FI115636B - Kininsyrasyntes från glukos - Google Patents

Kininsyrasyntes från glukos Download PDF

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Publication number
FI115636B
FI115636B FI951499A FI951499A FI115636B FI 115636 B FI115636 B FI 115636B FI 951499 A FI951499 A FI 951499A FI 951499 A FI951499 A FI 951499A FI 115636 B FI115636 B FI 115636B
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FI
Finland
Prior art keywords
dehydroquinate
gene
host cell
quinic acid
pathway
Prior art date
Application number
FI951499A
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English (en)
Finnish (fi)
Other versions
FI951499A (sv
FI951499A0 (sv
Inventor
John W Frost
Karen Draths
Timothy L Ward
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Purdue Research Foundation
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Publication of FI951499A publication Critical patent/FI951499A/sv
Publication of FI951499A0 publication Critical patent/FI951499A0/sv
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Publication of FI115636B publication Critical patent/FI115636B/sv

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/24—Preparation of oxygen-containing organic compounds containing a carbonyl group
    • C12P7/26—Ketones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/42—Hydroxy-carboxylic acids
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/59—Biological synthesis; Biological purification

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Nitrogen Condensed Heterocyclic Rings (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Enzymes And Modification Thereof (AREA)

Claims (11)

1. Förfarande för framställning av en kininsyra eller derivat därav omfattande bensokinon och hydrokinon, 5 kännetecknat av att: a) en värdcell som förmär syntetisera dehydrokinat vaij s, b) omvandling av dehydrokinat tili dehydroshikimat förhindras pä en bana av värdcellen, 10 c) eventuellt introduceras i värdcellen en förmäga att omvandla dehydrokinat tili kininsyra om en dylik förmäga inte är endogen hos värdcellen, d) strömningen av koi frän en förnybar kolkälla tili värdcellens bana ökas, 15 under förutsättning att stegen b) , c) och d) kan utföras i vilken ordning som heist eller samtidigt och e) cellerna odlas i närvaro av en förnybar kolkälla.
2. Förfarande enligt patentkrav 1, känne tecknat av att banan blockeras efter att dehydro- 20 kinat har bildats tili följd av att dehydrokinatdehydratas saknas i värdcellen.
; : 3. Förfarande enligt patentkrav 2, k ä n n e - • : tecknat av att genen, som kodar för dehydrokinatde- :· hydratas i värdcellen, har avlägsnats helt eller delvis 25 eller den har genomgätt mutation.
4. Förfarande enligt patent krav 3, k ä n n e - • · tecknat av att den avlägsnade genen är aroD. * * *
5. Förfarande enligt patentkrav 4, k ä n n e - tecknat av att värdcellstammen är Escherichia coli- 30 stammen AB2848aroD/pkD136, ATCC depositionsnummer 69086.
* < '···' 6. Förfarande enligt patentkrav 5, k ä n n e - ··· tecknat av att förmägan att omvandla dehydrokinat tili kininsyra är en följd av introduktionen i värdcellen av en för värdcellen endogen gen som kodar för kinatdehyd- ;; · 35 rogenas av Klebsiella pneumoniae, där värdcellen är av släktet Escheria. 115636
7. Förfarande enligt patentkrav 6, kanne- tecknat av att en gad-gen introduceras.
8. Förfarande enligt patentkrav 7, k ä n n e - tecknat av att gad-genen introduceras i plasmid 5 pTW6135 (figur 3B) eller pTW8090A (figur 3C) som förmar uttrycka gad-genen, varvid nämnda plasmider är tillgängli-ga genom att utföra förfarandet i figurerna 3A-3C.
9. Förfarande enligt patentkrav 1, kanne- tecknat av att strömningen av koi tili banan ökas 10 genom att transformera värdcellerna med rekombinant DNA som omfattar en gen som kodar för transketolas, en gen som kodar för en isozym av DAPH-syntas och en gen som kodar för 3-dehydrokinatsyntas.
10. Förfarande enligt patentkrav 9, kanne- 15 tecknat av att generna är tkt, aroF och aroB.
11. Kromosomalt element eller ett yttre genetiskt element av en kromosom vilket innehäller en eller flera kopior av gad-gener som isolerats frän Klebsiella pneumoniae. * * » » ♦ * ♦ * · • « ‘ · » # » » ’ I ♦
FI951499A 1992-09-30 1995-03-29 Kininsyrasyntes från glukos FI115636B (sv)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US07/954,623 US5798236A (en) 1992-09-30 1992-09-30 Synthesis of quinic acid from glucose
US95462392 1992-09-30
US9309005 1993-09-23
PCT/US1993/009005 WO1994008015A1 (en) 1992-09-30 1993-09-23 Synthesis of quinic acid from glucose

