FI111013B - Stabila purA-vektorer och deras användning - Google Patents
Stabila purA-vektorer och deras användning Download PDFInfo
- Publication number
- FI111013B FI111013B FI921940A FI921940A FI111013B FI 111013 B FI111013 B FI 111013B FI 921940 A FI921940 A FI 921940A FI 921940 A FI921940 A FI 921940A FI 111013 B FI111013 B FI 111013B
- Authority
- FI
- Finland
- Prior art keywords
- pura
- plasmid
- gene
- virus
- vector
- Prior art date
Links
- 101150002764 purA gene Proteins 0.000 title claims abstract description 101
- 239000013598 vector Substances 0.000 title claims abstract description 55
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 115
- 241000894006 Bacteria Species 0.000 claims abstract description 47
- 229960005486 vaccine Drugs 0.000 claims abstract description 42
- 230000002238 attenuated effect Effects 0.000 claims abstract description 31
- 230000001580 bacterial effect Effects 0.000 claims abstract description 30
- 239000013612 plasmid Substances 0.000 claims description 150
- 239000000427 antigen Substances 0.000 claims description 46
- 108091007433 antigens Proteins 0.000 claims description 46
- 102000036639 antigens Human genes 0.000 claims description 46
- 238000000034 method Methods 0.000 claims description 36
- 241000588724 Escherichia coli Species 0.000 claims description 35
- 102000004169 proteins and genes Human genes 0.000 claims description 35
- 241000607142 Salmonella Species 0.000 claims description 33
- 210000004027 cell Anatomy 0.000 claims description 33
- 241001465754 Metazoa Species 0.000 claims description 29
- 229930024421 Adenine Natural products 0.000 claims description 17
- 229960000643 adenine Drugs 0.000 claims description 17
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 claims description 16
- 241000700605 Viruses Species 0.000 claims description 15
- 230000015572 biosynthetic process Effects 0.000 claims description 15
- 239000012634 fragment Substances 0.000 claims description 11
- 238000003786 synthesis reaction Methods 0.000 claims description 9
- 108090000364 Ligases Proteins 0.000 claims description 7
- 102000003960 Ligases Human genes 0.000 claims description 7
- 230000003612 virological effect Effects 0.000 claims description 7
- 108020001507 fusion proteins Proteins 0.000 claims description 6
- 102000037865 fusion proteins Human genes 0.000 claims description 6
- 201000004792 malaria Diseases 0.000 claims description 6
- 244000052769 pathogen Species 0.000 claims description 6
- 230000001105 regulatory effect Effects 0.000 claims description 6
- -1 adenyl succinate Chemical compound 0.000 claims description 5
- 239000001963 growth medium Substances 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 5
- 239000002773 nucleotide Substances 0.000 claims description 5
- 125000003729 nucleotide group Chemical group 0.000 claims description 5
- 230000001717 pathogenic effect Effects 0.000 claims description 5
- 206010061765 Chromosomal mutation Diseases 0.000 claims description 4
- 150000001491 aromatic compounds Chemical class 0.000 claims description 4
- 230000002829 reductive effect Effects 0.000 claims description 4
- 101710146739 Enterotoxin Proteins 0.000 claims description 3
- 241000606768 Haemophilus influenzae Species 0.000 claims description 3
- 241000725303 Human immunodeficiency virus Species 0.000 claims description 3
- 241000588655 Moraxella catarrhalis Species 0.000 claims description 3
- 241000607626 Vibrio cholerae Species 0.000 claims description 3
- 239000000147 enterotoxin Substances 0.000 claims description 3
- 231100000655 enterotoxin Toxicity 0.000 claims description 3
- 229940047650 haemophilus influenzae Drugs 0.000 claims description 3
- 244000045947 parasite Species 0.000 claims description 3
- 229940118696 vibrio cholerae Drugs 0.000 claims description 3
- 241000711573 Coronaviridae Species 0.000 claims description 2
- 241000701022 Cytomegalovirus Species 0.000 claims description 2
- 241000991587 Enterovirus C Species 0.000 claims description 2
- 208000005176 Hepatitis C Diseases 0.000 claims description 2
- 241000588747 Klebsiella pneumoniae Species 0.000 claims description 2
- 241000712079 Measles morbillivirus Species 0.000 claims description 2
- 241001631646 Papillomaviridae Species 0.000 claims description 2
- 241000702670 Rotavirus Species 0.000 claims description 2
- 241000700584 Simplexvirus Species 0.000 claims description 2
- 241000191967 Staphylococcus aureus Species 0.000 claims description 2
- 241000194017 Streptococcus Species 0.000 claims description 2
- 241000193996 Streptococcus pyogenes Species 0.000 claims description 2
- 239000003795 chemical substances by application Substances 0.000 claims description 2
- 208000006454 hepatitis Diseases 0.000 claims description 2
- 231100000283 hepatitis Toxicity 0.000 claims description 2
- 208000005252 hepatitis A Diseases 0.000 claims description 2
- 208000002672 hepatitis B Diseases 0.000 claims description 2
- 230000000968 intestinal effect Effects 0.000 claims description 2
- 230000001937 non-anti-biotic effect Effects 0.000 claims description 2
- 230000008569 process Effects 0.000 claims description 2
- 241000701161 unidentified adenovirus Species 0.000 claims description 2
- 241000712461 unidentified influenza virus Species 0.000 claims description 2
- 241000588722 Escherichia Species 0.000 claims 2
- 241000588832 Bordetella pertussis Species 0.000 claims 1
- 241000589876 Campylobacter Species 0.000 claims 1
- 201000006082 Chickenpox Diseases 0.000 claims 1
- 241000606153 Chlamydia trachomatis Species 0.000 claims 1
- 101710117490 Circumsporozoite protein Proteins 0.000 claims 1
- 241000272470 Circus Species 0.000 claims 1
- 241000193403 Clostridium Species 0.000 claims 1
- 241000186216 Corynebacterium Species 0.000 claims 1
- 241000233866 Fungi Species 0.000 claims 1
- 206010064571 Gene mutation Diseases 0.000 claims 1
- 241000701044 Human gammaherpesvirus 4 Species 0.000 claims 1
- 241000588652 Neisseria gonorrhoeae Species 0.000 claims 1
- 241000588650 Neisseria meningitidis Species 0.000 claims 1
- 208000002606 Paramyxoviridae Infections Diseases 0.000 claims 1
- 241000589516 Pseudomonas Species 0.000 claims 1
- 241000710799 Rubella virus Species 0.000 claims 1
- 241000607768 Shigella Species 0.000 claims 1
- 210000001744 T-lymphocyte Anatomy 0.000 claims 1
- 206010046980 Varicella Diseases 0.000 claims 1
- 241000607598 Vibrio Species 0.000 claims 1
- 208000003152 Yellow Fever Diseases 0.000 claims 1
- 241000607734 Yersinia <bacteria> Species 0.000 claims 1
- 239000002671 adjuvant Substances 0.000 claims 1
- 229940038705 chlamydia trachomatis Drugs 0.000 claims 1
- 230000000241 respiratory effect Effects 0.000 claims 1
- 239000000758 substrate Substances 0.000 claims 1
- 241000293871 Salmonella enterica subsp. enterica serovar Typhi Species 0.000 abstract description 16
- 230000035772 mutation Effects 0.000 abstract description 15
