ES8107301A1 - Metodo para aumentar la produccion de proteina por dna - Google Patents

Metodo para aumentar la produccion de proteina por dna

Info

Publication number
ES8107301A1
ES8107301A1 ES487236A ES487236A ES8107301A1 ES 8107301 A1 ES8107301 A1 ES 8107301A1 ES 487236 A ES487236 A ES 487236A ES 487236 A ES487236 A ES 487236A ES 8107301 A1 ES8107301 A1 ES 8107301A1
Authority
ES
Spain
Prior art keywords
dna
plasmids
copy number
high copy
elements
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES487236A
Other languages
English (en)
Other versions
ES487236A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novartis Vaccines and Diagnostics Inc
Original Assignee
Cetus Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cetus Corp filed Critical Cetus Corp
Publication of ES487236A0 publication Critical patent/ES487236A0/es
Publication of ES8107301A1 publication Critical patent/ES8107301A1/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/69—Increasing the copy number of the vector

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

PROCEDIMIENTO PARA AUMENTAR LA PRODUCCION DE PROTEINA POR DNA. CONSISTE EN ELEGIR MUTANTES DEL PLASMIDIO QUE TIENEN GENES REPRESORES ALTERADOS, QUE CONDUCEN A REPETICION DE GRAN NUMERO DE COPIA, E INCAPACITAR A LOS ELEMENTOS TRASPONIBLES DEL PLASMIDIO. LA INCAPATIZACION TIENE LUGAR ELIMINANDO, AL MENOS, PARTE DE DICHOS ELEMENTOS, POR LIGADURA DE LOS EXTREMOS ROMOS DE FRAGMENTOS DE DNA PRODUCIDOS POR DIGESTIONES CON ENZIMAS DE RESTRICCION, Y DONDE LOS FRAGMENTOS SE HACEN DE EXTREMOS ROMOS POR USO DE LA ENZIMA DNA POLIMERASA I; LAS COLAS 3'' DE HEBRA UNICA SE ELIMINAN, Y LAS COLAS 5'' DE HEBRA UNICA SE VUELVEN A EMPAREJAR EN FORMA DE DOBLE HEBRA. LOS MUTANTES ELEGIDOS SON SENSIBLES A LA TEMPERATURA.
ES487236A 1978-12-26 1979-12-24 Metodo para aumentar la produccion de proteina por dna Expired ES8107301A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US97270578A 1978-12-26 1978-12-26

Publications (2)

Publication Number Publication Date
ES487236A0 ES487236A0 (es) 1980-12-16
ES8107301A1 true ES8107301A1 (es) 1980-12-16

Family

ID=25520026

Family Applications (1)

Application Number Title Priority Date Filing Date
ES487236A Expired ES8107301A1 (es) 1978-12-26 1979-12-24 Metodo para aumentar la produccion de proteina por dna

Country Status (9)

Country Link
EP (1) EP0013830B1 (es)
JP (1) JPS55104888A (es)
AT (1) ATE2340T1 (es)
AU (1) AU531722B2 (es)
DE (1) DE2964664D1 (es)
DK (1) DK541979A (es)
ES (1) ES8107301A1 (es)
FI (1) FI793884A (es)
NO (1) NO794133L (es)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4966840A (en) * 1978-12-26 1990-10-30 Cetus Corporation Stable high copy number plasmids
ZA811368B (en) 1980-03-24 1982-04-28 Genentech Inc Bacterial polypedtide expression employing tryptophan promoter-operator
CA1198067A (en) * 1981-02-27 1985-12-17 David H. Gelfand Stable high copy number plasmids
CA1209501A (en) * 1982-09-16 1986-08-12 Nikos Panayotatos Expression vector
JPH0753111B2 (ja) * 1982-09-16 1995-06-07 ベンツォン ファーマ エイ／エス 条件非制御の複製挙動を持つプラスミド
US4710473A (en) * 1983-08-10 1987-12-01 Amgen, Inc. DNA plasmids
HU194307B (en) * 1983-09-16 1988-01-28 Richter Gedeon Vegyeszet Process for producing plasmidvectors of high copy number
EP0151760A1 (en) * 1984-01-06 1985-08-21 Takeda Chemical Industries, Ltd. Novel DNA and use thereof
GB2166743B (en) * 1984-11-13 1989-01-05 Solvay Process for a thermo-inducible gene expression in gram positive bacteria and bacillus subtilis strains containing plasmids which induce such an expression

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2759053A1 (de) * 1977-12-30 1979-07-12 Uhlin Verfahren zum herstellen von genprodukten von plasmid-dns

Also Published As

Publication number Publication date
DK541979A (da) 1980-06-27
FI793884A (fi) 1980-06-27
EP0013830A2 (en) 1980-08-06
EP0013830A3 (en) 1980-11-12
DE2964664D1 (en) 1983-03-03
ES487236A0 (es) 1980-12-16
JPS55104888A (en) 1980-08-11
EP0013830B1 (en) 1983-01-26
NO794133L (no) 1980-06-27
AU531722B2 (en) 1983-09-01
AU5392279A (en) 1980-07-03
ATE2340T1 (de) 1983-02-15

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