ES2674239T8 - Medios para células madre pluripotentes carentes de alimentadores que contienen suero humano - Google Patents

Medios para células madre pluripotentes carentes de alimentadores que contienen suero humano

Info

Publication number
ES2674239T8
ES2674239T8 ES08840374T ES08840374T ES2674239T8 ES 2674239 T8 ES2674239 T8 ES 2674239T8 ES 08840374 T ES08840374 T ES 08840374T ES 08840374 T ES08840374 T ES 08840374T ES 2674239 T8 ES2674239 T8 ES 2674239T8
Authority
ES
Spain
Prior art keywords
stem cells
human serum
pluripotent stem
containing human
cells lacking
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES08840374T
Other languages
English (en)
Other versions
ES2674239T3 (es
Inventor
Allan J Robins
Thomas C Schulz
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Viacyte Inc
Original Assignee
Viacyte Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Viacyte Inc filed Critical Viacyte Inc
Application granted granted Critical
Publication of ES2674239T3 publication Critical patent/ES2674239T3/es
Publication of ES2674239T8 publication Critical patent/ES2674239T8/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0603Embryonic cells ; Embryoid bodies
    • C12N5/0606Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0679Cells of the gastro-intestinal tract
    • C12N5/068Stem cells; Progenitors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/70Undefined extracts
    • C12N2500/80Undefined extracts from animals
    • C12N2500/84Undefined extracts from animals from mammals
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/98Xeno-free medium and culture conditions
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/15Transforming growth factor beta (TGF-β)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Reproductive Health (AREA)
  • Gynecology & Obstetrics (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
ES08840374.6T 2007-10-19 2008-10-20 Medios para células madre pluripotentes carentes de alimentadores que contienen suero humano Active ES2674239T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US875057 1978-02-06
US11/875,057 US8623650B2 (en) 2007-10-19 2007-10-19 Methods and compositions for feeder-free pluripotent stem cell media containing human serum
PCT/US2008/080516 WO2009052505A1 (en) 2007-10-19 2008-10-20 Feeder-free pluripotent stem cell media containing human serum

Publications (2)

Publication Number Publication Date
ES2674239T3 ES2674239T3 (es) 2018-06-28
ES2674239T8 true ES2674239T8 (es) 2019-01-16

Family

ID=40563869

Family Applications (2)

Application Number Title Priority Date Filing Date
ES18171601T Active ES2971288T3 (es) 2007-10-19 2008-10-20 Sistema de cultivo sin alimentador
ES08840374.6T Active ES2674239T3 (es) 2007-10-19 2008-10-20 Medios para células madre pluripotentes carentes de alimentadores que contienen suero humano

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ES18171601T Active ES2971288T3 (es) 2007-10-19 2008-10-20 Sistema de cultivo sin alimentador

Country Status (10)

Country Link
US (7) US8623650B2 (es)
EP (3) EP2215214B1 (es)
CN (2) CN101903515B (es)
AU (1) AU2008311798B2 (es)
CA (1) CA2703116C (es)
DK (1) DK3375867T3 (es)
ES (2) ES2971288T3 (es)
IL (5) IL283597B2 (es)
PL (1) PL2215214T3 (es)
WO (1) WO2009052505A1 (es)

