ES2858527T3 - Lactasa purificada - Google Patents
Lactasa purificada Download PDFInfo
- Publication number
- ES2858527T3 ES2858527T3 ES10176987T ES10176987T ES2858527T3 ES 2858527 T3 ES2858527 T3 ES 2858527T3 ES 10176987 T ES10176987 T ES 10176987T ES 10176987 T ES10176987 T ES 10176987T ES 2858527 T3 ES2858527 T3 ES 2858527T3
- Authority
- ES
- Spain
- Prior art keywords
- lactase
- solution
- lactase solution
- poly
- oligosaccharides
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 102100026189 Beta-galactosidase Human genes 0.000 title claims abstract description 125
- 108010005774 beta-Galactosidase Proteins 0.000 title claims abstract description 125
- 108010059881 Lactase Proteins 0.000 title claims abstract description 121
- 229940116108 lactase Drugs 0.000 title claims abstract description 121
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims abstract description 42
- 238000000034 method Methods 0.000 claims abstract description 38
- 230000008569 process Effects 0.000 claims abstract description 33
- 150000004676 glycans Polymers 0.000 claims abstract description 32
- 229920001542 oligosaccharide Polymers 0.000 claims abstract description 22
- 150000002482 oligosaccharides Chemical class 0.000 claims abstract description 22
- 150000004804 polysaccharides Polymers 0.000 claims abstract description 22
- 238000004519 manufacturing process Methods 0.000 claims abstract description 21
- 235000013336 milk Nutrition 0.000 claims abstract description 21
- 239000008267 milk Substances 0.000 claims abstract description 21
- 210000004080 milk Anatomy 0.000 claims abstract description 21
- 229920001282 polysaccharide Polymers 0.000 claims abstract description 19
- 230000001954 sterilising effect Effects 0.000 claims abstract description 10
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 10
- 238000001914 filtration Methods 0.000 claims abstract description 8
- 239000002904 solvent Substances 0.000 claims abstract description 6
- 241000235649 Kluyveromyces Species 0.000 claims abstract description 5
- 239000003957 anion exchange resin Substances 0.000 claims abstract description 5
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 claims abstract description 4
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical group OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 claims abstract description 4
- 230000002209 hydrophobic effect Effects 0.000 claims abstract description 4
- 239000000600 sorbitol Substances 0.000 claims abstract description 4
- 239000003729 cation exchange resin Substances 0.000 claims abstract description 3
- 230000003993 interaction Effects 0.000 claims abstract description 3
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 claims abstract 2
- 239000000243 solution Substances 0.000 claims description 65
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 claims description 9
- 239000008101 lactose Substances 0.000 claims description 9
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 6
- 239000008103 glucose Substances 0.000 claims description 6
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 4
- 239000000654 additive Substances 0.000 claims description 4
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims description 3
- 239000011780 sodium chloride Substances 0.000 claims description 3
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 claims description 2
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 claims description 2
- 229930195725 Mannitol Natural products 0.000 claims description 2
- 239000007864 aqueous solution Substances 0.000 claims description 2
- 239000000872 buffer Substances 0.000 claims description 2
- 239000000594 mannitol Substances 0.000 claims description 2
- 235000010355 mannitol Nutrition 0.000 claims description 2
- 230000000996 additive effect Effects 0.000 claims 2
