ES2798262T3 - Método mejorado para la determinación de microorganismos - Google Patents
Método mejorado para la determinación de microorganismos Download PDFInfo
- Publication number
- ES2798262T3 ES2798262T3 ES15857723T ES15857723T ES2798262T3 ES 2798262 T3 ES2798262 T3 ES 2798262T3 ES 15857723 T ES15857723 T ES 15857723T ES 15857723 T ES15857723 T ES 15857723T ES 2798262 T3 ES2798262 T3 ES 2798262T3
- Authority
- ES
- Spain
- Prior art keywords
- microorganisms
- suspension
- enzyme
- cellulose
- cellulase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 244000005700 microbiome Species 0.000 title claims abstract description 54
- 238000000034 method Methods 0.000 title claims abstract description 43
- 239000000463 material Substances 0.000 claims abstract description 42
- 108090000790 Enzymes Proteins 0.000 claims abstract description 40
- 102000004190 Enzymes Human genes 0.000 claims abstract description 40
- 229940088598 enzyme Drugs 0.000 claims abstract description 40
- 108010059892 Cellulase Proteins 0.000 claims abstract description 35
- 230000000694 effects Effects 0.000 claims abstract description 28
- 229940106157 cellulase Drugs 0.000 claims abstract description 23
- 229920002678 cellulose Polymers 0.000 claims abstract description 22
- 239000001913 cellulose Substances 0.000 claims abstract description 22
- 239000000725 suspension Substances 0.000 claims abstract description 20
- 108020004707 nucleic acids Proteins 0.000 claims abstract description 10
- 150000007523 nucleic acids Chemical class 0.000 claims abstract description 10
- 102000039446 nucleic acids Human genes 0.000 claims abstract description 10
- 238000010222 PCR analysis Methods 0.000 claims abstract description 4
- 229940079919 digestives enzyme preparation Drugs 0.000 claims abstract description 4
- 239000011111 cardboard Substances 0.000 claims description 34
- 239000000123 paper Substances 0.000 claims description 23
- 238000005119 centrifugation Methods 0.000 claims description 17
- 238000002360 preparation method Methods 0.000 claims description 17
- 239000011087 paperboard Substances 0.000 claims description 13
- 241000894006 Bacteria Species 0.000 claims description 10
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 9
- 108010002430 hemicellulase Proteins 0.000 claims description 9
- 239000000203 mixture Substances 0.000 claims description 9
- 235000013305 food Nutrition 0.000 claims description 8
- 229940059442 hemicellulase Drugs 0.000 claims description 5
- 241000233866 Fungi Species 0.000 claims description 2
- 238000003753 real-time PCR Methods 0.000 description 29
- 238000004806 packaging method and process Methods 0.000 description 25
- 239000000523 sample Substances 0.000 description 15
- 230000002255 enzymatic effect Effects 0.000 description 11
- 239000000872 buffer Substances 0.000 description 10
- 238000001914 filtration Methods 0.000 description 10
- 238000004458 analytical method Methods 0.000 description 9
- 230000001580 bacterial effect Effects 0.000 description 9
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 9
- 230000006835 compression Effects 0.000 description 9
- 238000007906 compression Methods 0.000 description 9
- 238000003752 polymerase chain reaction Methods 0.000 description 9
- 239000007788 liquid Substances 0.000 description 8
- 230000000813 microbial effect Effects 0.000 description 8
- DXHWIAMGTKXUEA-UHFFFAOYSA-O propidium monoazide Chemical compound C12=CC(N=[N+]=[N-])=CC=C2C2=CC=C(N)C=C2[N+](CCC[N+](C)(CC)CC)=C1C1=CC=CC=C1 DXHWIAMGTKXUEA-UHFFFAOYSA-O 0.000 description 8
- 238000000926 separation method Methods 0.000 description 8
- 239000000047 product Substances 0.000 description 7
- 241001550224 Apha Species 0.000 description 6
- 235000013365 dairy product Nutrition 0.000 description 6
- 239000000835 fiber Substances 0.000 description 6