- 230000002759 chromosomal effect Effects 0.000 abstract description 13
- 238000004519 manufacturing process Methods 0.000 abstract description 13
- 238000000855 fermentation Methods 0.000 abstract description 8
- 230000004151 fermentation Effects 0.000 abstract description 8
- 206010061764 Chromosomal deletion Diseases 0.000 abstract description 6
- 108010056443 Adenylosuccinate synthase Proteins 0.000 abstract description 5
- 230000003115 biocidal effect Effects 0.000 abstract description 3
- 238000012423 maintenance Methods 0.000 abstract description 3
- 102000005291 Adenylosuccinate synthase Human genes 0.000 abstract description 2
- 230000027086 plasmid maintenance Effects 0.000 abstract description 2
- 230000014509 gene expression Effects 0.000 description 59
- 235000018102 proteins Nutrition 0.000 description 30
- 241000293869 Salmonella enterica subsp. enterica serovar Typhimurium Species 0.000 description 28
- 239000000047 product Substances 0.000 description 17
- 230000012010 growth Effects 0.000 description 16
- 230000009469 supplementation Effects 0.000 description 16
- 238000010367 cloning Methods 0.000 description 13
- 239000002609 medium Substances 0.000 description 13
- 241000894007 species Species 0.000 description 13
- 108020004414 DNA Proteins 0.000 description 12
- 210000000349 chromosome Anatomy 0.000 description 11
- 230000006641 stabilisation Effects 0.000 description 11
- 238000011105 stabilization Methods 0.000 description 11
- 230000014616 translation Effects 0.000 description 11
- 238000000338 in vitro Methods 0.000 description 10
- 238000001727 in vivo Methods 0.000 description 10
- 229930027917 kanamycin Natural products 0.000 description 10
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 10
- 229960000318 kanamycin Drugs 0.000 description 10
- 229930182823 kanamycin A Natural products 0.000 description 10
- 238000013519 translation Methods 0.000 description 10
- 108020004511 Recombinant DNA Proteins 0.000 description 9
- 229940124842 Salmonella vaccine Drugs 0.000 description 9
- 238000012217 deletion Methods 0.000 description 9
- 230000037430 deletion Effects 0.000 description 9
- 239000013613 expression plasmid Substances 0.000 description 9
- 230000006870 function Effects 0.000 description 9
- 239000013600 plasmid vector Substances 0.000 description 9
- KDCGOANMDULRCW-UHFFFAOYSA-N 7H-purine Chemical compound N1=CNC2=NC=NC2=C1 KDCGOANMDULRCW-UHFFFAOYSA-N 0.000 description 8
- 241000699670 Mus sp. Species 0.000 description 8
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 8
- 230000028993 immune response Effects 0.000 description 8
- 108020004999 messenger RNA Proteins 0.000 description 8
- 230000010076 replication Effects 0.000 description 8
- 210000000952 spleen Anatomy 0.000 description 8
- 238000013518 transcription Methods 0.000 description 8
- 230000035897 transcription Effects 0.000 description 8
- 238000002255 vaccination Methods 0.000 description 8
- 241000392514 Salmonella enterica subsp. enterica serovar Dublin Species 0.000 description 7
- 230000000295 complement effect Effects 0.000 description 7
- 201000010099 disease Diseases 0.000 description 7
- 230000002068 genetic effect Effects 0.000 description 7
- 230000003053 immunization Effects 0.000 description 7
- 210000004185 liver Anatomy 0.000 description 7
- 230000026683 transduction Effects 0.000 description 7
- 238000010361 transduction Methods 0.000 description 7
- 241001167018 Aroa Species 0.000 description 6
- 108091026890 Coding region Proteins 0.000 description 6
- 101100491986 Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) aromA gene Proteins 0.000 description 6
- 102000004190 Enzymes Human genes 0.000 description 6
- 108090000790 Enzymes Proteins 0.000 description 6
- 108091028043 Nucleic acid sequence Proteins 0.000 description 6
- 108091081024 Start codon Proteins 0.000 description 6
- OIRDTQYFTABQOQ-KQYNXXCUSA-N adenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 description 6
- 101150037081 aroA gene Proteins 0.000 description 6
- 230000027455 binding Effects 0.000 description 6
- 230000001419 dependent effect Effects 0.000 description 6
- 229940088598 enzyme Drugs 0.000 description 6
- 208000015181 infectious disease Diseases 0.000 description 6
- 108090000765 processed proteins & peptides Proteins 0.000 description 6
- 238000000638 solvent extraction Methods 0.000 description 6
- 230000009466 transformation Effects 0.000 description 6
- 229920001817 Agar Polymers 0.000 description 5
- 206010059866 Drug resistance Diseases 0.000 description 5
- 239000008272 agar Substances 0.000 description 5
- 239000000969 carrier Substances 0.000 description 5
- 238000010276 construction Methods 0.000 description 5
- 230000007812 deficiency Effects 0.000 description 5
- 230000002950 deficient Effects 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 239000013604 expression vector Substances 0.000 description 5
- 238000002649 immunization Methods 0.000 description 5
- 230000002163 immunogen Effects 0.000 description 5
- 238000002513 implantation Methods 0.000 description 5
- 239000000463 material Substances 0.000 description 5
- 210000001986 peyer's patch Anatomy 0.000 description 5
- 102000004196 processed proteins & peptides Human genes 0.000 description 5
- 230000001681 protective effect Effects 0.000 description 5
- 108091008146 restriction endonucleases Proteins 0.000 description 5
- 102000053602 DNA Human genes 0.000 description 4
- 241000725643 Respiratory syncytial virus Species 0.000 description 4
- 230000000890 antigenic effect Effects 0.000 description 4
- 210000001151 cytotoxic T lymphocyte Anatomy 0.000 description 4
- 238000013461 design Methods 0.000 description 4
- 238000004520 electroporation Methods 0.000 description 4
- 230000010354 integration Effects 0.000 description 4
- 239000003550 marker Substances 0.000 description 4
- 210000000056 organ Anatomy 0.000 description 4
- 230000002441 reversible effect Effects 0.000 description 4
- 238000003860 storage Methods 0.000 description 4
- 210000001519 tissue Anatomy 0.000 description 4
- 239000003053 toxin Substances 0.000 description 4
- 231100000765 toxin Toxicity 0.000 description 4
- 108700012359 toxins Proteins 0.000 description 4
- 230000017105 transposition Effects 0.000 description 4
- 239000002126 C01EB10 - Adenosine Substances 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 241000282412 Homo Species 0.000 description 3
- 239000006137 Luria-Bertani broth Substances 0.000 description 3
- 206010028980 Neoplasm Diseases 0.000 description 3
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 3
- 239000002253 acid Substances 0.000 description 3
- 150000007513 acids Chemical class 0.000 description 3
- 229960005305 adenosine Drugs 0.000 description 3
- 102000005130 adenylosuccinate synthetase Human genes 0.000 description 3
- 235000001014 amino acid Nutrition 0.000 description 3
- 229940024606 amino acid Drugs 0.000 description 3