- 230000000087 stabilizing effect Effects 0.000 claims 1
- 150000001875 compounds Chemical class 0.000 description 13
- 239000011347 resin Substances 0.000 description 13
- 229920005989 resin Polymers 0.000 description 13
- 108090000790 Enzymes Proteins 0.000 description 12
- 102000004190 Enzymes Human genes 0.000 description 12
- 229940088598 enzyme Drugs 0.000 description 12
- 239000005017 polysaccharide Substances 0.000 description 10
- 238000002360 preparation method Methods 0.000 description 7
- 230000000694 effects Effects 0.000 description 6
- 238000004191 hydrophobic interaction chromatography Methods 0.000 description 6
- 238000000746 purification Methods 0.000 description 6
- 239000000523 sample Substances 0.000 description 6
- 238000004587 chromatography analysis Methods 0.000 description 5
- 238000000926 separation method Methods 0.000 description 5
- 235000013365 dairy product Nutrition 0.000 description 4
- 238000010828 elution Methods 0.000 description 4
- 238000000855 fermentation Methods 0.000 description 4
- 230000004151 fermentation Effects 0.000 description 4
- 238000009928 pasteurization Methods 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 235000002639 sodium chloride Nutrition 0.000 description 4
- 238000011146 sterile filtration Methods 0.000 description 4
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 238000007792 addition Methods 0.000 description 3
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 3
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 3
- 235000011130 ammonium sulphate Nutrition 0.000 description 3
- 239000007857 degradation product Substances 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 238000004128 high performance liquid chromatography Methods 0.000 description 3
- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 description 3
- 238000011084 recovery Methods 0.000 description 3
- 238000003860 storage Methods 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 241001138401 Kluyveromyces lactis Species 0.000 description 2
- 241000124008 Mammalia Species 0.000 description 2
- 229920002684 Sepharose Polymers 0.000 description 2
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 2
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 2
- 239000007983 Tris buffer Substances 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- WQZGKKKJIJFFOK-PHYPRBDBSA-N alpha-D-galactose Chemical compound OC[C@H]1O[C@H](O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-PHYPRBDBSA-N 0.000 description 2
- 150000001450 anions Chemical class 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000011210 chromatographic step Methods 0.000 description 2
- 239000000470 constituent Substances 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- 238000003795 desorption Methods 0.000 description 2
- 150000002016 disaccharides Chemical class 0.000 description 2
- 229930182830 galactose Natural products 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 229910052588 hydroxylapatite Inorganic materials 0.000 description 2
- 235000020191 long-life milk Nutrition 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 235000020200 pasteurised milk Nutrition 0.000 description 2
- XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical compound [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 229910052938 sodium sulfate Inorganic materials 0.000 description 2
- 235000011152 sodium sulphate Nutrition 0.000 description 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
- 238000005303 weighing Methods 0.000 description 2
- PKAUICCNAWQPAU-UHFFFAOYSA-N 2-(4-chloro-2-methylphenoxy)acetic acid;n-methylmethanamine Chemical compound CNC.CC1=CC(Cl)=CC=C1OCC(O)=O PKAUICCNAWQPAU-UHFFFAOYSA-N 0.000 description 1
- 229930091371 Fructose Natural products 0.000 description 1