Families Citing this family (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7432104B2 (en) * 2001-08-06 2008-10-07 Bresgen Inc. Methods for the culture of human embryonic stem cells on human feeder cells
CA2576872C (en) 2004-08-13 2013-11-12 University Of Georgia Research Foundation, Inc. Compositions and methods for self-renewal and differentiation in human embryonic stem cells
EP2420565B1 (en) 2006-02-23 2017-08-30 ViaCyte, Inc. Compositions and methods useful for culturing differentiable cells
US8623650B2 (en) 2007-10-19 2014-01-07 Viacyte, Inc. Methods and compositions for feeder-free pluripotent stem cell media containing human serum
US8338170B2 (en) 2008-04-21 2012-12-25 Viacyte, Inc. Methods for purifying endoderm and pancreatic endoderm cells derived from human embryonic stem cells
US20090298178A1 (en) * 2008-06-03 2009-12-03 D Amour Kevin Allen Growth factors for production of definitive endoderm
US8008075B2 (en) 2008-11-04 2011-08-30 Viacyte, Inc. Stem cell aggregate suspension compositions and methods of differentiation thereof
US8895300B2 (en) 2008-11-04 2014-11-25 Viacyte, Inc. Scalable primate pluripotent stem cell aggregate suspension culture and differentiation thereof
ES2932850T3 (es) 2008-11-14 2023-01-27 Viacyte Inc Encapsulación de células pancreáticas derivadas de células madre pluripotentes humanas
US9109245B2 (en) 2009-04-22 2015-08-18 Viacyte, Inc. Cell compositions derived from dedifferentiated reprogrammed cells
EP4166652A1 (en) 2009-11-12 2023-04-19 Technion Research & Development Foundation Ltd. Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state
WO2012115619A1 (en) 2011-02-21 2012-08-30 Viacyte, Inc. Loading system for an encapsulation device
JOP20200043A1 (ar) 2011-05-10 2017-06-16 Amgen Inc طرق معالجة أو منع الاضطرابات المختصة بالكوليسترول
AU2013248265B2 (en) * 2012-11-08 2018-11-01 Viacyte, Inc. Scalable primate pluripotent stem cell aggregate suspension culture and differentiation thereof
CN116212115A (zh) 2013-03-07 2023-06-06 韦尔赛特公司 三维大容量细胞包封装置组件
USD720469S1 (en) 2013-03-07 2014-12-30 Viacyte, Inc. Cell encapsulation device
US8859286B2 (en) 2013-03-14 2014-10-14 Viacyte, Inc. In vitro differentiation of pluripotent stem cells to pancreatic endoderm cells (PEC) and endocrine cells
KR102232650B1 (ko) 2013-06-11 2021-03-29 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 SC-β 세포 및 조성물 그리고 그 생성 방법
US11051900B2 (en) 2014-04-16 2021-07-06 Viacyte, Inc. Tools and instruments for use with implantable encapsulation devices
CN107614678B (zh) 2014-12-18 2021-04-30 哈佛学院校长同事会 干细胞来源的β细胞的产生方法及其使用方法
ES2985135T3 (es) 2014-12-18 2024-11-04 Harvard College Procedimientos para generar células ß derivadas de células madre y usos de las mismas
WO2016100898A1 (en) 2014-12-18 2016-06-23 President And Fellows Of Harvard College Serum-free in vitro directed differentiation protocol for generating stem cell-derived b cells and uses thereof
CN118924958A (zh) 2016-11-10 2024-11-12 韦尔赛特公司 含有pdx1胰腺内胚层细胞的细胞递送装置及其方法
CN111565707A (zh) 2017-06-14 2020-08-21 森玛治疗公司 用于递送治疗物的装置和方法
US10391156B2 (en) 2017-07-12 2019-08-27 Viacyte, Inc. University donor cells and related methods
AU2018370029B2 (en) 2017-11-15 2024-11-07 Vertex Pharmaceuticals Incorporated Islet cell manufacturing compositions and methods of use
WO2020033879A1 (en) 2018-08-10 2020-02-13 Semma Therapeutics, Inc. Stem cell derived islet differentiation
US12378572B2 (en) 2018-09-07 2025-08-05 Crispr Therapeutics Ag Universal donor cells
CN110373375A (zh) * 2019-03-19 2019-10-25 杭州原生生物科技有限公司 一种制备纤维细胞作为细胞培养基的方法和用途
AU2020283056B2 (en) 2019-05-31 2023-06-08 Viacyte, Inc. A biocompatible membrane composite
AU2020282355B2 (en) 2019-05-31 2023-11-02 Viacyte, Inc. A biocompatible membrane composite
WO2020243666A1 (en) 2019-05-31 2020-12-03 W. L. Gore & Associates, Inc. A biocompatible membrane composite
CA3139292C (en) 2019-05-31 2025-06-10 Viacyte, Inc. CELL ENCAPSULATION DEVICES WITH REGULATED OXYGEN DIFFUSION DISTANCES
US20210060163A1 (en) * 2019-09-04 2021-03-04 Vcell Therapeutics, Inc. Vaccine for treatment of cancer and method of making by stress reprogramming
KR20220058579A (ko) 2019-09-05 2022-05-09 크리스퍼 테라퓨틱스 아게 보편적 공여자 세포
US11118196B2 (en) 2019-09-05 2021-09-14 Crispr Therapeutics Ag Universal donor cells
WO2022144856A1 (en) 2020-12-31 2022-07-07 Crispr Therapeutics Ag Universal donor cells

