FI110323B - Rekombinantkonstruktion för ökning av genexpression hos växter - Google Patents
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- FI110323B FI110323B FI972325A FI972325A FI110323B FI 110323 B FI110323 B FI 110323B FI 972325 A FI972325 A FI 972325A FI 972325 A FI972325 A FI 972325A FI 110323 B FI110323 B FI 110323B
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- gene
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- recombinant dna
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
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- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Fertilizers (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Claims (9)
1. Rekombinant DNA-sekvens, kännetecnad därav, att den innehäller: (a) en transkriptional promotor; 5 (b) en i en växt uttryckande, frän virus härstammande replikasgen i sense-orientation, som i ligger i 3’-riktning frän den transkriptionala promotom; (c) en eller flera i en växt superuttryckande gensekvenser i antisense-orientering, som ligger närä replikasgenen; (d) en frän virus härstammande eukaryotisk, växtspesifik, undergenomisk 10 promotorsekvens, vilken promotorsekvens star i växelverkan med replikasgenen, ligerad med den superuttryckta gensekvensen; sä, att replikasgenen, den superuttryckta gensekvensen och den undergenomiska promotorsekvensen läses under verkan av nämnda transkriptionala promotor, varvid erhälles ett primärt transkript, som kan läses medelst det uttryckta replikaset och nämnda 15 undergenomiska promotor producerar ett sekundärt transkirpt.
2. Rekombinant DNA-sekvens enligt patentkravet 1, kännetecknad därav, att replikasgenens sekvens är i en orientering, i vilken transkriptionen producerar ett primärt plus-sense RNA-transkript.
: ·.: 3. Rekombinant DNA-sekvens enligt patentkravet 1, kännetecknad därav, att . ' : gensekvensen, som superuttrycks, och den undergenomiska promotorsekvensen är i en • / · j orientering, i vilken transkriptionen producerar ett primärt minus-sense RNA-transkript. :. :23
4. Rekombinant DNA-sekvens enligt patentkravet 1, kännetecknad därav, att den transkriptionala promotom är en eukaryotisk växtvirusspesifik promotor.
... * 5. Rekombinant DNA-sekvens enligt patentkravet 1, kännetecknad därav, att : : ’: replikasgenen är en eukaryotisk växtspesifik replikasgen av växtvirus. : ’ 3(5 ,:.
6. Rekombinant DNA-sekvens enligt patentkravet 1 eller 3, kännetecknad därav, att . ·. : gensekvensen, som uttrycks, kodar för en önskad polypeptidsprodukt, som kan vara ett antibiotikum, toxin, hormon, enzym eller ett mikrobial- eller djurprotein, som päverkar 16 110323 virusinfektion eller modifierar sädana egenskaper som fenotyp, sterilitet, salttolerans, mottaglighet för virus, torrhetstolerans, surhet eller växtens färg.
7. Rekombinant DNA-sekvens enligt patentkravet 1, kännetecknad därav, att den 5 dessutom innehäller en gen, som kodar för en selektiv markör, som ger antibiotikaresistens, herbicidresistens eller ändring av färg, eller kodar för polypeptider, som reagerar med ett substrat under produktion av en detekterande signal.
8. Rekombinant DNA-sekvens enligt patentkravet 1, kännetecknad därav, att den 10 dessutom innehäller tranlationsökande nukleotidsekvenser, vilka ligger mellan transkriptionens ingängsposition för den undergenomiska promotom och 5’-ändan av den superuttryckta gensekvensen.
9. Växtcell, kännetecknad därav, att den är transformerad med en rekombinant DNA-15 sekvens enligt nägon av patentkraven 1-8. • · • · ·
Priority Applications (8)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FI972325A FI110323B (sv) | 1997-06-02 | 1997-06-02 | Rekombinantkonstruktion för ökning av genexpression hos växter |
| CA002292897A CA2292897C (en) | 1997-06-02 | 1998-05-28 | Recombinant construct for enhancement of gene expression in plants |
| EP98922831A EP0988387B9 (en) | 1997-06-02 | 1998-05-28 | Recombinant construct for enhancement of gene expression in plants |
| AU75329/98A AU7532998A (en) | 1997-06-02 | 1998-05-28 | Recombinant construct for enhancement of gene expression in plants |
| US09/445,092 US6573427B1 (en) | 1997-06-02 | 1998-05-28 | Recombinant construct for enhancement of gene expression in plants |
| PCT/FI1998/000445 WO1998055636A1 (en) | 1997-06-02 | 1998-05-28 | Recombinant construct for enhancement of gene expression in plants |
| DE69836136T DE69836136T2 (de) | 1997-06-02 | 1998-05-28 | Rekombinantes konstrukt für eine erhöhte expression von genen in pflanzen. |
| AT98922831T ATE342366T1 (de) | 1997-06-02 | 1998-05-28 | Rekombinantes konstrukt für eine erhöhte expression von genen in pflanzen. |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FI972325 | 1997-06-02 | ||
| FI972325A FI110323B (sv) | 1997-06-02 | 1997-06-02 | Rekombinantkonstruktion för ökning av genexpression hos växter |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| FI972325A0 FI972325A0 (fi) | 1997-06-02 |
