ES2565379T3 - Método para aislar osteopontina usando alimentaciones que contienen caseinomacropéptido (CMP) - Google Patents
Método para aislar osteopontina usando alimentaciones que contienen caseinomacropéptido (CMP) Download PDFInfo
- Publication number
- ES2565379T3 ES2565379T3 ES12706623.1T ES12706623T ES2565379T3 ES 2565379 T3 ES2565379 T3 ES 2565379T3 ES 12706623 T ES12706623 T ES 12706623T ES 2565379 T3 ES2565379 T3 ES 2565379T3
- Authority
- ES
- Spain
- Prior art keywords
- milk
- feed
- protein
- range
- weight
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 102000004264 Osteopontin Human genes 0.000 title abstract description 41
- 108010081689 Osteopontin Proteins 0.000 title abstract description 41
- 238000000034 method Methods 0.000 title abstract description 23
- 108010067454 caseinomacropeptide Proteins 0.000 title abstract 3
- 235000013336 milk Nutrition 0.000 abstract description 76
- 239000008267 milk Substances 0.000 abstract description 76
- 210000004080 milk Anatomy 0.000 abstract description 76
- 102000004169 proteins and genes Human genes 0.000 abstract description 54
- 108090000623 proteins and genes Proteins 0.000 abstract description 54
- 238000005349 anion exchange Methods 0.000 abstract description 17
- 239000000203 mixture Substances 0.000 abstract description 2
- 238000005571 anion exchange chromatography Methods 0.000 abstract 1
- 235000018102 proteins Nutrition 0.000 description 52
- 108010046377 Whey Proteins Proteins 0.000 description 29
- 102000007544 Whey Proteins Human genes 0.000 description 27
- 239000005862 Whey Substances 0.000 description 24
- 102000011632 Caseins Human genes 0.000 description 22
- 108010076119 Caseins Proteins 0.000 description 22
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 20
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 15
- 235000021240 caseins Nutrition 0.000 description 15
- 239000005018 casein Substances 0.000 description 10
- 239000002994 raw material Substances 0.000 description 10
- 239000011780 sodium chloride Substances 0.000 description 10
- 101000961187 Anacardium occidentale 11S globulin seed storage protein Ana o 2.0101 Proteins 0.000 description 9
- 102100033668 Cartilage matrix protein Human genes 0.000 description 9
- 101001018382 Homo sapiens Cartilage matrix protein Proteins 0.000 description 9
- 235000009508 confectionery Nutrition 0.000 description 8
- 238000002474 experimental method Methods 0.000 description 7
- 238000004128 high performance liquid chromatography Methods 0.000 description 7
- 108090000942 Lactalbumin Proteins 0.000 description 6
- 102000004407 Lactalbumin Human genes 0.000 description 6
- 102000008192 Lactoglobulins Human genes 0.000 description 6
- 108010060630 Lactoglobulins Proteins 0.000 description 6
- 238000004519 manufacturing process Methods 0.000 description 6
- 239000000243 solution Substances 0.000 description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- 235000021241 α-lactalbumin Nutrition 0.000 description 6
- 229940021722 caseins Drugs 0.000 description 5
- 238000001556 precipitation Methods 0.000 description 5
- 150000003839 salts Chemical class 0.000 description 5
- 235000021119 whey protein Nutrition 0.000 description 5
- 235000021247 β-casein Nutrition 0.000 description 5
- 239000000693 micelle Substances 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 239000011347 resin Substances 0.000 description 4
- 229920005989 resin Polymers 0.000 description 4
- 238000005406 washing Methods 0.000 description 4
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 239000012141 concentrate Substances 0.000 description 3
- 238000002955 isolation Methods 0.000 description 3
- 239000008363 phosphate buffer Substances 0.000 description 3
- 238000011002 quantification Methods 0.000 description 3
- 238000007696 Kjeldahl method Methods 0.000 description 2
- 239000012901 Milli-Q water Substances 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 238000011088 calibration curve Methods 0.000 description 2
- 238000011026 diafiltration Methods 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 238000005227 gel permeation chromatography Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000011068 loading method Methods 0.000 description 2
