ES8609478A1 - Un metodo para aumentar la velocidad de hibridacion entre dos segmentos polinucleotidicos complementarios en un medio acuoso. - Google Patents

Un metodo para aumentar la velocidad de hibridacion entre dos segmentos polinucleotidicos complementarios en un medio acuoso.

Info

Publication number
ES8609478A1
ES8609478A1 ES549043A ES549043A ES8609478A1 ES 8609478 A1 ES8609478 A1 ES 8609478A1 ES 549043 A ES549043 A ES 549043A ES 549043 A ES549043 A ES 549043A ES 8609478 A1 ES8609478 A1 ES 8609478A1
Authority
ES
Spain
Prior art keywords
nucleic acid
acid hybridization
anionic polymers
enhancement
polyacrylate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES549043A
Other languages
English (en)
Other versions
ES549043A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bayer Corp
Original Assignee
Miles Laboratories Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Miles Laboratories Inc filed Critical Miles Laboratories Inc
Publication of ES8609478A1 publication Critical patent/ES8609478A1/es
Publication of ES549043A0 publication Critical patent/ES549043A0/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6832—Enhancement of hybridisation reaction
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/803—Physical recovery methods, e.g. chromatography, grinding
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

METODO PARA DETERMINAR LA PRESENCIA DE UNA SECUENCIA POLINUCLEOTIDICA PARTICULAR EN UNA MUESTRA A ENSAYAR, QUE CONTIENE ACIDOS NUCLEICOS MONOCATENARIOS. LA MUESTRA A ENSAYAR SE COMBINA CON UNA SONDA POLINUCLEOTIDICA, CON UNA SECUENCIA DE BASES SUSTANCIALMENTE COMPLEMENTARIA DE LA SECUENCIA A DETERMINAR, PARA FORMAR UN MEDIO DE ENSAYO ACUOSO; PARA AUMENTAR LA VELOCIDAD DE HIBRIDACION ENTRE LA SONDA Y LA SECUENCIA A DETERMINAR, SE INCLUYE POLIACRILATO O POLIMETACRILATO EN EL CITADO MEDIO, Y LUEGO SE DETECTA LA FORMACION DE LA SONDA HIBRIDADA A LA SECUENCIA A DETERMINAR. EL POLIACRILATO SE USA EN SOLUCION DE 0,2-10% PESO/VOLUMEN Y EL POLIMETACRILATO EN SOLUCION DE 1,0 A 50% PESO/VOLUMEN Y AMBOS TIENEN UN PESO MOLECULAR DE 50.000 A 500.000 DALTON. LOS ACIDOS NUCLEICOS MONOCATENARIOS SE PUEDEN INMOVILIZAR EN NITROCELULOSA. LA TECNICA SE APLICA EN LA DETECCION E IDENTIFICACION DE AGENTES ETIOLOGICOS, DETERMINAR LA RESISTENCIA DE BACTERIAS O ANTIBIOTICOS Y EN LA DIAGNOSIS DE TRASTORNOS GENETICOS.
ES549043A 1984-11-19 1985-11-19 Un metodo para aumentar la velocidad de hibridacion entre dos segmentos polinucleotidicos complementarios en un medio acuoso. Expired ES8609478A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US06/672,941 US4689294A (en) 1984-11-19 1984-11-19 Enhancement of hybridization of nucleic acids by anionic polymers

Publications (2)

Publication Number Publication Date
ES8609478A1 true ES8609478A1 (es) 1986-09-01
ES549043A0 ES549043A0 (es) 1986-09-01

Family

ID=24700657

Family Applications (1)

Application Number Title Priority Date Filing Date
ES549043A Expired ES8609478A1 (es) 1984-11-19 1985-11-19 Un metodo para aumentar la velocidad de hibridacion entre dos segmentos polinucleotidicos complementarios en un medio acuoso.

