IL76573A - Synthetic nucleotide sequence coding for a signal sequence for the transport of proteins in expression systems that have a cleavage site for an endonuclease not contained in the natural dna - Google Patents

Synthetic nucleotide sequence coding for a signal sequence for the transport of proteins in expression systems that have a cleavage site for an endonuclease not contained in the natural dna

Info

Publication number
IL76573A
IL76573A IL76573A IL7657385A IL76573A IL 76573 A IL76573 A IL 76573A IL 76573 A IL76573 A IL 76573A IL 7657385 A IL7657385 A IL 7657385A IL 76573 A IL76573 A IL 76573A
Authority
IL
Israel
Prior art keywords
transport
endonuclease
proteins
contained
cleavage site
Prior art date
Application number
IL76573A
Other versions
IL76573A0 (en
Original Assignee
Hoechst Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoechst Ag filed Critical Hoechst Ag
Publication of IL76573A0 publication Critical patent/IL76573A0/en
Publication of IL76573A publication Critical patent/IL76573A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Endocrinology (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Diabetes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The DNA of a natural signal sequence is modified by incorporation of cleavage sites for endonucleases and is thus made suitable for incorporation in any desired vectors by the building block principle. The vectors modified in this way then bring about transport of the encoded protein out of the cytoplasm. <IMAGE>
IL76573A 1984-10-06 1985-10-04 Synthetic nucleotide sequence coding for a signal sequence for the transport of proteins in expression systems that have a cleavage site for an endonuclease not contained in the natural dna IL76573A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19843436818 DE3436818A1 (en) 1984-10-06 1984-10-06 SYNTHETIC SIGNAL SEQUENCE FOR TRANSPORTING PROTEINS IN EXPRESSION SYSTEMS

Publications (2)

Publication Number Publication Date
IL76573A0 IL76573A0 (en) 1986-02-28
IL76573A true IL76573A (en) 1992-06-21

Family

ID=6247347

Family Applications (1)

Application Number Title Priority Date Filing Date
IL76573A IL76573A (en) 1984-10-06 1985-10-04 Synthetic nucleotide sequence coding for a signal sequence for the transport of proteins in expression systems that have a cleavage site for an endonuclease not contained in the natural dna

Country Status (15)

Country Link
EP (1) EP0177827B1 (en)
JP (1) JPS6188883A (en)
AT (1) ATE97445T1 (en)
AU (1) AU595486B2 (en)
CA (1) CA1340280C (en)
DE (2) DE3436818A1 (en)
DK (1) DK175511B1 (en)
ES (1) ES8605579A1 (en)
GR (1) GR852405B (en)
HU (1) HU197355B (en)
IE (1) IE63262B1 (en)
IL (1) IL76573A (en)
NZ (1) NZ213717A (en)
PH (1) PH30819A (en)
PT (1) PT81253B (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0196864A3 (en) * 1985-03-25 1988-03-23 Cetus Corporation Alkaline phosphatase-mediated processing and secretion of recombinant proteins, dna sequences for use therein and cells transformed using such sequences
IT1196484B (en) * 1986-07-11 1988-11-16 Sclavo Spa YEAST EXPRESSION AND SECRETION VECTOR, USEFUL FOR THE PREPARATION OF HETEROLOGICAL PROTEINS
US5426036A (en) * 1987-05-05 1995-06-20 Hoechst Aktiengesellschaft Processes for the preparation of foreign proteins in streptomycetes
EP0333215B1 (en) * 1988-03-18 1994-08-31 Wang Laboratories Inc. Distributed reference and change table for a virtual memory system
JP4634371B2 (en) 2003-03-27 2011-02-16 ピーティーシー セラピューティクス，インコーポレーテッド Methods for identifying compounds that target tRNA splicing endonucleases and their use as antiproliferative agents
CA2520510A1 (en) 2003-03-27 2004-10-14 Ptc Therapeutics, Inc. Methods of identifying compounds that target trna splicing endonuclease and uses of said compounds as anti-fungal agents
DE04777457T1 (en) 2003-07-02 2008-08-14 Ptc Therapeutics, Inc. RNA PROCESSING PROTEIN COMPLEXES AND USES THEREOF

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5939899A (en) * 1982-08-27 1984-03-05 Gakuzo Tamura Novel vector
US4711844A (en) * 1983-03-09 1987-12-08 Cetus Corporation Modified signal peptides
NZ207925A (en) * 1983-04-25 1988-05-30 Genentech Inc Yeast expression vehicle consisting of a yeast promoter and signal peptide encoding region linked to a heterologus peptide coding region; expression and culture
US4663280A (en) * 1983-05-19 1987-05-05 Public Health Research Institute Of The City Of New York Expression and secretion vectors and method of constructing vectors
AU3011684A (en) * 1983-05-19 1984-12-04 Public Health Research Institute Of The City Of New York, Inc., The Expression and secretion vectors & method of constructing vectors
JPS6030687A (en) * 1983-08-01 1985-02-16 Wakunaga Seiyaku Kk Dna gene, its preparation and plasmid containing the same
EP0170266B1 (en) * 1984-07-30 1993-03-24 Wakunaga Seiyaku Kabushiki Kaisha Process for producing protein, and vector, recombinant dna and transformant used therefor

Also Published As

Publication number Publication date
DE3587660D1 (en) 1993-12-23
IE63262B1 (en) 1995-04-05
EP0177827A2 (en) 1986-04-16
JPS6188883A (en) 1986-05-07
AU595486B2 (en) 1990-04-05
IE852440L (en) 1986-04-06
PT81253A (en) 1985-11-01
HUT40164A (en) 1986-11-28
ES547600A0 (en) 1986-03-16
DE3436818A1 (en) 1986-04-10
IL76573A0 (en) 1986-02-28
PH30819A (en) 1997-10-17
NZ213717A (en) 1989-01-06
DK453285A (en) 1986-04-07
CA1340280C (en) 1998-12-22
DK175511B1 (en) 2004-11-15
HU197355B (en) 1989-03-28
EP0177827A3 (en) 1987-12-02
ES8605579A1 (en) 1986-03-16
AU4833385A (en) 1986-04-10
DK453285D0 (en) 1985-10-04
ATE97445T1 (en) 1993-12-15
GR852405B (en) 1986-02-04
PT81253B (en) 1987-11-30
EP0177827B1 (en) 1993-11-18

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