ES8504249A1 - Un procedimiento para la expresion de un gen extrano. - Google Patents

Un procedimiento para la expresion de un gen extrano.

Info

Publication number
ES8504249A1
ES8504249A1 ES526324A ES526324A ES8504249A1 ES 8504249 A1 ES8504249 A1 ES 8504249A1 ES 526324 A ES526324 A ES 526324A ES 526324 A ES526324 A ES 526324A ES 8504249 A1 ES8504249 A1 ES 8504249A1
Authority
ES
Spain
Prior art keywords
rbs
site
translation
vector
gtg
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES526324A
Other languages
English (en)
Other versions
ES526324A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Japanese Foundation for Cancer Research
KH Neochem Co Ltd
Original Assignee
Japanese Foundation for Cancer Research
Kyowa Hakko Kogyo Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from JP57177192A external-priority patent/JPS5967297A/ja
Priority claimed from JP58012282A external-priority patent/JPS59140883A/ja
Application filed by Japanese Foundation for Cancer Research, Kyowa Hakko Kogyo Co Ltd filed Critical Japanese Foundation for Cancer Research
Publication of ES8504249A1 publication Critical patent/ES8504249A1/es
Publication of ES526324A0 publication Critical patent/ES526324A0/es
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/565—IFN-beta
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Structure Of Belt Conveyors (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

PROCEDIMIENTO PARA LA EXPRESION DE UN GEN EXTRAN/O.COMPRENDE LAS SIGUIENTES OPERACIONES: PRIMERA, SE ROMPE CON UN ENZIMA DE RESTRICCION UN VECTOR DE EXPRESION QUE CONTIENE UN PROMOTOR, UN SITIO DE UNION DE UN RIBOSOMA Y UN CODON DE INICIACION; SEGUNDA, SE INCORPORA EL GEN EXTRAN/O AL EXTREMO ROMO O SE INCORPORA EL GEN EXTREMO CON UN BLOQUE HOMOPOLIMERICO COMPLEMENTARIO AL BLOQUE HOMOPOLIMERICO ANTERIOR PARA FORMAR UN RECOMBINANTE; Y POR ULTIMO, SE TRANSFORMA UN MICROORGANISMO HUESPED CON EL RECOMBINANTE Y SE CULTIVA EL TRANSFORMANTE ASI OBTENIDO.
ES526324A 1982-10-08 1983-10-07 Un procedimiento para la expresion de un gen extrano. Granted ES526324A0 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP57177192A JPS5967297A (ja) 1982-10-08 1982-10-08 新規ベクタ−
JP58012282A JPS59140883A (ja) 1983-01-28 1983-01-28 新規ベクタ−

Publications (2)

Publication Number Publication Date
ES8504249A1 true ES8504249A1 (es) 1985-04-01
ES526324A0 ES526324A0 (es) 1985-04-01

Family

ID=26347858

Family Applications (1)

Application Number Title Priority Date Filing Date
ES526324A Granted ES526324A0 (es) 1982-10-08 1983-10-07 Un procedimiento para la expresion de un gen extrano.

Country Status (8)

Country Link
EP (1) EP0121569B1 (es)
AU (1) AU575301B2 (es)
DE (1) DE3336586A1 (es)
DK (1) DK280084A (es)
ES (1) ES526324A0 (es)
IL (1) IL69926A (es)
IT (1) IT1160124B (es)
NO (1) NO842296L (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4935370A (en) * 1983-12-23 1990-06-19 Pfizer Inc. Expression plasmids for improved production of heterologous protein in bacteria
DE3484926D1 (de) * 1983-12-23 1991-09-19 Pfizer Expressionsplasmide fuer die herstellung eines heterologen proteins in bakterien.
DE3430683A1 (de) * 1984-08-21 1986-03-06 Hoechst Ag, 6230 Frankfurt Synthetische regulationsregion
DE3650065T2 (de) * 1985-07-31 1995-03-02 Univ Leland Stanford Junior Invasive Mikroorganismen.
US5817478A (en) * 1993-10-05 1998-10-06 Asahi Glass Company Ltd. Multicloning vector, expression vector and production of foreign proteins by using the expression vector

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0787785B2 (ja) * 1980-06-06 1995-09-27 バイオゲン インコーポレイテッド 改良ベクタおよびこの種ベクタの製造方法ならびにクローン化遺伝子の発現方法
GR81946B (es) * 1980-09-25 1984-12-12 Genentech Inc
DK489481A (da) * 1980-11-10 1982-05-11 Searle & Co Plasmidvektor og frmgangsmaade til fremstilling deraf
IL66263A (en) * 1981-08-10 1985-12-31 Genex Corp Expression vectors for introducing a gene into a procaryotic organism
AU561343B2 (en) * 1981-10-19 1987-05-07 Genentech Inc. Human immune interferon by recombinant dna
JPS58110600A (ja) * 1981-12-25 1983-07-01 Kyowa Hakko Kogyo Co Ltd ヒトβ型インタ−フエロン遺伝子を含む組みかえ体プラスミド
US4738927A (en) * 1982-03-31 1988-04-19 Ajinomoto Co. Inc. Gene coded for interleukin-2 polypeptide, recombinant DNA carrying the said gene, a living cell line possessing the recombinant DNA, and method for producing interleukin-2 using the said cell
CA1341562C (en) * 1982-03-31 2007-11-27 Tadatsugu Taniguchi Gene coded for interleukin-2 polypeptide, recombinant dna carrying the said gene, a living cell line possessing the recombinant dna, and method for producing interleukin-2 using the said cell
AU571460B2 (en) * 1982-09-27 1988-04-21 Cancer Institute Of Japanese Foundation For Cancer Research Alpha interferon dna, recombinant plasmid, engineered organism and alpha interferon polypeptide

Also Published As

Publication number Publication date
DK280084D0 (da) 1984-06-07
IT8368033A0 (it) 1983-10-07
IL69926A (en) 1990-02-09
AU575301B2 (en) 1988-07-28
AU1942183A (en) 1984-05-04
EP0121569A4 (en) 1986-04-15
DK280084A (da) 1984-06-07
EP0121569B1 (en) 1990-08-22
EP0121569A1 (en) 1984-10-17
IL69926A0 (en) 1984-01-31
NO842296L (no) 1984-06-07
DE3336586A1 (de) 1984-04-12
ES526324A0 (es) 1985-04-01
IT1160124B (it) 1987-03-04

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Legal Events

Date Code Title Description
MM4A Patent lapsed

Effective date: 19960401