Country Status (8)

Country Link
US (1) US4689294A (es)
EP (1) EP0184017B1 (es)
JP (1) JPS61128900A (es)
AU (1) AU560781B2 (es)
CA (1) CA1254526A (es)
DE (1) DE3566903D1 (es)
ES (1) ES8609478A1 (es)
IL (1) IL76828A (es)

Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ZA871554B (en) * 1986-03-05 1987-12-30 Molecular Diagnostics Inc Detection of microroganisms in a nucleic acid containing sample
US5034314A (en) * 1986-12-22 1991-07-23 Olin Corporation Method and kit for separating double-stranded nucleic acid from a single-stranded/double-stranded mixture of nucleic acids
AU601021B2 (en) * 1987-03-11 1990-08-30 Molecular Diagnostics, Inc. Assay for necleic acid sequences in a sample
CA1317860C (en) * 1987-04-01 1993-05-18 Daniel Louis Kacian Techniques for preparing specimens for bacterial assays
WO1989004874A1 (en) * 1987-11-03 1989-06-01 Molecular Biosystems, Inc. Method and apparatus for fixation of biopolymers to a solid support by centrifugation
US5116727A (en) * 1989-08-31 1992-05-26 Iniziative Marittime 1991, S.R.L. Hybridization method and composition with alternating polysaccharide of sulfated N-acetylgalactosamine
EP0503000A4 (en) * 1989-12-01 1993-04-14 The Board Of Trustees Of The Leland Stanford Junior University Promotion of high specificity molecular assembly
US5853986A (en) * 1990-04-02 1998-12-29 Becton Dickinson And Company Chemical promotion of nucleic acid hybridization
GB9114180D0 (en) * 1991-07-01 1991-08-21 Amersham Int Plc Enhancement of polynucleotide hybridisation
US6022689A (en) * 1995-04-07 2000-02-08 University Of New Mexico Situ hybridization slide processes
US5750340A (en) * 1995-04-07 1998-05-12 University Of New Mexico In situ hybridization solution and process
US6194146B1 (en) 1997-06-13 2001-02-27 Bioseparations, Inc. Situ and in vitro hybridization method and buffer
US5948617A (en) * 1997-06-13 1999-09-07 Biospeparations, Inc. Methods of in situ hybridization
GB0001450D0 (en) * 2000-01-21 2000-03-08 Genpoint As Cell isolation method
US7439016B1 (en) 2000-06-15 2008-10-21 Digene Corporation Detection of nucleic acids by type-specific hybrid capture method
US7601497B2 (en) 2000-06-15 2009-10-13 Qiagen Gaithersburg, Inc. Detection of nucleic acids by target-specific hybrid capture method
US6656685B2 (en) 2001-01-29 2003-12-02 Ventana Medical Systems, Inc. Hybridization buffers using low molecular weight dextran sulfate and methods for their use
US20040234964A1 (en) * 2003-05-19 2004-11-25 Affymetrix, Inc. Methods for reducing background on polynucleotide arrays
JP2008048067A (ja) 2006-08-11 2008-02-28 Matsushita Electric Works Ltd 補聴器
US8288520B2 (en) 2008-10-27 2012-10-16 Qiagen Gaithersburg, Inc. Fast results hybrid capture assay and system
JP6108661B2 (ja) * 2009-01-28 2017-04-05 キアジェン ゲイサーズバーグ インコーポレイテッド 配列特異的な大量試料調製方法およびアッセイ法
CA2760542A1 (en) * 2009-05-01 2010-11-04 Qiagen Gaithersburg, Inc. A non-target amplification method for detection of rna splice-forms in a sample
EP2478087B1 (en) 2009-09-14 2017-01-18 QIAGEN Gaithersburg, Inc. Compositions and methods for recovery of nucleic acids or proteins from tissue samples fixed in cytology media
BR112012018545A2 (pt) 2010-01-29 2016-05-03 Qiagen Gaithersburg Inc método de determinação e confirmação da presença de um hpv em uma amostra
WO2011094514A1 (en) 2010-01-29 2011-08-04 Qiagen Gaithersburg, Inc. Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids
JP2013528049A (ja) 2010-05-19 2013-07-08 キアゲン ガイサーズバーグ アイエヌシー． 核酸の配列特異的な精製及び多重分析のための方法及び組成物
US9376727B2 (en) 2010-05-25 2016-06-28 Qiagen Gaithersburg, Inc. Fast results hybrid capture assay and associated strategically truncated probes
CA2828224A1 (en) 2011-02-24 2012-08-30 Qiagen Gaithersburg, Inc. Materials and methods for detection of hpv nucleic acids
WO2017041005A1 (en) 2015-09-03 2017-03-09 Abbott Molecular Inc. Hybridization buffers comprising an alkyl diester
KR20170050390A (ko) * 2015-10-30 2017-05-11 (주)아모레퍼시픽 음전하 물질을 포함하는 미세먼지 방진용 조성물
WO2017119930A1 (en) * 2016-01-08 2017-07-13 Abbott Molecular Inc. Hybridization buffers comprising guanidinium thiocyanate

