ES2559313T3 - Secuenciación de ácidos nucleicos de alto rendimiento por expansión - Google Patents

Secuenciación de ácidos nucleicos de alto rendimiento por expansión Download PDF

Info

Publication number
ES2559313T3
ES2559313T3 ES08771483.8T ES08771483T ES2559313T3 ES 2559313 T3 ES2559313 T3 ES 2559313T3 ES 08771483 T ES08771483 T ES 08771483T ES 2559313 T3 ES2559313 T3 ES 2559313T3
Authority
ES
Spain
Prior art keywords
anchor
subunit
residue
probe
link
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES08771483.8T
Other languages
English (en)
Spanish (es)
Inventor
Mark Stamatios. KOKORIS
Robert N. Mcruer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics Seattle Inc
Original Assignee
Stratos Genomics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Stratos Genomics Inc filed Critical Stratos Genomics Inc
Application granted granted Critical
Publication of ES2559313T3 publication Critical patent/ES2559313T3/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
ES08771483.8T 2007-06-19 2008-06-19 Secuenciación de ácidos nucleicos de alto rendimiento por expansión Active ES2559313T3 (es)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US305P 1995-06-19
US94503107P 2007-06-19 2007-06-19
US945031P 2007-06-19
US98191607P 2007-10-23 2007-10-23
US981916P 2007-10-23
US30507P 2007-10-25 2007-10-25
PCT/US2008/067507 WO2008157696A2 (en) 2007-06-19 2008-06-19 High throughput nucleic acid sequencing by expansion

Publications (1)

Publication Number Publication Date
ES2559313T3 true ES2559313T3 (es) 2016-02-11

Family

ID=39986452

Family Applications (2)

Application Number Title Priority Date Filing Date
ES08771483.8T Active ES2559313T3 (es) 2007-06-19 2008-06-19 Secuenciación de ácidos nucleicos de alto rendimiento por expansión
ES15164186T Active ES2959127T3 (es) 2007-06-19 2008-06-19 Secuenciación de ácidos nucleicos de alto rendimiento por expansión

Family Applications After (1)

Application Number Title Priority Date Filing Date
ES15164186T Active ES2959127T3 (es) 2007-06-19 2008-06-19 Secuenciación de ácidos nucleicos de alto rendimiento por expansión

Country Status (12)

Country Link
US (8) US7939259B2 (enExample)
EP (2) EP2171088B1 (enExample)
JP (1) JP5745842B2 (enExample)
KR (1) KR101685208B1 (enExample)
CN (1) CN102083998B (enExample)
AU (1) AU2008265691B2 (enExample)
CA (1) CA2691364C (enExample)
DK (1) DK2171088T3 (enExample)
ES (2) ES2559313T3 (enExample)
HK (1) HK1217736A1 (enExample)
IL (2) IL202821A (enExample)
WO (1) WO2008157696A2 (enExample)