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
- 239000005715 Fructose Substances 0.000 description 1
- 241000235650 Kluyveromyces marxianus Species 0.000 description 1
- 201000010538 Lactose Intolerance Diseases 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 239000012614 Q-Sepharose Substances 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 241000235070 Saccharomyces Species 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 125000000484 butyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 238000005341 cation exchange Methods 0.000 description 1
- 229940023913 cation exchange resins Drugs 0.000 description 1
- 150000001768 cations Chemical class 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 239000013065 commercial product Substances 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 210000002249 digestive system Anatomy 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 239000006167 equilibration buffer Substances 0.000 description 1
- 125000001495 ethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 238000002523 gelfiltration Methods 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 208000028774 intestinal disease Diseases 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 238000004255 ion exchange chromatography Methods 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 150000002772 monosaccharides Chemical class 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 125000001209 o-nitrophenyl group Chemical group [H]C1=C([H])C(*)=C(C([H])=C1[H])[N+]([O-])=O 0.000 description 1
- 125000002347 octyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 235000016046 other dairy product Nutrition 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- -1 polysaccharides Chemical class 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 125000001436 propyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 238000011012 sanitization Methods 0.000 description 1
- 235000020183 skimmed milk Nutrition 0.000 description 1
- 229910000029 sodium carbonate Inorganic materials 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 239000012086 standard solution Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 150000004043 trisaccharides Chemical class 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING OR TREATMENT THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
- A23C9/1203—Addition of, or treatment with, enzymes or microorganisms other than lactobacteriaceae
- A23C9/1206—Lactose hydrolysing enzymes, e.g. lactase, beta-galactosidase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2468—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on beta-galactose-glycoside bonds, e.g. carrageenases (3.2.1.83; 3.2.1.157); beta-agarase (3.2.1.81)
- C12N9/2471—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01023—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING OR TREATMENT THEREOF
- A23C2210/00—Physical treatment of dairy products
- A23C2210/20—Treatment using membranes, including sterile filtration
- A23C2210/208—Removal of bacteria by membrane filtration; Sterile filtration of milk products
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01108—Lactase (3.2.1.108)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Dairy Products (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP01000102 | 2001-04-04 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2858527T3 true ES2858527T3 (es) | 2021-09-30 |
Family
ID=8176033
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES10176987T Expired - Lifetime ES2858527T3 (es) | 2001-04-04 | 2002-04-03 | Lactasa purificada |
| ES02759791.3T Expired - Lifetime ES2564821T3 (es) | 2001-04-04 | 2002-04-03 | Lactasa purificada |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES02759791.3T Expired - Lifetime ES2564821T3 (es) | 2001-04-04 | 2002-04-03 | Lactasa purificada |