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4302204A (en) * 1979-07-02 1981-11-24 The Board Of Trustees Of Leland Stanford Junior University Transfer and detection of nucleic acids
FR2477033A1 (fr) * 1980-02-28 1981-09-04 Ki Med I Gel polyacrylamidique pour emploi medical et biologique et procede de sa preparation
US4358535A (en) * 1980-12-08 1982-11-09 Board Of Regents Of The University Of Washington Specific DNA probes in diagnostic microbiology

Also Published As

Publication number Publication date
EP0184017A2 (en) 1986-06-11
JPS6231920B2 (es) 1987-07-10
JPS61128900A (ja) 1986-06-16
DE3566903D1 (en) 1989-01-26
CA1254526A (en) 1989-05-23
IL76828A (en) 1989-09-10
AU560781B2 (en) 1987-04-16
AU5000185A (en) 1986-05-29
IL76828A0 (en) 1986-02-28
ES549043A0 (es) 1986-09-01
EP0184017A3 (en) 1987-03-18
EP0184017B1 (en) 1988-12-21
US4689294A (en) 1987-08-25

Similar Documents

Publication Publication Date Title
ES8609478A1 (es) Un metodo para aumentar la velocidad de hibridacion entre dos segmentos polinucleotidicos complementarios en un medio acuoso.
Dorsey et al. The effect of palmityl coenzyme A on pigeon liver fatty acid synthetase
HU9601593D0 (en) Solution phase nucleic acid sandwich assays having reduced background noise
DE19681033D2 (de) Verfahren zur Quantifizierung von Tumorzellen in einer Körperflüssigkeit und dazu geeignete Testkits
ATE50647T1 (de) Verfahren zur quantifizierung von zellpopulationen bzw. subpopulationen sowie hierfuer geeignetes reagenz.
ATE64468T1 (de) Verfahren zur optischen anzeige der direkten oder indirekten bestimmung der reaktion zwischen einem spezifischen bindungsmittel und der korrespondierenden akzeptorsubstanz in ''blot overlay''-testverfahren.
EP0103558A3 (en) Method for quantitative determination of a biospecific affinity reaction
CA2105944A1 (en) Detection of nucleic acids in blood
EP0439222A3 (en) Water-insoluble reagent, nucleic acid probe, test kit and diagnostic and purification methods
ES2010094A6 (es) Multiplicacion y defeccion de secuencias de acido nucleico por sintesis de transcritos de arn especifica.
ES8802257A1 (es) Un metodo para detectar cuantitativamente una secuencia seleccionada de oligo-o-poli-desoxinucleotido en una muestra.
IL75182A0 (en) Nucleic acid hybridization assay employing detactable anti-hybrid antibodies
EP0256932A3 (en) Use of oxidase enzyme systems in chemiluminescent assays
ES485763A1 (es) Un metodo directo para la determinacion de hierro en suero sanguineo
ES8607560A1 (es) Un metodo para detectar una secuencia nucleotidica deteminada en una muestra
EP0128018A3 (en) Molecular genetic probe, and method of forming same, assay technique and kit using said molecular genetic probe
ATE152116T1 (de) Schnellere methode für die reassoziation von nukleinsäure
ES8607557A1 (es) Un metodo para detectar una secuencia polinuscleotidica determinada en un medio a ensayar
FI902934A0 (fi) Foerfarande foer bestaemning av nukleinsyra.
ZA875084B (en) Process and agent for the detection of an analyte
ATE33269T1 (de) Methode zur vorbehandlung einer probe, zusammensetzung hierfuer und diese enthaltender testsatz fuer eine agglutinationsbestimmung.
ATE204580T1 (de) Verfahren zur gewinnung von cephalexin
JO1656B1 (en) New method of analysis
DE69019770T2 (de) Verfahren zur Reinigung, Vervielfachung und zum Nachweis einer Nukleinsäure.
GB1393724A (en) Quantitatively determining cholesterol in a sample