- 150000001413 amino acids Chemical class 0.000 description 3
- 229960000723 ampicillin Drugs 0.000 description 3
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 3
- 229960001212 bacterial vaccine Drugs 0.000 description 3
- 238000003776 cleavage reaction Methods 0.000 description 3
- 238000012258 culturing Methods 0.000 description 3
- 230000007547 defect Effects 0.000 description 3
- 238000009472 formulation Methods 0.000 description 3
- 230000002538 fungal effect Effects 0.000 description 3
- 238000007429 general method Methods 0.000 description 3
- 230000005847 immunogenicity Effects 0.000 description 3
- 238000010348 incorporation Methods 0.000 description 3
- 230000006698 induction Effects 0.000 description 3
- 238000007912 intraperitoneal administration Methods 0.000 description 3
- 238000001990 intravenous administration Methods 0.000 description 3
- 230000014759 maintenance of location Effects 0.000 description 3
- 230000003071 parasitic effect Effects 0.000 description 3
- 239000002953 phosphate buffered saline Substances 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 230000003362 replicative effect Effects 0.000 description 3
- 230000007017 scission Effects 0.000 description 3
- 238000005204 segregation Methods 0.000 description 3
- 210000005070 sphincter Anatomy 0.000 description 3
- 230000000638 stimulation Effects 0.000 description 3
- 210000002784 stomach Anatomy 0.000 description 3
- 241001515965 unidentified phage Species 0.000 description 3
- WQZGKKKJIJFFOK-SVZMEOIVSA-N (+)-Galactose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-SVZMEOIVSA-N 0.000 description 2
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 2
- GLDQAMYCGOIJDV-UHFFFAOYSA-N 2,3-dihydroxybenzoic acid Chemical compound OC(=O)C1=CC=CC(O)=C1O GLDQAMYCGOIJDV-UHFFFAOYSA-N 0.000 description 2
- ALYNCZNDIQEVRV-UHFFFAOYSA-N 4-aminobenzoic acid Chemical compound NC1=CC=C(C(O)=O)C=C1 ALYNCZNDIQEVRV-UHFFFAOYSA-N 0.000 description 2
- 241000283690 Bos taurus Species 0.000 description 2
- 241000283707 Capra Species 0.000 description 2
- 101100278012 Escherichia coli (strain K12) dnaG gene Proteins 0.000 description 2
- 241001646716 Escherichia coli K-12 Species 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Natural products CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 2
- 241000588653 Neisseria Species 0.000 description 2
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 description 2
- 241000224017 Plasmodium berghei Species 0.000 description 2
- 229920001213 Polysorbate 20 Polymers 0.000 description 2
- 241001354013 Salmonella enterica subsp. enterica serovar Enteritidis Species 0.000 description 2
- 241000607132 Salmonella enterica subsp. enterica serovar Gallinarum Species 0.000 description 2
- 101100165173 Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) basS gene Proteins 0.000 description 2
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 2
- 108010008038 Synthetic Vaccines Proteins 0.000 description 2
- 230000024932 T cell mediated immunity Effects 0.000 description 2
- 101100421924 Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) spo0C gene Proteins 0.000 description 2
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 2
- 208000037386 Typhoid Diseases 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 239000003242 anti bacterial agent Substances 0.000 description 2
- 229940088710 antibiotic agent Drugs 0.000 description 2
- 244000052616 bacterial pathogen Species 0.000 description 2
- 230000006399 behavior Effects 0.000 description 2
- 230000001413 cellular effect Effects 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- 230000002939 deleterious effect Effects 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 238000009826 distribution Methods 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 239000002158 endotoxin Substances 0.000 description 2
- 210000003527 eukaryotic cell Anatomy 0.000 description 2
- 101150004979 flmA gene Proteins 0.000 description 2
- OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 239000012737 fresh medium Substances 0.000 description 2
- 150000004676 glycans Chemical class 0.000 description 2
- 239000003102 growth factor Substances 0.000 description 2
- 238000002744 homologous recombination Methods 0.000 description 2
- 230000006801 homologous recombination Effects 0.000 description 2
- 239000005556 hormone Substances 0.000 description 2
- 229940088597 hormone Drugs 0.000 description 2
- 239000007943 implant Substances 0.000 description 2
- 230000001939 inductive effect Effects 0.000 description 2
- 230000008595 infiltration Effects 0.000 description 2
- 238000001764 infiltration Methods 0.000 description 2
- 229920006008 lipopolysaccharide Polymers 0.000 description 2
- 229940124590 live attenuated vaccine Drugs 0.000 description 2
- 229940023012 live-attenuated vaccine Drugs 0.000 description 2
- 210000003563 lymphoid tissue Anatomy 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000002018 overexpression Effects 0.000 description 2
- 101150048892 parB gene Proteins 0.000 description 2
- 230000002688 persistence Effects 0.000 description 2
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 2
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 2
- 229920001184 polypeptide Polymers 0.000 description 2
- 229920001282 polysaccharide Polymers 0.000 description 2
- 239000005017 polysaccharide Substances 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 229940124551 recombinant vaccine Drugs 0.000 description 2
- 230000006798 recombination Effects 0.000 description 2
- 210000003705 ribosome Anatomy 0.000 description 2
- 238000013112 stability test Methods 0.000 description 2
- 230000010473 stable expression Effects 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 230000001052 transient effect Effects 0.000 description 2
- 201000008297 typhoid fever Diseases 0.000 description 2
- 230000007923 virulence factor Effects 0.000 description 2
- 239000000304 virulence factor Substances 0.000 description 2
- 238000001262 western blot Methods 0.000 description 2
- ITFICYZHWXDVMU-IPTZIORSSA-N (2S)-2-[[(2S)-5-amino-2-[[(2S)-2-[[(2S)-2-[[(2S)-4-amino-2-[[(2S,3S)-2-[[(2S)-2-[[(2S)-5-amino-2-[[(2S)-6-amino-2-[[(2S,3S)-2-[[(2S)-2-[[(2R)-2-[[(2S)-1-[(2S)-2-[[(2S,3R)-2-[[(2S,3S)-2-[[(2S,3R)-2-[[(2S)-2-[[(2S)-2-[[2-[[(2S)-2-amino-4-carboxybutanoyl]amino]acetyl]amino]-3-hydroxypropanoyl]amino]-3-carboxypropanoyl]amino]-3-hydroxybutanoyl]amino]-3-methylpentanoyl]amino]-3-hydroxybutanoyl]amino]-4-methylpentanoyl]pyrrolidine-2-carbonyl]amino]-3-sulfanylpropanoyl]amino]-5-(diaminomethylideneamino)pentanoyl]amino]-3-methylpentanoyl]amino]hexanoyl]amino]-5-oxopentanoyl]amino]-3-phenylpropanoyl]amino]-3-methylpentanoyl]amino]-4-oxobutanoyl]amino]-4-methylsulfanylbutanoyl]amino]-3-(1H-indol-3-yl)propanoyl]amino]-5-oxopentanoyl]amino]pentanedioic acid Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC(=O)N)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CC2=CNC3=CC=CC=C32)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CCC(=O)O)C(=O)O)NC(=O)[C@H](CCCN=C(N)N)NC(=O)[C@H](CS)NC(=O)[C@@H]4CCCN4C(=O)[C@H](CC(C)C)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H]([C@@H](C)CC)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](CC(=O)O)NC(=O)[C@H](CO)NC(=O)CNC(=O)[C@H](CCC(=O)O)N ITFICYZHWXDVMU-IPTZIORSSA-N 0.000 description 1
