ES8600403A1 - Un procedimiento para producir un peptido que comprende un peptido de exo-senal unido operativamente a una proteina deseada - Google Patents

Un procedimiento para producir un peptido que comprende un peptido de exo-senal unido operativamente a una proteina deseada

Info

Publication number
ES8600403A1
ES8600403A1 ES537330A ES537330A ES8600403A1 ES 8600403 A1 ES8600403 A1 ES 8600403A1 ES 537330 A ES537330 A ES 537330A ES 537330 A ES537330 A ES 537330A ES 8600403 A1 ES8600403 A1 ES 8600403A1
Authority
ES
Spain
Prior art keywords
signal peptides
dna sequences
exo
signal
sequence encoding
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES537330A
Other languages
English (en)
Other versions
ES537330A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novartis Vaccines and Diagnostics Inc
Original Assignee
Cetus Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cetus Corp filed Critical Cetus Corp
Publication of ES8600403A1 publication Critical patent/ES8600403A1/es
Publication of ES537330A0 publication Critical patent/ES537330A0/es
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/54—Interleukins [IL]
    • C07K14/55—IL-2
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/034—Fusion polypeptide containing a localisation/targetting motif containing a motif for targeting to the periplasmic space of Gram negative bacteria as a soluble protein, i.e. signal sequence should be cleaved
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S930/00—Peptide or protein sequence
    • Y10S930/01—Peptide or protein sequence
    • Y10S930/30—Signal or leader sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Endocrinology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

PROCEDIMIENTO PARA PRODUCIR UN PEPTIDO QUE COMPRENDE UN PEPTIDO DE EXO-SEN/AL UNIDO OPERATIVAMENTE A UNA PROTEINA DESEADA.COMPRENDE: A) PROPORCIONAR UNA SECUENCIA DE ADN QUE CODIFICA UN PEPTIDO DE EXO-SEN/AL DE TIPO SILVESTRE QUE TIENE UNA REGION FACILITADORA CON UN RESTO DE CISTEINA, PARA FORMAR LIPOPROTEINA LIGADA A LA MEMBRANA; B) ALTERAR A LA SECUENCIA DE ADN, PARA OBTENER QUE UN PEPTIDO DE EXO-SEN/AL CODIFICADO POR LA SECUENCIA DE ADN ALTERADA QUE CONTIENE UN AMINOACIDO X DISTINTO DE LA CISTEINA EN LA REGION FACILITADORA, NO FORME LIPOPROTEINA LIGADA A LA MEMBRANA; C) UNIR LA SECUENCIA DE ADN DE (A) A LA SECUENCIA DE ADN ALTERADA QUE CODIFICA UN PEPTIDO DE EXO-SEN/AL ALTERADO PARA CONSERVAR AL AMINOACIDO X Y A LA SECUENCIA DE ADN DE (A); D) TRANSFORMAR UNA CELULA PROCARIOTICA CON UN VECTOR REPLICABLE QUE CONTIENE LAS SECUENCIAS DE ADN UNIDAS DE LAS OPERACIONES (A), (B) Y (C); Y E) DESARROLLAR A LA CELULA PROCARIOTICA TRANSFORMADA EN UN MEDIO NUTRIENTE ADECUADO, PARA OBTENER UN PEPTIDO.
ES537330A 1983-03-09 1984-11-02 Un procedimiento para producir un peptido que comprende un peptido de exo-senal unido operativamente a una proteina deseada Granted ES537330A0 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US06/473,820 US4711844A (en) 1983-03-09 1983-03-09 Modified signal peptides

Publications (2)

Publication Number Publication Date
ES8600403A1 true ES8600403A1 (es) 1985-09-16
ES537330A0 ES537330A0 (es) 1985-09-16

Family

ID=23881126

Family Applications (2)

Application Number Title Priority Date Filing Date
ES537329A Granted ES537329A0 (es) 1983-03-09 1984-11-02 Un procedimiento para producir una secuencia de adn que codifica un peptido de exo-senal
ES537330A Granted ES537330A0 (es) 1983-03-09 1984-11-02 Un procedimiento para producir un peptido que comprende un peptido de exo-senal unido operativamente a una proteina deseada

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ES537329A Granted ES537329A0 (es) 1983-03-09 1984-11-02 Un procedimiento para producir una secuencia de adn que codifica un peptido de exo-senal

Country Status (9)

