JP6567427B2 - 核酸の単離 - Google Patents
核酸の単離 Download PDFInfo
- Publication number
- JP6567427B2 JP6567427B2 JP2015558426A JP2015558426A JP6567427B2 JP 6567427 B2 JP6567427 B2 JP 6567427B2 JP 2015558426 A JP2015558426 A JP 2015558426A JP 2015558426 A JP2015558426 A JP 2015558426A JP 6567427 B2 JP6567427 B2 JP 6567427B2
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- sample
- lysate
- composition
- ether
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP13156609.3 | 2013-02-25 | ||
| EP13156609 | 2013-02-25 | ||
| PCT/EP2014/053154 WO2014128129A1 (en) | 2013-02-25 | 2014-02-18 | Isolation of nucleic acids |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2016507242A JP2016507242A (ja) | 2016-03-10 |
| JP2016507242A5 JP2016507242A5 (OSRAM) | 2017-03-23 |
| JP6567427B2 true JP6567427B2 (ja) | 2019-08-28 |
Family
ID=47749705
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015558426A Active JP6567427B2 (ja) | 2013-02-25 | 2014-02-18 | 核酸の単離 |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US10196673B2 (OSRAM) |
| EP (2) | EP3674406A1 (OSRAM) |
| JP (1) | JP6567427B2 (OSRAM) |
| CN (1) | CN105164259B (OSRAM) |
| CA (1) | CA2901641C (OSRAM) |
| ES (1) | ES2741956T3 (OSRAM) |
| WO (1) | WO2014128129A1 (OSRAM) |
Families Citing this family (28)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB201106254D0 (en) | 2011-04-13 | 2011-05-25 | Frisen Jonas | Method and product |
| EP3901280B1 (en) | 2012-10-17 | 2025-03-12 | 10x Genomics Sweden AB | Methods and product for optimising localised or spatial detection of gene expression in a tissue sample |
| LT3013983T (lt) | 2013-06-25 | 2023-05-10 | Prognosys Biosciences, Inc. | Erdviniai koduoti biologiniai tyrimai, naudojant mikrofluidinį įrenginį |
| HK1250245A1 (zh) | 2015-04-10 | 2018-12-07 | Spatial Transcriptomics Ab | 生物样本的空间区别、多重核酸分析 |
| US10533210B2 (en) | 2015-06-10 | 2020-01-14 | Biocartis Nv | Detection of methylated DNA |
| CN105861659A (zh) * | 2016-04-13 | 2016-08-17 | 武汉康录生物技术有限公司 | 一种用于荧光原位杂交ffpe组织切片的预处理液及预处理方法 |
| CN114839374A (zh) | 2017-03-28 | 2022-08-02 | 相达生物科技国际有限公司 | 用于在液体活检中准确诊断疾病靶向生物标记的方法 |
| EP3595791B1 (en) | 2017-06-01 | 2024-05-15 | Phase Diagnostics, Inc. | Phase separation behavior modifying agent dextran for aqueous two-phase separation within porous fibreglass material |
| WO2019046563A1 (en) | 2017-09-01 | 2019-03-07 | Phase Diagnostics, Inc. | METHOD AND DEVICE FOR USING AQUEOUS BIPHASE SYSTEMS (ATPS) TO IMPROVE THE DIAGNOSIS OF SEXUALLY TRANSMITTED INFECTIONS |
| US20200318153A1 (en) * | 2017-09-01 | 2020-10-08 | Phase Diagnostics, Inc. | Method and device of using aqueous two-phase systems (ATPS) for enhancing diagnostics for dental and oral diseases |
| JP7323532B2 (ja) | 2017-09-18 | 2023-08-08 | フェーズ サイエンティフィック インターナショナル リミテッド | 短い核酸断片の単離、精製及び／又は濃縮のために水性二相系を使用する方法 |
| CN111867721A (zh) | 2018-01-19 | 2020-10-30 | 相达生物科技国际有限公司 | 使用固-液相系统分离和纯化核酸的方法 |
| US12043826B2 (en) | 2018-01-19 | 2024-07-23 | Phase Scientific International, Ltd. | Spontaneous nucleic acid purification and concentration in a single step |
| JP2021510547A (ja) | 2018-01-23 | 2021-04-30 | バイオカルティス エン フェー | 解離融解曲線データの分析方法 |
| US12157124B2 (en) | 2019-11-06 | 2024-12-03 | 10X Genomics, Inc. | Imaging system hardware |
| US12241890B2 (en) | 2019-12-23 | 2025-03-04 | 10X Genomics, Inc. | Methods for generating barcoded nucleic acid molecules using fixed cells |
| EP4081777A1 (en) * | 2019-12-23 | 2022-11-02 | 10X Genomics, Inc. | Reversible fixing reagents and methods of use thereof |
| US12405264B2 (en) | 2020-01-17 | 2025-09-02 | 10X Genomics, Inc. | Electrophoretic system and method for analyte capture |
| WO2021185336A1 (en) | 2020-03-20 | 2021-09-23 | Phase Scientific International, Ltd. | Compositions and methods for ribonucleic acid extraction |
| WO2021236625A1 (en) | 2020-05-19 | 2021-11-25 | 10X Genomics, Inc. | Electrophoresis cassettes and instrumentation |
| WO2021236929A1 (en) | 2020-05-22 | 2021-11-25 | 10X Genomics, Inc. | Simultaneous spatio-temporal measurement of gene expression and cellular activity |
