FI83973B - Ett foerfarande foer immobilisering av cellbundna enzymer. - Google Patents

Ett foerfarande foer immobilisering av cellbundna enzymer. Download PDF

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Publication number
FI83973B
FI83973B FI862533A FI862533A FI83973B FI 83973 B FI83973 B FI 83973B FI 862533 A FI862533 A FI 862533A FI 862533 A FI862533 A FI 862533A FI 83973 B FI83973 B FI 83973B
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FI
Finland
Prior art keywords
cell
glutaraldehyde
cells
water content
enzyme
Prior art date
Application number
FI862533A
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English (en)
Finnish (fi)
Other versions
FI862533A0 (fi
FI83973C (sv
FI862533A (fi
Inventor
Mogens Wuempelmann
Original Assignee
Novo Nordisk As
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Publication date
Application filed by Novo Nordisk As filed Critical Novo Nordisk As
Publication of FI862533A0 publication Critical patent/FI862533A0/fi
Publication of FI862533A publication Critical patent/FI862533A/fi
Publication of FI83973B publication Critical patent/FI83973B/fi
Application granted granted Critical
Publication of FI83973C publication Critical patent/FI83973C/sv

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/24—Preparation of compounds containing saccharide radicals produced by the action of an isomerase, e.g. fructose
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C12N11/089—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/22—Tryptophan; Tyrosine; Phenylalanine; 3,4-Dihydroxyphenylalanine
    • C12P13/227—Tryptophan

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Claims (7)

1. Förfarande för immobilisering av en vattendispersion av enzymatiskt aktiva mikro-organismceller och cellsubstan-ser genom att sammanblanda en dylik dispersion med en vat-tenhaltig polyazetidinprepolymer, som därefter härdas, kännetecknat av att ett enzymatiskt aktivt mikro-organism-cellslam behandlas med glutaraldehyd, som omfattar 5-40 % av cellslammets torrämne, varvid en partiell tvärbindning äger rum, varefter vatten avlägsnas frän det partiellt tvärbundna cellslammet tili en vattenhalt inom 70-90 % vikt/vikt för bildande av ett pastaaktigt cellslam; den pastaaktiga cellmassan blandas med den vattenhaltiga poly-azetidinprepolymeren, som utgör 5-30 % av cellslammets torrämne, varefter förutnämnda blandning torkas, varvid erhälls en partikelformig immobiliserad enzymprodukt.
2. Förfarande enligt patentkravet 1, kännetecknat av att granulblandningens vattenhalt sänks under cirka 25 vikt-% under härdningen.
3. Förfarande enligt patentkravet 1, kännetecknat av att de enzymatiskt aktiva mikro-organismcellerna innehäller celler frän en glukosisomerasproducerande stam av Strepto-myces murinus -gruppen.
4. Förfarande enligt patentkravet 1, kännetecknat av att de enzymatiskt aktiva mikro-organismcellerna innehäller celler frän en tryptofansyntetasproducerande mikro-orga-nismstam.
5. Förfarande enligt patentkravet 4, kännetecknat av att cellerna härstammar frän E. coli -stam.
FI862533A 1985-06-14 1986-06-13 Ett förfarande för immobilisering av cellbundna enzymer FI83973C (sv)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DK269285A DK152764C (da) 1985-06-14 1985-06-14 Fremgangsmaade til fremstilling af et immobiliseret enzymprodukt
DK269285 1985-06-14

Publications (4)

Publication Number Publication Date
FI862533A0 FI862533A0 (fi) 1986-06-13
FI862533A FI862533A (fi) 1986-12-15
FI83973B true FI83973B (fi) 1991-06-14
FI83973C FI83973C (sv) 1991-09-25

Family

ID=8114686

Family Applications (1)

Application Number Title Priority Date Filing Date
FI862533A FI83973C (sv) 1985-06-14 1986-06-13 Ett förfarande för immobilisering av cellbundna enzymer

Country Status (8)