- 108020004465 16S ribosomal RNA Proteins 0.000 description 1
- NHBKXEKEPDILRR-UHFFFAOYSA-N 2,3-bis(butanoylsulfanyl)propyl butanoate Chemical compound CCCC(=O)OCC(SC(=O)CCC)CSC(=O)CCC NHBKXEKEPDILRR-UHFFFAOYSA-N 0.000 description 1
- 229940082044 2,3-dihydroxybenzoic acid Drugs 0.000 description 1
- 108010068327 4-hydroxyphenylpyruvate dioxygenase Proteins 0.000 description 1
- ZZBAGJPKGRJIJH-UHFFFAOYSA-N 7h-purine-2-carbaldehyde Chemical compound O=CC1=NC=C2NC=NC2=N1 ZZBAGJPKGRJIJH-UHFFFAOYSA-N 0.000 description 1
- 208000030507 AIDS Diseases 0.000 description 1
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 1
- 108700028369 Alleles Proteins 0.000 description 1
- 208000031295 Animal disease Diseases 0.000 description 1
- 101000787278 Arabidopsis thaliana Valine-tRNA ligase, chloroplastic/mitochondrial 2 Proteins 0.000 description 1
- 101000787296 Arabidopsis thaliana Valine-tRNA ligase, mitochondrial 1 Proteins 0.000 description 1
- DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 description 1
- 241000228212 Aspergillus Species 0.000 description 1
- 208000023275 Autoimmune disease Diseases 0.000 description 1
- 241000223836 Babesia Species 0.000 description 1
- 244000063299 Bacillus subtilis Species 0.000 description 1
- 235000014469 Bacillus subtilis Nutrition 0.000 description 1
- 108010077805 Bacterial Proteins Proteins 0.000 description 1
- 208000034309 Bacterial disease carrier Diseases 0.000 description 1
- 231100000699 Bacterial toxin Toxicity 0.000 description 1
- 241000335423 Blastomyces Species 0.000 description 1
- 101150052200 CS gene Proteins 0.000 description 1
- 241000589875 Campylobacter jejuni Species 0.000 description 1
- 241000222120 Candida <Saccharomycetales> Species 0.000 description 1
- 244000089742 Citrus aurantifolia Species 0.000 description 1
- 235000008733 Citrus aurantifolia Nutrition 0.000 description 1
- 241000193449 Clostridium tetani Species 0.000 description 1
- 241000223203 Coccidioides Species 0.000 description 1
- MFYSYFVPBJMHGN-UHFFFAOYSA-N Cortisone Natural products O=C1CCC2(C)C3C(=O)CC(C)(C(CC4)(O)C(=O)CO)C4C3CCC2=C1 MFYSYFVPBJMHGN-UHFFFAOYSA-N 0.000 description 1
- 241001337994 Cryptococcus <scale insect> Species 0.000 description 1
- 102000004127 Cytokines Human genes 0.000 description 1
- 108090000695 Cytokines Proteins 0.000 description 1
- 102000012410 DNA Ligases Human genes 0.000 description 1
- 108010061982 DNA Ligases Proteins 0.000 description 1
- 230000004543 DNA replication Effects 0.000 description 1
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 description 1
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 description 1
- 101710088194 Dehydrogenase Proteins 0.000 description 1
- 206010012735 Diarrhoea Diseases 0.000 description 1
- 101000787280 Dictyostelium discoideum Probable valine-tRNA ligase, mitochondrial Proteins 0.000 description 1
- 108090000204 Dipeptidase 1 Proteins 0.000 description 1
- 241000223924 Eimeria Species 0.000 description 1
- 241000701832 Enterobacteria phage T3 Species 0.000 description 1
- 241000588921 Enterobacteriaceae Species 0.000 description 1
- 241000701959 Escherichia virus Lambda Species 0.000 description 1
- 108700039887 Essential Genes Proteins 0.000 description 1
- 241000206602 Eukaryota Species 0.000 description 1
- 108010040721 Flagellin Proteins 0.000 description 1
- 241001137350 Fratercula Species 0.000 description 1
- 108091006027 G proteins Proteins 0.000 description 1
- 102000030782 GTP binding Human genes 0.000 description 1
- 108091000058 GTP-Binding Proteins 0.000 description 1
- 108700039691 Genetic Promoter Regions Proteins 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 108090000288 Glycoproteins Proteins 0.000 description 1
- 102000003886 Glycoproteins Human genes 0.000 description 1
- 241000700721 Hepatitis B virus Species 0.000 description 1
- 108010001336 Horseradish Peroxidase Proteins 0.000 description 1
- XQFRJNBWHJMXHO-RRKCRQDMSA-N IDUR Chemical compound C1[C@H](O)[C@@H](CO)O[C@H]1N1C(=O)NC(=O)C(I)=C1 XQFRJNBWHJMXHO-RRKCRQDMSA-N 0.000 description 1
- DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 1
- COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 1
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- 241000222722 Leishmania <genus> Species 0.000 description 1
- 108090001030 Lipoproteins Proteins 0.000 description 1
- 102000008072 Lymphokines Human genes 0.000 description 1
- 108010074338 Lymphokines Proteins 0.000 description 1
- 208000002720 Malnutrition Diseases 0.000 description 1
- 101710085938 Matrix protein Proteins 0.000 description 1
- 101710127721 Membrane protein Proteins 0.000 description 1
- OVBPIULPVIDEAO-UHFFFAOYSA-N N-Pteroyl-L-glutaminsaeure Natural products C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)NC(CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-UHFFFAOYSA-N 0.000 description 1
- PYUSHNKNPOHWEZ-YFKPBYRVSA-N N-formyl-L-methionine Chemical group CSCC[C@@H](C(O)=O)NC=O PYUSHNKNPOHWEZ-YFKPBYRVSA-N 0.000 description 1
- 239000000020 Nitrocellulose Substances 0.000 description 1
- 101710141454 Nucleoprotein Proteins 0.000 description 1
- 108091034117 Oligonucleotide Proteins 0.000 description 1
- 101710116435 Outer membrane protein Proteins 0.000 description 1
- 241001537205 Paracoccidioides Species 0.000 description 1
- 241000224016 Plasmodium Species 0.000 description 1
- 241000223960 Plasmodium falciparum Species 0.000 description 1
- 241000233870 Pneumocystis Species 0.000 description 1
- 102000001253 Protein Kinase Human genes 0.000 description 1
- 230000004570 RNA-binding Effects 0.000 description 1
- 102000004879 Racemases and epimerases Human genes 0.000 description 1
- 108090001066 Racemases and epimerases Proteins 0.000 description 1
- 101100373202 Rattus norvegicus Cx3cl1 gene Proteins 0.000 description 1
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 1
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 1
- 108091081062 Repeated sequence (DNA) Proteins 0.000 description 1
- 108700039701 Rotavirus VP4 Proteins 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 241001138501 Salmonella enterica Species 0.000 description 1
- 241000531795 Salmonella enterica subsp. enterica serovar Paratyphi A Species 0.000 description 1
- 241000577483 Salmonella enterica subsp. enterica serovar Paratyphi B Species 0.000 description 1
- 241000607683 Salmonella enterica subsp. enterica serovar Pullorum Species 0.000 description 1
- 241000405383 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 Species 0.000 description 1
