ES2853351T3 - Identificación y aislamiento de células endoteliales corneales humanas (HCECs) - Google Patents

Identificación y aislamiento de células endoteliales corneales humanas (HCECs) Download PDF

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Publication number
ES2853351T3
ES2853351T3 ES14792047T ES14792047T ES2853351T3 ES 2853351 T3 ES2853351 T3 ES 2853351T3 ES 14792047 T ES14792047 T ES 14792047T ES 14792047 T ES14792047 T ES 14792047T ES 2853351 T3 ES2853351 T3 ES 2853351T3
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Spain
Prior art keywords
affinity reagent
cells
positive
antibody
hcecs
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES14792047T
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English (en)
Spanish (es)
Inventor
Jeffrey Goldberg
Noelia Kunzevitzky
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
EMMETROPE OPHTHALMICS LLC
Original Assignee
EMMETROPE OPHTHALMICS LLC
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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0621—Eye cells, e.g. cornea, iris pigmented cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5064—Endothelial cells

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Ophthalmology & Optometry (AREA)
  • Neurology (AREA)
  • Neurosurgery (AREA)
  • General Engineering & Computer Science (AREA)
  • Toxicology (AREA)
  • Virology (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Materials For Medical Uses (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Prostheses (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
ES14792047T 2013-05-03 2014-05-02 Identificación y aislamiento de células endoteliales corneales humanas (HCECs) Active ES2853351T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201361819146P 2013-05-03 2013-05-03
PCT/US2014/036616 WO2014179716A2 (en) 2013-05-03 2014-05-02 IDENTIFICATION AND ISOLATION OF HUMAN CORNEAL ENDOTHELIAL CELLS (HCECs)

Publications (1)

Publication Number Publication Date
ES2853351T3 true ES2853351T3 (es) 2021-09-15

Family

ID=51844125

Family Applications (1)

Application Number Title Priority Date Filing Date
ES14792047T Active ES2853351T3 (es) 2013-05-03 2014-05-02 Identificación y aislamiento de células endoteliales corneales humanas (HCECs)

Country Status (5)

Country Link
US (3) US10655102B2 (enExample)
EP (2) EP3985102B1 (enExample)
JP (4) JP6525962B2 (enExample)
ES (1) ES2853351T3 (enExample)
WO (1) WO2014179716A2 (enExample)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TR201903889T4 (tr) 2011-12-06 2019-04-22 Astellas Inst For Regenerative Medicine Korneal endotelyal hücreler üreten yönlendirilmiş diferansiyasyona yönelik yöntem.
EP3985102B1 (en) * 2013-05-03 2025-10-15 Emmetrope Ophthalmics LLC Identification and isolation of human corneal endothelial cells (hcecs)

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1130032A1 (en) * 2000-02-28 2001-09-05 Gesellschaft für biotechnologische Forschung mbH (GBF) Single-chain antibodies recognizing the human vascular endothelial growth factor receptor-2 (VEGFR-2/KDR)
CA2542133A1 (en) 2003-10-10 2005-04-28 Cellular Bioengineering, Inc. Human corneal endothelial cells and methods of obtaining and culturing cells for corneal cell transplantation
BRPI0919065B1 (pt) * 2008-09-25 2021-09-21 INSERM (Institut National de la Santé et de la Recherche Médicale) Anticorpos monoclonais anti-claudin-i para a inibição de infecção por vírus de hepatite c
WO2010084970A1 (ja) * 2009-01-23 2010-07-29 国立大学法人大阪大学 標的細胞誘導用フィーダー細胞
WO2011096593A1 (ja) * 2010-02-05 2011-08-11 財団法人先端医療振興財団 角膜内皮細胞の培養方法、移植用角膜内皮細胞シートの製造方法および角膜内皮細胞培養キット
JP5761827B2 (ja) 2011-07-15 2015-08-12 国立大学法人大阪大学 角膜内皮細胞の調製方法
TR201903889T4 (tr) 2011-12-06 2019-04-22 Astellas Inst For Regenerative Medicine Korneal endotelyal hücreler üreten yönlendirilmiş diferansiyasyona yönelik yöntem.
EP3985102B1 (en) * 2013-05-03 2025-10-15 Emmetrope Ophthalmics LLC Identification and isolation of human corneal endothelial cells (hcecs)
US10908161B2 (en) 2013-07-30 2021-02-02 Kyoto Prefectural Public University Corporation Corneal endothelial cell marker

Also Published As

Publication number Publication date
EP3985102B1 (en) 2025-10-15
JP2023116581A (ja) 2023-08-22
EP2992087A4 (en) 2016-09-21
EP2992087B1 (en) 2020-12-30
JP6525962B2 (ja) 2019-06-05
JP2019150039A (ja) 2019-09-12
JP2021112206A (ja) 2021-08-05
US20200277572A1 (en) 2020-09-03
EP2992087A2 (en) 2016-03-09
EP3985102C0 (en) 2025-10-15
US20160102290A1 (en) 2016-04-14
WO2014179716A2 (en) 2014-11-06
US10655102B2 (en) 2020-05-19
JP7752485B2 (ja) 2025-10-10
US20240027446A1 (en) 2024-01-25
US11740239B2 (en) 2023-08-29
JP2016521130A (ja) 2016-07-21
EP3985102A1 (en) 2022-04-20
JP6877483B2 (ja) 2021-05-26
WO2014179716A3 (en) 2015-01-22

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