ES433961A1 - Method for the purification and recovery of urokinase - Google Patents

Method for the purification and recovery of urokinase

Info

Publication number
ES433961A1
ES433961A1 ES433961A ES433961A ES433961A1 ES 433961 A1 ES433961 A1 ES 433961A1 ES 433961 A ES433961 A ES 433961A ES 433961 A ES433961 A ES 433961A ES 433961 A1 ES433961 A1 ES 433961A1
Authority
ES
Spain
Prior art keywords
urokinase
solution
neutral
agaropectin
aqueous
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES433961A
Other languages
Spanish (es)
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mitsubishi Tanabe Pharma Corp
Original Assignee
Green Cross Corp Japan
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from JP1002274A external-priority patent/JPS517751B2/ja
Priority claimed from JP1201674A external-priority patent/JPS517752B2/ja
Application filed by Green Cross Corp Japan filed Critical Green Cross Corp Japan
Publication of ES433961A1 publication Critical patent/ES433961A1/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6462—Plasminogen activators u-Plasminogen activator (3.4.21.73), i.e. urokinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21073—Serine endopeptidases (3.4.21) u-Plasminogen activator (3.4.21.73), i.e. urokinase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

Urokinase of a high degree of purity is obtained in good yield by adsorption of the urokinase from a contaminated aqueous urokinase solution, e.g. human urine, on an agaropectin-containing water-insoluble polysaccharide, e.g. agar or an agarose, and agaropectin-containing preparation. The polysaccharide is then removed at a weakly acidic to neutral pH, which is set, e.g., using a buffered salt solution, with the urokinase adsorbed thereon by ion formation. After washing out the impurities which may be present, the urokinase is eluted in an ion exchange reaction using an aqueous alkaline solution or a concentrated aqueous, neutral solution of a salt or a neutral amino acid. Purified urokinase is used clinically to a large extent for the therapy of peripheral arterial and venous thromboses and myocardial infarcts, which can be attributed to the formation of fibrin clots in blood vessels.
ES433961A 1974-01-22 1975-01-20 Method for the purification and recovery of urokinase Expired ES433961A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP1002274A JPS517751B2 (en) 1974-01-22 1974-01-22
JP1201674A JPS517752B2 (en) 1974-01-28 1974-01-28

Publications (1)

Publication Number Publication Date
ES433961A1 true ES433961A1 (en) 1976-11-16

Family

ID=26345171

Family Applications (1)

Application Number Title Priority Date Filing Date
ES433961A Expired ES433961A1 (en) 1974-01-22 1975-01-20 Method for the purification and recovery of urokinase

Country Status (10)

Country Link
AT (1) AT341079B (en)
AU (1) AU474513B2 (en)
CA (1) CA1028262A (en)
CH (1) CH611314A5 (en)
DE (1) DE2502095C3 (en)
ES (1) ES433961A1 (en)
FR (1) FR2258396B1 (en)
GB (1) GB1476092A (en)
IN (1) IN139029B (en)
SE (1) SE434956B (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2444465A1 (en) * 1978-12-22 1980-07-18 Green Cross Corp Antithrombotic agent contg. urokinase - produced by maintaining pH neutral or weakly alkaline during each processing step for urokinase prodn.

Also Published As

Publication number Publication date
DE2502095A1 (en) 1975-07-24
DE2502095B2 (en) 1978-06-29
IN139029B (en) 1976-04-24
ATA43475A (en) 1977-05-15
CA1028262A (en) 1978-03-21
AT341079B (en) 1978-01-25
AU474513B2 (en) 1976-07-22
AU7688374A (en) 1976-06-24
SE434956B (en) 1984-08-27
SE7500629L (en) 1975-07-23
FR2258396B1 (en) 1977-11-18
CH611314A5 (en) 1979-05-31
FR2258396A1 (en) 1975-08-18
GB1476092A (en) 1977-06-10
DE2502095C3 (en) 1979-02-22

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