ES8801376A1 - Un procedimiento para preparar una proteina hibrida - Google Patents
Un procedimiento para preparar una proteina hibridaInfo
- Publication number
- ES8801376A1 ES8801376A1 ES557103A ES557103A ES8801376A1 ES 8801376 A1 ES8801376 A1 ES 8801376A1 ES 557103 A ES557103 A ES 557103A ES 557103 A ES557103 A ES 557103A ES 8801376 A1 ES8801376 A1 ES 8801376A1
- Authority
- ES
- Spain
- Prior art keywords
- hybrid protein
- plasmid
- hybrid
- cell
- dna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
- C07K14/245—Escherichia (G)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
- C07K14/25—Shigella (G)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/28—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Vibrionaceae (F)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/34—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Corynebacterium (G)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/54—Interleukins [IL]
- C07K14/55—IL-2
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56966—Animal cells
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/55—Fusion polypeptide containing a fusion with a toxin, e.g. diphteria toxin
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
- C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Cell Biology (AREA)
- Physics & Mathematics (AREA)
- Toxicology (AREA)
- Pathology (AREA)
- Food Science & Technology (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Virology (AREA)
- Endocrinology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Plant Pathology (AREA)
- Botany (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Lubricants (AREA)
- Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
- Adhesives Or Adhesive Processes (AREA)
Abstract
PROCEDIMIENTO PARA PREPARAR UNA PROTEINA HIBRIDA. COMPRENDE: A) TRANSFORMAR LA CELULA HOSPEDANTE CON UN PLASMIDO; B) CULTIVAR LAS CELULAS TRANSFORMADAS ENTRE 20J Y 50JC; C) RECOGER LAS PROTEINAS HIBRIDAS DEL MEDIO DE CULTIVO DE B) POR METODOS CONVENCIONALES. EL PLASMIDO SE SELECCIONA ENTRE PUC7, PUC8 Y PUC9; CONTIENE UN GEN FUSIONADO DOTADO CON UNA PRIMERA SECUENCIA DE ADN QUE CODIFICA UNA PORCION DEL FRAGMENTO B DE LA MOLECULA DE TOXINA DE LA DIFTERIA, HACE PENETRAR A LA PROTEINA HIBRIDA EN LA MEMBRANA CITOPLASMATICA DE UNA CELULA Y FUSIONA UNA SEGUNDA SECUENCIA DE ADN QUE CODIFICA UNA PORCION DE UN LIGANDO POLIPEPTIDO EFICAZ. SE UTILIZA COMO AGENTES ANTIINMUNOLOGICOS.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US61819984A | 1984-06-07 | 1984-06-07 |
Publications (2)
Publication Number | Publication Date |
---|---|
ES8801376A1 true ES8801376A1 (es) | 1987-12-16 |
ES557103A0 ES557103A0 (es) | 1987-12-16 |
Family
ID=24476731
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES543938A Expired ES8708014A1 (es) | 1984-06-07 | 1985-06-05 | Un procedimiento para preparar un gen fusionado que codifica una proteina hibrida susceptible de marcado y empleo en diagnosis. |
ES557103A Expired ES8801376A1 (es) | 1984-06-07 | 1986-10-01 | Un procedimiento para preparar una proteina hibrida |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES543938A Expired ES8708014A1 (es) | 1984-06-07 | 1985-06-05 | Un procedimiento para preparar un gen fusionado que codifica una proteina hibrida susceptible de marcado y empleo en diagnosis. |
Country Status (11)
Country | Link |
---|---|
EP (1) | EP0185076B1 (es) |
JP (2) | JP2510984B2 (es) |
AT (1) | ATE79136T1 (es) |
AU (1) | AU592310B2 (es) |
CA (1) | CA1339359C (es) |
DE (1) | DE3586456T2 (es) |
ES (2) | ES8708014A1 (es) |
FI (1) | FI100253B (es) |
NO (1) | NO176807C (es) |
NZ (1) | NZ212312A (es) |
WO (1) | WO1986000090A1 (es) |
Families Citing this family (21)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU570762B2 (en) * | 1983-12-23 | 1988-03-24 | Australian National University, The | Cloning of cdna for il-3 |
US5668255A (en) * | 1984-06-07 | 1997-09-16 | Seragen, Inc. | Hybrid molecules having translocation region and cell-binding region |
AU577856B2 (en) * | 1985-01-17 | 1988-10-06 | Immunex Corporation | Cloning and characterization of the human interleukin 1 gene |
US4892827A (en) * | 1986-09-24 | 1990-01-09 | The United States Of America As Represented By The Department Of Health And Human Services | Recombinant pseudomonas exotoxins: construction of an active immunotoxin with low side effects |