- 241000701835 Salmonella virus P22 Species 0.000 description 1
- 241000242678 Schistosoma Species 0.000 description 1
- 229920002684 Sepharose Polymers 0.000 description 1
- 241000607764 Shigella dysenteriae Species 0.000 description 1
- 241000607762 Shigella flexneri Species 0.000 description 1
- 108010052160 Site-specific recombinase Proteins 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 241000193998 Streptococcus pneumoniae Species 0.000 description 1
- 101710172711 Structural protein Proteins 0.000 description 1
- 241000282887 Suidae Species 0.000 description 1
- 102100021696 Syncytin-1 Human genes 0.000 description 1
- 230000005867 T cell response Effects 0.000 description 1
- 208000000389 T-cell leukemia Diseases 0.000 description 1
- 208000028530 T-cell lymphoblastic leukemia/lymphoma Diseases 0.000 description 1
- 244000269722 Thea sinensis Species 0.000 description 1
- JZRWCGZRTZMZEH-UHFFFAOYSA-N Thiamine Natural products CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N JZRWCGZRTZMZEH-UHFFFAOYSA-N 0.000 description 1
- 208000007536 Thrombosis Diseases 0.000 description 1
- 241001664469 Tibicina haematodes Species 0.000 description 1
- 235000011941 Tilia x europaea Nutrition 0.000 description 1
- 241000223996 Toxoplasma Species 0.000 description 1
- 102000008579 Transposases Human genes 0.000 description 1
- 108010020764 Transposases Proteins 0.000 description 1
- 241000223104 Trypanosoma Species 0.000 description 1
- 102000013625 Valine-tRNA Ligase Human genes 0.000 description 1
- 108010003533 Viral Envelope Proteins Proteins 0.000 description 1
- 241000409279 Xerochrysium dermatitidis Species 0.000 description 1
- 241000710772 Yellow fever virus Species 0.000 description 1
- 241000607447 Yersinia enterocolitica Species 0.000 description 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 239000013566 allergen Substances 0.000 description 1
- 125000000539 amino acid group Chemical group 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 230000000469 anti-sperm effect Effects 0.000 description 1
- 210000000612 antigen-presenting cell Anatomy 0.000 description 1
- 125000003118 aryl group Chemical group 0.000 description 1
- 229960001230 asparagine Drugs 0.000 description 1
- 235000009582 asparagine Nutrition 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 239000000688 bacterial toxin Substances 0.000 description 1
- 239000013602 bacteriophage vector Substances 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 229960000074 biopharmaceutical Drugs 0.000 description 1
- 230000006696 biosynthetic metabolic pathway Effects 0.000 description 1
- 210000004899 c-terminal region Anatomy 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000030833 cell death Effects 0.000 description 1
- 230000003833 cell viability Effects 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000001143 conditioned effect Effects 0.000 description 1
- 230000021615 conjugation Effects 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 235000020805 dietary restrictions Nutrition 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 1
- 230000008034 disappearance Effects 0.000 description 1
- 208000035475 disorder Diseases 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000000428 dust Substances 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000009585 enzyme analysis Methods 0.000 description 1
- 210000000981 epithelium Anatomy 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 229960000304 folic acid Drugs 0.000 description 1
- 235000019152 folic acid Nutrition 0.000 description 1
- 239000011724 folic acid Substances 0.000 description 1
- 229930182830 galactose Natural products 0.000 description 1
- 230000005251 gamma ray Effects 0.000 description 1
- 230000002496 gastric effect Effects 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 238000012224 gene deletion Methods 0.000 description 1
- 230000008303 genetic mechanism Effects 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 239000000185 hemagglutinin Substances 0.000 description 1
- 230000028996 humoral immune response Effects 0.000 description 1
- 230000004727 humoral immunity Effects 0.000 description 1
- 230000008348 humoral response Effects 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 239000000411 inducer Substances 0.000 description 1
- 206010022000 influenza Diseases 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 238000007918 intramuscular administration Methods 0.000 description 1
- 230000006623 intrinsic pathway Effects 0.000 description 1
- 230000002427 irreversible effect Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 101150066555 lacZ gene Proteins 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 239000004571 lime Substances 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 210000005229 liver cell Anatomy 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 230000001320 lysogenic effect Effects 0.000 description 1
- 229940124735 malaria vaccine Drugs 0.000 description 1
- 230000003211 malignant effect Effects 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- MYWUZJCMWCOHBA-VIFPVBQESA-N methamphetamine Chemical compound CN[C@@H](C)CC1=CC=CC=C1 MYWUZJCMWCOHBA-VIFPVBQESA-N 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 210000000865 mononuclear phagocyte system Anatomy 0.000 description 1
- 150000004712 monophosphates Chemical class 0.000 description 1
- 101150079289 mutA gene Proteins 0.000 description 1
- 238000002703 mutagenesis Methods 0.000 description 1
- 231100000350 mutagenesis Toxicity 0.000 description 1
- 230000017066 negative regulation of growth Effects 0.000 description 1
- 229960003512 nicotinic acid Drugs 0.000 description 1
- 235000001968 nicotinic acid Nutrition 0.000 description 1
- 239000011664 nicotinic acid Substances 0.000 description 1
- 229920001220 nitrocellulos Polymers 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 235000018343 nutrient deficiency Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 229940126578 oral vaccine Drugs 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 238000005192 partition Methods 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 235000020030 perry Nutrition 0.000 description 1
- 229940066827 pertussis vaccine Drugs 0.000 description 1
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 238000002264 polyacrylamide gel electrophoresis Methods 0.000 description 1
- 229920005862 polyol Polymers 0.000 description 1
- 230000004481 post-translational protein modification Effects 0.000 description 1
- 230000003389 potentiating effect Effects 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 229960000856 protein c Drugs 0.000 description 1
- 108060006633 protein kinase Proteins 0.000 description 1
- 230000002797 proteolythic effect Effects 0.000 description 1
- 150000003212 purines Chemical class 0.000 description 1
- 102000005962 receptors Human genes 0.000 description 1
- 238000010188 recombinant method Methods 0.000 description 1