- 238000004519 manufacturing process Methods 0.000 description 6
- 230000002906 microbiologic effect Effects 0.000 description 6
- 238000010561 standard procedure Methods 0.000 description 6
- 108010084185 Cellulases Proteins 0.000 description 5
- 102000005575 Cellulases Human genes 0.000 description 5
- 239000005022 packaging material Substances 0.000 description 5
- 239000008188 pellet Substances 0.000 description 5
- 238000007400 DNA extraction Methods 0.000 description 4
- 238000001514 detection method Methods 0.000 description 4
- 239000002054 inoculum Substances 0.000 description 4
- 239000002609 medium Substances 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 239000000758 substrate Substances 0.000 description 4
- 101100230385 Dickeya dadantii (strain 3937) celZ gene Proteins 0.000 description 3
- 229920002488 Hemicellulose Polymers 0.000 description 3
- 230000015556 catabolic process Effects 0.000 description 3
- 230000001461 cytolytic effect Effects 0.000 description 3
- 239000002657 fibrous material Substances 0.000 description 3
- 238000000227 grinding Methods 0.000 description 3
- 238000011534 incubation Methods 0.000 description 3
- 238000011081 inoculation Methods 0.000 description 3
- 238000012544 monitoring process Methods 0.000 description 3
- 230000035945 sensitivity Effects 0.000 description 3
- 241000894007 species Species 0.000 description 3
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 2
- 108010008885 Cellulose 1,4-beta-Cellobiosidase Proteins 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- 101710121765 Endo-1,4-beta-xylanase Proteins 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
- 229920002472 Starch Polymers 0.000 description 2
- 241000223259 Trichoderma Species 0.000 description 2
- 239000011324 bead Substances 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 235000019504 cigarettes Nutrition 0.000 description 2
- 238000000576 coating method Methods 0.000 description 2
- 230000001332 colony forming effect Effects 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 230000009089 cytolysis Effects 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- PHTQWCKDNZKARW-UHFFFAOYSA-N isoamylol Chemical compound CC(C)CCO PHTQWCKDNZKARW-UHFFFAOYSA-N 0.000 description 2
- 239000007791 liquid phase Substances 0.000 description 2
- 239000011159 matrix material Substances 0.000 description 2
- 238000012856 packing Methods 0.000 description 2
- 238000009512 pharmaceutical packaging Methods 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- 238000002203 pretreatment Methods 0.000 description 2
- 235000019698 starch Nutrition 0.000 description 2
- 239000008107 starch Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 239000001974 tryptic soy broth Substances 0.000 description 2
- 108010050327 trypticase-soy broth Proteins 0.000 description 2
- 108020004465 16S ribosomal RNA Proteins 0.000 description 1
- 239000004382 Amylase Substances 0.000 description 1
- 108010065511 Amylases Proteins 0.000 description 1
- 102000013142 Amylases Human genes 0.000 description 1
- 241000006378 Bacillus cereus group Species 0.000 description 1
- 102100032487 Beta-mannosidase Human genes 0.000 description 1
- 229920002134 Carboxymethyl cellulose Polymers 0.000 description 1
- 229920003043 Cellulose fiber Polymers 0.000 description 1
- 238000007399 DNA isolation Methods 0.000 description 1
- 108010059820 Polygalacturonase Proteins 0.000 description 1
- 229920001131 Pulp (paper) Polymers 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 239000007984 Tris EDTA buffer Substances 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 235000019418 amylase Nutrition 0.000 description 1
- 230000003698 anagen phase Effects 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 239000008346 aqueous phase Substances 0.000 description 1