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4309418A (en) 1980-03-25 1982-01-05 Sloan-Kettering Research Institute For Cancer Anti-tumor agent from human serum and process
US5453357A (en) 1992-10-08 1995-09-26 Vanderbilt University Pluripotential embryonic stem cells and methods of making same
US5843780A (en) 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
JP2001517927A (ja) 1996-02-28 2001-10-09 バンダービルト ユニバーシティ 胚幹細胞を作製する組成物および方法
US6331406B1 (en) 1997-03-31 2001-12-18 The John Hopkins University School Of Medicine Human enbryonic germ cell and methods of use
CA2307807C (en) 1997-10-23 2008-09-02 Andrea G. Bodnar Methods and materials for the growth of primate-derived primordial stem cells in feeder-free culture
NZ507103A (en) 1998-04-09 2002-10-25 Bresagen Ltd Cell differentiation/proliferation and maintenance factor and uses thereof
JP2002529070A (ja) 1998-11-09 2002-09-10 モナシュ・ユニヴァーシティ 胚性幹細胞
GB2396623B (en) * 2001-09-28 2006-04-05 Es Cell Int Pte Ltd Methods of derivation and propagation of undifferentiated human embryonic stem (hes) cells on feeder-free matrices and human feeder layers
HUE028026T2 (en) * 2002-12-16 2016-11-28 Technion Res & Dev Foundation Breeding system free of native cells, free of foreign matter for human embryonic stem cells
US8647873B2 (en) 2004-04-27 2014-02-11 Viacyte, Inc. PDX1 expressing endoderm
US7625753B2 (en) 2003-12-23 2009-12-01 Cythera, Inc. Expansion of definitive endoderm cells
WO2005065354A2 (en) 2003-12-31 2005-07-21 The Burnham Institute Defined media for pluripotent stem cell culture
CN101048495A (zh) * 2004-04-27 2007-10-03 赛瑟拉公司 表达pdx1的内胚层
CN101023163A (zh) * 2004-07-09 2007-08-22 赛瑟拉公司 鉴定用于分化定型内胚层的因子的方法
US8597947B2 (en) * 2004-12-29 2013-12-03 Hadasit Medical Research Services & Development Limited Undifferentiated stem cell culture systems
US20070122392A1 (en) 2005-06-22 2007-05-31 Sharon Gerecht-Nir Propagation of undifferentiated embryonic stem cells in hyaluronic acid hydrogel
EP2420565B1 (en) * 2006-02-23 2017-08-30 ViaCyte, Inc. Compositions and methods useful for culturing differentiable cells
US7964402B2 (en) * 2006-05-25 2011-06-21 Sanford-Burnham Medical Research Institute Methods for culture and production of single cell populations of human embryonic stem cells
DK2634242T3 (en) * 2006-07-14 2017-05-22 Patheon Holdings I B V IMPROVED PROCEDURE FOR CULTURING CELLS
US8623650B2 (en) 2007-10-19 2014-01-07 Viacyte, Inc. Methods and compositions for feeder-free pluripotent stem cell media containing human serum