Country Link
US (1) US4711844A (es)
EP (1) EP0138948B1 (es)
JP (1) JPS60500701A (es)
DE (1) DE3476557D1 (es)
DK (1) DK534584D0 (es)
ES (2) ES537329A0 (es)
FI (1) FI844375L (es)
IT (1) IT1199087B (es)
WO (1) WO1984003519A1 (es)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5411873A (en) * 1984-05-29 1995-05-02 Genencor, Inc. Process for producing heterologous polypeptides
US5840522A (en) * 1984-09-20 1998-11-24 Chiron Corporation Recombinant lectins
DE3586386T2 (de) * 1984-10-05 1993-01-14 Genentech Inc Dna, zellkulturen und verfahren zur sekretion von heterologen proteinen und periplasmische proteinrueckgewinnung.
US4963495A (en) * 1984-10-05 1990-10-16 Genentech, Inc. Secretion of heterologous proteins
DE3436818A1 (de) * 1984-10-06 1986-04-10 Hoechst Ag, 6230 Frankfurt Synthetische signalsequenz zum transport von proteinen in expressionssystemen
IT1177378B (it) * 1984-12-11 1987-08-26 Anic Spa Vettore plasmidico psm112 ad espressione in bacillus subtilis
US6084073A (en) * 1985-03-25 2000-07-04 Chiron Corporation Recombinant ricin toxin
JPH0669377B2 (ja) * 1985-11-25 1994-09-07 工業技術院長 Ｄｎａ塩基配列およびベクタ−
EP0237676B1 (en) * 1986-03-07 1998-08-12 Chiron Corporation Recombinant ricin toxin
DK175535B1 (da) * 1987-03-04 2004-11-29 Daiichi Suntory Pharma Co Ltd Fremgangsmåde til fremstilling af et fysiologisk aktivt cysteinholdigt peptid
CA1339757C (en) 1987-04-16 1998-03-17 Robert F. Halenbeck Production of purified biologically active, bacterially produced recombinant human csf-1
US4929700A (en) * 1987-04-16 1990-05-29 Cetus Corporation Production of purified, biologically active, bacterially produced recombinant human CSF-1
WO1988009821A1 (en) * 1987-06-12 1988-12-15 Massachusetts Institute Of Technology Coryneform expression and secretion system
US5171673A (en) * 1988-07-18 1992-12-15 Biotechnica International, Inc. Expression of heterologous dna using the bacillus coagulans amylase gene
US5037743A (en) * 1988-08-05 1991-08-06 Zymogenetics, Inc. BAR1 secretion signal
US5284768A (en) * 1988-08-24 1994-02-08 Sanofi Signal peptide, DNA sequences coding for the latter, expression vectors carrying one of these sequences, gram-negative bacteria transformed by these vectors, and process for the periplasmic production of a polypeptide
US5028593A (en) * 1989-08-15 1991-07-02 Board Of Regents, The University Of Texas System Tuftsin analogs
AU681572B2 (en) * 1991-10-21 1997-09-04 Med Immune, Inc. Bacterial expression vectors containing DNA encoding secretion signals of lipoproteins
US5705362A (en) * 1992-05-25 1998-01-06 Gist-Brocades, N.V. Modified signal sequences
FR2708622B1 (fr) * 1993-08-02 1997-04-18 Raymond Hamers Vecteur recombinant contenant une séquence d'un gène de lipoprotéine de structure pour l'expression de séquences de nucléotides.
AU722630B2 (en) * 1997-02-14 2000-08-10 Higeta Shoyu Co., Ltd. Modified Treponema pallidum-derived antigen protein
US6017694A (en) * 1997-12-19 2000-01-25 American Cyanamid Company Methods of screening for modulators of respiratory syncytial virus matrix protein interaction

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4411994A (en) * 1978-06-08 1983-10-25 The President And Fellows Of Harvard College Protein synthesis
US4351901A (en) * 1980-03-24 1982-09-28 Cetus Corporation Method for single nucleotide alteration
US4338397A (en) * 1980-04-11 1982-07-06 President And Fellows Of Harvard College Mature protein synthesis
GB8308483D0 (en) * 1983-03-28 1983-05-05 Health Lab Service Board Secretion of gene products

Also Published As

Publication number Publication date
DK534584A (da) 1984-11-09
IT8447831A1 (it) 1985-09-09
ES8507613A1 (es) 1985-09-01
FI844375A0 (fi) 1984-11-07
WO1984003519A1 (en) 1984-09-13
US4711844A (en) 1987-12-08
ES537330A0 (es) 1985-09-16
JPS60500701A (ja) 1985-05-16
EP0138948B1 (en) 1989-02-01
DE3476557D1 (en) 1989-03-09
IT8447831A0 (it) 1984-03-09
IT1199087B (it) 1988-12-30
EP0138948A1 (en) 1985-05-02
FI844375L (fi) 1984-11-07
ES537329A0 (es) 1985-09-01
DK534584D0 (da) 1984-11-09

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