FR2393306A1 - Procede et appareillage pour la determination en continu de la concentration d'un substrat d'enzyme - Google Patents

Procede et appareillage pour la determination en continu de la concentration d'un substrat d'enzyme

Info

Publication number
FR2393306A1
FR2393306A1 FR7728298A FR7728298A FR2393306A1 FR 2393306 A1 FR2393306 A1 FR 2393306A1 FR 7728298 A FR7728298 A FR 7728298A FR 7728298 A FR7728298 A FR 7728298A FR 2393306 A1 FR2393306 A1 FR 2393306A1
Authority
FR
France
Prior art keywords
substrate
concentration
continuous determination
enzyme substrate
production
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
FR7728298A
Other languages
English (en)
Other versions
FR2393306B1 (fr
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim GmbH filed Critical Boehringer Mannheim GmbH
Publication of FR2393306A1 publication Critical patent/FR2393306A1/fr
Application granted granted Critical
Publication of FR2393306B1 publication Critical patent/FR2393306B1/fr
Granted legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G01N27/3271—Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)

Abstract

L'invention a pour objet un procédé et un appareillage pour la détermination en continu de la concentration d'un substrat d'enzyme dans un liquide aqueux. Selon ce procédé on introduit un échantillon contenant ce substrat dans un courant de solution tampon, on convertit le substrat sur une enzyme immobilisée et on mesure physiquement une modification résultante de la solution dans une chambre de mesure. On envoie alternativement le courant de solution tampon contenant l'échantillon à analyser directement dans la chambre de mesure, avec production d'un signal de référence puis d'abord sur l'enzyme immobilisée et ensuite dans la chambre de mesure, avec production d'un signal de mesure. Le procédé et l'appareillage selon l'invention permettent de corriger les résultats d'analyse de certaines influences gênantes.
FR7728298A 1976-09-20 1977-09-20 Procede et appareillage pour la determination en continu de la concentration d'un substrat d'enzyme Granted FR2393306A1 (fr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE2642232A DE2642232C3 (de) 1976-09-20 1976-09-20 Verfahren und Vorrichtung zur laufenden Bestimmung der Konzentration eines Enzymsubstrates

Publications (2)

Publication Number Publication Date
FR2393306A1 true FR2393306A1 (fr) 1978-12-29
FR2393306B1 FR2393306B1 (fr) 1984-05-11

Family

ID=5988351

Family Applications (1)

Application Number Title Priority Date Filing Date
FR7728298A Granted FR2393306A1 (fr) 1976-09-20 1977-09-20 Procede et appareillage pour la determination en continu de la concentration d'un substrat d'enzyme

Country Status (15)

Country Link
US (1) US4153513A (fr)
JP (1) JPS5339198A (fr)
AT (1) AT352293B (fr)
BE (1) BE858791A (fr)
CA (1) CA1086616A (fr)
CH (1) CH634108A5 (fr)
DD (1) DD132150A5 (fr)
DE (1) DE2642232C3 (fr)
DK (1) DK376677A (fr)
FR (1) FR2393306A1 (fr)
GB (1) GB1530847A (fr)
HU (1) HU180210B (fr)
IT (1) IT1080795B (fr)
NL (1) NL7707199A (fr)
SE (1) SE7709357L (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2372432A1 (fr) * 1976-11-30 1978-06-23 Gambro Ab Procede et dispositif pour mesurer la concentration d'un compose de faible poids moleculaire dans un milieu complexe

