2420764A1
FR2420764A1 FR7907424A FR7907424A FR2420764A1 FR 2420764 A1 FR2420764 A1 FR 2420764A1 FR 7907424 A FR7907424 A FR 7907424A FR 7907424 A FR7907424 A FR 7907424A FR 2420764 A1 FR2420764 A1 FR 2420764A1
Authority
FR
France
Prior art keywords
plasma
reagent
determination
bio
measurement
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
FR7907424A
Other languages
English (en)
Other versions
FR2420764B1 (fr
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim GmbH filed Critical Boehringer Mannheim GmbH
Publication of FR2420764A1 publication Critical patent/FR2420764A1/fr
Application granted granted Critical
Publication of FR2420764B1 publication Critical patent/FR2420764B1/fr
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/56—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving blood clotting factors, e.g. involving thrombin, thromboplastin, fibrinogen
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2337/00—N-linked chromogens for determinations of peptidases and proteinases
    • C12Q2337/10—Anilides
    • C12Q2337/12—Para-Nitroanilides p-NA
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/81—Protease inhibitors
    • G01N2333/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
    • G01N2333/811—Serine protease (E.C. 3.4.21) inhibitors
    • G01N2333/8114—Kunitz type inhibitors
    • G01N2333/8117—Bovine/basic pancreatic trypsin inhibitor (BPTI, aprotinin)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/95—Proteinases, i.e. endopeptidases (3.4.21-3.4.99)
    • G01N2333/964—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue
    • G01N2333/96425—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals
    • G01N2333/96427—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general
    • G01N2333/9643—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general with EC number
    • G01N2333/96433—Serine endopeptidases (3.4.21)
    • G01N2333/96441—Serine endopeptidases (3.4.21) with definite EC number
    • G01N2333/96444—Factor X (3.4.21.6)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/974—Thrombin
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2400/00—Assays, e.g. immunoassays or enzyme assays, involving carbohydrates
    • G01N2400/10—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
    • G01N2400/38—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence, e.g. gluco- or galactomannans, e.g. Konjac gum, Locust bean gum, Guar gum
    • G01N2400/40—Glycosaminoglycans, i.e. GAG or mucopolysaccharides, e.g. chondroitin sulfate, dermatan sulfate, hyaluronic acid, heparin, heparan sulfate, and related sulfated polysaccharides
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
    • Y10T436/108331—Preservative, buffer, anticoagulant or diluent
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Neurosurgery (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Hematology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Procédé et réactif pour la détermination de l'activité biologique de l'héparine dans le plasma par addition de thrombine ou du facteur Xa en tant qu'enzyme protéolytique et d'un substrat chromogène de cette dernière et dosage du colorant libéré à partir du substrat chromogène. La mesure est effectuée sans adjonction d'antithrombine III. Le procédé selon l'invention permet une mesure indépendante de la quantité d'antithrombine III contenue dans le plasma soumis à la mesure.
FR7907424A 1978-03-23 1979-03-23 Procede et reactif pour le dosage de l'heparine bio-active dans le plasma Granted FR2420764A1 (fr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE2812943A DE2812943C3 (de) 1978-03-23 1978-03-23 Verfahren und Reagens zur Bestimmung der biologischen Aktivität von Heparin im Plasma

Publications (2)

Publication Number Publication Date
FR2420764A1 true FR2420764A1 (fr) 1979-10-19
FR2420764B1 FR2420764B1 (fr) 1983-07-29

Family

ID=6035376

Family Applications (1)

Application Number Title Priority Date Filing Date
FR7907424A Granted FR2420764A1 (fr) 1978-03-23 1979-03-23 Procede et reactif pour le dosage de l'heparine bio-active dans le plasma

Country Status (22)

