AU2012222312B2 - Method for isolating osteopontin using feeds containing cmp or casein species - Google Patents
Method for isolating osteopontin using feeds containing cmp or casein species Download PDFInfo
- Publication number
- AU2012222312B2 AU2012222312B2 AU2012222312A AU2012222312A AU2012222312B2 AU 2012222312 B2 AU2012222312 B2 AU 2012222312B2 AU 2012222312 A AU2012222312 A AU 2012222312A AU 2012222312 A AU2012222312 A AU 2012222312A AU 2012222312 B2 AU2012222312 B2 AU 2012222312B2
- Authority
- AU
- Australia
- Prior art keywords
- milk
- derived feed
- protein
- range
- derived
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 102000004264 Osteopontin Human genes 0.000 title claims abstract description 149
- 108010081689 Osteopontin Proteins 0.000 title claims abstract description 149
- 238000000034 method Methods 0.000 title claims abstract description 87
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 title description 30
- 235000021240 caseins Nutrition 0.000 title description 30
- 239000005018 casein Substances 0.000 title description 25
- 235000013336 milk Nutrition 0.000 claims abstract description 280
- 239000008267 milk Substances 0.000 claims abstract description 280
- 210000004080 milk Anatomy 0.000 claims abstract description 280
- 108010046377 Whey Proteins Proteins 0.000 claims abstract description 73
- 102000007544 Whey Proteins Human genes 0.000 claims abstract description 71
- 239000005862 Whey Substances 0.000 claims abstract description 62
- 108010067454 caseinomacropeptide Proteins 0.000 claims abstract description 43
- 235000009508 confectionery Nutrition 0.000 claims abstract description 34
- 210000002966 serum Anatomy 0.000 claims abstract description 28
- 235000018102 proteins Nutrition 0.000 claims description 148
- 102000004169 proteins and genes Human genes 0.000 claims description 148
- 108090000623 proteins and genes Proteins 0.000 claims description 148
- 238000005349 anion exchange Methods 0.000 claims description 72
- 239000000203 mixture Substances 0.000 claims description 29
- 238000005406 washing Methods 0.000 claims description 13
- 238000005571 anion exchange chromatography Methods 0.000 claims description 11
- 102000004407 Lactalbumin Human genes 0.000 claims description 9
- 108090000942 Lactalbumin Proteins 0.000 claims description 9
- 239000007788 liquid Substances 0.000 claims description 9
- 239000002244 precipitate Substances 0.000 claims description 9
- 235000021241 α-lactalbumin Nutrition 0.000 claims description 9
- 102000008192 Lactoglobulins Human genes 0.000 claims description 7
- 108010060630 Lactoglobulins Proteins 0.000 claims description 7
- 238000001704 evaporation Methods 0.000 claims description 5
- 230000008020 evaporation Effects 0.000 claims description 5
- 238000011026 diafiltration Methods 0.000 claims description 4
- 238000001694 spray drying Methods 0.000 claims description 4
- 150000001768 cations Chemical class 0.000 claims description 3
- 239000002904 solvent Substances 0.000 claims description 2
- 238000006467 substitution reaction Methods 0.000 claims description 2
- 108010076119 Caseins Proteins 0.000 abstract description 83
- 102000011632 Caseins Human genes 0.000 abstract description 83
- 235000021247 β-casein Nutrition 0.000 abstract description 33
- 238000002955 isolation Methods 0.000 abstract description 6
- 239000002253 acid Substances 0.000 description 28
- 235000021249 α-casein Nutrition 0.000 description 19
- 239000012141 concentrate Substances 0.000 description 17
- 230000008569 process Effects 0.000 description 15
- 239000002994 raw material Substances 0.000 description 15
- 239000000693 micelle Substances 0.000 description 14
- 238000002474 experimental method Methods 0.000 description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 12
- 239000000243 solution Substances 0.000 description 11
- 235000021119 whey protein Nutrition 0.000 description 11
- 230000000694 effects Effects 0.000 description 10
- 238000004519 manufacturing process Methods 0.000 description 8
- 238000001556 precipitation Methods 0.000 description 8
- 239000000047 product Substances 0.000 description 7
- 239000011347 resin Substances 0.000 description 7
- 229920005989 resin Polymers 0.000 description 7
- 102000004506 Blood Proteins Human genes 0.000 description 6
- 108010017384 Blood Proteins Proteins 0.000 description 6
- 238000012545 processing Methods 0.000 description 6