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE442640B (sv) * 1977-12-16 1986-01-20 Gambro Lundia Ab Sett och anordning for att meta koncentrationen av en forening i ett flytande medium
JPS55141246A (en) * 1979-04-24 1980-11-05 Zenshirou Suzuki Odor reducing agent and its preparation
DE3273657D1 (en) * 1981-04-24 1986-11-13 Kyoto Daiichi Kagaku Kk A device for automatically and continuously measuring the constituent parts of blood
SE453754B (sv) * 1982-06-15 1988-02-29 Gambro Lundia Ab Anordning for metning av koncentrationen av en lagmolekyler forening i ett komplext medium
US4837157A (en) * 1982-07-20 1989-06-06 Coventry Health Authority Sample preparation method for liquid chromatography
CS238261B1 (en) * 1983-07-28 1985-11-13 Jiri Cerkasov Enzymatic reactions execution and tracing method and device for application of this method
AT382885B (de) * 1984-04-12 1987-04-27 Biochemie Gmbh Verfahren zur enzymkatalysierten konzentrations- bestimmung von substraten
US5158868A (en) * 1987-07-17 1992-10-27 Iniziative Marittime 1991, S.R.L. Method of sample analysis
US4997627A (en) * 1987-07-17 1991-03-05 Fisher Scientific Company Sample analysis
US5206145A (en) * 1988-05-19 1993-04-27 Thorn Emi Plc Method of measuring the concentration of a substance in a sample solution
US5037737A (en) * 1988-06-29 1991-08-06 Apec, Inc. Analysis by sensor placement in recprocating flow
US5133937A (en) * 1989-06-01 1992-07-28 Iniziative Marittime, 1991 S.R.L. Analysis system having a removable reaction cartridge and temperature control
FR2679334B1 (fr) * 1991-07-19 1997-06-27 Ciposa Microtech Procede pour recueillir un echantillon de dialysat et dispositif pour la mise en óoeuvre de ce procede.
DE4214822C2 (de) * 1992-05-05 1998-12-03 Inst Bioanalytik Gmbh Verfahren und Vorrichtung zur kontinuierlichen Bestimmung von Substraten von Oxidoreduktasen
BR9508456A (pt) * 1994-07-29 1997-12-23 Gambro Ab Processo e dispositivo para medir a concentração de uréia ou de uma subst ncia similar em uma solução compósita
SE9702658D0 (sv) 1997-07-09 1997-07-09 Thomas Carlsson Regeneration of biosensors
WO2003048382A1 (fr) * 2001-12-03 2003-06-12 Sievers Instruments, Inc. Detection sensible d'uree et de composes associes dans de l'eau
CN112763482B (zh) * 2021-02-03 2022-08-09 苏州长光华医生物医学工程有限公司 一种底物液供液装置

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3512517A (en) * 1964-11-30 1970-05-19 Beckman Instruments Inc Polarographic method and apparatus for monitoring blood glucose concentration
FR2142416A5 (fr) * 1971-06-18 1973-01-26 Boehringer Mannheim Gmbh
US3919051A (en) * 1974-07-11 1975-11-11 Honeywell Inc Biological analyzer and method
US3930957A (en) * 1974-07-11 1976-01-06 Honeywell Inc. Apparatus and method for biological analysis
US3937615A (en) * 1974-12-17 1976-02-10 Leeds & Northrup Company Auto-ranging glucose measuring system

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3915804A (en) * 1974-07-15 1975-10-28 Corning Glass Works Apparatus and method for measuring conductivity change in a urea-urease reaction
US4067777A (en) * 1976-05-13 1978-01-10 Innerfield Irving Determination of heparin in the blood

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3512517A (en) * 1964-11-30 1970-05-19 Beckman Instruments Inc Polarographic method and apparatus for monitoring blood glucose concentration
FR2142416A5 (fr) * 1971-06-18 1973-01-26 Boehringer Mannheim Gmbh
US3919051A (en) * 1974-07-11 1975-11-11 Honeywell Inc Biological analyzer and method
US3930957A (en) * 1974-07-11 1976-01-06 Honeywell Inc. Apparatus and method for biological analysis
US3937615A (en) * 1974-12-17 1976-02-10 Leeds & Northrup Company Auto-ranging glucose measuring system

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2372432A1 (fr) * 1976-11-30 1978-06-23 Gambro Ab Procede et dispositif pour mesurer la concentration d'un compose de faible poids moleculaire dans un milieu complexe

Also Published As

Publication number Publication date
CA1086616A (fr) 1980-09-30
DK376677A (da) 1978-03-21
GB1530847A (en) 1978-11-01
BE858791A (fr) 1978-03-16
SE7709357L (sv) 1978-03-21
DD132150A5 (de) 1978-08-30
JPS5339198A (en) 1978-04-10
DE2642232B2 (de) 1979-08-09
DE2642232A1 (de) 1978-03-30
DE2642232C3 (de) 1980-05-14
FR2393306B1 (fr) 1984-05-11
HU180210B (en) 1983-02-28
NL7707199A (nl) 1978-03-22
IT1080795B (it) 1985-05-16
AT352293B (de) 1979-09-10
CH634108A5 (de) 1983-01-14
US4153513A (en) 1979-05-08
ATA430077A (de) 1979-02-15

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