ES2570570T3 - Método para determinar la constante de unión de compuestos de alta afinidad - Google Patents

Método para determinar la constante de unión de compuestos de alta afinidad

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Publication number
ES2570570T3
ES2570570T3 ES11706799T ES11706799T ES2570570T3 ES 2570570 T3 ES2570570 T3 ES 2570570T3 ES 11706799 T ES11706799 T ES 11706799T ES 11706799 T ES11706799 T ES 11706799T ES 2570570 T3 ES2570570 T3 ES 2570570T3
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Spain
Prior art keywords
compound
interest
affinity
protein
sink
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Active
Application number
ES11706799T
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English (en)
Inventor
Frauke Assmus
Holger Fischer
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F Hoffmann La Roche AG
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F Hoffmann La Roche AG
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Publication date
Application filed by F Hoffmann La Roche AG filed Critical F Hoffmann La Roche AG
Application granted granted Critical
Publication of ES2570570T3 publication Critical patent/ES2570570T3/es
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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08H—DERIVATIVES OF NATURAL MACROMOLECULAR COMPOUNDS
    • C08H1/00—Macromolecular products derived from proteins
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
    • B01D61/24—Dialysis ; Membrane extraction
    • B01D61/243—Dialysis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
    • B01D61/24—Dialysis ; Membrane extraction
    • B01D61/28—Apparatus therefor
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5025—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures for parallel transport of multiple samples
    • B01L3/50255—Multi-well filtration
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/49—Blood
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5302—Apparatus specially adapted for immunological test procedures
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0609—Holders integrated in container to position an object
    • B01L2300/0618—Holders integrated in container to position an object for removable separation walls
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0829—Multi-well plates; Microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0848—Specific forms of parts of containers
    • B01L2300/0858—Side walls

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Water Supply & Treatment (AREA)
  • Clinical Laboratory Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Materials Engineering (AREA)
  • Polymers & Plastics (AREA)
  • Ecology (AREA)
  • Biophysics (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Método para determinar la constante de unión de un compuesto de alta afinidad de interés a proteínas, comprendiendo las etapas siguientes: a) adición de compuesto de alta afinidad a un sistema de dos cámaras, en el que las dos cámaras están separadas por una membrana semipermeable, que es permeable al compuesto de interés, y determinación de la cantidad del compuesto de alta afinidad de interés en una de las cámaras tras alcanzarse el equilibrio de distribución, b) adición de un agente acomplejante como compuesto sumidero a una de las cámaras, en el que el compuesto sumidero es una sustancia que reduce significativamente la afinidad del compuesto de interés para una proteína no específica; dicha proteína no específica se une a un amplio abanico de compuestos y el compuesto sumidero no permea la membrana, y determinación del coeficiente de distribución del compuesto de interés respecto al compuesto sumidero tras alcanzarse el equilibrio de distribución, c) adición de una proteína del plasma como proteína no específica a la otra cámara, en la que la proteína del plasma no puede permear la membrana, y determinación del coeficiente de distribución del compuesto de interés respecto a la proteína del plasma en presencia de un compuesto sumidero tras alcanzarse el equilibrio de distribución, y d) determinación de la constante de unión del compuesto de alta afinidad de interés con el coeficiente de distribución de las etapas b) y c), y en la que el compuesto de alta afinidad de interés se refiere a un compuesto que bajo condiciones fisiológicas y en presencia de un exceso de proteína del plasma, por lo menos el 95% de la cantidad total del mismo se encuentra unida a dicha proteína del plasma.
ES11706799T 2010-02-25 2011-02-22 Método para determinar la constante de unión de compuestos de alta afinidad Active ES2570570T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP10154696 2010-02-25
PCT/EP2011/052550 WO2011104210A1 (en) 2010-02-25 2011-02-22 Method for determining the binding constant of high affinity compounds

Publications (1)

Publication Number Publication Date
ES2570570T3 true ES2570570T3 (es) 2016-05-19

Family

ID=43836798

Family Applications (1)

