ES2741956T3 - Aislamiento de ácidos nucleicos - Google Patents
Aislamiento de ácidos nucleicos Download PDFInfo
- Publication number
- ES2741956T3 ES2741956T3 ES14705753T ES14705753T ES2741956T3 ES 2741956 T3 ES2741956 T3 ES 2741956T3 ES 14705753 T ES14705753 T ES 14705753T ES 14705753 T ES14705753 T ES 14705753T ES 2741956 T3 ES2741956 T3 ES 2741956T3
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- Prior art keywords
- nucleic acid
- sample
- lysate
- composition
- liquefaction
- Prior art date
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
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| USRE50065E1 (en) | 2012-10-17 | 2024-07-30 | 10X Genomics Sweden Ab | Methods and product for optimising localised or spatial detection of gene expression in a tissue sample |
| CN105849275B (zh) | 2013-06-25 | 2020-03-17 | 普罗格诺西斯生物科学公司 | 检测样品中生物靶标的空间分布的方法和系统 |
| HK1250245A1 (zh) | 2015-04-10 | 2018-12-07 | Spatial Transcriptomics Ab | 生物样本的空间区别、多重核酸分析 |
| EP3782731A1 (en) * | 2015-06-10 | 2021-02-24 | Biocartis N.V. | Improved detection of methylated dna |
| CN105861659A (zh) * | 2016-04-13 | 2016-08-17 | 武汉康录生物技术有限公司 | 一种用于荧光原位杂交ffpe组织切片的预处理液及预处理方法 |
| CN110462400B (zh) | 2017-03-28 | 2022-06-21 | 相达生物科技国际有限公司 | 用于在液体活检中准确诊断疾病靶向生物标记的方法 |
| CN119425158A (zh) | 2017-06-01 | 2025-02-14 | 相达生物科技美国有限公司 | 用于多孔材料中双水相分离的相分离行为改性剂 |
| US11609232B2 (en) | 2017-09-01 | 2023-03-21 | Phase Diagnostics, Inc. | Method and device of using aqueous two-phase systems (ATPS) for enhancing diagnostics for sexually transmitted infections |
| US20200318153A1 (en) * | 2017-09-01 | 2020-10-08 | Phase Diagnostics, Inc. | Method and device of using aqueous two-phase systems (ATPS) for enhancing diagnostics for dental and oral diseases |
| JP7323532B2 (ja) | 2017-09-18 | 2023-08-08 | フェーズ サイエンティフィック インターナショナル リミテッド | 短い核酸断片の単離、精製及び／又は濃縮のために水性二相系を使用する方法 |
| US12043826B2 (en) | 2018-01-19 | 2024-07-23 | Phase Scientific International, Ltd. | Spontaneous nucleic acid purification and concentration in a single step |
| US11643646B2 (en) | 2018-01-19 | 2023-05-09 | Phase Scientific International, Ltd. | Method for isolating and purifying nucleic acids using a solid-liquid phase system |
| CN111868256A (zh) | 2018-01-23 | 2020-10-30 | 比奥卡尔齐斯股份有限公司 | 用于分析解离解链曲线数据的方法 |
| US12157124B2 (en) | 2019-11-06 | 2024-12-03 | 10X Genomics, Inc. | Imaging system hardware |
| EP4081656A1 (en) | 2019-12-23 | 2022-11-02 | 10X Genomics, Inc. | Compositions and methods for using fixed biological samples in partition-based assays |
| EP4081777A1 (en) * | 2019-12-23 | 2022-11-02 | 10X Genomics, Inc. | Reversible fixing reagents and methods of use thereof |
| US12405264B2 (en) | 2020-01-17 | 2025-09-02 | 10X Genomics, Inc. | Electrophoretic system and method for analyte capture |
| AU2021239220B2 (en) | 2020-03-20 | 2024-11-14 | Phase Scientific International, Ltd. | Compositions and methods for ribonucleic acid extraction |
| US12416603B2 (en) | 2020-05-19 | 2025-09-16 | 10X Genomics, Inc. | Electrophoresis cassettes and instrumentation |
| WO2021236929A1 (en) | 2020-05-22 | 2021-11-25 | 10X Genomics, Inc. | Simultaneous spatio-temporal measurement of gene expression and cellular activity |
| US12031177B1 (en) | 2020-06-04 | 2024-07-09 | 10X Genomics, Inc. | Methods of enhancing spatial resolution of transcripts |
| ES2993269T3 (en) | 2020-09-18 | 2024-12-26 | 10X Genomics Inc | Sample handling apparatus and image registration methods |
