ES8605824A1 - Un procedimiento para la preparacion de un peptido en forma sustancialmente pura. - Google Patents

Un procedimiento para la preparacion de un peptido en forma sustancialmente pura.

Info

Publication number
ES8605824A1
ES8605824A1 ES535125A ES535125A ES8605824A1 ES 8605824 A1 ES8605824 A1 ES 8605824A1 ES 535125 A ES535125 A ES 535125A ES 535125 A ES535125 A ES 535125A ES 8605824 A1 ES8605824 A1 ES 8605824A1
Authority
ES
Spain
Prior art keywords
ser
arg
leu
gly
pro
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES535125A
Other languages
English (en)
Other versions
ES535125A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Institut de Recherches Cliniques de Montreal IRCM
Original Assignee
Institut de Recherches Cliniques de Montreal IRCM
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Institut de Recherches Cliniques de Montreal IRCM filed Critical Institut de Recherches Cliniques de Montreal IRCM
Publication of ES8605824A1 publication Critical patent/ES8605824A1/es
Publication of ES535125A0 publication Critical patent/ES535125A0/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/58—Atrial natriuretic factor complex; Atriopeptin; Atrial natriuretic peptide [ANP]; Cardionatrin; Cardiodilatin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Endocrinology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cardiology (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

PROCEDIMIENTO PARA LA PREPARACION DE UN PEPTIDO EN FORMA SUSTANCIALMENTE PURA. COMPRENDE: A) HOMOGENEIZAR ATRIOS DE RATA EN UNA SOLUCION ACUOSA DE ACIDO ACETICO CON EDTA 1-5 MM, FLUORURO DE FINILMETILSULFONILO 1 MM, PEPSTATINA; B) CENTRIFUGAR, SEPARAR EL LIQUIDO Y CONGELAR; C) DESCONGELAR Y CENTRIFUGAR; D) PASAR EL LIQUIDO POR OCTADECILSILANO; E) DEPOSITAR EL MATERIAL SOBRE PERLAS DE POLIACRILAMIDA; F) ELUIR CON ACIDO ACETICO 0,1 M Y SEPARAR EL MATERIAL EN UNA REGION DE BAJO PESO MOLECULAR, OTRA DE PESO INTERMEDIO Y OTRA DE PESO ELEVADO; G) REUNIR LAS FRACCIONES DE PESO BAJO O INTERMEDIO; H) APLICARLAS A UNA COLUMNA DE PERLAS DE AGAROSE CON GRUPOS CARBOXIMETILO; I) ELUIR CON ACETATO AMONICO 0,01 A 1 M A PH 5; J) REUNIR LAS FRACCIONES Y APLICAR A UN CAMBIADOR DE ION CATIONICO; K) ELUIR CON ACETATO DE TRIETILAMINA 0,02 A 1,0 M A PH 6,5; L) SELECCIONAR LAS FRACCIONES Y PURIFICAR POR HPLC EN FASE INSERVA SOBRE CIANOPROPILSILANO CON TFA AL 0,1% Y ACETONITRILO; M) PURIFICAR LAS FRACCIONES SOBRE OCTADECILSILANO; Y N) PURIFICAR POR APLICACION DE LAS FRACCIONES A OCTADECILSILANO CON ACIDO HEPTAFLUOROBUTIRICO AL 0,13% U ACETONITRILO PARA OBTENER X-CYS-PHE-(GLY)2-ARG-ILE-ASP-ARG-ILE-GLY-ALA-GLN-SER-GLY-LEU-GLY-CYS-Y-Z. SIENDO: H, SER- Y OTROS; Y, -ASN-SER, Y OTROS; Y Z, OH O NH2.
ES535125A 1983-08-29 1984-08-14 Un procedimiento para la preparacion de un peptido en forma sustancialmente pura. Expired ES8605824A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CA435537 1983-08-29
CA449640 1984-03-15

Publications (2)

Publication Number Publication Date
ES8605824A1 true ES8605824A1 (es) 1986-04-01
ES535125A0 ES535125A0 (es) 1986-04-01

Family

ID=25670136

Family Applications (1)

Application Number Title Priority Date Filing Date
ES535125A Expired ES8605824A1 (es) 1983-08-29 1984-08-14 Un procedimiento para la preparacion de un peptido en forma sustancialmente pura.

Country Status (8)

Country Link
US (1) US4607023A (es)
EP (1) EP0140731A3 (es)
AU (1) AU572173B2 (es)
DK (1) DK376684A (es)
ES (1) ES8605824A1 (es)
GR (1) GR80235B (es)
IL (1) IL72572A0 (es)
PT (1) PT79136B (es)

