ES2776157T3 - Citómetro de clasificación de flujo, método y medios legibles por computadora - Google Patents
Citómetro de clasificación de flujo, método y medios legibles por computadora Download PDFInfo
- Publication number
- ES2776157T3 ES2776157T3 ES13706356T ES13706356T ES2776157T3 ES 2776157 T3 ES2776157 T3 ES 2776157T3 ES 13706356 T ES13706356 T ES 13706356T ES 13706356 T ES13706356 T ES 13706356T ES 2776157 T3 ES2776157 T3 ES 2776157T3
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- Spain
- Prior art keywords
- drop
- drops
- fluid stream
- unwanted
- droplet
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000000034 method Methods 0.000 title claims abstract description 49
- 239000012530 fluid Substances 0.000 claims abstract description 127
- 238000000926 separation method Methods 0.000 claims abstract description 7
- 239000002245 particle Substances 0.000 claims description 94
- 238000012545 processing Methods 0.000 claims description 14
- 230000007935 neutral effect Effects 0.000 claims description 5
- 230000015654 memory Effects 0.000 description 20
- 238000010586 diagram Methods 0.000 description 14
- 238000004891 communication Methods 0.000 description 9
- 230000006870 function Effects 0.000 description 8
- 230000015572 biosynthetic process Effects 0.000 description 6
- 238000005286 illumination Methods 0.000 description 4
- 230000008859 change Effects 0.000 description 3
- 238000012512 characterization method Methods 0.000 description 3
- 238000011109 contamination Methods 0.000 description 3
- 238000006073 displacement reaction Methods 0.000 description 3
- 230000003287 optical effect Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 2
- 230000004927 fusion Effects 0.000 description 2
- 238000003384 imaging method Methods 0.000 description 2
- 230000002093 peripheral effect Effects 0.000 description 2
- 201000005569 Gout Diseases 0.000 description 1
- 230000005856 abnormality Effects 0.000 description 1
- 206010000210 abortion Diseases 0.000 description 1
- 230000003466 anti-cipated effect Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 238000000149 argon plasma sintering Methods 0.000 description 1
- 230000000712 assembly Effects 0.000 description 1
- 238000000429 assembly Methods 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 230000009849 deactivation Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000005684 electric field Effects 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000004973 liquid crystal related substance Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 230000001052 transient effect Effects 0.000 description 1
- 230000007723 transport mechanism Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1425—Optical investigation techniques, e.g. flow cytometry using an analyser being characterised by its control arrangement
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1404—Handling flow, e.g. hydrodynamic focusing
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/149—Optical investigation techniques, e.g. flow cytometry specially adapted for sorting particles, e.g. by their size or optical properties
- G01N15/1492—Optical investigation techniques, e.g. flow cytometry specially adapted for sorting particles, e.g. by their size or optical properties within droplets
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/08—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/149—Optical investigation techniques, e.g. flow cytometry specially adapted for sorting particles, e.g. by their size or optical properties
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1021—Measuring mass of individual particles
Landscapes
- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Dispersion Chemistry (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Electrostatic Separation (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261597022P | 2012-02-09 | 2012-02-09 | |