Publications (3)

Publication Number Publication Date
FI951499A FI951499A (sv) 1995-03-29
FI951499A0 FI951499A0 (sv) 1995-03-29
FI115636B true FI115636B (sv) 2005-06-15

Family

ID=25495703

Family Applications (1)

Application Number Title Priority Date Filing Date
FI951499A FI115636B (sv) 1992-09-30 1995-03-29 Kininsyrasyntes från glukos

Country Status (9)

Country Link
US (1) US5798236A (sv)
EP (1) EP0663011B1 (sv)
JP (2) JP3464481B2 (sv)
AT (1) ATE259880T1 (sv)
CA (1) CA2146025C (sv)
DE (1) DE69333419T2 (sv)
DK (1) DK0663011T3 (sv)
FI (1) FI115636B (sv)
WO (1) WO1994008015A1 (sv)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69637492T2 (de) 1995-05-05 2009-06-04 Genencor International, Inc., Palo Alto Anwendung von Glukosetransportmutanten zur Herstellung von Verbindungen des aromatischen Syntheseweges
US6210937B1 (en) 1997-04-22 2001-04-03 Bechtel Bwxt Idaho, Llc Development of genetically engineered bacteria for production of selected aromatic compounds
US6613552B1 (en) * 1999-01-29 2003-09-02 Board Of Trustees Operating Michigan State University Biocatalytic synthesis of shikimic acid
US6600077B1 (en) * 1999-01-29 2003-07-29 Board Of Trustees Operating Michigan State University Biocatalytic synthesis of quinic acid and conversion to hydroquinone
US6472190B1 (en) * 2000-03-16 2002-10-29 Board Of Trustees Operating Michigan State Univerisity Biocatalytic synthesis of galloid organics
WO2005030949A1 (en) * 2003-09-24 2005-04-07 Board Of Trustees Operating Michigan State University Methods and materials for the production of shikimic acid
EP1899453B1 (en) 2005-06-07 2013-12-18 Ocean Nutrition Canada Limited Eukaryotic microorganisms for producing lipids and antioxidants
US7977077B2 (en) 2006-01-30 2011-07-12 Board Of Trustees Of Michigan State University Synthesis of intermediates of oseltamivir carboxylates
US9023616B2 (en) * 2006-08-01 2015-05-05 Dsm Nutritional Products Ag Oil producing microbes and method of modification thereof
PT2970926T (pt) 2013-03-13 2018-03-22 Dsm Nutritional Products Ag Modificação genética de microrganismos
KR101911186B1 (ko) * 2017-08-16 2018-10-25 씨제이제일제당 (주) 마이코스포린 유사 아미노산을 생산하는 미생물 및 이를 이용한 마이코스포린 유사 아미노산의 생산방법
KR102003911B1 (ko) * 2018-02-23 2019-07-25 씨제이제일제당 주식회사 마이코스포린 유사 아미노산을 생산하는 미생물 및 이를 이용한 마이코스포린 유사 아미노산의 생산방법
CN108641994B (zh) * 2018-05-21 2021-12-21 中国科学院天津工业生物技术研究所 生产奎尼酸相关的大肠杆菌重组菌株及其构建方法与应用
WO2024038063A1 (en) * 2022-08-16 2024-02-22 South China Botanical Garden Pesticidal compositions

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5168056A (en) * 1991-02-08 1992-12-01 Purdue Research Foundation Enhanced production of common aromatic pathway compounds

Also Published As

Publication number Publication date
FI951499A (sv) 1995-03-29
DK0663011T3 (da) 2004-06-21
WO1994008015A1 (en) 1994-04-14
CA2146025A1 (en) 1994-04-14
US5798236A (en) 1998-08-25
JP3464481B2 (ja) 2003-11-10
FI951499A0 (sv) 1995-03-29
EP0663011B1 (en) 2004-02-18
ATE259880T1 (de) 2004-03-15
DE69333419T2 (de) 2004-12-16
CA2146025C (en) 2007-07-10
JP3550144B2 (ja) 2004-08-04
JPH08502165A (ja) 1996-03-12
EP0663011A1 (en) 1995-07-19
DE69333419D1 (de) 2004-03-25
JP2003299493A (ja) 2003-10-21

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