Country Link
US (1) US4892825A (sv)
EP (1) EP0206687B1 (sv)
JP (1) JPS6255083A (sv)
KR (1) KR940004542B1 (sv)
DE (1) DE3666837D1 (sv)
DK (1) DK152764C (sv)
ES (1) ES8707763A1 (sv)
FI (1) FI83973C (sv)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK111185A (da) * 1985-03-12 1986-09-13 Novo Industri As Xyloseisomerase, fremgangsmaade til fremstilling deraf, immobiliseret xyloseisomerase og fremgangsmaade til isomerisering af glucose til fructose
DK336987D0 (da) * 1987-07-01 1987-07-01 Novo Industri As Immobiliseringsmetode
US5801022A (en) * 1990-08-03 1998-09-01 Vertex Pharmaceuticals, Incorporated Method of producing a product with crosslinked crystals of thermolysin
US5618710A (en) * 1990-08-03 1997-04-08 Vertex Pharmaceuticals, Inc. Crosslinked enzyme crystals
CA2180826A1 (en) 1994-01-13 1995-07-20 Richard J. Ii Stoner Organic disease control system
FR2755143B1 (fr) 1996-10-25 1998-11-27 Rhone Poulenc Nutrition Animal Procede de preparation de l'acide 2-hydroxy-4-methylthio-butyrique par utilisation d'une nitrilase
AU9742398A (en) * 1997-09-09 1999-03-29 Biochemie Gesellschaft Mbh Esterase free enzymes
EP1167521A1 (en) * 2000-06-30 2002-01-02 Aventis Animal Nutrition S.A. Coated enzyme-containing catalyst
JP4866112B2 (ja) * 2005-07-27 2012-02-01 三菱化学株式会社 生体物質構造体及び生体物質構造体の製造方法、並びに、生体物質担持体、対象物質の精製方法、アフィニティークロマトグラフィー用容器、分離用チップ、対象物質の解析方法、対象物質の解析用分離装置、及び、センサーチップ
JP2007101520A (ja) * 2005-09-09 2007-04-19 Mitsubishi Chemicals Corp 生体物質複合体、並びに、生体物質複合体担持体、対象物質の精製方法、アフィニティークロマトグラフィー用容器、分離用チップ、対象物質の解析方法、対象物質の解析用分離装置及びセンサーチップ
WO2007036235A1 (en) 2005-09-30 2007-04-05 Novozymes A/S Immobilization of enzymes
EP2313501A4 (en) * 2008-03-17 2012-04-04 Immortazyme Company PROCESS FOR PRODUCING BIO-GEL AND CORRESPONDING BIO-GEL
WO2014067933A1 (en) 2012-10-31 2014-05-08 C-Lecta Gmbh Bioactive carrier preparation for enhanced safety in care products and food
CN110982708B (zh) * 2019-12-23 2022-09-06 云南大学 一株丽灰球真菌及其在重金属污染环境生物修复中的应用

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3779869A (en) * 1971-05-13 1973-12-18 Miles Lab Enzyme stabilization
US3980521A (en) * 1974-08-28 1976-09-14 Novo Industri A/S Immobilization of glucose isomerase
US3974036A (en) * 1974-09-03 1976-08-10 Miles Laboratories, Inc. Process for conditioning bacterial cells containing glucose isomerase activity
DK146942C (da) * 1978-04-19 1984-07-30 Novo Industri As Fremgangsmaade til fremstilling af et immobiliseret enzymprodukt
US4355105A (en) * 1981-03-30 1982-10-19 Miles Laboratories, Inc. Glutaraldehyde/polyethylenimine immobilization of whole microbial cells
US4436813A (en) * 1982-03-16 1984-03-13 Purification Engineering, Inc. Immobilized microbial cell composition for making L-aspartic acid
US4732851A (en) * 1982-03-16 1988-03-22 Purification Engineering, Inc. Immobilization of cells with a polyazetidine prepolymer
US4600692A (en) * 1983-02-10 1986-07-15 Purification Engineering, Inc. Immobilized cells for preparing phenylalanine

Also Published As

Publication number Publication date
US4892825A (en) 1990-01-09
DE3666837D1 (en) 1989-12-14
FI862533A0 (fi) 1986-06-13
DK152764B (da) 1988-05-09
JPS6255083A (ja) 1987-03-10
DK269285A (da) 1986-12-15
DK152764C (da) 1988-10-03
ES556019A0 (es) 1987-08-16
EP0206687A1 (en) 1986-12-30
ES8707763A1 (es) 1987-08-16
FI83973C (sv) 1991-09-25
FI862533A (fi) 1986-12-15
KR940004542B1 (ko) 1994-05-25
KR870000425A (ko) 1987-02-18
DK269285D0 (da) 1985-06-14
EP0206687B1 (en) 1989-11-08

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Owner name: NOVOZYMES A/S