Country Link
US (1) US4234682A (fr)
EP (1) EP0004271A3 (fr)
JP (1) JPS54128796A (fr)
AR (1) AR217503A1 (fr)
AT (1) AT362080B (fr)
AU (1) AU512206B2 (fr)
BE (1) BE875014A (fr)
CA (1) CA1111334A (fr)
CH (1) CH642749A5 (fr)
DD (1) DD141952A5 (fr)
DE (1) DE2812943C3 (fr)
DK (1) DK149475C (fr)
FI (1) FI62859C (fr)
FR (1) FR2420764A1 (fr)
GB (1) GB2017298B (fr)
HU (1) HU176068B (fr)
IE (1) IE48067B1 (fr)
IT (1) IT1112966B (fr)
LU (1) LU81070A1 (fr)
NL (1) NL7902259A (fr)
SE (1) SE445560B (fr)
ZA (1) ZA79622B (fr)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2903701C2 (de) * 1979-01-31 1980-10-16 Boehringer Mannheim Gmbh, 6800 Mannheim Kontrollreagens für die Bestimmung der Heparinaktivität
US4244865A (en) * 1979-12-03 1981-01-13 Abbott Laboratories α hydroxy tripeptide substrates
DE3005540A1 (de) * 1980-02-14 1981-08-20 Boehringer Mannheim Gmbh, 6800 Mannheim Verfahren und reagens zur bestimmung der biologischen aktivitaet von heparin im plasma
DE3019612A1 (de) * 1980-05-22 1981-11-26 Boehringer Mannheim Gmbh, 6800 Mannheim Stabilisiertes thrombinpraeparat
EP0049877B1 (fr) * 1980-10-09 1984-05-23 Roche Diagnostics GmbH Procédé et réactif pour la détermination de l'antithrombine-BM
US4473639A (en) * 1982-09-15 1984-09-25 Miles Laboratories, Inc. Reagent strip test for antithrombin-III
US4543335A (en) * 1982-12-20 1985-09-24 Miles Laboratories, Inc. Device and method for the quantitative determination of heparin in mammalian blood plasma
US4948724A (en) * 1985-09-05 1990-08-14 Yin E Thye Composition, kit and method for assaying heparin and a method for making the composition
US4851336A (en) * 1985-09-05 1989-07-25 Yin E Thye Composition, kit, and method for assaying heparinano a method for making the composition
ATE64470T1 (de) * 1985-09-05 1991-06-15 E Thye Yin Verfahren und zusammensetzungen zur bestimmung von heparin.
US4946775A (en) * 1985-09-05 1990-08-07 Yin E Thye Composition, kit and method for assaying heparin and a method for making the composition
US5939325A (en) * 1989-07-20 1999-08-17 Analytical Control Systems, Inc. Stable whole blood coagulation controls
EP0482088B1 (fr) * 1989-07-20 1999-03-31 Analytical Control Systems, Inc. Regulations ameliorees pour effectuer une coagulation stable du sang
EP0509086B1 (fr) * 1990-11-05 1995-04-12 Dade Produktions AG Procede de determination de la concentration d'anticoagulants
US5308755A (en) * 1992-06-08 1994-05-03 Research Corporation Technologies, Inc. Method for measuring heparin
EP1325962A1 (fr) * 2001-12-17 2003-07-09 Gentium S.p.A. Procédé pour la détermination de l'activité biologique de la defibrotide
WO2005111231A2 (fr) * 2004-05-11 2005-11-24 Heptest Laboratories, Inc. Compositions, kit et procede en une etape permettant de surveiller des composes possedant des activites d'antifacteur xa et/ou d'antifacteur iia
US7219827B2 (en) 2004-11-19 2007-05-22 General Electric Company Braze end isolation layer for generator armature winding bar and method for applying the isolation layer
DE102005038418A1 (de) * 2005-08-12 2007-02-15 Dade Behring Marburg Gmbh Faktor Xa-basierter Heparinassay unter Verwendung einer Heparin-modifizierenden Komponente
DK2637672T3 (en) 2010-11-12 2018-10-22 Gentium S R L DEFIBROTID FOR USE IN PROPHYLAXY AND / OR TREATMENT OF GRAPHIC VERSUS HOST DISEASE (GVHD)
EP2484775A1 (fr) 2011-02-07 2012-08-08 Siemens Healthcare Diagnostics Products GmbH Procédé non sensible à l'héparine destiné à la détermination d'inhibiteurs de facteurs de coagulation directs
ES2660969T5 (es) 2012-06-22 2021-09-03 Gentium S R L Método basado en euglobulina para determinar la actividad biológica de defibrotida
EP2843416A1 (fr) 2013-09-02 2015-03-04 Siemens Healthcare Diagnostics Products GmbH Matériau de contrôle et de calibrage pour la détermination d'anticoagulants directs
EP3026122A1 (fr) 2014-11-27 2016-06-01 Gentium S.p.A. Procédé à base cellulaire pour déterminer la puissance de défibrotide
EP3348649A1 (fr) 2017-01-16 2018-07-18 Siemens Healthcare Diagnostics Products GmbH Matériel de contrôle universel pour tests d'inhibiteur de facteur de coagulation

