MX2021002166A - Metodos de deteccion basados en acido nucleico. - Google Patents

Metodos de deteccion basados en acido nucleico.

Info

Publication number
MX2021002166A
MX2021002166A MX2021002166A MX2021002166A MX2021002166A MX 2021002166 A MX2021002166 A MX 2021002166A MX 2021002166 A MX2021002166 A MX 2021002166A MX 2021002166 A MX2021002166 A MX 2021002166A MX 2021002166 A MX2021002166 A MX 2021002166A
Authority
MX
Mexico
Prior art keywords
nucleic acid
acid molecule
detection methods
acid based
based detection
Prior art date
Application number
MX2021002166A
Other languages
English (en)
Inventor
Arron Craig Tolley
David Harry John Bunka
Original Assignee
Aptamer Diagnostics Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Aptamer Diagnostics Ltd filed Critical Aptamer Diagnostics Ltd
Publication of MX2021002166A publication Critical patent/MX2021002166A/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5023—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures with a sample being transported to, and subsequently stored in an absorbent for analysis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0663—Stretching or orienting elongated molecules or particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0636—Integrated biosensor, microarrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0663—Whole sensors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/069—Absorbents; Gels to retain a fluid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0825—Test strips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/12—Specific details about materials
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Hematology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Fluid Mechanics (AREA)
  • Cell Biology (AREA)
  • Dispersion Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Las modalidades de la presente invención se refieren a un aparato y métodos para detectar y/o cuantificar un resto objetivo en una muestra que comprenden el uso de un evento de recaptura basado en una molécula de ácido nucleico; particularmente, aunque no exclusivamente, ciertas modalidades de la presente invención se refieren a aparatos y ensayos que comprenden un desplazamiento de una molécula de ácido nucleico inmovilizada para formar un complejo de objetivo-molécula de ácido nucleico y la detección de un evento de recaptura posterior del complejo de objetivo-ácido nucleico objetivo o la molécula de ácido nucleico por sí sola; otros aspectos y modalidades se describen en la presente.
MX2021002166A 2018-08-23 2019-08-22 Metodos de deteccion basados en acido nucleico. MX2021002166A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB1813789.3A GB201813789D0 (en) 2018-08-23 2018-08-23 Nucleic acid based detection methods
PCT/GB2019/052361 WO2020039201A1 (en) 2018-08-23 2019-08-22 Nucleic acid based detection methods

Publications (1)

Publication Number Publication Date
MX2021002166A true MX2021002166A (es) 2021-04-28

Family

ID=63715252

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2021002166A MX2021002166A (es) 2018-08-23 2019-08-22 Metodos de deteccion basados en acido nucleico.

Country Status (13)

Country Link
US (1) US20220119883A1 (es)
EP (1) EP3841218B8 (es)
JP (1) JP7503320B2 (es)
KR (1) KR20210046747A (es)
CN (1) CN113056564A (es)
AU (1) AU2019323754A1 (es)
BR (1) BR112021003226A2 (es)
CA (1) CA3110399A1 (es)
GB (1) GB201813789D0 (es)
MX (1) MX2021002166A (es)
SG (1) SG11202101573SA (es)
WO (1) WO2020039201A1 (es)
ZA (1) ZA202101530B (es)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201819580D0 (en) * 2018-11-30 2019-01-16 Aptamer Diagnostics Ltd Aptamers against IMATINIB
EP4080210A1 (en) * 2021-04-20 2022-10-26 Denso Corporation Detection method
EP4080219A1 (en) * 2021-04-20 2022-10-26 Denso Corporation Conjugate
KR102703684B1 (ko) * 2021-07-13 2024-09-04 재단법인 바이오나노헬스가드연구단 미생물 현장 오염 검출용 조성물 및 이의 용도
CN114437709B (zh) * 2021-09-15 2023-07-14 中国科学院海洋研究所 一种核酸功能化mof材料及其制备和应用
WO2024191806A1 (en) * 2023-03-10 2024-09-19 Illumina, Inc. Aptamer detection techniques

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6242246B1 (en) * 1997-12-15 2001-06-05 Somalogic, Inc. Nucleic acid ligand diagnostic Biochip
DE602004032125D1 (de) 2003-05-07 2011-05-19 Coris Bioconcept Sprl Einstufige oligochromatographische vorrichtung und verfahren zu ihrer verwendung
DE10336809B4 (de) 2003-08-07 2007-08-02 Charité - Universitätsmedizin Berlin EKG-System zur grossflächigen Messung von EKG-Signalen
US7799554B2 (en) * 2006-03-16 2010-09-21 The Board Of Trustees Of The University Of Illinois Lateral flow devices
EP2083269B1 (en) 2006-09-27 2013-09-04 National University Corporation Tokyo University of Agriculture and Technology Method of assaying target substance in sample, aptamer molecule and method of constructing the same
EP2616557B1 (en) * 2010-09-16 2014-12-24 Australian Centre for Plant Functional Genomics Pty Ltd. Capture based nucleic acid detection
WO2012172546A1 (en) * 2011-06-15 2012-12-20 Zak Naomi B Target-recognition compositions comprising novel synthetic conjugates for trapping and diagnosis of a target molecule
WO2013147217A1 (ja) 2012-03-30 2013-10-03 国立大学法人九州大学 センサ、検出方法、検出システム、及び、検出装置

Also Published As

Publication number Publication date
WO2020039201A1 (en) 2020-02-27
CN113056564A (zh) 2021-06-29
EP3841218B8 (en) 2024-08-07
JP7503320B2 (ja) 2024-06-20
SG11202101573SA (en) 2021-03-30
JP2021535409A (ja) 2021-12-16
GB201813789D0 (en) 2018-10-10
AU2019323754A1 (en) 2021-04-08
EP3841218B1 (en) 2024-06-26
US20220119883A1 (en) 2022-04-21
ZA202101530B (en) 2022-10-26
EP3841218C0 (en) 2024-06-26
CA3110399A1 (en) 2020-02-27
KR20210046747A (ko) 2021-04-28
BR112021003226A2 (pt) 2021-05-18
EP3841218A1 (en) 2021-06-30

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