- 238000001471 micro-filtration Methods 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 235000020183 skimmed milk Nutrition 0.000 description 2
- 235000021249 α-casein Nutrition 0.000 description 2
- 102000004506 Blood Proteins Human genes 0.000 description 1
- 108010017384 Blood Proteins Proteins 0.000 description 1
- 239000012614 Q-Sepharose Substances 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000003957 anion exchange resin Substances 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 235000013365 dairy product Nutrition 0.000 description 1
- 238000011033 desalting Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000003480 eluent Substances 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 238000010812 external standard method Methods 0.000 description 1
- 238000005189 flocculation Methods 0.000 description 1
- 230000016615 flocculation Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000000164 protein isolation Methods 0.000 description 1
- 238000001742 protein purification Methods 0.000 description 1
- 229940108461 rennet Drugs 0.000 description 1
- 108010058314 rennet Proteins 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 230000001953 sensory effect Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 1
- 238000005063 solubilization Methods 0.000 description 1
- 230000007928 solubilization Effects 0.000 description 1
- 239000012086 standard solution Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000000108 ultra-filtration Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/20—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from milk, e.g. casein; from whey
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/18—Ion-exchange chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Biochemistry (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Zoology (AREA)
- Analytical Chemistry (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Peptides Or Proteins (AREA)
- Dairy Products (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161448775P | 2011-03-03 | 2011-03-03 | |
| EP11156826 | 2011-03-03 | ||
| EP11156826 | 2011-03-03 | ||
| US201161448775P | 2011-03-03 | ||
| PCT/EP2012/053748 WO2012117119A1 (en) | 2011-03-03 | 2012-03-05 | Method for isolating osteopontin using feeds containing cmp or casein species |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2565379T3 true ES2565379T3 (es) | 2016-04-04 |
Family
ID=44202290
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES12707103.3T Active ES2565380T3 (es) | 2011-03-03 | 2012-03-05 | Método para aislar osteopontina usando alimentaciones concentradas |
| ES12706623.1T Active ES2565379T3 (es) | 2011-03-03 | 2012-03-05 | Método para aislar osteopontina usando alimentaciones que contienen caseinomacropéptido (CMP) |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES12707103.3T Active ES2565380T3 (es) | 2011-03-03 | 2012-03-05 | Método para aislar osteopontina usando alimentaciones concentradas |
Country Status (15)
| Country | Link |
|---|---|
| US (2) | US9181316B2 (enExample) |
| EP (2) | EP2681230B1 (enExample) |
| JP (2) | JP6012053B2 (enExample) |
| KR (2) | KR101940829B1 (enExample) |
| CN (3) | CN105085649B (enExample) |
| AR (2) | AR088719A1 (enExample) |
| AU (2) | AU2012222312B2 (enExample) |
| BR (2) | BR112013022252B1 (enExample) |
| CA (2) | CA2828746C (enExample) |
| DK (2) | DK2681231T3 (enExample) |
| EA (2) | EA023367B1 (enExample) |
| ES (2) | ES2565380T3 (enExample) |
| MX (2) | MX2013010017A (enExample) |
| PL (2) | PL2681230T3 (enExample) |
| WO (2) | WO2012117119A1 (enExample) |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103797682B (zh) * | 2011-04-28 | 2017-08-25 | 佐尔循环公司 | 电池管理参数的病毒式分布 |
| WO2013144247A1 (en) | 2012-03-28 | 2013-10-03 | Arla Foods Amba | Nanoparticle aggregates containing osteopontin and calcium- and/or strontium-containing particles |
| AU2014286123B2 (en) * | 2013-07-05 | 2019-02-28 | Arla Foods Amba | Mammalian milk osteopontin for enhancing immune responsiveness |
| US9661868B2 (en) * | 2013-07-16 | 2017-05-30 | Mead Johnson Nutrition Company | Expanded bed adsorption methods for isolation of basic milk proteins including lactoferrin |
| KR200482960Y1 (ko) | 2016-04-07 | 2017-03-21 | 오숙희 | 고주파 얼굴 축소 시술기 |
| JP2019520856A (ja) * | 2016-07-06 | 2019-07-25 | ビルディング ブロック ニュートリショナルズ，エルエルシーＢｕｉｌｄｉｎｇ Ｂｌｏｃｋ Ｎｕｔｒｉｔｉｏｎａｌｓ， Ｌｌｃ | 栄養処方 |