Country Status (8)
| Country | Link |
|---|---|
| US (3) | US7955831B2 (enExample) |
| EP (2) | EP1373491B1 (enExample) |
| JP (6) | JP2004534527A (enExample) |
| CN (1) | CN100447242C (enExample) |
| DK (2) | DK2280065T3 (enExample) |
| ES (2) | ES2858527T3 (enExample) |
| PT (2) | PT1373491E (enExample) |
| WO (1) | WO2002081673A1 (enExample) |
Families Citing this family (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2004534527A (ja) | 2001-04-04 | 2004-11-18 | デーエスエム イーペー アセッツ ベスローテン フェンノートシャップ | 精製ラクターゼ |
| AU2013200779C1 (en) * | 2005-11-28 | 2022-11-10 | Dsm Ip Assets B.V. | Enzyme preparations yielding a clean taste |
| ES2454167T3 (es) * | 2005-11-28 | 2014-04-09 | Dsm Ip Assets B.V. | Preparación de enzima que produce un sabor puro |
| US8236297B2 (en) * | 2007-10-11 | 2012-08-07 | Kenneth Manzo | Method of treating lactose intolerance using genetically engineered bacteria |
| WO2009071539A1 (en) | 2007-12-03 | 2009-06-11 | Novozymes A/S | Method for producing a dairy product |
| EP2272374B1 (en) | 2009-06-23 | 2015-01-14 | Nestec S.A. | Lactase containing milk powder |
| WO2012124668A1 (ja) * | 2011-03-14 | 2012-09-20 | 合同酒精株式会社 | アリールスルファターゼの活性測定方法 |
| WO2014185364A1 (ja) | 2013-05-13 | 2014-11-20 | 合同酒精株式会社 | ラクターゼ含有組成物の製造法 |
| MX374800B (es) * | 2014-03-05 | 2025-03-06 | Dsm Ip Assets Bv | Composiciones líquidas de lactasa. |
| US20170245513A1 (en) * | 2014-08-27 | 2017-08-31 | Godo Shusei Co., Ltd. | Lactase solution and milk using same |
| DK3208332T3 (da) * | 2014-10-17 | 2020-03-30 | Godo Shusei Kk | Laktaseopløsning og mælk med samme |
| MX2017007197A (es) | 2014-12-05 | 2017-08-28 | Godo Shusei Kk | Solucion de lactasa y producto lacteo que usa la misma. |
| AR107872A1 (es) | 2016-03-16 | 2018-06-13 | Godo Sushei Co Ltd | Proteinasa b y solución de lactasa usando sus propiedades y método para producirlo |
| CN110167352A (zh) * | 2017-01-13 | 2019-08-23 | 诺维信公司 | 包含具有一价阳离子的盐的无菌过滤的乳糖酶制剂 |
| WO2018225623A1 (ja) * | 2017-06-07 | 2018-12-13 | 天野エンザイム株式会社 | ラクターゼ原末及びラクターゼ製剤 |
| CN107858342B (zh) * | 2017-10-11 | 2020-09-25 | 华中农业大学 | 一种稳定的乳糖酶液态制剂 |
| JP7571141B2 (ja) | 2019-12-26 | 2024-10-22 | ファームユニオン エルエルシー | 安定的ラクターゼ製品 |
| CN115443336A (zh) * | 2020-04-17 | 2022-12-06 | 合同酒精株式会社 | 容器装乳糖酶溶液 |
| WO2023038057A1 (ja) | 2021-09-07 | 2023-03-16 | 合同酒精株式会社 | ラクターゼ、ラクターゼ製剤、遺伝子、組み換えベクターおよび形質転換体 |
| EP4446409A4 (en) | 2021-12-09 | 2025-12-03 | Godo Shusei Kk | LACTASE SOLUTION |
Family Cites Families (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1306751A (en) * | 1971-01-26 | 1973-02-14 | Baxter Laboratories Inc | Lactase enzyme production |
| US3753725A (en) | 1971-03-11 | 1973-08-21 | R Williams | Method for enzymatic conversion of lactose to glucose and galactose in lactose containing fluids |
| US3919049A (en) | 1974-06-27 | 1975-11-11 | Tokyo Tanabe Co | Process for preparing {62 -galactosidase |
| SE438773B (sv) | 1974-11-13 | 1985-05-13 | Tetra Pak Int | Sett att framstella en steril mejeriprodukt med nedsatt halt av laktos |
| US4007283A (en) | 1975-10-28 | 1977-02-08 | The Regents Of The University Of California | Production and utilization of lactase |
| JPS53148591A (en) * | 1977-05-31 | 1978-12-25 | Daiwa Kasei Kk | Production of novel lactase |
| JPS5571445A (en) | 1978-11-24 | 1980-05-29 | Morinaga Milk Ind Co Ltd | Production of lactose hydrolyzate |
| EP0014502B1 (en) * | 1979-02-06 | 1983-07-27 | THE PROCTER & GAMBLE COMPANY | Soap bars |
| JPS5651984A (en) | 1979-10-02 | 1981-05-09 | Sumitomo Chem Co Ltd | Fixed lactase and its preparation |
| JPS56140890A (en) | 1980-04-04 | 1981-11-04 | Sumitomo Chem Co Ltd | Immobilized lactase and its preparation |
| FR2500269A1 (fr) | 1981-02-20 | 1982-08-27 | Tetra Pak Int | Procede pour le traitement des produits laitiers pour diminuer leur teneur en lactose notamment |
