KR101940829B1 - Cmp 또는 카제인 종을 함유하는 원료를 이용한 오스테오폰틴의 단리 방법 - Google Patents
Cmp 또는 카제인 종을 함유하는 원료를 이용한 오스테오폰틴의 단리 방법 Download PDFInfo
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- KR101940829B1 KR101940829B1 KR1020137026174A KR20137026174A KR101940829B1 KR 101940829 B1 KR101940829 B1 KR 101940829B1 KR 1020137026174 A KR1020137026174 A KR 1020137026174A KR 20137026174 A KR20137026174 A KR 20137026174A KR 101940829 B1 KR101940829 B1 KR 101940829B1
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- milk
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- derived raw
- derived
- protein
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- 238000000034 method Methods 0.000 title claims abstract description 83
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 title description 29
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Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/20—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from milk, e.g. casein; from whey
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/18—Ion-exchange chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Biochemistry (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Zoology (AREA)
- Analytical Chemistry (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Peptides Or Proteins (AREA)
- Dairy Products (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161448775P | 2011-03-03 | 2011-03-03 | |
| EP11156826.7 | 2011-03-03 | ||
| US61/448,775 | 2011-03-03 | ||
| EP11156826 | 2011-03-03 | ||
| PCT/EP2012/053748 WO2012117119A1 (en) | 2011-03-03 | 2012-03-05 | Method for isolating osteopontin using feeds containing cmp or casein species |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| KR20140029398A KR20140029398A (ko) | 2014-03-10 |
| KR101940829B1 true KR101940829B1 (ko) | 2019-01-21 |
Family
ID=44202290
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020137026174A Active KR101940829B1 (ko) | 2011-03-03 | 2012-03-05 | Cmp 또는 카제인 종을 함유하는 원료를 이용한 오스테오폰틴의 단리 방법 |
| KR1020137026175A Active KR101940830B1 (ko) | 2011-03-03 | 2012-03-05 | 농축된 원료를 이용한 오스테오폰틴의 단리 방법 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020137026175A Active KR101940830B1 (ko) | 2011-03-03 | 2012-03-05 | 농축된 원료를 이용한 오스테오폰틴의 단리 방법 |
Country Status (15)
| Country | Link |
|---|---|
| US (2) | US9181325B2 (enExample) |
| EP (2) | EP2681231B1 (enExample) |
| JP (2) | JP6012053B2 (enExample) |
| KR (2) | KR101940829B1 (enExample) |
| CN (3) | CN103476787B (enExample) |
| AR (2) | AR085620A1 (enExample) |
| AU (2) | AU2012222313B2 (enExample) |
| BR (2) | BR112013022251B1 (enExample) |
| CA (2) | CA2828669C (enExample) |
| DK (2) | DK2681230T3 (enExample) |
| EA (2) | EA023615B1 (enExample) |
| ES (2) | ES2565379T3 (enExample) |
| MX (2) | MX2013010019A (enExample) |
| PL (2) | PL2681230T3 (enExample) |
| WO (2) | WO2012117119A1 (enExample) |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2012149474A2 (en) * | 2011-04-28 | 2012-11-01 | Zoll Circulation, Inc. | Viral distribution of battery management parameters |
| EP2830647A1 (en) | 2012-03-28 | 2015-02-04 | Arla Foods amba | Nanoparticle aggregates containing osteopontin and calcium- and/or strontium-containing particles |
| US9956283B2 (en) * | 2013-07-05 | 2018-05-01 | Arla Foods Amba | Mammalian milk osteopontin for enhancing immune responsiveness |
| US9661868B2 (en) * | 2013-07-16 | 2017-05-30 | Mead Johnson Nutrition Company | Expanded bed adsorption methods for isolation of basic milk proteins including lactoferrin |
| KR200482960Y1 (ko) | 2016-04-07 | 2017-03-21 | 오숙희 | 고주파 얼굴 축소 시술기 |
| WO2018009647A1 (en) * | 2016-07-06 | 2018-01-11 | Building Block Nutritionals, Llc | Nutritional formula |
| EP3538053A1 (en) | 2016-11-08 | 2019-09-18 | Dentherapy Ltd | Compositions comprising fluoride and calcium and method for preparing them |
| CN111447842A (zh) * | 2017-12-22 | 2020-07-24 | 雀巢产品有限公司 | 提取物及其用途 |
| CN109613140B (zh) * | 2019-01-07 | 2021-10-08 | 雅士利国际集团有限公司 | 食品中酪蛋白磷酸肽的检测方法 |
| BR112022025567A2 (pt) | 2020-06-15 | 2023-03-07 | Building Block Nutritionals Llc | Fórmula nutricional |
| GB202010987D0 (en) | 2020-07-16 | 2020-09-02 | Dentherapy Ltd | Oral care compositions and methods |
| CN117377391A (zh) * | 2021-04-19 | 2024-01-09 | 雀巢产品有限公司 | 乳蛋白质级分作为骨桥蛋白源的用途 |
| CN113567573B (zh) * | 2021-07-09 | 2023-06-02 | 黑龙江飞鹤乳业有限公司 | 骨桥蛋白的分离方法和分析方法 |
| GB202110114D0 (en) | 2021-07-14 | 2021-08-25 | Dentherapy Ltd | Oral care compositions for use in treaatment |
| WO2024056840A1 (en) | 2022-09-16 | 2024-03-21 | Univerza V Ljubljani | Isolation of osteopontin and glycomacropeptide from whey |
| WO2025008636A1 (en) | 2023-07-06 | 2025-01-09 | Dentherapy Ltd | Oral care compositions for use in the treatment of dental health conditions |
| CN117837766A (zh) * | 2023-12-27 | 2024-04-09 | 美维仕(北京)健康管理有限公司 | 营养组合物及其应用 |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4615173B2 (ja) | 2000-01-07 | 2011-01-19 | アルラ・フーズ・エイ・エム・ビィ・エイ | ミルクからのオステオポンチンの単離のための方法 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU712430B2 (en) | 1996-10-01 | 1999-11-04 | Massey University | Process for isolating high purity glycomacropeptide from dairy products |
| WO1999033415A1 (en) | 1997-12-31 | 1999-07-08 | Depuy Orthopaedics, Inc. | Osteopontin-based compositions for enhancing bone repair |
| KR100960211B1 (ko) | 1998-05-06 | 2010-05-27 | 제넨테크, 인크. | 이온 교환 크로마토그래피에 의한 단백질 정제 방법 |
| GB2372949B (en) * | 1999-12-08 | 2003-08-20 | Univ Massey | Process for separation of whey proteins using a novel anion exchanger |
| NZ507335A (en) | 2000-10-05 | 2004-10-29 | New Zealand Dairy Board | Bone health compositions derived from milk comprising an acidic protein fraction but that does not contain CGMP |
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| Publication number | Priority date | Publication date | Assignee | Title |
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Non-Patent Citations (2)
| Title |
|---|
| International Dairy Journal.,16(4):370-378(2006.4.1.) |
| Protein Expression and Purification.,30(2):238-245(2003.8.1.) |
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