| PCT/US2013/025308 WO2013119924A1 (en) | 2012-02-09 | 2013-02-08 | Sorting flow cytometer |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2776157T3 true ES2776157T3 (es) | 2020-07-29 |
Family
ID=47750827
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES13706356T Active ES2776157T3 (es) | 2012-02-09 | 2013-02-08 | Citómetro de clasificación de flujo, método y medios legibles por computadora |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US10190959B2 (2) |
| EP (1) | EP2812671B1 (2) |
| JP (1) | JP6192665B2 (2) |
| CN (1) | CN104204768B (2) |
| AU (1) | AU2013216878B2 (2) |
| ES (1) | ES2776157T3 (2) |
| IN (1) | IN2014DN06692A (2) |
| SG (1) | SG11201404780VA (2) |
| WO (1) | WO2013119924A1 (2) |
Families Citing this family (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10481069B2 (en) * | 2011-01-03 | 2019-11-19 | Cytonome/St, Llc | Method and apparatus for monitoring and optimizing microfluidic particle sorting |
| JP5973471B2 (ja) * | 2011-03-03 | 2016-08-23 | ライフ テクノロジーズ コーポレーション | サンプリングプローブ、システム、装置、および方法 |
| WO2015056516A1 (ja) * | 2013-10-16 | 2015-04-23 | ソニー株式会社 | 粒子分取装置、粒子分取方法及びプログラム |
| JP6136843B2 (ja) * | 2013-10-17 | 2017-05-31 | ソニー株式会社 | 粒子分取装置、粒子分取方法及びプログラム |
| US9709556B2 (en) | 2013-12-11 | 2017-07-18 | Analiza, Inc. | Devices and methods for determining and/or isolating circulating cancer cells |
| US20150160246A1 (en) | 2013-12-11 | 2015-06-11 | Analiza, Inc. | Devices and methods for determining and/or isolating cells such as circulating cancer or fetal cells |
| USD787701S1 (en) * | 2014-03-21 | 2017-05-23 | Intellicyt Corporation | Flow cytometer system shelf unit |
| JP2016080563A (ja) * | 2014-10-20 | 2016-05-16 | 日本光電工業株式会社 | 分析システム、分析装置 |
| US10091279B2 (en) | 2015-05-27 | 2018-10-02 | FlowJo, LLC | Wireless connected laboratory |
| US11254557B2 (en) | 2016-05-17 | 2022-02-22 | Sony Corporation | Particle extraction apparatus and particle extraction method |
| FI3469338T3 (fi) * | 2016-06-10 | 2025-05-06 | Univ California | Kuvapohjaisia solunlajittelujärjestelmiä ja -menetelmiä |
| WO2017222461A1 (en) * | 2016-06-21 | 2017-12-28 | Giatrellis Sarantis | System and method for precision deposition of liquid droplets |
| CN110945139B (zh) | 2017-05-18 | 2023-09-05 | 10X基因组学有限公司 | 用于分选液滴和珠的方法和系统 |
| US10544413B2 (en) | 2017-05-18 | 2020-01-28 | 10X Genomics, Inc. | Methods and systems for sorting droplets and beads |
| US20190064173A1 (en) | 2017-08-22 | 2019-02-28 | 10X Genomics, Inc. | Methods of producing droplets including a particle and an analyte |
| WO2019083852A1 (en) | 2017-10-26 | 2019-05-02 | 10X Genomics, Inc. | MICROFLUIDIC CHANNEL NETWORKS FOR PARTITIONING |
| JP7366931B2 (ja) * | 2018-04-27 | 2023-10-23 | ベクトン・ディキンソン・アンド・カンパニー | 制御されたエアロゾル含有量を有する密閉された液滴ソータを有するフローサイトメータおよびそれを使用する方法 |
| CN113302470B (zh) * | 2019-02-08 | 2024-11-08 | 贝克顿·迪金森公司 | 液滴分选决策模块、系统及其使用方法 |
| CN109916803A (zh) * | 2019-02-27 | 2019-06-21 | 苏州朗如精密机械科技有限公司 | 一种流式细胞分析仪样本分选检测模块及其全自动分选检测方法 |
| EP3956649A4 (en) | 2019-04-19 | 2023-01-04 | Becton, Dickinson and Company | UNDERSAMPLING FLOW CYTOMETRIC EVENT DATA |
| CN112730408B (zh) * | 2020-12-24 | 2024-07-09 | 贝克曼库尔特生物科技（苏州）有限公司 | 液流检测系统和液流检测方法以及分选装置 |
| CN115824736B (zh) * | 2022-11-10 | 2023-10-20 | 东西分析仪器(天津)有限公司 | 一种微量样品点样头 |
| CN115962995B (zh) * | 2022-12-26 | 2023-07-04 | 中国科学院基础医学与肿瘤研究所(筹) | 一种全自动微生物染色制片装置 |
| CN118681609B (zh) * | 2023-03-24 | 2026-02-13 | 深圳华大生命科学研究院 | 液滴微流控分选方法、分选系统、电子设备及存储介质 |
Family Cites Families (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4347935A (en) * | 1979-05-16 | 1982-09-07 | The United States Of America As Represented By The United States Department Of Energy | Method and apparatus for electrostatically sorting biological cells |
