ES2853351T3 - Identificación y aislamiento de células endoteliales corneales humanas (HCECs) - Google Patents

Identificación y aislamiento de células endoteliales corneales humanas (HCECs) Download PDF

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Publication number
ES2853351T3
ES2853351T3 ES14792047T ES14792047T ES2853351T3 ES 2853351 T3 ES2853351 T3 ES 2853351T3 ES 14792047 T ES14792047 T ES 14792047T ES 14792047 T ES14792047 T ES 14792047T ES 2853351 T3 ES2853351 T3 ES 2853351T3
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ES
Spain
Prior art keywords
affinity reagent
cells
positive
antibody
hcecs
Prior art date
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Active
Application number
ES14792047T
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English (en)
Spanish (es)
Inventor
Jeffrey Goldberg
Noelia Kunzevitzky
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
EMMETROPE OPHTHALMICS LLC
Original Assignee
EMMETROPE OPHTHALMICS LLC
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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0621—Eye cells, e.g. cornea, iris pigmented cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5064—Endothelial cells

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Neurology (AREA)
  • Neurosurgery (AREA)
  • General Engineering & Computer Science (AREA)
  • Ophthalmology & Optometry (AREA)
  • Toxicology (AREA)
  • Virology (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Materials For Medical Uses (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Prostheses (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
ES14792047T 2013-05-03 2014-05-02 Identificación y aislamiento de células endoteliales corneales humanas (HCECs) Active ES2853351T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201361819146P 2013-05-03 2013-05-03
PCT/US2014/036616 WO2014179716A2 (en) 2013-05-03 2014-05-02 IDENTIFICATION AND ISOLATION OF HUMAN CORNEAL ENDOTHELIAL CELLS (HCECs)

Publications (1)

Publication Number Publication Date
ES2853351T3 true ES2853351T3 (es) 2021-09-15

Family

ID=51844125

Family Applications (1)

Application Number Title Priority Date Filing Date
ES14792047T Active ES2853351T3 (es) 2013-05-03 2014-05-02 Identificación y aislamiento de células endoteliales corneales humanas (HCECs)

Country Status (5)

Country Link
US (3) US10655102B2 (enExample)
EP (2) EP3985102B1 (enExample)
JP (4) JP6525962B2 (enExample)
ES (1) ES2853351T3 (enExample)
WO (1) WO2014179716A2 (enExample)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA3178138A1 (en) 2011-12-06 2013-06-13 Astellas Institute For Regenerative Medicine Method of directed differentiation producing corneal endothelial cells, compositions thereof, and uses thereof
ES2853351T3 (es) * 2013-05-03 2021-09-15 Emmetrope Ophthalmics Llc Identificación y aislamiento de células endoteliales corneales humanas (HCECs)

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1130032A1 (en) 2000-02-28 2001-09-05 Gesellschaft für biotechnologische Forschung mbH (GBF) Single-chain antibodies recognizing the human vascular endothelial growth factor receptor-2 (VEGFR-2/KDR)
CN1898377A (zh) 2003-10-10 2007-01-17 细胞生物工程公司 人角膜内皮细胞以及获得和培养用于角膜细胞移植的细胞的方法
CN102224171B (zh) * 2008-09-25 2015-03-11 国家健康与医学研究院 抑制丙型肝炎病毒感染的单克隆抗紧密连接蛋白-1抗体
EP2383334A4 (en) * 2009-01-23 2013-01-16 Univ Osaka FEEDER CELL FOR TARGET CELL INDUCTION
JP5835693B2 (ja) * 2010-02-05 2015-12-24 株式会社角膜再生研究所 角膜内皮細胞の培養方法、移植用角膜内皮細胞シートの製造方法および角膜内皮細胞培養キット
US9347041B2 (en) * 2011-07-15 2016-05-24 Osaka University Method for preparing corneal endothelial cell
CA3178138A1 (en) * 2011-12-06 2013-06-13 Astellas Institute For Regenerative Medicine Method of directed differentiation producing corneal endothelial cells, compositions thereof, and uses thereof
ES2853351T3 (es) * 2013-05-03 2021-09-15 Emmetrope Ophthalmics Llc Identificación y aislamiento de células endoteliales corneales humanas (HCECs)
WO2015016371A1 (ja) 2013-07-30 2015-02-05 京都府公立大学法人 角膜内皮細胞マーカー

Also Published As

Publication number Publication date
JP2021112206A (ja) 2021-08-05
JP7752485B2 (ja) 2025-10-10
US20200277572A1 (en) 2020-09-03
WO2014179716A2 (en) 2014-11-06
EP3985102A1 (en) 2022-04-20
US11740239B2 (en) 2023-08-29
EP2992087A2 (en) 2016-03-09
US20240027446A1 (en) 2024-01-25
JP6877483B2 (ja) 2021-05-26
WO2014179716A3 (en) 2015-01-22
EP2992087A4 (en) 2016-09-21
EP2992087B1 (en) 2020-12-30
US20160102290A1 (en) 2016-04-14
JP2023116581A (ja) 2023-08-22
EP3985102B1 (en) 2025-10-15
JP2016521130A (ja) 2016-07-21
JP2019150039A (ja) 2019-09-12
JP6525962B2 (ja) 2019-06-05
US10655102B2 (en) 2020-05-19
EP3985102C0 (en) 2025-10-15

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