Also Published As

Publication number Publication date
IL205115A0 (en) 2010-12-30
EP3375867B1 (en) 2024-01-24
WO2009052505A1 (en) 2009-04-23
IL283597B (en) 2022-11-01
US9267110B2 (en) 2016-02-23
CN104498432B (zh) 2023-08-11
US20160222346A1 (en) 2016-08-04
EP3375867A1 (en) 2018-09-19
US10808223B2 (en) 2020-10-20
US20210047616A1 (en) 2021-02-18
EP2215214A4 (en) 2012-09-05
CN104498432A (zh) 2015-04-08
IL283597B2 (en) 2023-03-01
IL267371B (en) 2021-06-30
IL267371A (en) 2019-08-29
CN101903515B (zh) 2014-12-10
US10246680B2 (en) 2019-04-02
IL238395A0 (en) 2015-06-30
DK3375867T3 (da) 2024-04-02
US8334138B2 (en) 2012-12-18
US20190225938A1 (en) 2019-07-25
EP4368701A2 (en) 2024-05-15
PL2215214T3 (pl) 2018-08-31
IL297378B1 (en) 2024-11-01
EP2215214B1 (en) 2018-05-16
ES2674239T3 (es) 2018-06-28
US11920154B2 (en) 2024-03-05
AU2008311798A1 (en) 2009-04-23
US8623650B2 (en) 2014-01-07
ES2971288T3 (es) 2024-06-04
US20100311164A1 (en) 2010-12-09
CA2703116A1 (en) 2009-04-23
US20140099713A1 (en) 2014-04-10
US20240218324A1 (en) 2024-07-04
IL283597A (en) 2021-07-29
EP4368701A3 (en) 2024-09-04
AU2008311798B2 (en) 2014-05-01
US20090104696A1 (en) 2009-04-23
EP2215214A1 (en) 2010-08-11
IL238395B (en) 2019-06-30
CN101903515A (zh) 2010-12-01
IL297378A (en) 2022-12-01
IL205115A (en) 2015-10-29
CA2703116C (en) 2019-01-08
IL297378B2 (en) 2025-03-01

Similar Documents

Publication Publication Date Title
ES2674239T8 (es) Medios para células madre pluripotentes carentes de alimentadores que contienen suero humano
DK3192865T3 (da) Enkelt pluripotent stamcelle-kultur
IL254144A0 (en) Methods for reprogramming cells and uses thereof
EP2297182A4 (en) HIGHLY CHARGED PROTEINS USED FOR CELL PENETRATION
BRPI1011127A2 (pt) métodos para o cultivo de células tronco e células progenitoras
EP2142641A4 (en) UNDIFFERENCED STEM CELL CULTURAL SYSTEMS
BRPI1011936A2 (pt) métodos biológicos para a preparação de ácido adípico
EP2419166A4 (en) IMPLANT PLACEMENT SYSTEM
EP2419041A4 (en) IMPLANT PLACEMENT SYSTEM
FI20096288A0 (fi) Formulations and methods for culturing stem cells
HRP20171823T1 (hr) Postupak izdvajanja nukleinskih kiselina i komplet u tu svrhu
DK2185693T3 (da) Differentiering af humane embryoniske stamceller
BRPI1009981A2 (pt) sistemas , métodos e composições para otimizar os enxertos enriquecidos de tecido e célula
PL2046946T3 (pl) Hodowla pluripotencjalnych komórek macierzystych
ZA201001158B (en) Diferentiation of human embryonic stem cells
BRPI1010540A2 (pt) "anticorpos especéficos para dkk-1 e seus usos"
BRPI1008219A2 (pt) Biorreator para o cultivo de células de mamíferos
PL3287525T3 (pl) Preparaty RNA zawierające oczyszczony zmodyfikowany RNA do przeprogramowywania komórek
BRPI0919058A2 (pt) indicador biológico independente
IL265409A (en) Antibodies and processes for preparing the same
BRPI0913925A2 (pt) Diferenciação de células-tronco pluripotentes
BRPI0812040A2 (pt) Indicações para terapia de anti-il-i-beta
BRPI0907831A2 (pt) composição para modular o crescimento de célula-tronco com prostaglandinas
IL206588A0 (en) Device for the study of living cells
IL205470A0 (en) Methods for co-culturing cord blood derived cells with menstrual stem cells