- 239000007790 solid phase Substances 0.000 description 6
- 230000002378 acidificating effect Effects 0.000 description 5
- 239000007864 aqueous solution Substances 0.000 description 5
- 229940021722 caseins Drugs 0.000 description 5
- 125000002091 cationic group Chemical group 0.000 description 5
- 239000003480 eluent Substances 0.000 description 5
- 238000004128 high performance liquid chromatography Methods 0.000 description 5
- 239000000463 material Substances 0.000 description 5
- 229920001282 polysaccharide Polymers 0.000 description 5
- 108090000765 processed proteins & peptides Proteins 0.000 description 5
- 235000020183 skimmed milk Nutrition 0.000 description 5
- 238000000108 ultra-filtration Methods 0.000 description 5
- 235000013351 cheese Nutrition 0.000 description 4
- 238000001914 filtration Methods 0.000 description 4
- 108010028463 kappa-casein glycomacropeptide Proteins 0.000 description 4
- 102000004196 processed proteins & peptides Human genes 0.000 description 4
- 238000000746 purification Methods 0.000 description 4
- 150000003839 salts Chemical class 0.000 description 4
- 241000894007 species Species 0.000 description 4
- -1 5 0.1 M NaCI Chemical compound 0.000 description 3
- 241000283690 Bos taurus Species 0.000 description 3
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 238000010923 batch production Methods 0.000 description 3
- 230000002308 calcification Effects 0.000 description 3
- 239000011575 calcium Substances 0.000 description 3
- 229910001424 calcium ion Inorganic materials 0.000 description 3
- 230000007423 decrease Effects 0.000 description 3
- 230000003247 decreasing effect Effects 0.000 description 3
- 238000010790 dilution Methods 0.000 description 3
- 239000012895 dilution Substances 0.000 description 3
- 150000004676 glycans Chemical class 0.000 description 3
- 238000002347 injection Methods 0.000 description 3
- 239000007924 injection Substances 0.000 description 3
- 238000011068 loading method Methods 0.000 description 3
- 210000005075 mammary gland Anatomy 0.000 description 3
- 238000001471 micro-filtration Methods 0.000 description 3
- 238000009928 pasteurization Methods 0.000 description 3
- 239000008363 phosphate buffer Substances 0.000 description 3
- 239000005017 polysaccharide Substances 0.000 description 3
- 239000011148 porous material Substances 0.000 description 3
- 239000000843 powder Substances 0.000 description 3
- 238000011002 quantification Methods 0.000 description 3
- 108010058314 rennet Proteins 0.000 description 3
- 239000012465 retentate Substances 0.000 description 3
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 2
- 238000007696 Kjeldahl method Methods 0.000 description 2
- 239000012901 Milli-Q water Substances 0.000 description 2
- 108010001441 Phosphopeptides Proteins 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 239000007983 Tris buffer Substances 0.000 description 2
- 150000001450 anions Chemical class 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 229910052791 calcium Inorganic materials 0.000 description 2
- 238000004364 calculation method Methods 0.000 description 2
- 238000011088 calibration curve Methods 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 238000004587 chromatography analysis Methods 0.000 description 2
- 238000009776 industrial production Methods 0.000 description 2
- 230000006651 lactation Effects 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 235000021243 milk fat Nutrition 0.000 description 2
- 230000007935 neutral effect Effects 0.000 description 2
- 238000010979 pH adjustment Methods 0.000 description 2
- 230000020477 pH reduction Effects 0.000 description 2
- 229920001184 polypeptide Polymers 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 2
- 125000001453 quaternary ammonium group Chemical group 0.000 description 2
- 235000020185 raw untreated milk Nutrition 0.000 description 2
- 230000002829 reductive effect Effects 0.000 description 2
- 229940108461 rennet Drugs 0.000 description 2
- 125000000467 secondary amino group Chemical group [H]N([*:1])[*:2] 0.000 description 2
- 239000012086 standard solution Substances 0.000 description 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
- 235000021246 κ-casein Nutrition 0.000 description 2
- CHRJZRDFSQHIFI-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;styrene Chemical compound C=CC1=CC=CC=C1.C=CC1=CC=CC=C1C=C CHRJZRDFSQHIFI-UHFFFAOYSA-N 0.000 description 1