| JPS59175879A (ja) | 1983-03-25 | 1984-10-04 | Snow Brand Milk Prod Co Ltd | 酵素における汚染細菌の殺菌法 |
| AU586809B2 (en) * | 1983-12-02 | 1989-07-27 | Gist-Brocades N.V. | Process for the preparation of a germ free solution of a lactose-splitting enzyme and its use. |
| US5283189A (en) | 1987-02-09 | 1994-02-01 | Research Development Corporation Of Japan | β-galactosidase from Thermus sp |
| US5357852A (en) | 1992-06-02 | 1994-10-25 | Kohler Mix Specialties | Method and apparatus for producing reduced lactose milk |
| US5744345A (en) * | 1992-10-05 | 1998-04-28 | Takara Shuzo Co., Ltd. | Hyperthermostable β-galactosidase gene, enzyme encoded thereby, and process for production |
| US5821350A (en) | 1995-11-01 | 1998-10-13 | Nexia Biotechnologies, Inc. | Aspergillus niger beta-galactosidase gene |
| ES2130991B1 (es) | 1997-05-16 | 2000-04-01 | Plumley Kevin More | Procedimiento que implica la pre-digestion parcial en una linea de produccion para la elaboracion de leche y derivados lacteos libres de lactosa para evitar descargas de 5-hidroxi-triptamina - o 5-ht. |
| US6146902A (en) | 1998-12-29 | 2000-11-14 | Aventis Pasteur, Inc. | Purification of polysaccharide-protein conjugate vaccines by ultrafiltration with ammonium sulfate solutions |
| JP2003504048A (ja) * | 1999-07-09 | 2003-02-04 | ユニベルシテ ドゥ リエージュ | 低温活性ベータ・ガラクトシダーゼ、その製法、及びその使用 |
| JP2004534527A (ja) * | 2001-04-04 | 2004-11-18 | デーエスエム イーペー アセッツ ベスローテン フェンノートシャップ | 精製ラクターゼ |
-
2002
- 2002-04-03 JP JP2002580036A patent/JP2004534527A/ja active Pending
- 2002-04-03 ES ES10176987T patent/ES2858527T3/es not_active Expired - Lifetime
- 2002-04-03 US US10/474,113 patent/US7955831B2/en not_active Expired - Lifetime
- 2002-04-03 PT PT2759791T patent/PT1373491E/pt unknown
- 2002-04-03 CN CNB028072863A patent/CN100447242C/zh not_active Ceased
- 2002-04-03 EP EP02759791.3A patent/EP1373491B1/en not_active Revoked
- 2002-04-03 WO PCT/EP2002/003680 patent/WO2002081673A1/en not_active Ceased
- 2002-04-03 DK DK10176987.5T patent/DK2280065T3/da active
- 2002-04-03 PT PT101769875T patent/PT2280065T/pt unknown
- 2002-04-03 EP EP10176987.5A patent/EP2280065B1/en not_active Expired - Lifetime
- 2002-04-03 ES ES02759791.3T patent/ES2564821T3/es not_active Expired - Lifetime
- 2002-04-03 DK DK02759791.3T patent/DK1373491T3/en active
-
2008
- 2008-11-19 JP JP2008295983A patent/JP2009034116A/ja active Pending
-
2010
- 2010-02-22 JP JP2010036310A patent/JP2010148516A/ja active Pending
-
2011
- 2011-05-05 US US13/067,065 patent/US9149048B2/en not_active Expired - Lifetime
-
2013
- 2013-02-27 JP JP2013036972A patent/JP2013099364A/ja active Pending
- 2013-10-24 JP JP2013221129A patent/JP2014050399A/ja active Pending
-
2015
- 2015-08-27 US US14/837,275 patent/US9763460B2/en not_active Expired - Fee Related
- 2015-10-01 JP JP2015196009A patent/JP2016000058A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| US9149048B2 (en) | 2015-10-06 |
| JP2004534527A (ja) | 2004-11-18 |
| JP2013099364A (ja) | 2013-05-23 |
| US20150359239A1 (en) | 2015-12-17 |
| US9763460B2 (en) | 2017-09-19 |
| EP1373491B1 (en) | 2015-12-30 |
| JP2016000058A (ja) | 2016-01-07 |
| WO2002081673A1 (en) | 2002-10-17 |
| PT1373491E (pt) | 2016-03-31 |
| DK1373491T3 (en) | 2016-03-14 |
| US20040121041A1 (en) | 2004-06-24 |
| US7955831B2 (en) | 2011-06-07 |
| EP1373491A1 (en) | 2004-01-02 |
| EP2280065B1 (en) | 2021-01-13 |
| JP2010148516A (ja) | 2010-07-08 |
| DK2280065T3 (da) | 2021-03-29 |
| CN100447242C (zh) | 2008-12-31 |
| ES2564821T3 (es) | 2016-03-29 |
| EP2280065A1 (en) | 2011-02-02 |
| PT2280065T (pt) | 2021-02-16 |
| CN1500143A (zh) | 2004-05-26 |
| JP2014050399A (ja) | 2014-03-20 |
| US20110212221A1 (en) | 2011-09-01 |
| JP2009034116A (ja) | 2009-02-19 |
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