ES2823151T3 - Método, sistema y producto del programa informático para determinar la presencia de microorganismos e identificar dichos microorganismos - Google Patents

Método, sistema y producto del programa informático para determinar la presencia de microorganismos e identificar dichos microorganismos Download PDF

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Publication number
ES2823151T3
ES2823151T3 ES17758553T ES17758553T ES2823151T3 ES 2823151 T3 ES2823151 T3 ES 2823151T3 ES 17758553 T ES17758553 T ES 17758553T ES 17758553 T ES17758553 T ES 17758553T ES 2823151 T3 ES2823151 T3 ES 2823151T3
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Spain
Prior art keywords
petri dish
image
processed
colonies
pixels
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Active
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ES17758553T
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English (en)
Spanish (es)
Inventor
Guillaume Sutra
Philippine Barlas
Delphine Archeny
Géraldine Durand
Corine Fulchiron
Jean-François Gorse
Guillaume Perrin
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Biomerieux SA
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Biomerieux SA
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Priority claimed from EP16187153.8A external-priority patent/EP3290524A1/en
Application filed by Biomerieux SA filed Critical Biomerieux SA
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Publication of ES2823151T3 publication Critical patent/ES2823151T3/es
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/045—Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T5/00—Image enhancement or restoration
    • G06T5/20—Image enhancement or restoration using local operators
    • G06T5/30—Erosion or dilatation, e.g. thinning
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T5/00—Image enhancement or restoration
    • G06T5/50—Image enhancement or restoration using two or more images, e.g. averaging or subtraction
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/0002—Inspection of images, e.g. flaw detection
    • G06T7/0012—Biomedical image inspection
    • G06T7/0014—Biomedical image inspection using an image reference approach
    • G06T7/0016—Biomedical image inspection using an image reference approach involving temporal comparison
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/10—Segmentation; Edge detection
    • G06T7/11—Region-based segmentation
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/10—Segmentation; Edge detection
    • G06T7/136—Segmentation; Edge detection involving thresholding
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/10—Segmentation; Edge detection
    • G06T7/162—Segmentation; Edge detection involving graph-based methods
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V20/00—Scenes; Scene-specific elements
    • G06V20/60—Type of objects
    • G06V20/69—Microscopic objects, e.g. biological cells or cellular parts
    • G06V20/695—Preprocessing, e.g. image segmentation
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V20/00—Scenes; Scene-specific elements
    • G06V20/60—Type of objects
    • G06V20/69—Microscopic objects, e.g. biological cells or cellular parts
    • G06V20/698—Matching; Classification
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/10—Image acquisition modality
    • G06T2207/10024—Color image
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/20—Special algorithmic details
    • G06T2207/20036—Morphological image processing
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/20—Special algorithmic details
    • G06T2207/20076—Probabilistic image processing
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/20—Special algorithmic details
    • G06T2207/20212—Image combination
    • G06T2207/20224—Image subtraction
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/30—Subject of image; Context of image processing
    • G06T2207/30004—Biomedical image processing
    • G06T2207/30024—Cell structures in vitro; Tissue sections in vitro

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  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Theoretical Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Toxicology (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Medical Informatics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Radiology & Medical Imaging (AREA)
  • Quality & Reliability (AREA)
  • Biomedical Technology (AREA)
  • Multimedia (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
ES17758553T 2016-09-02 2017-08-30 Método, sistema y producto del programa informático para determinar la presencia de microorganismos e identificar dichos microorganismos Active ES2823151T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP16187153.8A EP3290524A1 (en) 2016-09-02 2016-09-02 Method, system and computer program product for determining the presence of microorganisms and identifying said microorganisms
EP16306113 2016-09-05
PCT/EP2017/071775 WO2018041900A1 (en) 2016-09-02 2017-08-30 Method, system and computer program product for determining the presence of microorganisms and identifying said microorganisms

Publications (1)

Publication Number Publication Date
ES2823151T3 true ES2823151T3 (es) 2021-05-06

Family

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Application Number Title Priority Date Filing Date
ES17758553T Active ES2823151T3 (es) 2016-09-02 2017-08-30 Método, sistema y producto del programa informático para determinar la presencia de microorganismos e identificar dichos microorganismos