US5132405A (en) * | 1987-05-21 | 1992-07-21 | Creative Biomolecules, Inc. | Biosynthetic antibody binding sites |
US5091513A (en) * | 1987-05-21 | 1992-02-25 | Creative Biomolecules, Inc. | Biosynthetic antibody binding sites |
DE3856559T2 (de) * | 1987-05-21 | 2004-04-29 | Micromet Ag | Multifunktionelle Proteine mit vorbestimmter Zielsetzung |
US5258498A (en) * | 1987-05-21 | 1993-11-02 | Creative Biomolecules, Inc. | Polypeptide linkers for production of biosynthetic proteins |
ATE87007T1 (de) * | 1987-05-29 | 1993-04-15 | Sagami Chem Res | Fusionsprotein, enthaltend lymphotoxin. |
JP2878341B2 (ja) * | 1989-03-03 | 1999-04-05 | 学校法人藤田学園 | 人工機能性ポリペプチド |
AU5355790A (en) * | 1989-04-19 | 1990-11-16 | Cetus Corporation | Multifunctional m-csf proteins and genes encoding therefor |
WO1991009871A1 (en) * | 1989-12-22 | 1991-07-11 | Seragen Incorporated | Hybrid molecules having translocation region and cell-binding region |
ES2155821T5 (es) * | 1990-03-02 | 2008-04-16 | Boston Medical Center Corporation | Toxinas quimericas mejoradas. |
US5621083A (en) * | 1991-11-04 | 1997-04-15 | Xoma Corporation | Immunotoxins comprising ribosome-inactivating proteins |
US5837491A (en) * | 1991-11-04 | 1998-11-17 | Xoma Corporation | Polynucleotides encoding gelonin sequences |
US6146850A (en) * | 1991-11-04 | 2000-11-14 | Xoma Corporation | Proteins encoding gelonin sequences |
WO1994025616A1 (en) * | 1993-04-28 | 1994-11-10 | Worcester Foundation For Experimental Biology | Cell-targeted lytic pore-forming agents |
US5777078A (en) * | 1993-04-28 | 1998-07-07 | Worcester Foundation For Experimental Biology | Triggered pore-forming agents |
US6635740B1 (en) | 1997-03-27 | 2003-10-21 | Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College | Ligand/lytic peptide compositions and methods of use |
AU8586298A (en) | 1997-07-25 | 1999-02-16 | University Of Massachusetts | Designed protein pores as components for biosensors |
US6680058B1 (en) | 1997-09-03 | 2004-01-20 | Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College | Compositions and methods for contraception in or sterilization of mammals |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU573529B2 (en) * | 1982-05-12 | 1988-06-16 | President And Fellows Of Harvard College | Hybrid proteins |
US4468382A (en) * | 1982-07-15 | 1984-08-28 | New England Medical Center, Inc. | Polypeptide-toxin hybrid protein |
-
1985
- 1985-06-05 NZ NZ212312A patent/NZ212312A/xx unknown
- 1985-06-05 ES ES543938A patent/ES8708014A1/es not_active Expired
- 1985-06-07 AU AU44916/85A patent/AU592310B2/en not_active Ceased
- 1985-06-07 WO PCT/US1985/001081 patent/WO1986000090A1/en active IP Right Grant
- 1985-06-07 DE DE8585903136T patent/DE3586456T2/de not_active Expired - Fee Related
- 1985-06-07 CA CA000483407A patent/CA1339359C/en not_active Expired - Fee Related
- 1985-06-07 AT AT85903136T patent/ATE79136T1/de not_active IP Right Cessation
- 1985-06-07 EP EP85903136A patent/EP0185076B1/en not_active Expired - Lifetime
- 1985-06-07 JP JP60502723A patent/JP2510984B2/ja not_active Expired - Lifetime
-
1986
- 1986-02-06 NO NO860421A patent/NO176807C/no not_active IP Right Cessation
- 1986-02-06 FI FI860541A patent/FI100253B/fi active IP Right Grant
- 1986-10-01 ES ES557103A patent/ES8801376A1/es not_active Expired
-
1993
- 1993-12-01 JP JP5302099A patent/JPH06205684A/ja active Pending
Also Published As
Publication number | Publication date |
---|---|
NO860421L (no) | 1986-02-06 |
JPH06205684A (ja) | 1994-07-26 |
JPS61502304A (ja) | 1986-10-16 |
AU592310B2 (en) | 1990-01-11 |
NO176807B (no) | 1995-02-20 |
NO176807C (no) | 1995-05-31 |
AU4491685A (en) | 1986-01-10 |
WO1986000090A1 (en) | 1986-01-03 |
EP0185076A4 (es) | 1987-11-09 |
ES543938A0 (es) | 1987-09-01 |
ATE79136T1 (de) | 1992-08-15 |
EP0185076B1 (en) | 1992-08-05 |
JP2510984B2 (ja) | 1996-06-26 |
FI860541A (fi) | 1986-02-06 |
EP0185076A1 (es) | 1986-06-25 |
CA1339359C (en) | 1997-08-26 |
ES557103A0 (es) | 1987-12-16 |
FI860541A0 (fi) | 1986-02-06 |
DE3586456D1 (de) | 1992-09-10 |
ES8708014A1 (es) | 1987-09-01 |
DE3586456T2 (de) | 1993-03-25 |
FI100253B (fi) | 1997-10-31 |
NZ212312A (en) | 1988-09-29 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FD1A | Patent lapsed |
Effective date: 20071029 |