FI76216B - Bestaemningsfoerfarande foer antigen. - Google Patents
Bestaemningsfoerfarande foer antigen. Download PDFInfo
- Publication number
- FI76216B FI76216B FI831095A FI831095A FI76216B FI 76216 B FI76216 B FI 76216B FI 831095 A FI831095 A FI 831095A FI 831095 A FI831095 A FI 831095A FI 76216 B FI76216 B FI 76216B
- Authority
- FI
- Finland
- Prior art keywords
- antigen
- buffer
- reaction
- antibody
- polyethylene glycol
- Prior art date
Links
- 239000000427 antigen Substances 0.000 title claims abstract description 14
- 102000036639 antigens Human genes 0.000 title claims abstract description 14
- 108091007433 antigens Proteins 0.000 title claims abstract description 14
- 238000006243 chemical reaction Methods 0.000 claims abstract description 28
- 238000000034 method Methods 0.000 claims abstract description 17
- 229920001223 polyethylene glycol Polymers 0.000 claims abstract description 10
- 239000002202 Polyethylene glycol Substances 0.000 claims abstract description 9
- 239000000872 buffer Substances 0.000 claims description 13
- 239000003795 chemical substances by application Substances 0.000 claims description 3
- 239000004698 Polyethylene Substances 0.000 claims description 2
- -1 polyethylene Polymers 0.000 claims description 2
- 229920000573 polyethylene Polymers 0.000 claims description 2
- ZPUCINDJVBIVPJ-LJISPDSOSA-N cocaine Chemical compound O([C@H]1C[C@@H]2CC[C@@H](N2C)[C@H]1C(=O)OC)C(=O)C1=CC=CC=C1 ZPUCINDJVBIVPJ-LJISPDSOSA-N 0.000 claims 6
- 229960003920 cocaine Drugs 0.000 claims 3
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims 2
- 229940125717 barbiturate Drugs 0.000 claims 2
- HNYOPLTXPVRDBG-UHFFFAOYSA-N barbituric acid Chemical compound O=C1CC(=O)NC(=O)N1 HNYOPLTXPVRDBG-UHFFFAOYSA-N 0.000 claims 2
- 239000002253 acid Substances 0.000 claims 1
- 239000003146 anticoagulant agent Substances 0.000 claims 1
- 229940127219 anticoagulant drug Drugs 0.000 claims 1
- 239000000571 coke Substances 0.000 claims 1
- 230000001404 mediated effect Effects 0.000 claims 1
- 238000005375 photometry Methods 0.000 claims 1
- 239000002504 physiological saline solution Substances 0.000 abstract description 6
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 abstract 2
- 239000012928 buffer substance Substances 0.000 abstract 2
- YMAWOPBAYDPSLA-UHFFFAOYSA-N glycylglycine Chemical compound [NH3+]CC(=O)NCC([O-])=O YMAWOPBAYDPSLA-UHFFFAOYSA-N 0.000 abstract 2
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 abstract 1
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 abstract 1
- 239000004471 Glycine Substances 0.000 abstract 1
- 108010008488 Glycylglycine Proteins 0.000 abstract 1
- HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 abstract 1
- FSVCELGFZIQNCK-UHFFFAOYSA-N N,N-bis(2-hydroxyethyl)glycine Chemical compound OCCN(CCO)CC(O)=O FSVCELGFZIQNCK-UHFFFAOYSA-N 0.000 abstract 1
- 239000007853 buffer solution Substances 0.000 abstract 1
- 238000007865 diluting Methods 0.000 abstract 1
- 229940043257 glycylglycine Drugs 0.000 abstract 1
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 abstract 1
- BDERNNFJNOPAEC-UHFFFAOYSA-N propan-1-ol Chemical compound CCCO BDERNNFJNOPAEC-UHFFFAOYSA-N 0.000 abstract 1
- 238000005259 measurement Methods 0.000 description 5
- 239000003085 diluting agent Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- FTOAOBMCPZCFFF-UHFFFAOYSA-N 5,5-diethylbarbituric acid Chemical compound CCC1(CC)C(=O)NC(=O)NC1=O FTOAOBMCPZCFFF-UHFFFAOYSA-N 0.000 description 2
- 108060003951 Immunoglobulin Proteins 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 102000018358 immunoglobulin Human genes 0.000 description 1
- 229940072221 immunoglobulins Drugs 0.000 description 1
- 230000002045 lasting effect Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000004848 nephelometry Methods 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000012085 test solution Substances 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/557—Immunoassay; Biospecific binding assay; Materials therefor using kinetic measurement, i.e. time rate of progress of an antigen-antibody interaction
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Peptides Or Proteins (AREA)
- Semiconductor Memories (AREA)
- Amplifiers (AREA)
- Junction Field-Effect Transistors (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Claims (7)
1. Förfarande mad vilkat proveta antigenhalt beetamme pä grund av en antigen-antikropperaaktion eä att ätminetona dan ana av deeea reaktionekomponantar utapäde mad an fyeiolo-giek kokealtloaning innahällande polyatylanglykol och buf- fertmedal, därefter bäda reaktionekomponantar föranae och antigan-antikropperaaktion beetamme fotomatriekt, kännatacknat av att buffartmadlet är 5,5-dialkylbarbitureyrabuffart.
2. Förfarande enligt patentkravat 1, kännatacknat av att barbitureyran är an 5,5-diatylbarbitureyra.
3. Förfarande enligt patentkravat 1 allar 2, känneteck-nat av att buffartmedlate koncentration i kokealtlöeningan är 5-200 mmol/1, foraträdaevie 20-50 mmol/1.