| JPS62167478A (ja) * | 1985-11-29 | 1987-07-23 | Shimadzu Corp | 粒子分取装置 |
| US4981580A (en) * | 1989-05-01 | 1991-01-01 | Coulter Corporation | Coincidence arbitration in a flow cytomery sorting system |
| US5199576A (en) * | 1991-04-05 | 1993-04-06 | University Of Rochester | System for flexibly sorting particles |
| US5483469A (en) * | 1993-08-02 | 1996-01-09 | The Regents Of The University Of California | Multiple sort flow cytometer |
| US5617911A (en) * | 1995-09-08 | 1997-04-08 | Aeroquip Corporation | Method and apparatus for creating a free-form three-dimensional article using a layer-by-layer deposition of a support material and a deposition material |
| US6281018B1 (en) | 1998-02-26 | 2001-08-28 | Coulter International Corp. | Selective purification and enrichment sorting of flow cytometer droplets based upon analysis of droplet precursor regions |
| US6211477B1 (en) * | 1998-02-26 | 2001-04-03 | Becton Dickinson And Company | Electrostatic deceleration system for flow cytometer |
| US6248590B1 (en) * | 1998-02-27 | 2001-06-19 | Cytomation, Inc. | Method and apparatus for flow cytometry |
| US6079836A (en) * | 1998-07-20 | 2000-06-27 | Coulter International Corp. | Flow cytometer droplet break-off location adjustment mechanism |
| US20030211009A1 (en) * | 2001-05-18 | 2003-11-13 | Buchanan Kris S. | Rapid multi-material sample input system |
| WO2003062796A1 (en) * | 2002-01-22 | 2003-07-31 | Dakocytomation Denmark A/S | Environmental containment system for a flow cytometer |
| US20110177547A1 (en) * | 2004-12-10 | 2011-07-21 | Arryx, Inc. | Particle Sorting Using Fluid Streams |
| US8290625B2 (en) * | 2007-04-04 | 2012-10-16 | Beckman Coulter, Inc. | Flow cytometer sorter |
| US7691636B2 (en) * | 2007-05-23 | 2010-04-06 | Beckman Coulter, Inc. | Method and apparatus for compensating for variations in particle trajectories in electrostatic sorter for flowcell cytometer |
| JP4990746B2 (ja) * | 2007-12-14 | 2012-08-01 | ベイバイオサイエンス株式会社 | 液体フローに含まれる生物学的粒子を分別する装置ならびにその方法 |
| US8140300B2 (en) * | 2008-05-15 | 2012-03-20 | Becton, Dickinson And Company | High throughput flow cytometer operation with data quality assessment and control |
| JP5446563B2 (ja) | 2009-08-06 | 2014-03-19 | ソニー株式会社 | 微小粒子分取装置、および該微小粒子分取装置を用いたフローサイトメーター |
| JP2011237201A (ja) | 2010-05-06 | 2011-11-24 | Sony Corp | 微小粒子分取装置、マイクロチップ及びマイクロチップモジュール |
| US8705031B2 (en) * | 2011-02-04 | 2014-04-22 | Cytonome/St, Llc | Particle sorting apparatus and method |
| WO2013028947A1 (en) * | 2011-08-25 | 2013-02-28 | Sony Corporation | Characterization of motion-related error in a stream of moving micro-entities |
-
2013
- 2013-02-08 WO PCT/US2013/025308 patent/WO2013119924A1/en not_active Ceased
- 2013-02-08 ES ES13706356T patent/ES2776157T3/es active Active
- 2013-02-08 CN CN201380018973.1A patent/CN104204768B/zh active Active
- 2013-02-08 AU AU2013216878A patent/AU2013216878B2/en active Active
- 2013-02-08 SG SG11201404780VA patent/SG11201404780VA/en unknown
- 2013-02-08 JP JP2014556717A patent/JP6192665B2/ja active Active
- 2013-02-08 EP EP13706356.6A patent/EP2812671B1/en active Active
- 2013-02-08 US US14/377,623 patent/US10190959B2/en active Active
- 2013-02-08 IN IN6692DEN2014 patent/IN2014DN06692A/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| EP2812671B1 (en) | 2019-12-25 |
| JP2015507204A (ja) | 2015-03-05 |
| AU2013216878A1 (en) | 2014-08-28 |
| WO2013119924A1 (en) | 2013-08-15 |
| SG11201404780VA (en) | 2014-09-26 |
| IN2014DN06692A (2) | 2015-05-22 |
| JP6192665B2 (ja) | 2017-09-06 |
| US10190959B2 (en) | 2019-01-29 |
| AU2013216878B2 (en) | 2016-09-29 |
| CN104204768A (zh) | 2014-12-10 |
| CN104204768B (zh) | 2018-07-10 |
| EP2812671A1 (en) | 2014-12-17 |
| US20150050688A1 (en) | 2015-02-19 |
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