- 108010055059 beta-Mannosidase Proteins 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 229910000019 calcium carbonate Inorganic materials 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- ZYWFEOZQIUMEGL-UHFFFAOYSA-N chloroform;3-methylbutan-1-ol;phenol Chemical compound ClC(Cl)Cl.CC(C)CCO.OC1=CC=CC=C1 ZYWFEOZQIUMEGL-UHFFFAOYSA-N 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000012364 cultivation method Methods 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 230000014670 detection of bacterium Effects 0.000 description 1
- 230000001627 detrimental effect Effects 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 108010091371 endoglucanase 1 Proteins 0.000 description 1
- 108010091384 endoglucanase 2 Proteins 0.000 description 1
- 108010092450 endoglucanase Z Proteins 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 108010093305 exopolygalacturonase Proteins 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 235000013410 fast food Nutrition 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 239000005003 food packaging material Substances 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 230000002573 hemicellulolytic effect Effects 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 238000005360 mashing Methods 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 238000013048 microbiological method Methods 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 238000004537 pulping Methods 0.000 description 1
- 238000004451 qualitative analysis Methods 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 238000013207 serial dilution Methods 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 230000035899 viability Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/22—Testing for sterility conditions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- D—TEXTILES; PAPER
- D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
- D21C—PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCES FROM CELLULOSE-CONTAINING MATERIALS; REGENERATION OF PULPING LIQUORS; APPARATUS THEREFOR
- D21C5/00—Other processes for obtaining cellulose, e.g. cooking cotton linters ; Processes characterised by the choice of cellulose-containing starting materials
- D21C5/005—Treatment of cellulose-containing material with microorganisms or enzymes
-
- D—TEXTILES; PAPER
- D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
- D21H—PULP COMPOSITIONS; PREPARATION THEREOF NOT COVERED BY SUBCLASSES D21C OR D21D; IMPREGNATING OR COATING OF PAPER; TREATMENT OF FINISHED PAPER NOT COVERED BY CLASS B31 OR SUBCLASS D21G; PAPER NOT OTHERWISE PROVIDED FOR
- D21H17/00—Non-fibrous material added to the pulp, characterised by its constitution; Paper-impregnating material characterised by its constitution
- D21H17/005—Microorganisms or enzymes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/34—Paper
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/34—Paper
- G01N33/343—Paper pulp
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/50—Other enzymatic activities
- C12Q2521/531—Glycosylase
-
- D—TEXTILES; PAPER
- D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
- D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
- D06M16/00—Biochemical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. enzymatic
- D06M16/003—Biochemical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. enzymatic with enzymes or microorganisms
-
- D—TEXTILES; PAPER
- D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
- D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
- D06M2101/00—Chemical constitution of the fibres, threads, yarns, fabrics or fibrous goods made from such materials, to be treated
- D06M2101/02—Natural fibres, other than mineral fibres
- D06M2101/04—Vegetal fibres
- D06M2101/06—Vegetal fibres cellulosic