- 238000005215 recombination Methods 0.000 description 1
- 230000022532 regulation of transcription, DNA-dependent Effects 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 230000003252 repetitive effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 238000010839 reverse transcription Methods 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 206010039073 rheumatoid arthritis Diseases 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 102200082950 rs35685286 Human genes 0.000 description 1
- 239000006152 selective media Substances 0.000 description 1
- 229940007046 shigella dysenteriae Drugs 0.000 description 1
- 238000002741 site-directed mutagenesis Methods 0.000 description 1
- 210000000813 small intestine Anatomy 0.000 description 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
- 235000017557 sodium bicarbonate Nutrition 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 229940031000 streptococcus pneumoniae Drugs 0.000 description 1
- 238000007920 subcutaneous administration Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000008093 supporting effect Effects 0.000 description 1
- 229940124597 therapeutic agent Drugs 0.000 description 1
- 235000019157 thiamine Nutrition 0.000 description 1
- KYMBYSLLVAOCFI-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SCN1CC1=CN=C(C)N=C1N KYMBYSLLVAOCFI-UHFFFAOYSA-N 0.000 description 1
- 229960003495 thiamine Drugs 0.000 description 1
- 239000011721 thiamine Substances 0.000 description 1
- 150000003573 thiols Chemical class 0.000 description 1
- 238000002627 tracheal intubation Methods 0.000 description 1
- 230000002103 transcriptional effect Effects 0.000 description 1
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
- 230000007501 viral attachment Effects 0.000 description 1
- 230000029812 viral genome replication Effects 0.000 description 1
- 230000001018 virulence Effects 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 229940051021 yellow-fever virus Drugs 0.000 description 1
- 229940098232 yersinia enterocolitica Drugs 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/002—Protozoa antigens
- A61K39/015—Hemosporidia antigens, e.g. Plasmodium antigens
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/02—Bacterial antigens
- A61K39/025—Enterobacteriales, e.g. Enterobacter
- A61K39/0275—Salmonella
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
- C07K14/245—Escherichia (G)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/44—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from protozoa
- C07K14/445—Plasmodium
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
- C12N15/68—Stabilisation of the vector
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/93—Ligases (6)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
- A61K2039/52—Bacterial cells; Fungal cells; Protozoal cells
- A61K2039/522—Bacterial cells; Fungal cells; Protozoal cells avirulent or attenuated
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
- A61K2039/52—Bacterial cells; Fungal cells; Protozoal cells
- A61K2039/523—Bacterial cells; Fungal cells; Protozoal cells expressing foreign proteins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Tropical Medicine & Parasitology (AREA)
- Mycology (AREA)
- Animal Behavior & Ethology (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Pharmacology & Pharmacy (AREA)
- Immunology (AREA)
- Epidemiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Toxicology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oncology (AREA)
- Communicable Diseases (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Ultra Sonic Daignosis Equipment (AREA)
- Measurement And Recording Of Electrical Phenomena And Electrical Characteristics Of The Living Body (AREA)
Claims (11)
1. Vektor, kännetecknad av att den innehäller en purA-gen som kodar för adenylsuccinatsynte-5 tas, men inte innehäller en antibiotresistansdeterminant, varvid vektorn är exempelvis en plasmid med ett lagt antal kopior eller en plasmid med ett högt antal kopior, vilken innehäller en purA-gen som reglerats att uttryckas reduce-rat.
2. Vektor enligt patentkrav 1, känne tecknad av att den dessutom innehäller en nukleo-tidsekvens, som kodar för minst en annan antigen eller ett fragment därav.
3. Vektor enligt patentkrav 2, kanne-15tecknad av att antigenen är en virusantigen som valts ur gruppen som bildas av humant immundefektvirus (typerna I och II), humant T-lymfocytvirus (typerna I, II och III), respiratory syncytial (RS)-virus, hepatit A, he-patit B, hepatit C, icke-A- och icke-B-hepatitvirus, her-20 pes simplex-virus (typerna I och II), cytomegalovirus, in-fluensavirus, parainfluensavirus, poliovirus, rotavirus, coronavirus, rubellavirus, mässlingsvirus, vattkoppsvirus, Epstein Barr-virus, adenovirus, papillomvirus och gula fe-<_ bern-virus, en bakterieantigen som valts ur gruppen som 25 bildas av Haemophilus influenzae, Escherichia cold, Neisseria meningitidis, Streptococcus pneumoniae, Streptococcus pyogenes, Branhamella catarrhalis, Vibrio cholerae, Corynebacterium diphteriae, Chlamydia trachomatis, Neisseria gonorrhea, Bordetella pertussis, Pseudomonas aeruqino-,., 30 sa, Staphylococcus aureus, Klebsiella pneumoniae och Clo stridium tetani, en svamp- eller parasitantigen som här-stammar frän en patogen av ett varmblodigt djur eller en människa. 111013
4. Vektor enligt patentkrav 1, k ä n n e -tecknad av att den är pPX3005 (ATCC 68451), pPX3006 (ATCC 68452), pPX3009 (ATCC 68612), pPX3010 (ATCC 68453) eller den i figur 6B visade pPX3007.
5 5. Förfarande för framställning av en värdcell innehällande en vektor enligt nägot av patentkraven 1-4, kännetecknat av att nämnda vektor införs i nämnda värdcell.
6. Förfarande för framställning av en vaccinkompo-10 sition som omfattar en försvagad bakterie som innehaller en stabil vektor, som innehaller en heterolog purA-qen som kodar för adenylsuccinatsyntetas, men inte innehaller en antibiotresistansdeterminant, kännetecknat av att nämnda vektor införs i den försvagade bakterien, som 15 därefter blandas med ett fysiologiskt godtagbart bärarämne och valbart med en adjuvant, varvid vektorn är exempelvis en plasmid med ett lägt antal kopior eller en plasmid med ett högt antal kopior, vilken innehaller en purA-gen som reglerats att uttryckas reducerat. 20
7. Förfarande enligt patentkrav 6, kän netecknat av att bakterien uppvisar en purA-kromosomal genmutation och valbart en kromosomal mutation i en eller flera gener, som är funktionella i bio-syntesen för aromatiska föreningar. \ 25
8. Förfarande enligt patentkrav 6, kän netecknat av att genens produkt är en antigen för ett virus, en bakterie, svamp eller parasit, som är en patogen av ett varmblodigt djur eller en människa.
9. Förfarande enligt patentkrav 6, k ä n -30netecknat av att bakterien är en i tarmen ;·· inträngande bakterie av släktet Salmonella-, Shigella-, Yersinia-, Escherichia-, Vibrio- eller Campylobacter.