| US20240035075A1 (en) | 2020-10-29 | 2024-02-01 | Biocartis Nv | Generic cartridge and method for multiplex nucleic acid detection |
| EP4421491A3 (en) | 2021-02-19 | 2024-11-27 | 10X Genomics, Inc. | Method of using a modular assay support device |
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| EP4441711A1 (en) | 2021-12-20 | 2024-10-09 | 10X Genomics, Inc. | Self-test for pathology/histology slide imaging device |
| NL2034698B1 (en) | 2023-04-26 | 2024-11-04 | Biocartis Nv | Semi-solid phase extraction of nucleic acids using a quaternary salt and cation mixture in alkaline conditions |
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| US5538870A (en) | 1994-09-21 | 1996-07-23 | Boehringer Mannheim Corporation | Method for preparing nucleic acids for analysis and kits useful therefor |
| JP4503712B2 (ja) | 1996-09-09 | 2010-07-14 | 株式会社島津製作所 | 核酸合成法 |
| US6469159B1 (en) * | 1999-04-26 | 2002-10-22 | Robert T. Belly | Methods for extracting nucleic acids from tissue samples and paraffin-embedded tissues |
| US20030134292A1 (en) | 2001-10-30 | 2003-07-17 | Farchaus Joseph W. | Thermostable DNA polymerases and methods of making same |
| CN1922331A (zh) * | 2003-10-16 | 2007-02-28 | 第三次浪潮技术公司 | 体液的直接核酸检测 |
| CN1918291A (zh) * | 2003-12-24 | 2007-02-21 | 3M创新有限公司 | 使用微流体装置和沉降试剂分离核酸的方法和试剂盒 |
| US9568424B2 (en) | 2005-06-23 | 2017-02-14 | Biocartis Nv | Cartridge, system and method for automated medical diagnostics |
| EP1904234B1 (en) | 2005-06-30 | 2011-10-19 | Biocartis SA | Cartridge for automated medical diagnostics |
| WO2008113017A2 (en) | 2007-03-14 | 2008-09-18 | Sierra Molecular Corporation | Compositions, systems, and methods for preservation and/or stabilization of a cell and/or macromolecule |
| DE102008026058A1 (de) * | 2008-05-30 | 2009-12-03 | Qiagen Gmbh | Lyse, Binde- und/oder Waschreagenz verwendbar zur Isolierung und/oder Reinigung von Nukleinsäuren |
| WO2010118540A1 (en) | 2009-04-14 | 2010-10-21 | Biocartis Sa | Treatment of a sample with focused acoustic energy |
| JP5815518B2 (ja) | 2009-07-17 | 2015-11-17 | キヤノン ユー．エス． ライフ サイエンシズ， インコーポレイテッドＣａｎｏｎ Ｕ．Ｓ． Ｌｉｆｅ Ｓｃｉｅｎｃｅｓ， Ｉｎｃ． | マイクロ流体装置においてｄｎａを単離する方法及びシステム |
| WO2011094577A2 (en) * | 2010-01-29 | 2011-08-04 | Micronics, Inc. | Sample-to-answer microfluidic cartridge |
| GB2483858A (en) | 2010-09-21 | 2012-03-28 | Univ Hull | Amplifying nucleic acids using microfluidic device to perform PRC |
| EP2646551B1 (en) | 2010-11-30 | 2017-06-07 | Life Technologies Corporation | Alkylene glycols and polymers and copolymers thereof for direct isolation of nucleic acid from embedded samples |
| CN102146112B (zh) * | 2011-01-25 | 2013-07-10 | 天根生化科技（北京）有限公司 | 从福尔马林固定石蜡包埋组织中提取脱氧核糖核酸的方法 |
| US9518901B2 (en) | 2011-06-29 | 2016-12-13 | Kabushiki Kaisha Dnaform | Pretreatment method of biological sample, detection method of RNA, and pretreatment kit |
| WO2013020089A2 (en) | 2011-08-04 | 2013-02-07 | Sage Science, Inc. | Systems and methods for processing fluids |
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| US10196673B2 (en) | 2019-02-05 |
| EP2958997A1 (en) | 2015-12-30 |
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| WO2014128129A1 (en) | 2014-08-28 |
| EP3674406A1 (en) | 2020-07-01 |
| JP2016507242A (ja) | 2016-03-10 |
| CA2901641C (en) | 2021-02-09 |
| CN105164259A (zh) | 2015-12-16 |
| US20160002706A1 (en) | 2016-01-07 |
| CN105164259B (zh) | 2018-02-27 |
| EP2958997B1 (en) | 2019-07-17 |
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