ES8802545A1 - Un metodo para determinar prolil 4-hidroxilasa humana por inmunoensayo enzimatico o radioinmunoensayo - Google Patents

Un metodo para determinar prolil 4-hidroxilasa humana por inmunoensayo enzimatico o radioinmunoensayo

Info

Publication number
ES8802545A1
ES8802545A1 ES552695A ES552695A ES8802545A1 ES 8802545 A1 ES8802545 A1 ES 8802545A1 ES 552695 A ES552695 A ES 552695A ES 552695 A ES552695 A ES 552695A ES 8802545 A1 ES8802545 A1 ES 8802545A1
Authority
ES
Spain
Prior art keywords
hydroxylase
human prolyl
immunoassay
determining human
prolyl
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES552695A
Other languages
English (en)
Other versions
ES552695A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fuji Yakuhin Kogyo KK
Original Assignee
Fuji Yakuhin Kogyo KK
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from JP60042686A external-priority patent/JPH0672892B2/ja
Priority claimed from JP60179357A external-priority patent/JPH0672893B2/ja
Application filed by Fuji Yakuhin Kogyo KK filed Critical Fuji Yakuhin Kogyo KK
Publication of ES552695A0 publication Critical patent/ES552695A0/es
Publication of ES8802545A1 publication Critical patent/ES8802545A1/es
Expired legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N31/00—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/811—Test for named disease, body condition or organ function
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/82—Hepatitis associated antigens and antibodies

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

METODO PARA DETERMINAR PROLIL 4-HIDROXILASA HUMANA POR INMUNOENSAYO ENZIMATICO O RADIOINMUNOENSAYO, EN EL QUE: A) SE REVISTEN BOLAS DE POLIESTERENOMEDIANTE INMERSION EN UNA DISOLUCION DE UN ANTICUERPO MONOCLONAL Y/O UN ANTICUERPO POLICLONAL ANTI-HUMANO PROLIL 4-HIDROXILASA (ANTI-HPH) EN UN TAMPON FOSFATO, DE PH 7,5, CON AZIDA DE SODIO, MAS 24 HORAS, A UNOS 40GC, Y SE LAVAN PARA OBTENER BOLAS DE POLIESTIRENO CON EL ANTICUERPO REVESTIDO; B) SE INCUBAN DICHAS BOLAS CON UNA SOLUCION DE LA MUESTRA HPH A ANALIZAR A UNOS 30GC DE 1 A 4 HORAS CON AGITACION, SE SEPARAN LAS BOLAS QUE LLEVAN ACOPLADO EL ANTICUERPO Y LA HPH Y SE LAVAN; C) SE INCUBAN LAS BOLAS DE LA ETAPA ANTERIOR CON UNA SOLUCION DE UN ANTICUERPO ANTI-HPH MARCADO ENZIMATICAMENTE O CON UN ELEMENTO RADIOACTIVO, A UNOS 40GC-30GC, DE 1 A 12 HORAS, SE SEPARAN Y LAVAN LAS BOLAS Y SE ANALIZAN LA CANTIDAD DE HPH ADHERIDA. TIENE APLICACION MEDICA PARA LA DIAGNOSIS DE ENFERMEDADES HEPATICAS.
ES552695A 1985-03-06 1986-03-05 Un metodo para determinar prolil 4-hidroxilasa humana por inmunoensayo enzimatico o radioinmunoensayo Expired ES8802545A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP60042686A JPH0672892B2 (ja) 1985-03-06 1985-03-06 ヒト血中のヒトプロリルヒドロキシラーゼの酵素免疫定量法
JP60179357A JPH0672893B2 (ja) 1985-08-16 1985-08-16 ヒト血中のヒトプロリルヒドロキシラーゼの放射性免疫学的測定法による定量法

Publications (2)

Publication Number Publication Date
ES552695A0 ES552695A0 (es) 1988-06-16
ES8802545A1 true ES8802545A1 (es) 1988-06-16

Family

ID=26382408

Family Applications (1)

Application Number Title Priority Date Filing Date
ES552695A Expired ES8802545A1 (es) 1985-03-06 1986-03-05 Un metodo para determinar prolil 4-hidroxilasa humana por inmunoensayo enzimatico o radioinmunoensayo

Country Status (5)

Country Link
EP (1) EP0193935B1 (es)
KR (1) KR940002521B1 (es)
AU (1) AU590746B2 (es)
DE (1) DE3686068T2 (es)
ES (1) ES8802545A1 (es)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0672893B2 (ja) * 1985-08-16 1994-09-14 富士薬品工業株式会社 ヒト血中のヒトプロリルヒドロキシラーゼの放射性免疫学的測定法による定量法
WO2001068868A2 (en) * 2000-03-15 2001-09-20 Fibrogen, Inc. Alpha(iii) subunit of prolyl 4-hydroxylase
CN103224542B (zh) * 2013-04-17 2014-09-10 江苏澳格姆生物科技有限公司 一种用于常温下抗体活性保存的溶液及其制备方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4486530A (en) * 1980-08-04 1984-12-04 Hybritech Incorporated Immunometric assays using monoclonal antibodies
ES8307897A1 (es) * 1981-04-13 1983-08-01 Hoechst Co American Un metodo de determinar la presencia de un antigeno en un medio liquido sospechoso de contenerlo.
AU3217284A (en) * 1984-07-31 1986-02-25 Nagai, Y. Process for preparing human proline hydroxylase
EP0190350A1 (en) * 1984-07-31 1986-08-13 NAGAI, Yoshiki Process for preparing human proline hydroxylase

Also Published As

Publication number Publication date
ES552695A0 (es) 1988-06-16
DE3686068T2 (de) 1993-02-11
AU590746B2 (en) 1989-11-16
EP0193935B1 (en) 1992-07-22
KR860007545A (ko) 1986-10-13
KR940002521B1 (ko) 1994-03-25
AU5425386A (en) 1986-09-11
EP0193935A3 (en) 1987-12-02
EP0193935A2 (en) 1986-09-10
DE3686068D1 (de) 1992-08-27

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Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 20000403