ES2784357T3 - Composiciones y procedimientos para la detección de Mycobacterium tuberculosis - Google Patents

Composiciones y procedimientos para la detección de Mycobacterium tuberculosis Download PDF

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Publication number
ES2784357T3
ES2784357T3 ES16750429T ES16750429T ES2784357T3 ES 2784357 T3 ES2784357 T3 ES 2784357T3 ES 16750429 T ES16750429 T ES 16750429T ES 16750429 T ES16750429 T ES 16750429T ES 2784357 T3 ES2784357 T3 ES 2784357T3
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ES
Spain
Prior art keywords
sample
mtb
amplification
amplification product
esxj
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES16750429T
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English (en)
Inventor
Jenny A Johnson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
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F Hoffmann La Roche AG
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Publication date
Application filed by F Hoffmann La Roche AG filed Critical F Hoffmann La Roche AG
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Publication of ES2784357T3 publication Critical patent/ES2784357T3/es
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Un procedimiento para detectar Mycobacterium tuberculosis (MTB) y otros miembros del complejo de MTB en una muestra, comprendiendo el procedimiento: - llevar a cabo una etapa de amplificación que comprende poner en contacto la muestra con un conjunto de cebadores paraesxJ para producir un producto de amplificación si un ácido nucleico deesxJ está presente en la muestra; - realizar una etapa de hibridación que comprende poner en contacto el producto de amplificación con una o más sondas para esxJ detectables; y - detectar la presencia o ausencia del producto de amplificación, en el que la presencia del producto de amplificación es indicativa de la presencia de MTB en la muestra y en el que la ausencia del producto de amplificación es indicativa de la ausencia de MTB en la muestra; en el que el conjunto de cebadores para esxJ comprende un primer cebador que comprende una primera secuencia de oligonucleótidos seleccionada del grupo que consiste en SEQ ID NO: 1, 7 y 9 o un complemento de las mismas, y un segundo cebador que comprende una segunda secuencia de oligonucleótidos seleccionada del grupo que consiste en SEQ ID NO: 15-17, o un complemento de las mismas, y en el que la sonda para esxJ detectable comprende una tercera secuencia de oligonucleótidos SEQ ID NO: 30, o el complemento de la misma.
ES16750429T 2015-08-06 2016-08-04 Composiciones y procedimientos para la detección de Mycobacterium tuberculosis Active ES2784357T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US14/819,427 US9970062B2 (en) 2015-08-06 2015-08-06 Compositions and methods for detection of mycobacterium tuberculosis
PCT/EP2016/068608 WO2017021478A1 (en) 2015-08-06 2016-08-04 Compositions and methods for detection of mycobacterium tuberculosis

Publications (1)

Publication Number Publication Date
ES2784357T3 true ES2784357T3 (es) 2020-09-24

Family

ID=56682107

Family Applications (1)

Application Number Title Priority Date Filing Date
ES16750429T Active ES2784357T3 (es) 2015-08-06 2016-08-04 Composiciones y procedimientos para la detección de Mycobacterium tuberculosis

Country Status (7)

Country Link
US (2) US9970062B2 (es)
EP (1) EP3332026B1 (es)
JP (1) JP6867369B2 (es)
CN (1) CN107922980B (es)
CA (1) CA2994606C (es)
ES (1) ES2784357T3 (es)
WO (1) WO2017021478A1 (es)

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0866071B1 (en) * 1997-03-20 2004-10-20 F. Hoffmann-La Roche Ag Modified primers
US20040018574A1 (en) * 1998-08-25 2004-01-29 Marcel Behr Molecular differences between species of the M. tuberculosis complex
CN1995380B (zh) * 2006-01-05 2010-05-12 中国人民解放军总医院第二附属医院 一种检测和鉴定分枝杆菌菌种的方法及其专用试剂盒
EP2397854B1 (en) * 2006-03-14 2014-06-04 Oregon Health and Science University Methods for detecting a mycobacterium tuberculosis infection
EP2385371B1 (en) * 2008-09-22 2014-10-22 Oregon Health and Science University Methods for detecting a mycobacterium tuberculosis infection

Also Published As

Publication number Publication date
EP3332026B1 (en) 2020-02-05
JP2018527914A (ja) 2018-09-27
US20190323063A1 (en) 2019-10-24
EP3332026A1 (en) 2018-06-13
US9970062B2 (en) 2018-05-15
WO2017021478A1 (en) 2017-02-09
JP6867369B2 (ja) 2021-04-28
CN107922980B (zh) 2021-11-16
US11028451B2 (en) 2021-06-08
CA2994606A1 (en) 2017-02-09
CN107922980A (zh) 2018-04-17
CA2994606C (en) 2022-06-21
US20170037452A1 (en) 2017-02-09

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