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/924—Hydrolases (3) acting on glycosyl compounds (3.2)
- G01N2333/942—Hydrolases (3) acting on glycosyl compounds (3.2) acting on beta-1, 4-glucosidic bonds, e.g. cellulase
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Wood Science & Technology (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Epidemiology (AREA)
- Public Health (AREA)
- Toxicology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SE1451333A SE538958C2 (en) | 2014-11-07 | 2014-11-07 | Improved method for determination of microorganisms |
| PCT/IB2015/058278 WO2016071805A1 (en) | 2014-11-07 | 2015-10-27 | Improved method for determination of microorganisms |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2798262T3 true ES2798262T3 (es) | 2020-12-10 |
Family
ID=55908666
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES15857723T Active ES2798262T3 (es) | 2014-11-07 | 2015-10-27 | Método mejorado para la determinación de microorganismos |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US10017806B2 (https=) |
| EP (1) | EP3215632B1 (https=) |
| JP (1) | JP6769957B2 (https=) |
| CN (1) | CN107027314B (https=) |
| AU (1) | AU2015341444B2 (https=) |
| BR (1) | BR112017009616B1 (https=) |
| ES (1) | ES2798262T3 (https=) |
| SE (1) | SE538958C2 (https=) |
| UA (1) | UA121557C2 (https=) |
| WO (1) | WO2016071805A1 (https=) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3476928A1 (en) * | 2017-10-27 | 2019-05-01 | Envall Consulting AB | Detection of bacterial growth |
| CN111556967B (zh) * | 2017-12-20 | 2023-04-04 | 尤妮佳股份有限公司 | 再循环浆粕纤维的制造方法 |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3542367B2 (ja) * | 1993-11-15 | 2004-07-14 | キヤノン株式会社 | 微生物の分離精製回収方法、微生物個体数の計測方法および微生物の核酸回収方法 |
| FI101809B (fi) * | 1996-06-11 | 1998-08-31 | Valtion Teknillinen | Menetelmä näytteen analysoimiseksi hiilihydraattimatriisista |
| US20040014122A1 (en) * | 1998-05-27 | 2004-01-22 | Breen Alexander W. | Detection of spore forming bacteria |
| US5928875A (en) * | 1998-05-27 | 1999-07-27 | Betzdearborn Inc. | Primers for the detection of spore forming bacteria |
| JP4207419B2 (ja) * | 2001-11-29 | 2009-01-14 | 栗田工業株式会社 | 製紙工程における製品の付着物の付着原因発生場所の探索方法および微生物制御方法 |
| WO2004042082A1 (en) * | 2002-11-06 | 2004-05-21 | Kemira Oyj | A method for monitoring the presence of harmful microorganisms in paper industry |
| JP4788150B2 (ja) * | 2005-02-10 | 2011-10-05 | 栗田工業株式会社 | 製紙工程における付着物の分析方法 |
| FI119434B (fi) * | 2006-04-13 | 2008-11-14 | Ab Enzymes Oy | Entsyymifuusioproteiineja ja niiden käyttö |
| WO2010129727A1 (en) * | 2009-05-06 | 2010-11-11 | 3M Innovative Properties Company | Coated substrates comprising a cell extractant and biodetection methods thereof |
| JP5692489B2 (ja) * | 2010-01-15 | 2015-04-01 | 東洋製罐グループホールディングス株式会社 | 微生物の検出方法 |
| US9295745B2 (en) * | 2012-01-24 | 2016-03-29 | Nalco Company | Method for constructing a diversity index and a viability index of microorganisms in process samples |
| TWI582238B (zh) * | 2012-07-17 | 2017-05-11 | 奈寇公司 | 建構於處理樣品中微生物的多樣性指數及生存力指數之方法 |
-
2014
- 2014-11-07 SE SE1451333A patent/SE538958C2/en unknown
-
2015
- 2015-10-27 AU AU2015341444A patent/AU2015341444B2/en active Active
- 2015-10-27 CN CN201580063755.9A patent/CN107027314B/zh active Active
- 2015-10-27 US US15/524,718 patent/US10017806B2/en active Active
- 2015-10-27 ES ES15857723T patent/ES2798262T3/es active Active
- 2015-10-27 BR BR112017009616-1A patent/BR112017009616B1/pt active IP Right Grant
- 2015-10-27 EP EP15857723.9A patent/EP3215632B1/en active Active
- 2015-10-27 WO PCT/IB2015/058278 patent/WO2016071805A1/en not_active Ceased
- 2015-10-27 JP JP2017524000A patent/JP6769957B2/ja active Active
- 2015-10-27 UA UAA201705499A patent/UA121557C2/uk unknown
Also Published As
| Publication number | Publication date |
|---|---|
| JP2017532977A (ja) | 2017-11-09 |
| EP3215632B1 (en) | 2020-05-06 |