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0116784B1 (en) * 1982-02-22 1992-06-10 Queen's University At Kingston Atrial natriuretic factor
JPS60136596A (ja) * 1983-12-26 1985-07-20 Suntory Ltd ペプチド及びこれを有効成分とする利尿剤
US4618600A (en) * 1984-04-19 1986-10-21 Biotechnology Research Associates, J.V. Novel polypeptide diuretic/vasodilators
US5212286A (en) * 1984-04-19 1993-05-18 Scios Nova Inc. Atrial natriuretic/vasodilator peptide compounds
JPH0672155B2 (ja) * 1984-08-24 1994-09-14 塩野義製薬株式会社 α−ｈＡＮＰ抗血清作製用ポリペプチド
JPH0672157B2 (ja) * 1984-08-29 1994-09-14 味の素株式会社 新規ペプチド
US4670540A (en) * 1984-08-29 1987-06-02 Ajinomoto Co., Inc. Novel peptide
US4757048A (en) * 1985-11-05 1988-07-12 Biotechnology Research Associates J.V. Synthetic analogs of atrial natriuretic peptides
IE862891L (en) * 1985-11-05 1987-05-05 Behringwerke Ag Analogs of atrial natriuretic peptides
DE3614833A1 (de) * 1986-01-16 1987-07-23 Hoechst Ag Peptide mit vasorelaxierender, natriuretischer und diuretischer wirkung, verfahren zu ihrer herstellung, diese enthaltende mittel und ihre verwendung
EP0232078A3 (en) * 1986-01-31 1990-03-14 Merck & Co. Inc. Peptides having anf activity
US4721704A (en) * 1986-05-09 1988-01-26 Peninsula Laboratories, Inc. Potent synthetic atrial peptide analogs
EP0246795A3 (en) * 1986-05-20 1990-04-04 Advanced Peptide Development Limited Synthetic natriuretic peptides
DE3619633A1 (de) * 1986-06-11 1987-12-17 Hoechst Ag Peptide mit einfluss auf die diurese und natriurese, verfahren zu ihrer herstellung, diese enthaltende mittel und ihre verwendung
US5159061A (en) * 1986-09-29 1992-10-27 Takeda Chemical Industries, Ltd. Atrial natriuretic peptide derivative
US4804650A (en) * 1986-10-28 1989-02-14 Biotechnology Research Associates, J.V. Analogs of atrial natriuretic peptides
US4816443A (en) * 1987-05-19 1989-03-28 Merck & Co., Inc. Piptides having ANF activity
US4987121A (en) * 1988-05-27 1991-01-22 Center For Innovative Technology Erythropoietic factor
US5114923A (en) * 1988-05-31 1992-05-19 California Biotechnology Inc. Recombinant techniques for production of novel natriuretic and vasodilator peptides
CA1339210C (en) * 1988-05-31 1997-08-05 John Lewicki Recombinant techniques for production of novel natriuretic and vasodilator peptides
US5043321A (en) * 1988-07-11 1991-08-27 Center For Innovative Technology Adrenocorticotropin release inhibiting factor
GB9125024D0 (en) * 1991-11-25 1992-01-22 Kirby Julian Rheumatoid arthritus treatment
JPH10500969A (ja) * 1994-06-02 1998-01-27 ベーリンガー マンハイム ゲゼルシャフト ミット ベシュレンクテル ハフツング カルジオジラチンフラグメントの調製方法、高度に精製されたカルジオジラチンフラグメント及びそれらの調製のための中間生成物類
AU2003214038A1 (en) * 2002-04-03 2003-10-13 Chr. Hansen A/S Peptides with anti-hypertensive properties
US7718171B2 (en) * 2003-04-07 2010-05-18 Chr. Hansen A/S Reducing heart rate in mammals using milk derived fermentation products produced using Lactobacillus helveticus
US7291501B2 (en) 2003-07-16 2007-11-06 Abbott Laboratories Stable compositions for measuring human natriuretic peptides
US7445933B2 (en) * 2003-07-16 2008-11-04 Abbott Laboratories, Inc. Stable calibrators or controls for measuring human natriuretic peptides
US7731965B2 (en) * 2005-02-17 2010-06-08 Abbott Lab Human ring specific BNP antibodies
US7939069B2 (en) 2005-11-09 2011-05-10 Abbott Laboratories Human BNP immunospecific antibodies
CA2629438C (en) * 2005-11-09 2014-02-25 Abbott Laboratories Human bnp immunospecific antibodies
US20080064045A1 (en) * 2006-09-07 2008-03-13 Huaiqin Wu Biomarker fragments for the detection of human BNP
WO2024086680A2 (en) 2022-10-21 2024-04-25 Eli Lilly And Company Long-acting natriuretic peptides and uses thereof

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0116784B1 (en) * 1982-02-22 1992-06-10 Queen's University At Kingston Atrial natriuretic factor
US4496544A (en) * 1983-11-10 1985-01-29 Washington University Atrial Peptides
JPS60136596A (ja) * 1983-12-26 1985-07-20 Suntory Ltd ペプチド及びこれを有効成分とする利尿剤
US4508712A (en) * 1984-01-10 1985-04-02 Washington University Atrial peptide
US4952561A (en) * 1984-02-07 1990-08-28 Merck & Co., Inc. Cardiac atrial peptides

Also Published As

Publication number Publication date
PT79136A (en) 1984-09-01
IL72572A0 (en) 1984-11-30
AU572173B2 (en) 1988-05-05
EP0140731A3 (en) 1987-02-04
GR80235B (en) 1985-01-02
EP0140731A2 (en) 1985-05-08
AU3123284A (en) 1985-03-07
DK376684D0 (da) 1984-08-03
ES535125A0 (es) 1986-04-01
US4607023A (en) 1986-08-19
PT79136B (en) 1986-11-13
DK376684A (da) 1985-03-01

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