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- DFYULHRIYLAUJM-UHFFFAOYSA-N 3,4-diiodobenzoic acid Chemical compound OC(=O)C1=CC=C(I)C(I)=C1 DFYULHRIYLAUJM-UHFFFAOYSA-N 0.000 description 1
- 229920000936 Agarose Polymers 0.000 description 1
- 235000002198 Annona diversifolia Nutrition 0.000 description 1
- 102000005701 Calcium-Binding Proteins Human genes 0.000 description 1
- 108010045403 Calcium-Binding Proteins Proteins 0.000 description 1
- 241000282836 Camelus dromedarius Species 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- 241000282994 Cervidae Species 0.000 description 1
- 229920002307 Dextran Polymers 0.000 description 1
- 241000283073 Equus caballus Species 0.000 description 1
- 102000003886 Glycoproteins Human genes 0.000 description 1
- 108090000288 Glycoproteins Proteins 0.000 description 1
- 241000282414 Homo sapiens Species 0.000 description 1
- 241000282842 Lama glama Species 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- CERQOIWHTDAKMF-UHFFFAOYSA-M Methacrylate Chemical compound CC(=C)C([O-])=O CERQOIWHTDAKMF-UHFFFAOYSA-M 0.000 description 1
- 102000014171 Milk Proteins Human genes 0.000 description 1
- 108010011756 Milk Proteins Proteins 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- 206010029148 Nephrolithiasis Diseases 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- 239000012614 Q-Sepharose Substances 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 238000004220 aggregation Methods 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 125000003277 amino group Chemical group 0.000 description 1
- 239000003957 anion exchange resin Substances 0.000 description 1
- 125000000129 anionic group Chemical group 0.000 description 1
- SQVRNKJHWKZAKO-UHFFFAOYSA-N beta-N-Acetyl-D-neuraminic acid Natural products CC(=O)NC1C(O)CC(O)(C(O)=O)OC1C(O)C(O)CO SQVRNKJHWKZAKO-UHFFFAOYSA-N 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 230000031018 biological processes and functions Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 230000010072 bone remodeling Effects 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 159000000007 calcium salts Chemical class 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 230000015271 coagulation Effects 0.000 description 1
- 238000005345 coagulation Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 238000002425 crystallisation Methods 0.000 description 1
- 230000008025 crystallization Effects 0.000 description 1
- 235000013365 dairy product Nutrition 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000011033 desalting Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000001627 detrimental effect Effects 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 230000003292 diminished effect Effects 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000010812 external standard method Methods 0.000 description 1
- 238000005189 flocculation Methods 0.000 description 1
- 230000016615 flocculation Effects 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 238000005227 gel permeation chromatography Methods 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 238000004255 ion exchange chromatography Methods 0.000 description 1
- 239000003456 ion exchange resin Substances 0.000 description 1
- 229920003303 ion-exchange polymer Polymers 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 238000001155 isoelectric focusing Methods 0.000 description 1
- 238000004811 liquid chromatography Methods 0.000 description 1
- 230000023247 mammary gland development Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 235000021239 milk protein Nutrition 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000001728 nano-filtration Methods 0.000 description 1
- 230000036961 partial effect Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000012466 permeate Substances 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 235000004252 protein component Nutrition 0.000 description 1
- 238000001742 protein purification Methods 0.000 description 1
- 229940024999 proteolytic enzymes for treatment of wounds and ulcers Drugs 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 230000001953 sensory effect Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- SQVRNKJHWKZAKO-OQPLDHBCSA-N sialic acid Chemical compound CC(=O)N[C@@H]1[C@@H](O)C[C@@](O)(C(O)=O)OC1[C@H](O)[C@H](O)CO SQVRNKJHWKZAKO-OQPLDHBCSA-N 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 1