| US10017806B2 (en) | 2018-07-10 |
| US20170321243A1 (en) | 2017-11-09 |
| SE1451333A1 (en) | 2016-05-23 |
| JP6769957B2 (ja) | 2020-10-14 |
| UA121557C2 (uk) | 2020-06-25 |
| AU2015341444A1 (en) | 2017-05-04 |
| CN107027314B (zh) | 2021-02-19 |
| CA2966556A1 (en) | 2016-05-12 |
| WO2016071805A1 (en) | 2016-05-12 |
| BR112017009616A2 (pt) | 2017-12-19 |
| SE538958C2 (en) | 2017-03-07 |
| BR112017009616B1 (pt) | 2023-02-14 |
| CN107027314A (zh) | 2017-08-08 |
| AU2015341444B2 (en) | 2021-10-14 |
| EP3215632A4 (en) | 2018-06-20 |
| EP3215632A1 (en) | 2017-09-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Adiguzel et al. | A novel endo-β-1, 4-xylanase from Pediococcus acidilactici GC25; purification, characterization and application in clarification of fruit juices | |
| US20240165015A1 (en) | Lactobacillus rhamnosus, ferment lysate for regulating skin microecology, preparation method, and application | |
| Choudhary et al. | Screening of alkaline protease production by fungal isolates from different habitats of Sagar and Jabalpur district (MP) | |
| Nursyirwani et al. | Isolation of cellulolytic bacteria from mangrove sediment in Dumai marine station riau and the antibacterial activity against pathogens | |
| Fernández-Pacheco et al. | Study of potential probiotic and biotechnological properties of non-Saccharomyces yeasts from fruit Brazilian ecosystems | |
| ES2798262T3 (es) | Método mejorado para la determinación de microorganismos | |
| Tsotetsi et al. | Isolation and identification of xylanase producing thermophilic bacteria from compost piles and optimization of xylanase production. | |
| Hardiansyah et al. | Identification of plant growth promoting rhizobacteria from thorny bamboo rhizosphere with 3% KOH gram test and gram staining test | |
| Emochone et al. | Isolation, partial purification and characterization of proteases from Aspergillus niger under solid-state fermentation | |
| Ajayi et al. | Studies on pectinolytic and proteolytic enzymes from deteriorated grapes (Vitis vinifera) | |
| Okonkwo | Effect of metal ions and enzyme inhibitor on the activity of cellulase enzyme of aspergillus flavus | |
| Shaibu et al. | Production and extraction of pectinase from Penicillum citrinum using tomatoes peel as carbon source | |
| Garg et al. | Production of alkaline xylanase by an alkalo-thermophilic bacteria, Bacillus halodurans, MTCC 9512 isolated from dung | |
| CA2966556C (en) | Improved method for determination of microorganisms | |
| Verma et al. | Qualitative estimation of amylase enzyme activity of fungal species isolated from iron ore mined overburden soil | |
| Kafilzadeh et al. | Isolation of amylase producing aquatic Actinomycetes from the sediments of mangrove forests in south of Iran | |
| Sani et al. | Production and screening of Streptomyces-extracellular chitinase | |
| Díaz et al. | Production of hydrolytic enzymes from grape pomace and orange peels mixed substrate fermentation by Aspergillus awamori | |
| CN116590179A (zh) | 迟缓乳杆菌、含有该菌的发酵剂以及它们的应用 | |
| Shakoori et al. | Screening, optimization and characterization of xylanase by locally isolated bacteria | |
| Soeka et al. | Production and characterization of cellulases derived from saprophytic fungi Penicillium bilaiae InaCC F16 | |
| Srivastav et al. | Starch hydrolyzing enzyme production from fruit peels using Aspergillus niger | |
| Ghoshal et al. | Isolation, screening and optimization of xylanase production in submerged fermentation using P. citrinum | |
| Mahmoud et al. | Antagonistic activities of potato associated bacteria via their production of hydrolytic enzymes with special reference to pectinases | |
| Kurniawan et al. | The Potential of Endophytic Bacteria in Controlling Cylindrocladium sp., The Cause of Leaf Blight on Eucalyptus pellita Seedlings by In-Vitro |