ES8605579A1 - Procedimiento para preparar proteinas encarioticas, procarioticas o viricas. - Google Patents

Procedimiento para preparar proteinas encarioticas, procarioticas o viricas.

Info

Publication number
ES8605579A1
ES8605579A1 ES547600A ES547600A ES8605579A1 ES 8605579 A1 ES8605579 A1 ES 8605579A1 ES 547600 A ES547600 A ES 547600A ES 547600 A ES547600 A ES 547600A ES 8605579 A1 ES8605579 A1 ES 8605579A1
Authority
ES
Spain
Prior art keywords
signal sequence
proteins
transportation
expression systems
synthetic signal
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES547600A
Other languages
English (en)
Other versions
ES547600A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hoechst AG
Original Assignee
Hoechst AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoechst AG filed Critical Hoechst AG
Publication of ES8605579A1 publication Critical patent/ES8605579A1/es
Publication of ES547600A0 publication Critical patent/ES547600A0/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Endocrinology (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Diabetes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Saccharide Compounds (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

PROCEDIMIENTO PARA LA OBTENCION DE PROTEINAS EUCARIOTICAS, PROCARIOTICAS Y VIRICAS. CONSISTE EN LA COPULACION DEL GEN PARA LA PROTEINA DESEADA A UNA SECUENCIA DE SEÑAL SINTETICA QUE CORRESPONDE EN ESENCIA A UNA SECUENCIA DE SEÑAL NATURAL, PERO QUE CONTIENE UNO O VARIOS LUGARES DE CORTE PARA ENDONUCLEASAS, NO CONTENIDOS EN EL ADN NATURAL; INCORPORACION DEL GEN DE FUSION ASI OBTENIDO EN UN VECTOR, CON LO QUE SE TRANSFORMA UNA CELULA HOSPEDANTE PROCARIOTICA O EUCARIOTICA; LLEVAR A EXPRESION LA PROTEINA EN LA CELULA HOSPEDANTE CITADA; Y, FINALMENTE, TRANSPORTE DE LA PROTEINA EXPRESADA FUERA DEL CITOPLASMA. TIENE APLICACIONES PARA LA OBTENCION DE PEPTIDOS CON INTERES FARMACOLOGICO, COMO HORMONAS, LINFOQUINAS, INTERFERONES, FACTORES DE COAGULACION DE LA SANGRE Y VACUNAS.
ES547600A 1984-10-06 1985-10-04 Procedimiento para preparar proteinas encarioticas, procarioticas o viricas. Expired ES8605579A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19843436818 DE3436818A1 (de) 1984-10-06 1984-10-06 Synthetische signalsequenz zum transport von proteinen in expressionssystemen

Publications (2)

Publication Number Publication Date
ES8605579A1 true ES8605579A1 (es) 1986-03-16
ES547600A0 ES547600A0 (es) 1986-03-16

Family

ID=6247347

Family Applications (1)

Application Number Title Priority Date Filing Date
ES547600A Expired ES8605579A1 (es) 1984-10-06 1985-10-04 Procedimiento para preparar proteinas encarioticas, procarioticas o viricas.

Country Status (15)

Country Link
EP (1) EP0177827B1 (es)
JP (1) JPS6188883A (es)
AT (1) ATE97445T1 (es)
AU (1) AU595486B2 (es)
CA (1) CA1340280C (es)
DE (2) DE3436818A1 (es)
DK (1) DK175511B1 (es)
ES (1) ES8605579A1 (es)
GR (1) GR852405B (es)
HU (1) HU197355B (es)
IE (1) IE63262B1 (es)
IL (1) IL76573A (es)
NZ (1) NZ213717A (es)
PH (1) PH30819A (es)
PT (1) PT81253B (es)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0196864A3 (en) * 1985-03-25 1988-03-23 Cetus Corporation Alkaline phosphatase-mediated processing and secretion of recombinant proteins, dna sequences for use therein and cells transformed using such sequences
IT1196484B (it) * 1986-07-11 1988-11-16 Sclavo Spa Vettore ad espressione e secrezione in lieviti,utile per la preparazione di proteine eterologhe
US5426036A (en) * 1987-05-05 1995-06-20 Hoechst Aktiengesellschaft Processes for the preparation of foreign proteins in streptomycetes
DE68917759T2 (de) * 1988-03-18 1995-04-27 Wang Laboratories Verteilte Referenz- und Änderungstabelle für ein virtuelles Speichersystem.
EP1613158A4 (en) 2003-03-27 2007-08-22 Ptc Therapeutics Inc METHOD FOR IDENTIFYING COMPOUNDS WITH A TRNA-SPLICEN ENDONUCLEASE AT THE PLACE OF ATTACK, AND USES THEREOF FOR PROVIDING THESE COMPOUNDS AS PROLIFERATION-INHIBITABLE AGENTS
CA2520510A1 (en) 2003-03-27 2004-10-14 Ptc Therapeutics, Inc. Methods of identifying compounds that target trna splicing endonuclease and uses of said compounds as anti-fungal agents
DE04777457T1 (de) 2003-07-02 2008-08-14 Ptc Therapeutics, Inc. Rna prozessierende proteinkomplexe und verwendungen davon

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5939899A (ja) * 1982-08-27 1984-03-05 Gakuzo Tamura 新規ベクタ−
US4711844A (en) * 1983-03-09 1987-12-08 Cetus Corporation Modified signal peptides
NZ207925A (en) * 1983-04-25 1988-05-30 Genentech Inc Yeast expression vehicle consisting of a yeast promoter and signal peptide encoding region linked to a heterologus peptide coding region; expression and culture
AU3011684A (en) * 1983-05-19 1984-12-04 Public Health Research Institute Of The City Of New York, Inc., The Expression and secretion vectors & method of constructing vectors
US4663280A (en) * 1983-05-19 1987-05-05 Public Health Research Institute Of The City Of New York Expression and secretion vectors and method of constructing vectors
JPS6030687A (ja) * 1983-08-01 1985-02-16 Wakunaga Seiyaku Kk Ｄνａ遺伝子，その製造法およびそれを含むプラスミド
EP0170266B1 (en) * 1984-07-30 1993-03-24 Wakunaga Seiyaku Kabushiki Kaisha Process for producing protein, and vector, recombinant dna and transformant used therefor

Also Published As

Publication number Publication date
PT81253B (pt) 1987-11-30
JPS6188883A (ja) 1986-05-07
PH30819A (en) 1997-10-17
IL76573A (en) 1992-06-21
NZ213717A (en) 1989-01-06
IE63262B1 (en) 1995-04-05
DK453285A (da) 1986-04-07
ATE97445T1 (de) 1993-12-15
GR852405B (es) 1986-02-04
DK175511B1 (da) 2004-11-15
ES547600A0 (es) 1986-03-16
DE3436818A1 (de) 1986-04-10
HUT40164A (en) 1986-11-28
IL76573A0 (en) 1986-02-28
EP0177827A2 (de) 1986-04-16
IE852440L (en) 1986-04-06
AU4833385A (en) 1986-04-10
DK453285D0 (da) 1985-10-04
CA1340280C (en) 1998-12-22
AU595486B2 (en) 1990-04-05
DE3587660D1 (de) 1993-12-23
EP0177827B1 (de) 1993-11-18
PT81253A (de) 1985-11-01
EP0177827A3 (en) 1987-12-02
HU197355B (en) 1989-03-28

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Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 20051219