- 235000020202 standardised milk Nutrition 0.000 description 1
- 239000012609 strong anion exchange resin Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 125000001302 tertiary amino group Chemical group 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 230000001131 transforming effect Effects 0.000 description 1
- 210000001635 urinary tract Anatomy 0.000 description 1
- 235000008939 whole milk Nutrition 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/20—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from milk, e.g. casein; from whey
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/18—Ion-exchange chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Biochemistry (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Zoology (AREA)
- Analytical Chemistry (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Peptides Or Proteins (AREA)
- Dairy Products (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161448775P | 2011-03-03 | 2011-03-03 | |
| EP11156826 | 2011-03-03 | ||
| US61/448,775 | 2011-03-03 | ||
| EP11156826.7 | 2011-03-03 | ||
| PCT/EP2012/053748 WO2012117119A1 (en) | 2011-03-03 | 2012-03-05 | Method for isolating osteopontin using feeds containing cmp or casein species |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| AU2012222312A1 AU2012222312A1 (en) | 2013-09-19 |
| AU2012222312B2 true AU2012222312B2 (en) | 2017-06-08 |
Family
ID=44202290
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2012222312A Active AU2012222312B2 (en) | 2011-03-03 | 2012-03-05 | Method for isolating osteopontin using feeds containing cmp or casein species |
| AU2012222313A Active AU2012222313B2 (en) | 2011-03-03 | 2012-03-05 | Method for isolating osteopontin using concentrated feeds |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2012222313A Active AU2012222313B2 (en) | 2011-03-03 | 2012-03-05 | Method for isolating osteopontin using concentrated feeds |
Country Status (15)
| Country | Link |
|---|---|
| US (2) | US9181325B2 (enExample) |
| EP (2) | EP2681231B1 (enExample) |
| JP (2) | JP6012053B2 (enExample) |
| KR (2) | KR101940829B1 (enExample) |
| CN (3) | CN103476787B (enExample) |
| AR (2) | AR088719A1 (enExample) |
| AU (2) | AU2012222312B2 (enExample) |
| BR (2) | BR112013022252B1 (enExample) |
| CA (2) | CA2828746C (enExample) |
| DK (2) | DK2681231T3 (enExample) |
| EA (2) | EA023615B1 (enExample) |
| ES (2) | ES2565380T3 (enExample) |
| MX (2) | MX2013010019A (enExample) |
| PL (2) | PL2681231T3 (enExample) |
| WO (2) | WO2012117120A1 (enExample) |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107257152B (zh) * | 2011-04-28 | 2021-04-09 | 佐尔循环公司 | 用于更新数据、操作参数和/或软件的系统 |
| EA201491691A1 (ru) | 2012-03-28 | 2015-04-30 | Арла Фудз Амба | Агрегаты наночастиц, содержащие остеопонтин и кальций- и/или стронцийсодержащие частицы |
| NZ715430A (en) * | 2013-07-05 | 2022-01-28 | Arla Foods Amba | Mammalian milk osteopontin for enhancing immune responsiveness |
| US9661868B2 (en) * | 2013-07-16 | 2017-05-30 | Mead Johnson Nutrition Company | Expanded bed adsorption methods for isolation of basic milk proteins including lactoferrin |
| KR200482960Y1 (ko) | 2016-04-07 | 2017-03-21 | 오숙희 | 고주파 얼굴 축소 시술기 |
| CN109906040A (zh) * | 2016-07-06 | 2019-06-18 | 积木营养品有限公司 | 营养配方食品 |
| US12109284B2 (en) | 2016-11-08 | 2024-10-08 | Dentherapy Ltd | Compositions comprising fluoride and calcium and method for preparing them |
| US20200404946A1 (en) * | 2017-12-22 | 2020-12-31 | Societe Des Produits Nestle Nestle S.A. | Extracts and their use |
| CN109613140B (zh) * | 2019-01-07 | 2021-10-08 | 雅士利国际集团有限公司 | 食品中酪蛋白磷酸肽的检测方法 |
| CA3182755A1 (en) | 2020-06-15 | 2021-12-23 | James W. McGrath Jr. | Nutritional formula |
| GB202010987D0 (en) | 2020-07-16 | 2020-09-02 | Dentherapy Ltd | Oral care compositions and methods |
| CN117377391A (zh) * | 2021-04-19 | 2024-01-09 | 雀巢产品有限公司 | 乳蛋白质级分作为骨桥蛋白源的用途 |
| CN113567573B (zh) * | 2021-07-09 | 2023-06-02 | 黑龙江飞鹤乳业有限公司 | 骨桥蛋白的分离方法和分析方法 |
| GB202110114D0 (en) | 2021-07-14 | 2021-08-25 | Dentherapy Ltd | Oral care compositions for use in treaatment |
| EP4587455A1 (en) | 2022-09-16 | 2025-07-23 | Univerza V Ljubljani | Isolation of osteopontin and glycomacropeptide from whey |
| WO2025008636A1 (en) | 2023-07-06 | 2025-01-09 | Dentherapy Ltd | Oral care compositions for use in the treatment of dental health conditions |
