ES2741956T3 - Aislamiento de ácidos nucleicos - Google Patents
Aislamiento de ácidos nucleicos Download PDFInfo
- Publication number
- ES2741956T3 ES2741956T3 ES14705753T ES14705753T ES2741956T3 ES 2741956 T3 ES2741956 T3 ES 2741956T3 ES 14705753 T ES14705753 T ES 14705753T ES 14705753 T ES14705753 T ES 14705753T ES 2741956 T3 ES2741956 T3 ES 2741956T3
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- Prior art keywords
- nucleic acid
- sample
- lysate
- composition
- liquefaction
- Prior art date
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Landscapes
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- Organic Chemistry (AREA)
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- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
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| EP13156609 | 2013-02-25 | ||
| PCT/EP2014/053154 WO2014128129A1 (en) | 2013-02-25 | 2014-02-18 | Isolation of nucleic acids |
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| EP4592400A3 (en) | 2012-10-17 | 2025-10-29 | 10x Genomics Sweden AB | Methods and product for optimising localised or spatial detection of gene expression in a tissue sample |
| CA2916662C (en) | 2013-06-25 | 2022-03-08 | Prognosys Biosciences, Inc. | Methods and systems for determining spatial patterns of biological targets in a sample |
| EP3901282B1 (en) | 2015-04-10 | 2023-06-28 | Spatial Transcriptomics AB | Spatially distinguished, multiplex nucleic acid analysis of biological specimens |
| ES2815684T3 (es) | 2015-06-10 | 2021-03-30 | Biocartis N V | Detección mejorada de ADN metilado |
| CN105861659A (zh) * | 2016-04-13 | 2016-08-17 | 武汉康录生物技术有限公司 | 一种用于荧光原位杂交ffpe组织切片的预处理液及预处理方法 |
| EP4234710A3 (en) | 2017-03-28 | 2023-11-01 | Phase Scientific International, Ltd. | Method for accurate diagnosis of a disease targeting biomarkers in liquid biopsy |
| SG11201909324WA (en) | 2017-06-01 | 2019-11-28 | Yin To Chiu | Phase separation behavior modifying agents for aqueous two-phase separation within porous material |
| WO2019046563A1 (en) | 2017-09-01 | 2019-03-07 | Phase Diagnostics, Inc. | METHOD AND DEVICE FOR USING AQUEOUS BIPHASE SYSTEMS (ATPS) TO IMPROVE THE DIAGNOSIS OF SEXUALLY TRANSMITTED INFECTIONS |
| US20200318153A1 (en) * | 2017-09-01 | 2020-10-08 | Phase Diagnostics, Inc. | Method and device of using aqueous two-phase systems (ATPS) for enhancing diagnostics for dental and oral diseases |
| ES2964100T3 (es) | 2017-09-18 | 2024-04-04 | Phase Scient International Ltd | Método de utilización de un sistema acuoso bifásico para el aislamiento, purificación y/o concentración de fragmentos cortos de ácidos nucleicos |
| WO2019143895A1 (en) | 2018-01-19 | 2019-07-25 | Yin To Chiu | Spontaneous nucleic acid purification and concentration in a single step |
| EP3740588A4 (en) | 2018-01-19 | 2021-10-20 | Phase Scientific International, Ltd. | METHOD OF ISOLATION AND PURIFICATION OF NUCLEIC ACIDS USING A SOLID-LIQUID PHASE SYSTEM |
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| CN115135984B (zh) * | 2019-12-23 | 2025-12-23 | 10X基因组学有限公司 | 可逆固定试剂及其使用方法 |
| EP4081656A1 (en) | 2019-12-23 | 2022-11-02 | 10X Genomics, Inc. | Compositions and methods for using fixed biological samples in partition-based assays |
| US12405264B2 (en) | 2020-01-17 | 2025-09-02 | 10X Genomics, Inc. | Electrophoretic system and method for analyte capture |
| EP4121530A1 (en) | 2020-03-20 | 2023-01-25 | Phase Scientific International, Ltd. | Compositions and methods for ribonucleic acid extraction |
| US12416603B2 (en) | 2020-05-19 | 2025-09-16 | 10X Genomics, Inc. | Electrophoresis cassettes and instrumentation |
| EP4414459B1 (en) | 2020-05-22 | 2025-09-03 | 10X Genomics, Inc. | Simultaneous spatio-temporal measurement of gene expression and cellular activity |