Country Status (6)

Country Link
US (1) US10968473B2 (cg-RX-API-DMAC10.html)
EP (1) EP3507378B1 (cg-RX-API-DMAC10.html)
JP (1) JP7007367B2 (cg-RX-API-DMAC10.html)
CN (1) CN109661473B (cg-RX-API-DMAC10.html)
ES (1) ES2823151T3 (cg-RX-API-DMAC10.html)
WO (1) WO2018041900A1 (cg-RX-API-DMAC10.html)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111286523A (zh) * 2020-02-20 2020-06-16 中国水产科学研究院黄海水产研究所 一种细菌的三原色鉴别及计数方法
FR3109159B1 (fr) * 2020-04-09 2023-06-23 Interscience Procédé de traitement d’images appliqué aux compteurs de colonies en microbiologie
CN116263955A (zh) * 2023-01-18 2023-06-16 浙江泰林医学工程有限公司 一种自动菌落计数方法及系统
CN116128845B (zh) * 2023-02-10 2024-05-07 甘肃省科学院生物研究所 一种基于随机漫步的菌落自动计数方法及菌落计数器
CN117557583B (zh) * 2024-01-10 2024-03-15 江苏锡澄环境科学研究院有限公司 基于图像处理的电泳线废水处理效果视觉检测方法
CN117953495B (zh) * 2024-03-27 2024-06-04 北京大学口腔医学院 一种口腔微生物菌群群落分割方法
CN118196087B (zh) * 2024-05-15 2024-08-02 江苏海枫达生物科技有限公司 一种基于图像采集的微生物菌落检测系统及其方法
CN119842475A (zh) * 2025-03-18 2025-04-18 浙江鹰旸医药研发有限公司 一种微生物智能培养识别分析系统及方法

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5694478A (en) * 1994-12-15 1997-12-02 Minnesota Mining And Manufacturing Company Method and apparatus for detecting and identifying microbial colonies
US20020086289A1 (en) * 1999-06-15 2002-07-04 Don Straus Genomic profiling: a rapid method for testing a complex biological sample for the presence of many types of organisms
JP2002125696A (ja) * 2000-10-19 2002-05-08 National Institute Of Advanced Industrial & Technology 媒体中の生細胞の検出および該媒体のｐＨ測定のための方法
JP2003116593A (ja) * 2001-10-17 2003-04-22 Hakuju Inst For Health Science Co Ltd 微生物の判定方法およびその装置
CN1536090A (zh) * 2003-04-07 2004-10-13 中国人民解放军军事医学科学院卫生学 食源性致病菌快速检测基因芯片及其应用
US20040253660A1 (en) * 2003-06-12 2004-12-16 Giles Scientific, Inc. Automated microbiological testing apparatus and method
US20080153125A1 (en) * 2006-11-10 2008-06-26 Advanced Analytical Technologies, Inc. Rapid detection of microorganisms in fluids
EP2250281B1 (en) * 2008-02-19 2015-04-08 Becton Dickinson and Company Systems and methods for presumptive identification of microorganism type in a culture
JP5439096B2 (ja) 2009-09-08 2014-03-12 株式会社日立ハイテクノロジーズ 細菌分類装置および細菌検査前処理装置
EP2520923A1 (en) * 2011-05-06 2012-11-07 bioMérieux Bio-imaging method and system
EP2731051A1 (en) * 2012-11-07 2014-05-14 bioMérieux Bio-imaging method
JP6223932B2 (ja) * 2014-09-02 2017-11-01 株式会社エヌテック 微生物検出方法、微生物検出装置及びプログラム

Also Published As

Publication number Publication date
EP3507378A1 (en) 2019-07-10
US10968473B2 (en) 2021-04-06
CN109661473A (zh) 2019-04-19
EP3507378B1 (en) 2020-07-29
WO2018041900A1 (en) 2018-03-08
CN109661473B (zh) 2022-06-07
JP7007367B2 (ja) 2022-01-24
JP2019531065A (ja) 2019-10-31
US20190203257A1 (en) 2019-07-04

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