4. Förfarande enligt nägot av patantkraven ovan, kännatacknat av att polyetylenglykolene koncentration i kokealtlöeningan är 2-6 vikt-X, företrädaevie cirka 4 vikt-X och polyetylenglykolene ganomenittliga molakylvikt är 4000-12 000, företrädaevie cirka 6000.
5. Förfarande enligt nägot av patantkraven ovan, kännatacknat av att pH-värde av dan buffertmedal innehäl-landa polyatylanglykolhaltiga fyeiologieka kokealtlöeningan är 4,0-9,0 och företrädaevie 6,5-8.
6. Förfarande enligt nägot av patantkraven ovan, kännatacknat av att antigen-antikroppereaktionene kinatieka förlopp beetämmä fotomatriekt.
7. Fyeiologiek kokealtloaning, eom innahällar polyety-langlykol och buffertmedal, eom utepädningeämna ätminetone i dan andra av tvä antigan-antikropperaaktione raaktionekompo-nantar, vilkae antigenhalt baetämme fotomatriekt, känneteck-nad av att buffartmadlet är 5,5-dialkylbarbitureyrabuffart.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19823212658 DE3212658C3 (de) | 1982-04-05 | 1982-04-05 | Verfahren zur antigen-bestimmung |
| DE3212658 | 1982-04-05 |
Publications (4)
| Publication Number | Publication Date |
|---|---|
| FI831095A0 FI831095A0 (fi) | 1983-03-30 |
| FI831095L FI831095L (fi) | 1983-10-06 |
| FI76216B true FI76216B (fi) | 1988-05-31 |
| FI76216C FI76216C (sv) | 1988-09-09 |
Family
ID=6160289
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| FI831095A FI76216C (sv) | 1982-04-05 | 1983-03-30 | Bestämningsförfarande för antigen |
Country Status (8)
| Country | Link |
|---|---|
| EP (1) | EP0090966B1 (sv) |
| JP (1) | JPH0650313B2 (sv) |
| AT (1) | ATE17049T1 (sv) |
| CA (1) | CA1208548A (sv) |
| DE (1) | DE3212658C3 (sv) |
| ES (1) | ES521137A0 (sv) |
| FI (1) | FI76216C (sv) |
| IE (1) | IE54400B1 (sv) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS61113271A (ja) * | 1984-11-08 | 1986-05-31 | Matsushita Electronics Corp | 半導体記憶素子 |
| JPS62159047A (ja) * | 1985-12-31 | 1987-07-15 | Chemo Sero Therapeut Res Inst | 血漿蛋白定量用試薬 |
| US5371021A (en) * | 1992-07-17 | 1994-12-06 | Beckman Instruments, Inc. | Initial rate photometric method for immunoassay |
| TWI842764B (zh) * | 2018-11-09 | 2024-05-21 | 日商積水醫療股份有限公司 | 抑制以自動分析裝置進行的免疫測定中之異常檢測的方法、檢測方法、免疫測定試劑、及免疫測定試劑套組 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1540098A (en) * | 1975-03-06 | 1979-02-07 | Baxter Travenol Lab | Immunological reagent and nephelometric method of using same |
| JPS605899B2 (ja) * | 1975-04-10 | 1985-02-14 | 中外製薬株式会社 | 免疫化学的定量法 |
| GB1590525A (en) * | 1976-12-10 | 1981-06-03 | Technicon Instr | Biological analysis |
| JPS5399319A (en) * | 1977-02-09 | 1978-08-30 | Hidematsu Hirai | Novel qualitative and quantitative detecting method and detecting body for antgenic substance |
| JPS582660A (ja) * | 1981-06-30 | 1983-01-08 | Chugai Pharmaceut Co Ltd | 免疫学的試薬 |
| JPS5847256A (ja) * | 1981-09-14 | 1983-03-18 | Mitsubishi Chem Ind Ltd | 抗原抗体反応の測定法 |
-
1982
- 1982-04-05 DE DE19823212658 patent/DE3212658C3/de not_active Expired - Lifetime
-
1983
- 1983-03-15 EP EP83102566A patent/EP0090966B1/de not_active Expired
- 1983-03-15 AT AT83102566T patent/ATE17049T1/de not_active IP Right Cessation
- 1983-03-25 JP JP58049111A patent/JPH0650313B2/ja not_active Expired - Lifetime
- 1983-03-30 FI FI831095A patent/FI76216C/sv not_active IP Right Cessation
- 1983-03-30 ES ES521137A patent/ES521137A0/es active Granted
- 1983-03-31 CA CA000425012A patent/CA1208548A/en not_active Expired
- 1983-03-31 IE IE752/83A patent/IE54400B1/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| DE3212658C2 (sv) | 1987-11-26 |
| IE54400B1 (en) | 1989-09-27 |
| ES8402425A1 (es) | 1984-01-16 |
| DE3212658A1 (de) | 1983-10-06 |
| DE3212658C3 (de) | 1993-08-19 |
| FI831095L (fi) | 1983-10-06 |
| IE830752L (en) | 1983-10-05 |
| JPS58182558A (ja) | 1983-10-25 |
| ES521137A0 (es) | 1984-01-16 |
| FI831095A0 (fi) | 1983-03-30 |
| JPH0650313B2 (ja) | 1994-06-29 |
| EP0090966B1 (de) | 1985-12-18 |
| ATE17049T1 (de) | 1986-01-15 |
| EP0090966A1 (de) | 1983-10-12 |
| FI76216C (sv) | 1988-09-09 |
| CA1208548A (en) | 1986-07-29 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MM | Patent lapsed |
Owner name: BAXTER INTERNATIONAL INC. |