| CN117837766A (zh) * | 2023-12-27 | 2024-04-09 | 美维仕(北京)健康管理有限公司 | 营养组合物及其应用 |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001049741A2 (en) * | 2000-01-07 | 2001-07-12 | Arla Foods Amba | A process for isolation of osteopontin from milk |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1998014071A1 (en) * | 1996-10-01 | 1998-04-09 | John Stephen Ayers | Process for isolating glycomacropeptide from dairy products with a phenylalanine impurity of 0.5 %w/w |
| AU748002B2 (en) | 1997-12-31 | 2002-05-30 | Depuy Orthopaedics, Inc. | Osteopontin-based compositions for enhancing bone repair |
| ATE321066T1 (de) * | 1998-05-06 | 2006-04-15 | Genentech Inc | Anti-her2 antikörperzusammensetzung |
| CA2393270A1 (en) * | 1999-12-08 | 2001-06-14 | Massey University | Process for separation of whey proteins using a novel anion exchanger |
| NZ507335A (en) | 2000-10-05 | 2004-10-29 | New Zealand Dairy Board | Bone health compositions derived from milk comprising an acidic protein fraction but that does not contain CGMP |
-
2012
- 2012-03-05 EP EP12707103.3A patent/EP2681231B1/en active Active
- 2012-03-05 MX MX2013010019A patent/MX2013010019A/es active IP Right Grant
- 2012-03-05 DK DK12707103.3T patent/DK2681231T3/en active
- 2012-03-05 PL PL12707103T patent/PL2681231T3/pl unknown
- 2012-03-05 ES ES12707103.3T patent/ES2565380T3/es active Active
- 2012-03-05 MX MX2013010017A patent/MX2013010017A/es active IP Right Grant
- 2012-03-05 DK DK12706623.1T patent/DK2681230T3/en active
- 2012-03-05 KR KR1020137026174A patent/KR101940829B1/ko active Active
- 2012-03-05 JP JP2013555901A patent/JP6012053B2/ja active Active
- 2012-03-05 AU AU2012222312A patent/AU2012222312B2/en active Active
- 2012-03-05 PL PL12706623T patent/PL2681230T3/pl unknown
- 2012-03-05 CA CA2828746A patent/CA2828746C/en active Active
- 2012-03-05 BR BR112013022252-2A patent/BR112013022252B1/pt active IP Right Grant
- 2012-03-05 EP EP12706623.1A patent/EP2681230B1/en active Active
- 2012-03-05 AU AU2012222313A patent/AU2012222313B2/en active Active
- 2012-03-05 WO PCT/EP2012/053749 patent/WO2012117120A1/en not_active Ceased
- 2012-03-05 CN CN201280015375.4A patent/CN103476787B/zh active Active
- 2012-03-05 JP JP2013555900A patent/JP5967665B2/ja active Active
- 2012-03-05 CA CA2828669A patent/CA2828669C/en active Active
- 2012-03-05 WO PCT/EP2012/053748 patent/WO2012117119A1/en not_active Ceased
- 2012-03-05 EA EA201391204A patent/EA023615B1/ru not_active IP Right Cessation
- 2012-03-05 US US14/002,614 patent/US9181325B2/en active Active
- 2012-03-05 US US14/002,578 patent/US9181316B2/en active Active
- 2012-03-05 BR BR112013022251-4A patent/BR112013022251B1/pt active IP Right Grant
- 2012-03-05 CN CN201510434329.3A patent/CN105085649B/zh active Active
- 2012-03-05 EA EA201391203A patent/EA023367B1/ru not_active IP Right Cessation
- 2012-03-05 KR KR1020137026175A patent/KR101940830B1/ko active Active
- 2012-03-05 ES ES12706623.1T patent/ES2565379T3/es active Active
- 2012-03-05 CN CN201280015495.4A patent/CN103492408B/zh active Active
- 2012-03-06 AR ARP120100714A patent/AR088719A1/es active IP Right Grant
- 2012-03-06 AR ARP120100713A patent/AR085620A1/es active IP Right Grant
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001049741A2 (en) * | 2000-01-07 | 2001-07-12 | Arla Foods Amba | A process for isolation of osteopontin from milk |
Non-Patent Citations (3)
| Title |
|---|
| AZUMA N et al, INTERNATIONAL DAIRY JOURNAL, ELSEVIER APPLIED SCIENCE, BARKING, GB, Vol. 16, No. 4, 1 April 2006, pages 370-378 * |
| BAYLESS K J et al. " Isolation and Biological Properties of Osteopontin from Bovine Milk ", PROTEIN EXPRESSION AND PURIFICATION, ACADEMIC PRESS, SAN DIEGO, CA, Vol. 9, No. 3, 1 April 1997, pages 309-314, * |
| SORENSEN S et al.: " Purification and characterization of osteopontin from human milk ", PROTEIN EXPRESSION AND PURIFICATION, ACADEMIC PRESS, SAN DIEGO, CA, Vol. 30, No. 2, 1 August 2003, pages 238-245 * |
Also Published As
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| AU2012222312B2 (en) | Method for isolating osteopontin using feeds containing cmp or casein species | |
| AU2003218623B2 (en) | A process of isolating lactoferrin | |
| NZ614872B2 (en) | Method for isolating osteopontin using feeds containing cmp or casein species | |
| NZ614874B2 (en) | Method for isolating osteopontin using concentrated feeds | |
| HK1217961B (zh) | 使用包含cmp或酪蛋白种类的给料分离骨桥蛋白的方法 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FGA | Letters patent sealed or granted (standard patent) |