10. Förfarande enligt nägot av patentkraven 6-9, kännetecknat av att vektorn innehaller 35 en gen som kodar för en antigen, som är en epitop av ett malariecirkumsporozoitprotein och som uttrycks som en del 111013 av fusionsproteinet, som omfattar epitopen av cirkumsporo-zoitproteinet och en B-underenhet av värmelabilt entero-toxin av E. coli eller ett delprotein därav, som i före-ning med epitopen av cirkumsporozoitproteinet ästadkommer 5 ett immunoaktivt fusionsprotein.
11. Förfarande för vai och upprätthällande av bak-terietransformanter av intresse genom att använda icke-antibiotiska selektionsmedel, kännetecknat av att en vektor enligt nägot av patentkraven 1-4 införs i 10 vardbakterier, som saknar purA-genen, bakterierna odlas selektivt i ett odlingssubstrat som saknar adenin och i substratet växande bakteriekolonier innehallande purÄ-plasmid väljs. t
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US69570691A | 1991-05-03 | 1991-05-03 | |
| US69570691 | 1991-05-03 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| FI921940A0 FI921940A0 (fi) | 1992-04-30 |
| FI921940A FI921940A (fi) | 1992-11-04 |
| FI111013B true FI111013B (sv) | 2003-05-15 |
Family
ID=24794162
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| FI921940A FI111013B (sv) | 1991-05-03 | 1992-04-30 | Stabila purA-vektorer och deras användning |
Country Status (16)
| Country | Link |
|---|---|
| US (2) | US5919663A (sv) |
| EP (1) | EP0512260B1 (sv) |
| JP (1) | JP3320095B2 (sv) |
| KR (1) | KR100242798B1 (sv) |
| AT (1) | ATE202800T1 (sv) |
| AU (1) | AU654347B2 (sv) |
| CA (1) | CA2067862C (sv) |
| DE (1) | DE69231907T2 (sv) |
| DK (1) | DK0512260T3 (sv) |
| ES (1) | ES2160573T3 (sv) |
| FI (1) | FI111013B (sv) |
| GR (1) | GR3036487T3 (sv) |
| IL (1) | IL101751A0 (sv) |
| NO (1) | NO314043B1 (sv) |
| PT (1) | PT512260E (sv) |
| TW (1) | TW201794B (sv) |
Families Citing this family (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2738842B1 (fr) * | 1995-09-15 | 1997-10-31 | Rhone Poulenc Rorer Sa | Molecule d'adn circulaire a origine de replication conditionnelle, leur procede de preparation et leur utilisation en therapie genique |
| US7279313B2 (en) * | 1995-09-15 | 2007-10-09 | Centelion | Circular DNA molecule having a conditional origin of replication, process for their preparation and their use in gene therapy |
| CA2264677A1 (en) * | 1996-09-04 | 1998-03-12 | Basf Aktiengesellschaft | Adenylosuccinate synthetase |
| US6010705A (en) * | 1997-04-11 | 2000-01-04 | Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College | Attenuated, invasive vaccines against fish pathogens |
| WO1998046725A2 (en) * | 1997-04-11 | 1998-10-22 | Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College | Attenuated, invasive vaccines against fish pathogens |
| WO2000034498A1 (en) * | 1998-12-04 | 2000-06-15 | University Of Manitoba | Two-step immunization procedure against chlamydia infection |
| KR100427587B1 (ko) * | 2001-01-12 | 2004-04-27 | 주식회사 바이오리더스 | 신규 ｄ-글루탐산 합성효소 유전자 ｄｎａ 및 이를 이용한 항생제 비의존성 벡터 |
| FI116851B (sv) * | 2001-05-03 | 2006-03-15 | Fit Biotech Oyj Plc | Expressionsvektor, dess användningar och förfarande för framställning därav samt produkter som innehåller denna |
| US20040009937A1 (en) * | 2002-01-31 | 2004-01-15 | Wei Chen | Methods and composition for delivering nucleic acids and/or proteins to the respiratory system |
| WO2003063786A2 (en) * | 2002-01-31 | 2003-08-07 | Symbigene, Inc. | Methods and composition for delivering nucleic acids and/or proteins to the respiratory system |
| CN1276076C (zh) * | 2004-12-14 | 2006-09-20 | 华东理工大学 | 一种鳗弧菌野生毒株的无标记基因缺失减毒突变株及其应用 |
| WO2008141226A2 (en) * | 2007-05-10 | 2008-11-20 | The Arizona Board Of Regents For And On Behalf Of Arizona State University | Recombinant bacteria comprising vectors for expression of nucleic acid sequences encoding antigens |
| EP2150616A4 (en) | 2007-05-10 | 2011-07-27 | Univ Arizona | REGULATED ANTIGEN SYNTHESIS AND / OR REGULATED ATTENUATION FOR IMPROVING VACCINATE IMMUNOGENICITY AND / OR SAFETY |
| US8465755B2 (en) | 2007-10-05 | 2013-06-18 | The Arizona Board Of Regents For And On Behalf Of Arizona State University | Recombinant bacterium capable of eliciting an immune response against enteric pathogens |
| WO2010045620A1 (en) * | 2008-10-17 | 2010-04-22 | The Arizona Board Of Regents For And On Behalf Of Arizona State University | Recombinant bacterium capable of eliciting an immune response against streptococcus pneumoniae |
| US9163219B2 (en) * | 2009-04-14 | 2015-10-20 | Arizona Board Of Regents On Behalf Of Arizona State University | Single expression vector for generation of a virus with a segmented genome |
| GB2483595B (en) | 2009-05-22 | 2017-03-29 | The Arizona Board Of Regents For And On Behalf Of Arizona State Univ | Recombinant bacterium and methods of antigen and nucleic acid delivery |
| US9045742B2 (en) * | 2009-05-29 | 2015-06-02 | The Arizona Board Of Regents For And On Behalf Of Arizona State University | Recombinant Edwardsiella bacterium |
| US9062297B2 (en) | 2010-01-13 | 2015-06-23 | The Arizona Board Of Regents For And On Behalf Of Arizona State University | Yersinia pestis vaccine |
| WO2011091291A1 (en) | 2010-01-22 | 2011-07-28 | The Arizona Board Of Regents For And On Behalf Of Arizona State University | BACTERIUM COMPRISING A REGULATED rfaH NUCLEIC ACID |
| US9598697B2 (en) | 2010-05-28 | 2017-03-21 | The Arizona Board Of Regents For And On Behalf Of Arizona State University | Recombinant bacterium to decrease tumor growth |
| US20130266983A1 (en) * | 2010-12-14 | 2013-10-10 | The Regents Of The University Of California | Creation of super-stable ColE1 plasmids by duplication of SL1-4 sequence and point mutations. |