Application Number Title Priority Date Filing Date
ES11706799T Active ES2570570T3 (es) 2010-02-25 2011-02-22 Método para determinar la constante de unión de compuestos de alta afinidad

Country Status (12)

Country Link
US (2) US8778617B2 (es)
EP (1) EP2539051B1 (es)
JP (1) JP5637608B2 (es)
KR (1) KR101521688B1 (es)
CN (1) CN102770195B (es)
BR (1) BR112012016061A2 (es)
CA (1) CA2788054C (es)
ES (1) ES2570570T3 (es)
HK (1) HK1177710A1 (es)
MX (1) MX343375B (es)
RU (1) RU2573505C2 (es)
WO (1) WO2011104210A1 (es)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5637608B2 (ja) * 2010-02-25 2014-12-10 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 高親和性化合物の結合定数を求めるための方法
US20240125807A1 (en) * 2020-12-18 2024-04-18 Chugai Seiyaku Kabushiki Kaisha METHOD FOR MEASURING RELATIVE fu RATIO BY DYNAMIC ANALYSIS
US20230132578A1 (en) * 2021-11-04 2023-05-04 Pall Corporation Multiple well device and method of use

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1088589A3 (en) * 1999-09-30 2003-06-18 Pfizer Products Inc. Micro-equilibrium dialysis vertically loaded apparatus
DE19952568C2 (de) * 1999-11-02 2003-05-22 Noxxon Pharma Ag Fluoreszenzmarkierte Nukleinsäure-Liganden
GB0003234D0 (en) * 2000-02-11 2000-04-05 Pfizer Ltd Method of treatment
DE10012120A1 (de) * 2000-03-13 2001-09-27 Ktb Tumorforschungs Gmbh Therapeutische und diagnostische Ligandensysteme mit Transportmolekülbindenden Eigenschaften und diese enthaltende Arzneimittel
CN1170138C (zh) * 2001-02-23 2004-10-06 国家药品监督管理局天津药物研究院 平衡透析装置
US20030213740A1 (en) 2001-06-05 2003-11-20 Andrew Creasey Multi-well equilibrium dialysis systems
JP2004303186A (ja) * 2003-03-20 2004-10-28 Sumitomo Pharmaceut Co Ltd 蛋白結合率の予測に関する装置およびその方法
CN1331608C (zh) * 2005-08-02 2007-08-15 天津大学 中空膜管式微萃取分析器及其应用方法
US20070215538A1 (en) * 2006-03-20 2007-09-20 Harvard Apparatus, Inc. Systems and methods for equilibrium dialysis
EP1894924A1 (en) * 2006-08-29 2008-03-05 Phenex Pharmaceuticals AG Heterocyclic FXR binding compounds
CN1939348A (zh) * 2006-09-22 2007-04-04 中国药科大学 一种中药有效成分的筛选方法
JP5637608B2 (ja) * 2010-02-25 2014-12-10 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 高親和性化合物の結合定数を求めるための方法

Also Published As

Publication number Publication date
US20120309109A1 (en) 2012-12-06
HK1177710A1 (en) 2013-08-30
US20140273279A1 (en) 2014-09-18
RU2573505C2 (ru) 2016-01-20
CA2788054C (en) 2018-08-14
EP2539051B1 (en) 2016-03-30
BR112012016061A2 (pt) 2020-09-08
US8778617B2 (en) 2014-07-15
KR101521688B1 (ko) 2015-05-19
CA2788054A1 (en) 2011-09-01
WO2011104210A1 (en) 2011-09-01
MX2012009185A (es) 2012-08-23
CN102770195A (zh) 2012-11-07
KR20120123107A (ko) 2012-11-07
CN102770195B (zh) 2015-03-04
EP2539051A1 (en) 2013-01-02
RU2012139183A (ru) 2014-03-27
MX343375B (es) 2016-11-03
JP5637608B2 (ja) 2014-12-10
JP2013519078A (ja) 2013-05-23

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