| EP3538053A1 (en) | 2016-11-08 | 2019-09-18 | Dentherapy Ltd | Compositions comprising fluoride and calcium and method for preparing them |
| AU2018386652A1 (en) * | 2017-12-22 | 2020-05-07 | Societe Des Produits Nestle S.A. | Extracts and their use |
| CN109613140B (zh) * | 2019-01-07 | 2021-10-08 | 雅士利国际集团有限公司 | 食品中酪蛋白磷酸肽的检测方法 |
| IL299098A (en) | 2020-06-15 | 2023-02-01 | Building Block Nutritionals Llc | Nutritional formula |
| GB202010987D0 (en) | 2020-07-16 | 2020-09-02 | Dentherapy Ltd | Oral care compositions and methods |
| WO2022223427A1 (en) * | 2021-04-19 | 2022-10-27 | Société des Produits Nestlé S.A. | Use of milk protein fractions as a source of osteopontin |
| CN113567573B (zh) * | 2021-07-09 | 2023-06-02 | 黑龙江飞鹤乳业有限公司 | 骨桥蛋白的分离方法和分析方法 |
| GB202110114D0 (en) | 2021-07-14 | 2021-08-25 | Dentherapy Ltd | Oral care compositions for use in treaatment |
| WO2024056840A1 (en) | 2022-09-16 | 2024-03-21 | Univerza V Ljubljani | Isolation of osteopontin and glycomacropeptide from whey |
| WO2025008636A1 (en) | 2023-07-06 | 2025-01-09 | Dentherapy Ltd | Oral care compositions for use in the treatment of dental health conditions |
| CN117837766A (zh) * | 2023-12-27 | 2024-04-09 | 美维仕(北京)健康管理有限公司 | 营养组合物及其应用 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0963163A4 (en) | 1996-10-01 | 2004-09-08 | Univ Massey | Process for isolating glycomacropeptide from dairy products with a phenylalanine impurity of 0.5 %w/w |
| AU748002B2 (en) | 1997-12-31 | 2002-05-30 | Depuy Orthopaedics, Inc. | Osteopontin-based compositions for enhancing bone repair |
| KR20010043323A (ko) | 1998-05-06 | 2001-05-25 | 제넨테크, 인크. | 이온 교환 크로마토그래피에 의한 단백질 정제 방법 |
| US7018665B2 (en) * | 1999-12-08 | 2006-03-28 | Massey University | Process for separation of whey proteins using a novel anion exchanger |
| US7259243B2 (en) | 2000-01-07 | 2007-08-21 | Arla Foods Amba | Process for isolation of osteopontin from milk |
| NZ507335A (en) * | 2000-10-05 | 2004-10-29 | New Zealand Dairy Board | Bone health compositions derived from milk comprising an acidic protein fraction but that does not contain CGMP |
-
2012
- 2012-03-05 KR KR1020137026174A patent/KR101940829B1/ko active Active
- 2012-03-05 EP EP12706623.1A patent/EP2681230B1/en active Active
- 2012-03-05 JP JP2013555901A patent/JP6012053B2/ja active Active
- 2012-03-05 AU AU2012222312A patent/AU2012222312B2/en active Active
- 2012-03-05 DK DK12707103.3T patent/DK2681231T3/en active
- 2012-03-05 BR BR112013022252-2A patent/BR112013022252B1/pt active IP Right Grant
- 2012-03-05 US US14/002,578 patent/US9181316B2/en active Active
- 2012-03-05 ES ES12707103.3T patent/ES2565380T3/es active Active
- 2012-03-05 PL PL12706623T patent/PL2681230T3/pl unknown
- 2012-03-05 CN CN201510434329.3A patent/CN105085649B/zh active Active
- 2012-03-05 DK DK12706623.1T patent/DK2681230T3/en active
- 2012-03-05 WO PCT/EP2012/053748 patent/WO2012117119A1/en not_active Ceased
- 2012-03-05 JP JP2013555900A patent/JP5967665B2/ja active Active
- 2012-03-05 CN CN201280015375.4A patent/CN103476787B/zh active Active
- 2012-03-05 PL PL12707103T patent/PL2681231T3/pl unknown
- 2012-03-05 MX MX2013010017A patent/MX2013010017A/es active IP Right Grant
- 2012-03-05 WO PCT/EP2012/053749 patent/WO2012117120A1/en not_active Ceased
- 2012-03-05 KR KR1020137026175A patent/KR101940830B1/ko active Active
- 2012-03-05 ES ES12706623.1T patent/ES2565379T3/es active Active
- 2012-03-05 CA CA2828746A patent/CA2828746C/en active Active
- 2012-03-05 EA EA201391203A patent/EA023367B1/ru not_active IP Right Cessation
- 2012-03-05 CN CN201280015495.4A patent/CN103492408B/zh active Active
- 2012-03-05 EA EA201391204A patent/EA023615B1/ru not_active IP Right Cessation
- 2012-03-05 MX MX2013010019A patent/MX2013010019A/es active IP Right Grant
- 2012-03-05 BR BR112013022251-4A patent/BR112013022251B1/pt active IP Right Grant
- 2012-03-05 US US14/002,614 patent/US9181325B2/en active Active
- 2012-03-05 AU AU2012222313A patent/AU2012222313B2/en active Active
- 2012-03-05 CA CA2828669A patent/CA2828669C/en active Active
- 2012-03-05 EP EP12707103.3A patent/EP2681231B1/en active Active
- 2012-03-06 AR ARP120100714A patent/AR088719A1/es active IP Right Grant
- 2012-03-06 AR ARP120100713A patent/AR085620A1/es active IP Right Grant
Also Published As
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