| US9303264B2 (en) | 2012-05-18 | 2016-04-05 | The Arizona Board Of Regents For And On Behalf Of Arizona State University | Photosynthetic microorganisms expressing thermostable lipase |
| US9580718B2 (en) | 2013-06-17 | 2017-02-28 | Arizona Board Of Regents On Behalf Of Arizona State University | Attenuated live bacteria with increased acid resistance and methods of use thereof |
| KR102006976B1 (ko) * | 2019-02-26 | 2019-08-06 | 씨제이제일제당 주식회사 | 신규 프로모터 및 이를 이용한 퓨린 뉴클레오티드 제조방법 |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0025118A1 (de) * | 1979-08-18 | 1981-03-18 | Riedlinger, Rainer, Dr.-Ing. | Anordnung zur akustischen Wiedergabe von Signalen, die mittels eines rechten und eines linken Stereo-Kanals darstellbar sind |
| US4837151A (en) * | 1980-05-19 | 1989-06-06 | The Board Of Trustees Of The Leland Stanford Junior University, Stanford University | Live vaccines comprising two mutations and foreign antigen |
| US4550081A (en) * | 1980-05-19 | 1985-10-29 | The Board Of Trustees Of The Leland Stanford Jr. University | Non-reverting salmonella |
| US4735801A (en) * | 1982-09-07 | 1988-04-05 | Board Of Trustees Of Leland Stanford Jr. University | Novel non-reverting salmonella live vaccines |
| DK410783D0 (da) * | 1982-09-16 | 1983-09-09 | Benzon A Salfred | Fremgangsmade til stabilisering af plasmider |
| EP0251579A3 (en) * | 1986-06-24 | 1989-03-22 | Enterovax Research Pty. Ltd. | Non-antibiotic marker system |
| DK408687A (da) * | 1986-08-05 | 1988-02-06 | Transgene Sa | Fremgangsmaade til stabilisering af et plasmid |
| JPS6427477A (en) * | 1987-04-01 | 1989-01-30 | Takeda Chemical Industries Ltd | Dna and use thereof |
| US5112749A (en) * | 1987-10-02 | 1992-05-12 | Praxis Biologics, Inc. | Vaccines for the malaria circumsporozoite protein |
| GB8730037D0 (en) * | 1987-12-23 | 1988-02-03 | Wellcome Found | Vaccines |
| WO1990012086A1 (en) * | 1989-03-31 | 1990-10-18 | Washington University | Bordetella vaccines |
| DE69132058D1 (de) * | 1990-12-18 | 2000-04-20 | Gen Hospital Corp | Verbesserte impfstoffe |
-
1992
- 1992-02-11 TW TW081100950A patent/TW201794B/zh active
- 1992-04-06 AT AT92105887T patent/ATE202800T1/de not_active IP Right Cessation
- 1992-04-06 PT PT92105887T patent/PT512260E/pt unknown
- 1992-04-06 DK DK92105887T patent/DK0512260T3/da active
- 1992-04-06 DE DE69231907T patent/DE69231907T2/de not_active Expired - Fee Related
- 1992-04-06 EP EP92105887A patent/EP0512260B1/en not_active Expired - Lifetime
- 1992-04-06 ES ES92105887T patent/ES2160573T3/es not_active Expired - Lifetime
- 1992-04-28 JP JP13437592A patent/JP3320095B2/ja not_active Expired - Fee Related
- 1992-04-30 FI FI921940A patent/FI111013B/sv not_active IP Right Cessation
- 1992-04-30 IL IL101751A patent/IL101751A0/xx not_active IP Right Cessation
- 1992-04-30 NO NO19921729A patent/NO314043B1/no unknown
- 1992-05-01 AU AU15959/92A patent/AU654347B2/en not_active Ceased
- 1992-05-01 CA CA002067862A patent/CA2067862C/en not_active Expired - Fee Related
- 1992-05-02 KR KR1019920007559A patent/KR100242798B1/ko not_active IP Right Cessation
-
1995
- 1995-01-30 US US08/380,297 patent/US5919663A/en not_active Expired - Fee Related
- 1995-05-24 US US08/448,907 patent/US5961983A/en not_active Expired - Fee Related
-
2001
- 2001-08-31 GR GR20010401346T patent/GR3036487T3/el not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| NO921729L (no) | 1992-11-04 |
| US5919663A (en) | 1999-07-06 |
| FI921940A (fi) | 1992-11-04 |
| IL101751A0 (en) | 1992-12-30 |
| TW201794B (sv) | 1993-03-11 |
| PT512260E (pt) | 2001-12-28 |
| CA2067862C (en) | 2003-12-30 |
| EP0512260B1 (en) | 2001-07-04 |
| KR100242798B1 (ko) | 2000-02-01 |
| AU654347B2 (en) | 1994-11-03 |
| KR920021701A (ko) | 1992-12-18 |
| AU1595992A (en) | 1992-11-05 |
| DK0512260T3 (da) | 2001-09-17 |
| NO921729D0 (no) | 1992-04-30 |
| GR3036487T3 (en) | 2001-11-30 |
| EP0512260A3 (en) | 1993-07-28 |
| DE69231907T2 (de) | 2001-10-18 |
| ES2160573T3 (es) | 2001-11-16 |
| JP3320095B2 (ja) | 2002-09-03 |
| NO314043B1 (no) | 2003-01-20 |
| JPH05192161A (ja) | 1993-08-03 |
| US5961983A (en) | 1999-10-05 |
| ATE202800T1 (de) | 2001-07-15 |
| FI921940A0 (fi) | 1992-04-30 |
| DE69231907D1 (de) | 2001-08-09 |
| CA2067862A1 (en) | 1992-11-04 |
| EP0512260A2 (en) | 1992-11-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| FI111013B (sv) | Stabila purA-vektorer och deras användning | |
| FI110008B (sv) | Förfarande för framställning av en försvagad bakterie och vaccin som innehåller denna | |
| Garmory et al. | Salmonella vaccines for use in humans: present and future perspectives | |
| US5424065A (en) | Vaccines containing avirulent phop-type microorganisms | |
| EP1076566B1 (en) | Attenuated mutants of salmonella which constitutively express the vi antigen | |
| EP0563311B1 (en) | Improved vaccines | |
| JP4583607B2 (ja) | 感染症の治療のための弱毒化微生物 | |
| JPH11507532A (ja) | 環境に制限される生存能を有する組換え細菌システム | |
| JPH04502402A (ja) | 組み換えフラジエリンのワクチン | |
| US8475810B2 (en) | Attenuated Salmonella enterica serovar paratyphi a and uses thereof | |
| US8137930B2 (en) | Attenuated Salmonella enterica serovar paratyphi A and uses thereof | |
| EP0465561A1 (en) | Avirulent microbes and uses therefor | |
| WO1991018092A1 (en) | Acid-tolerant salmonella typhi, double aro mutants thereof and use as an oral vaccine for typhoid fever | |
| AU653362B2 (en) | Insertion of DNA by modified transposons | |
| PL171476B1 (en) | Method of obtaining attenuated salmonella bacterium |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MA | Patent expired |