| FI972325A FI972325A (sv) | 1998-12-03 |
| FI110323B true FI110323B (sv) | 2002-12-31 |
Family
ID=8548956
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| FI972325A FI110323B (sv) | 1997-06-02 | 1997-06-02 | Rekombinantkonstruktion för ökning av genexpression hos växter |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US6573427B1 (sv) |
| EP (1) | EP0988387B9 (sv) |
| AT (1) | ATE342366T1 (sv) |
| AU (1) | AU7532998A (sv) |
| CA (1) | CA2292897C (sv) |
| DE (1) | DE69836136T2 (sv) |
| FI (1) | FI110323B (sv) |
| WO (1) | WO1998055636A1 (sv) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7235716B2 (en) | 1997-06-03 | 2007-06-26 | Chromatin, Inc. | Plant centromere compositions |
| US7227057B2 (en) | 1997-06-03 | 2007-06-05 | Chromatin, Inc. | Plant centromere compositions |
| US7193128B2 (en) | 1997-06-03 | 2007-03-20 | Chromatin, Inc. | Methods for generating or increasing revenues from crops |
| US7119250B2 (en) | 1997-06-03 | 2006-10-10 | The University Of Chicago | Plant centromere compositions |
| EP1175144A4 (en) * | 1998-12-22 | 2002-10-30 | Thompson Boyce Plant Res | ORAL ADMINISTRATION IMMUNOGENIC BACTERIAL ENTEROTOXINS EXPRESSED IN TRANSGENIC PLANTS |
| US7989202B1 (en) | 1999-03-18 | 2011-08-02 | The University Of Chicago | Plant centromere compositions |
| US7348179B2 (en) | 2001-04-17 | 2008-03-25 | Icon Genetics, Inc. | IRES enabled gene trapping in plants |
| WO2002101006A2 (en) * | 2001-06-08 | 2002-12-19 | Icon Genetics, Inc. | Production of proteins in plants |
| US8729341B2 (en) * | 2004-02-23 | 2014-05-20 | University Of Chicago | Plants modified with mini-chromosomes |
| EP1929019A2 (en) * | 2005-09-08 | 2008-06-11 | Chromatin, Inc. | Plants modified with mini-chromosomes |
| WO2008112972A2 (en) * | 2007-03-15 | 2008-09-18 | Chromatin, Inc. | Centromere sequences and minichromosomes |
| CA2767724A1 (en) | 2009-07-23 | 2011-01-27 | Chromatin, Inc. | Sorghum centromere sequences and minichromosomes |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5316931A (en) * | 1988-02-26 | 1994-05-31 | Biosource Genetics Corp. | Plant viral vectors having heterologous subgenomic promoters for systemic expression of foreign genes |
| US5977438A (en) | 1988-02-26 | 1999-11-02 | Biosource Technologies, Inc. | Production of peptides in plants as viral coat protein fusions |
| US5217879A (en) * | 1989-01-12 | 1993-06-08 | Washington University | Infectious Sindbis virus vectors |
| US5633441A (en) * | 1989-08-04 | 1997-05-27 | Plant Genetic Systems, N.V. | Plants with genetic female sterility |
| WO1991013994A1 (en) * | 1990-03-13 | 1991-09-19 | Commonwealth Scientific And Industrial Research Organisation | Gene expression |
| ES2171398T3 (es) | 1991-08-01 | 2002-09-16 | Large Scale Biology Corp | Acidos nucleicos viricos vegetales recombinantes. |
| DE69232438T2 (de) | 1991-08-07 | 2002-10-10 | W. French Anderson | Interne ribosom eintrittsstellen enthaltene retrovirale vektoren |
| DE69333529T2 (de) * | 1992-03-31 | 2004-10-14 | Kanebo, Ltd. | Pflanzenvirus-vektor, plasmid, methode der expression fremder gene und methode zur gewinnung obiger genprodukte |
| AU668387B2 (en) * | 1992-04-28 | 1996-05-02 | Nihon Nohyaku Co., Ltd. | Process for production of exogenous gene or its product in plant cells |
| DE4315109A1 (de) * | 1993-05-06 | 1994-11-10 | Inst Pflanzengenetik & Kultur | Verfahren und Vektorkonstrukt zur Expressionssteigerung von Transgenen |
| SE9401091D0 (sv) | 1994-03-31 | 1994-03-31 | Bioption Ab | Alphavirus cDNA vectors |
| US5437706A (en) | 1994-06-10 | 1995-08-01 | Borealis Technical Incorporated Limited | Method for operating a blast furnace |
| US5633447A (en) | 1994-08-25 | 1997-05-27 | Mycogen Plant Science, Inc. | Plant tissue comprising a subgenomic promoter |
-
1997
- 1997-06-02 FI FI972325A patent/FI110323B/sv not_active IP Right Cessation
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1998
- 1998-05-28 AU AU75329/98A patent/AU7532998A/en not_active Abandoned
- 1998-05-28 WO PCT/FI1998/000445 patent/WO1998055636A1/en active IP Right Grant
- 1998-05-28 EP EP98922831A patent/EP0988387B9/en not_active Expired - Lifetime
- 1998-05-28 CA CA002292897A patent/CA2292897C/en not_active Expired - Fee Related
- 1998-05-28 AT AT98922831T patent/ATE342366T1/de active
- 1998-05-28 DE DE69836136T patent/DE69836136T2/de not_active Expired - Lifetime
- 1998-05-28 US US09/445,092 patent/US6573427B1/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| FI972325A0 (fi) | 1997-06-02 |
| CA2292897A1 (en) | 1998-12-10 |
| WO1998055636A1 (en) | 1998-12-10 |
| US6573427B1 (en) | 2003-06-03 |
| EP0988387A1 (en) | 2000-03-29 |
| DE69836136D1 (de) | 2006-11-23 |
| AU7532998A (en) | 1998-12-21 |
| CA2292897C (en) | 2007-05-01 |
| EP0988387B1 (en) | 2006-10-11 |
| FI972325A (sv) | 1998-12-03 |
| DE69836136T2 (de) | 2007-03-15 |
| ATE342366T1 (de) | 2006-11-15 |
| EP0988387B9 (en) | 2007-02-28 |
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