| US12031177B1 (en) | 2020-06-04 | 2024-07-09 | 10X Genomics, Inc. | Methods of enhancing spatial resolution of transcripts |
| EP4491742A3 (en) | 2020-09-18 | 2025-05-21 | 10x Genomics, Inc. | Sample handling apparatus and image registration methods |
| EP3992302A1 (en) | 2020-10-29 | 2022-05-04 | Biocartis NV | Generic cartridge and method for multiplex nucleic acid detection |
| EP4294571B8 (en) | 2021-02-19 | 2024-07-10 | 10X Genomics, Inc. | Method of using a modular assay support device |
| USD1064308S1 (en) | 2021-09-17 | 2025-02-25 | 10X Genomics, Inc. | Sample handling device |
| WO2023122033A1 (en) | 2021-12-20 | 2023-06-29 | 10X Genomics, Inc. | Self-test for pathology/histology slide imaging device |
| NL2034698B1 (en) | 2023-04-26 | 2024-11-04 | Biocartis Nv | Semi-solid phase extraction of nucleic acids using a quaternary salt and cation mixture in alkaline conditions |
Family Cites Families (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5538870A (en) | 1994-09-21 | 1996-07-23 | Boehringer Mannheim Corporation | Method for preparing nucleic acids for analysis and kits useful therefor |
| JP4503712B2 (ja) * | 1996-09-09 | 2010-07-14 | 株式会社島津製作所 | 核酸合成法 |
| US6469159B1 (en) * | 1999-04-26 | 2002-10-22 | Robert T. Belly | Methods for extracting nucleic acids from tissue samples and paraffin-embedded tissues |
| US20030134292A1 (en) | 2001-10-30 | 2003-07-17 | Farchaus Joseph W. | Thermostable DNA polymerases and methods of making same |
| CN1922331A (zh) * | 2003-10-16 | 2007-02-28 | 第三次浪潮技术公司 | 体液的直接核酸检测 |
| CN1918291A (zh) * | 2003-12-24 | 2007-02-21 | 3M创新有限公司 | 使用微流体装置和沉降试剂分离核酸的方法和试剂盒 |
| CN102268367A (zh) | 2005-06-23 | 2011-12-07 | 拜欧卡蒂斯股份公司 | 用于自动医疗诊断的盒体、系统和方法 |
| WO2007004103A1 (en) | 2005-06-30 | 2007-01-11 | Koninklijke Philips Electronics N.V. | Cartridge for automated medical diagnostics |
| JP2010535013A (ja) | 2007-03-14 | 2010-11-18 | シエラ モレキュラー コーポレイション | 細胞および／または高分子の保存および／または安定化のための組成物、システムおよび方法 |
| DE102008026058A1 (de) * | 2008-05-30 | 2009-12-03 | Qiagen Gmbh | Lyse, Binde- und/oder Waschreagenz verwendbar zur Isolierung und/oder Reinigung von Nukleinsäuren |
| RU2554572C2 (ru) | 2009-04-14 | 2015-06-27 | Биокартис Нв | Обработка образца фокусированной звуковой энергией |
| EP2454378B1 (en) | 2009-07-17 | 2015-12-23 | Canon U.S. Life Sciences, Inc. | Methods and systems for dna isolation on a microfluidic device |
| KR101851117B1 (ko) * | 2010-01-29 | 2018-04-23 | 마이크로닉스 인코포레이티드. | 샘플-투-앤서 마이크로유체 카트리지 |
| GB2483858A (en) * | 2010-09-21 | 2012-03-28 | Univ Hull | Amplifying nucleic acids using microfluidic device to perform PRC |
| EP3239296A1 (en) * | 2010-11-30 | 2017-11-01 | Life Technologies Corporation | Alkylene glycols and polymers and copolymers thereof for direct isolation of nucleic acid from embedded samples |
| CN102146112B (zh) * | 2011-01-25 | 2013-07-10 | 天根生化科技（北京）有限公司 | 从福尔马林固定石蜡包埋组织中提取脱氧核糖核酸的方法 |
| WO2013002354A1 (ja) | 2011-06-29 | 2013-01-03 | 株式会社ダナフォーム | 生体試料の前処理方法、ｒｎａの検出方法及び前処理キット |
| WO2013020089A2 (en) * | 2011-08-04 | 2013-02-07 | Sage Science, Inc. | Systems and methods for processing fluids |
-
2014
- 2014-02-18 US US14/768,871 patent/US10196673B2/en active Active
- 2014-02-18 ES ES14705753T patent/ES2741956T3/es active Active
- 2014-02-18 JP JP2015558426A patent/JP6567427B2/ja active Active
- 2014-02-18 CN CN201480023809.4A patent/CN105164259B/zh active Active
- 2014-02-18 WO PCT/EP2014/053154 patent/WO2014128129A1/en not_active Ceased
- 2014-02-18 EP EP19179423.9A patent/EP3674406A1/en not_active Withdrawn
- 2014-02-18 CA CA2901641A patent/CA2901641C/en active Active
- 2014-02-18 EP EP14705753.3A patent/EP2958997B1/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| EP3674406A1 (en) | 2020-07-01 |
| JP2016507242A (ja) | 2016-03-10 |
| CN105164259B (zh) | 2018-02-27 |
| CN105164259A (zh) | 2015-12-16 |
| CA2901641A1 (en) | 2014-08-28 |
| US10196673B2 (en) | 2019-02-05 |
| WO2014128129A1 (en) | 2014-08-28 |
| EP2958997B1 (en) | 2019-07-17 |
| CA2901641C (en) | 2021-02-09 |
| US20160002706A1 (en) | 2016-01-07 |
| EP2958997A1 (en) | 2015-12-30 |
| ES2741956T3 (es) | 2020-02-12 |
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