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2294390A1 (fr) * 1974-12-10 1976-07-09 Battelle Memorial Institute Dispositif projecteur a polarisation
FR2317280A1 (fr) * 1975-07-11 1977-02-04 Kabi Ab Nouveaux substrats chromogenes pour la thrombine
DE2708985A1 (de) * 1976-03-08 1977-09-29 Leuven Res & Dev Vzw Ermittlung von haeparin in blutplasma
US4067777A (en) * 1976-05-13 1978-01-10 Innerfield Irving Determination of heparin in the blood

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3769617A (en) * 1971-12-09 1973-10-30 Rca Corp Transmission line using a pair of staggered broad metal strips
SE380257B (sv) * 1972-05-02 1975-11-03 Bofors Ab Nya diagnostiskt verksamma substrat med hog specificitet till trombin och andra proteolytiska enzymer av typen peptidyl-peptid-hydrolaser
US4162941A (en) * 1974-12-05 1979-07-31 Ab Kabi Method for determining a proteolytic enzyme
CH622286A5 (fr) * 1975-06-23 1981-03-31 Pentapharm Ag
US4061625A (en) * 1975-07-11 1977-12-06 Ab Kabi Novel chromogenic thrombin substrates
DE2601372C3 (de) * 1976-01-15 1980-03-27 Behringwerke Ag, 3550 Marburg Verfahren zur Bestimmung von Antithrombin III

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2294390A1 (fr) * 1974-12-10 1976-07-09 Battelle Memorial Institute Dispositif projecteur a polarisation
FR2317280A1 (fr) * 1975-07-11 1977-02-04 Kabi Ab Nouveaux substrats chromogenes pour la thrombine
DE2708985A1 (de) * 1976-03-08 1977-09-29 Leuven Res & Dev Vzw Ermittlung von haeparin in blutplasma
US4067777A (en) * 1976-05-13 1978-01-10 Innerfield Irving Determination of heparin in the blood

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CA1977 *
CA1979 *
EXBK/76 *

Also Published As

Publication number Publication date
IT7921220A0 (it) 1979-03-22
FI62859C (fi) 1983-03-10
AR217503A1 (es) 1980-03-31
DK149475C (da) 1986-12-01
BE875014A (fr) 1979-09-24
CH642749A5 (de) 1984-04-30
GB2017298A (en) 1979-10-03
DK149475B (da) 1986-06-23
FR2420764B1 (fr) 1983-07-29
DD141952A5 (de) 1980-05-28
GB2017298B (en) 1982-07-07
EP0004271A3 (fr) 1980-04-02
DE2812943B2 (de) 1980-08-14
LU81070A1 (de) 1979-06-19
HU176068B (en) 1980-12-28
NL7902259A (nl) 1979-09-25
FI790476A (fi) 1979-09-24
ATA32179A (de) 1980-09-15
AU512206B2 (en) 1980-09-25
US4234682A (en) 1980-11-18
SE7902555L (sv) 1979-09-24
FI62859B (fi) 1982-11-30
DE2812943C3 (de) 1981-05-14
ZA79622B (en) 1980-03-26
IE48067B1 (en) 1984-09-19
SE445560B (sv) 1986-06-30
CA1111334A (fr) 1981-10-27
DK57079A (da) 1979-09-24
IE790238L (en) 1979-09-23
DE2812943A1 (de) 1979-10-04
JPS5726120B2 (fr) 1982-06-02
IT1112966B (it) 1986-01-20
JPS54128796A (en) 1979-10-05
AU4382579A (en) 1979-09-27
EP0004271A2 (fr) 1979-10-03
AT362080B (de) 1981-04-27

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