ES2872048T3 - Métodos celulares para acoplar interacciones de proteínas y selección y diversificación de moléculas de unión - Google Patents
Métodos celulares para acoplar interacciones de proteínas y selección y diversificación de moléculas de unión Download PDFInfo
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/1024—In vivo mutagenesis using high mutation rate "mutator" host strains by inserting genetic material, e.g. encoding an error prone polymerase, disrupting a gene for mismatch repair
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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- G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
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- G—PHYSICS
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- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
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| PT3247728T (pt) | 2015-01-20 | 2020-07-16 | Igm Biosciences Inc | Moléculas de ligação do recetor da superfamília do fator de necrose tumoral (fnt) e utilização das mesmas |
| KR102357312B1 (ko) | 2015-03-04 | 2022-02-03 | 아이쥐엠 바이오사이언스 인코포레이티드 | Ｃｄ20 결합 분자 및 그의 용도 |
| PT3355913T (pt) | 2015-09-30 | 2024-12-18 | Igm Biosciences Inc | Moléculas de ligação com cadeia j modificada |
| ES2819870T3 (es) | 2015-09-30 | 2021-04-19 | Igm Biosciences Inc | Moléculas de unión con cadena J modificada |
| GB201704115D0 (en) * | 2017-03-15 | 2017-04-26 | Oxford Genetics Ltd | Method of selecting for antibodies |
| CN113388638A (zh) * | 2021-04-13 | 2021-09-14 | 中国人民解放军西部战区总医院 | 一种同时结合TGFβ和VEGF的双靶点融合蛋白质粒的构建方法 |
| EP4463572A4 (en) * | 2022-01-13 | 2026-04-08 | Massachusetts Inst Technology | ANALYSIS, SCREENING AND SELECTION OF FUNCTIONS OF SOLUBBLE PROTEINS IN CELLULAR BANK OF SECRETED PROTEINS |
| JP2025516055A (ja) * | 2022-05-03 | 2025-05-26 | エムブレース セラピューティクス，インコーポレーテッド | 組換え抗体の産生方法及び関連する細胞系 |
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| US20260126450A1 (en) * | 2022-10-12 | 2026-05-07 | Massachusetts Institute Of Technology | Library-scale methods for polypeptide functional analysis |
| CN116236584B (zh) * | 2023-02-20 | 2024-03-22 | 四川大学 | 一种用于高效递送siRNA的多糖-多肽偶联物 |
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| US9518254B2 (en) * | 2009-05-04 | 2016-12-13 | San Diego State University Research Foundation | Compositions and methods for identifying enzyme and transport protein inhibitors |
| AU2010249470B2 (en) * | 2009-05-20 | 2015-06-25 | Novimmune S.A. | Synthetic Polypeptide Libraries And Methods For Generating Naturally Diversified Polypeptide Variants |
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| PT3588089T (pt) | 2021-06-02 |
| EP2962103A2 (en) | 2016-01-06 |
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| EP3588089B1 (en) | 2021-03-10 |
| US9487773B2 (en) | 2016-11-08 |
| US20140249044A1 (en) | 2014-09-04 |
| EP3588089A1 (en) | 2020-01-01 |
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