ES2615644T3 - Aparato de extracción de ácido nucleico a velocidad ultra-alta y procedimiento de extracción de ácido nucleico usando el mismo - Google Patents

Aparato de extracción de ácido nucleico a velocidad ultra-alta y procedimiento de extracción de ácido nucleico usando el mismo Download PDF

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ES2615644T3
ES2615644T3 ES13745997.0T ES13745997T ES2615644T3 ES 2615644 T3 ES2615644 T3 ES 2615644T3 ES 13745997 T ES13745997 T ES 13745997T ES 2615644 T3 ES2615644 T3 ES 2615644T3
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nucleic acid
filter
chip
acid extraction
extraction
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Sung Woo Kim
Duck Joong Kim
Dong Hoon Lee
Sun Jin Kim
Yong Hea CHOI
Ho Sun Ryu
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Nanobiosys Inc
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Nanobiosys Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0681—Filter
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/40—Apparatus specially designed for the use of free, immobilised, or carrier-bound enzymes, e.g. apparatus containing a fluidised bed of immobilised enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/101—Temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/629—Detection means characterised by use of a special device being a microfluidic device
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/34—Purifying; Cleaning

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
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  • Organic Chemistry (AREA)
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  • Wood Science & Technology (AREA)
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  • Biotechnology (AREA)
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  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Clinical Laboratory Science (AREA)
  • Hematology (AREA)
  • Dispersion Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Sustainable Development (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Un dispositivo para la extracción de un ácido nucleico de un espécimen biológico, que comprende: 5 un sustrato (500); un soporte del chip (600) que está dispuesto en la superficie superior del sustrato (500) y que incluye una parte de recepción (650) del chip que tiene la forma de una placa con una superficie horizontal en estrecho contacto con una superficie inferior de un chip de microfluidos para la extracción de ácidos nucleicos; un calentador (700) en forma de un bloque de calentamiento de tipo placa de contacto, estando el calentador (700) dispuesto en la superficie horizontal de la parte de recepción (650) del chip y hecho para aplicar calor a una parte de toda la superficie inferior del chip de microfluidos (1) para la extracción de ácidos nucleicos instalada en la parte de recepción (650) del chip; y un chip de microfluidos (1) para la extracción de ácidos nucleicos que tiene la forma de una placa con una superficie horizontal superior y una superficie inferior horizontal, en el que el chip de microfluidos (1) comprende: una parte de entrada (10); para recibir un espécimen biológico una parte de salida (60) para recuperar el ácido nucleico extraído; un canal (70) que conecta la parte de entrada (10) y la parte de salida (60); un primer filtro (30) y un segundo filtro (50), en el que el primer filtro (30) y el segundo filtro (50) están dispuestos dentro del canal (70) y en el que el primer filtro (30) y el segundo filtro (50) están configurados para permitir que una sustancia que tiene un tamaño equivalente de un ácido nucleico pase a través; una parte de separación (40) de ácido nucleico, en la que la parte de separación (40) de ácido nucleico está situada entre el primer filtro (30) y el segundo filtro (50), y en la que dicha parte de separación (40) de ácido nucleico comprende una sustancia de unión (45) a ácido nucleico capaz de unirse específicamente al ácido nucleico; y una parte de calentamiento (20), en la que la parte de calentamiento (20) está dispuesta dentro del canal (70) y está situada entre la parte de entrada (10) y el primer filtro (30), en la que la parte de calentamiento (20) está configurada para transferir el calor obtenido del calentador (700) a un espécimen biológico introducido a través de la parte de entrada (10).

Description

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Claims (1)

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ES13745997.0T 2012-02-07 2013-01-25 Aparato de extracción de ácido nucleico a velocidad ultra-alta y procedimiento de extracción de ácido nucleico usando el mismo Active ES2615644T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
KR1020120012222A KR101915675B1 (ko) 2012-02-07 2012-02-07 초고속 핵산 추출 장치, 및 이를 이용하는 핵산 추출 방법
KR20120012222 2012-02-07
PCT/KR2013/000609 WO2013118990A1 (ko) 2012-02-07 2013-01-25 초고속 핵산 추출 장치, 및 이를 이용하는 핵산 추출 방법

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ES2615644T3 true ES2615644T3 (es) 2017-06-07

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ES13745997.0T Active ES2615644T3 (es) 2012-02-07 2013-01-25 Aparato de extracción de ácido nucleico a velocidad ultra-alta y procedimiento de extracción de ácido nucleico usando el mismo

Country Status (11)

Country Link
US (1) US9476038B2 (es)
EP (1) EP2813565B1 (es)
JP (1) JP6062963B2 (es)
KR (1) KR101915675B1 (es)
CN (1) CN104114687B (es)
DK (1) DK2813565T3 (es)
ES (1) ES2615644T3 (es)
HU (1) HUE031771T2 (es)
PT (1) PT2813565T (es)
RS (1) RS55711B1 (es)
WO (1) WO2013118990A1 (es)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
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CN110199859B (zh) * 2019-06-15 2021-02-23 浙江大学 用于研究根际微域的方法及所用的微流控芯片装置
KR20230155183A (ko) * 2022-05-03 2023-11-10 경북대학교 산학협력단 혈액 내 핵산 분리를 위한 휴대 가능 장치

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Publication number Publication date
CN104114687B (zh) 2016-06-22
DK2813565T3 (en) 2017-02-27
PT2813565T (pt) 2017-02-23
WO2013118990A1 (ko) 2013-08-15
KR20130101606A (ko) 2013-09-16
US20140349386A1 (en) 2014-11-27
JP6062963B2 (ja) 2017-01-18
RS55711B1 (sr) 2017-07-31
EP2813565A4 (en) 2015-08-12
EP2813565B1 (en) 2016-11-16
CN104114687A (zh) 2014-10-22
US9476038B2 (en) 2016-10-25
HUE031771T2 (en) 2017-07-28
KR101915675B1 (ko) 2018-11-06
JP2015507918A (ja) 2015-03-16
EP2813565A1 (en) 2014-12-17

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