| US12031177B1 (en) | 2020-06-04 | 2024-07-09 | 10X Genomics, Inc. | Methods of enhancing spatial resolution of transcripts |
| WO2021252747A1 (en) | 2020-06-10 | 2021-12-16 | 1Ox Genomics, Inc. | Fluid delivery methods |
| WO2022061150A2 (en) | 2020-09-18 | 2022-03-24 | 10X Geonomics, Inc. | Sample handling apparatus and image registration methods |
| KR20230098259A (ko) | 2020-10-29 | 2023-07-03 | 비오까르띠 엔브이 | 멀티플렉스 핵산 검출을 위한 제네릭 카트리지 및 방법 |
| EP4421491A3 (en) | 2021-02-19 | 2024-11-27 | 10X Genomics, Inc. | Method of using a modular assay support device |
| US12553805B2 (en) | 2021-08-02 | 2026-02-17 | 10X Genomics, Inc. | Methods of preserving a biological sample |
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| WO2023122033A1 (en) | 2021-12-20 | 2023-06-29 | 10X Genomics, Inc. | Self-test for pathology/histology slide imaging device |
| NL2034698B1 (en) | 2023-04-26 | 2024-11-04 | Biocartis Nv | Semi-solid phase extraction of nucleic acids using a quaternary salt and cation mixture in alkaline conditions |
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| US5538870A (en) | 1994-09-21 | 1996-07-23 | Boehringer Mannheim Corporation | Method for preparing nucleic acids for analysis and kits useful therefor |
| JP4503712B2 (ja) | 1996-09-09 | 2010-07-14 | 株式会社島津製作所 | 核酸合成法 |
| US6469159B1 (en) * | 1999-04-26 | 2002-10-22 | Robert T. Belly | Methods for extracting nucleic acids from tissue samples and paraffin-embedded tissues |
| US20030134292A1 (en) | 2001-10-30 | 2003-07-17 | Farchaus Joseph W. | Thermostable DNA polymerases and methods of making same |
| CN1922331A (zh) * | 2003-10-16 | 2007-02-28 | 第三次浪潮技术公司 | 体液的直接核酸检测 |
| CN1922318A (zh) * | 2003-12-24 | 2007-02-28 | 3M创新有限公司 | 使用固相材料分离核酸的方法和试剂盒 |
| ES2689172T3 (es) | 2005-06-23 | 2018-11-08 | Biocartis Nv | Cartucho modular, sistema y procedimiento para diagnóstico médico automatizado |
| WO2007004103A1 (en) | 2005-06-30 | 2007-01-11 | Koninklijke Philips Electronics N.V. | Cartridge for automated medical diagnostics |
| KR20100015578A (ko) | 2007-03-14 | 2010-02-12 | 시에라 몰레큘러 코포레이션 | 세포 및/또는 거대분자의 보존 및/또는 안정화를 위한 조성물, 시스템 및 방법 |
| DE102008026058A1 (de) * | 2008-05-30 | 2009-12-03 | Qiagen Gmbh | Lyse, Binde- und/oder Waschreagenz verwendbar zur Isolierung und/oder Reinigung von Nukleinsäuren |
| EP2419705B1 (en) | 2009-04-14 | 2020-12-09 | Biocartis NV | Treatment of a sample with focused acoustic energy |
| EP2454378B1 (en) | 2009-07-17 | 2015-12-23 | Canon U.S. Life Sciences, Inc. | Methods and systems for dna isolation on a microfluidic device |
| CA2786569C (en) | 2010-01-29 | 2019-04-09 | Perkinelmer Health Sciences, Inc. | Sample-to-answer microfluidic cartridge |
| GB2483858A (en) | 2010-09-21 | 2012-03-28 | Univ Hull | Amplifying nucleic acids using microfluidic device to perform PRC |
| WO2012075133A1 (en) * | 2010-11-30 | 2012-06-07 | Life Technologies Corporation | Alkylene glycols and polymers and copolymers thereof for direct isolation of nucleic acid from embedded samples |
| CN102146112B (zh) * | 2011-01-25 | 2013-07-10 | 天根生化科技（北京）有限公司 | 从福尔马林固定石蜡包埋组织中提取脱氧核糖核酸的方法 |
| US9518901B2 (en) * | 2011-06-29 | 2016-12-13 | Kabushiki Kaisha Dnaform | Pretreatment method of biological sample, detection method of RNA, and pretreatment kit |
| JP6158808B2 (ja) * | 2011-08-04 | 2017-07-05 | セイジ サイエンス，インコーポレイティド | 流体を処理するためのシステム及び方法 |
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| US10196673B2 (en) | 2019-02-05 |
| CN105164259B (zh) | 2018-02-27 |
| CA2901641A1 (en) | 2014-08-28 |
| EP2958997A1 (en) | 2015-12-30 |
| JP6567427B2 (ja) | 2019-08-28 |
| JP2016507242A (ja) | 2016-03-10 |
| CA2901641C (en) | 2021-02-09 |
| CN105164259A (zh) | 2015-12-16 |
| US20160002706A1 (en) | 2016-01-07 |
| EP2958997B1 (en) | 2019-07-17 |
| EP3674406A1 (en) | 2020-07-01 |
| WO2014128129A1 (en) | 2014-08-28 |
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