Families Citing this family (215)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7311794B2 (en) 2004-05-28 2007-12-25 Wafergen, Inc. Methods of sealing micro wells
US20070190542A1 (en) * 2005-10-03 2007-08-16 Ling Xinsheng S Hybridization assisted nanopore sequencing
WO2007084126A1 (en) * 2006-01-19 2007-07-26 Allexcel, Inc. Solubilization and targeted delivery of drugs with self-assembling amphiphilic polymers
CN101495656B (zh) * 2006-06-07 2017-02-08 纽约哥伦比亚大学理事会 采用带修饰的核苷酸通过纳米通道进行dna序列测定
WO2009020682A2 (en) 2007-05-08 2009-02-12 The Trustees Of Boston University Chemical functionalization of solid-state nanopores and nanopore arrays and applications thereof
DK2171088T3 (en) * 2007-06-19 2016-01-25 Stratos Genomics Inc Nucleic acid sequencing in a high yield by expansion
US8278047B2 (en) * 2007-10-01 2012-10-02 Nabsys, Inc. Biopolymer sequencing by hybridization of probes to form ternary complexes and variable range alignment
CN101910410A (zh) * 2007-10-23 2010-12-08 斯特拉托斯基因公司 通过间隔进行高通量核酸测序
US9650668B2 (en) 2008-09-03 2017-05-16 Nabsys 2.0 Llc Use of longitudinally displaced nanoscale electrodes for voltage sensing of biomolecules and other analytes in fluidic channels
US8262879B2 (en) * 2008-09-03 2012-09-11 Nabsys, Inc. Devices and methods for determining the length of biopolymers and distances between probes bound thereto
CN102186989B (zh) * 2008-09-03 2021-06-29 纳伯塞斯2.0有限责任公司 用于流体通道中生物分子和其它分析物的电压感测的纵向移位纳米级电极的使用
US8583380B2 (en) 2008-09-05 2013-11-12 Aueon, Inc. Methods for stratifying and annotating cancer drug treatment options
EP2391726B1 (en) * 2009-01-29 2015-09-23 Stratos Genomics Inc. High throughput nucleic acid sequencing by expansion and related methods
EP2411536B1 (en) * 2009-03-27 2014-09-17 Nabsys, Inc. Methods for analyzing biomolecules and probes bound thereto
US8455260B2 (en) * 2009-03-27 2013-06-04 Massachusetts Institute Of Technology Tagged-fragment map assembly
WO2010114842A1 (en) 2009-03-30 2010-10-07 Ibis Biosciences, Inc. Bioagent detection systems, devices, and methods
AU2010242073C1 (en) 2009-04-30 2015-12-24 Good Start Genetics, Inc. Methods and compositions for evaluating genetic markers
US12129514B2 (en) 2009-04-30 2024-10-29 Molecular Loop Biosolutions, Llc Methods and compositions for evaluating genetic markers
US8246799B2 (en) * 2009-05-28 2012-08-21 Nabsys, Inc. Devices and methods for analyzing biomolecules and probes bound thereto
CA2808576A1 (en) * 2009-09-30 2011-04-07 Quantapore, Inc. Ultrafast sequencing of biological polymers using a labeled nanopore
US9678055B2 (en) 2010-02-08 2017-06-13 Genia Technologies, Inc. Methods for forming a nanopore in a lipid bilayer
US9605307B2 (en) 2010-02-08 2017-03-28 Genia Technologies, Inc. Systems and methods for forming a nanopore in a lipid bilayer
US8324914B2 (en) 2010-02-08 2012-12-04 Genia Technologies, Inc. Systems and methods for characterizing a molecule
WO2012003330A2 (en) 2010-06-30 2012-01-05 Stratos Genomics, Inc Multiplexed identification of nucleic acid sequences
CN103228798B (zh) 2010-09-24 2015-12-09 斯坦福大学托管董事会 使用固定的引物直接捕获、扩增及测序靶标dna
US8715933B2 (en) 2010-09-27 2014-05-06 Nabsys, Inc. Assay methods using nicking endonucleases
CN103270174B (zh) 2010-10-28 2016-08-24 生命技术公司 化学增强型引物组合物、方法和试剂盒
US8865404B2 (en) * 2010-11-05 2014-10-21 President And Fellows Of Harvard College Methods for sequencing nucleic acid molecules
US8859201B2 (en) 2010-11-16 2014-10-14 Nabsys, Inc. Methods for sequencing a biomolecule by detecting relative positions of hybridized probes
US8805095B2 (en) 2010-12-03 2014-08-12 International Business Machines Corporation Analysing character strings
US9121059B2 (en) 2010-12-22 2015-09-01 Genia Technologies, Inc. Nanopore-based single molecule characterization
US9163281B2 (en) 2010-12-23 2015-10-20 Good Start Genetics, Inc. Methods for maintaining the integrity and identification of a nucleic acid template in a multiplex sequencing reaction
EP2658988B1 (en) 2010-12-27 2018-01-24 Ibis Biosciences, Inc. Nucleic acid sample preparation methods and compositions
US9581563B2 (en) 2011-01-24 2017-02-28 Genia Technologies, Inc. System for communicating information from an array of sensors
US9110478B2 (en) 2011-01-27 2015-08-18 Genia Technologies, Inc. Temperature regulation of measurement arrays
US11274341B2 (en) 2011-02-11 2022-03-15 NABsys, 2.0 LLC Assay methods using DNA binding proteins
WO2013022778A1 (en) 2011-08-05 2013-02-14 Ibis Biosciences, Inc. Nucleic acid sequencing by electrochemical detection
JP6457811B2 (ja) 2011-09-23 2019-01-23 オックスフォード ナノポール テクノロジーズ リミテッド ポリマー単位を含むポリマーの解析
EP3604555B1 (en) 2011-10-14 2024-12-25 President and Fellows of Harvard College Sequencing by structure assembly
EP2794928B1 (en) 2011-12-22 2019-02-20 President and Fellows of Harvard College Compositions and methods for analyte detection
WO2013096819A2 (en) 2011-12-22 2013-06-27 Ibis Biosciences, Inc. Macromolecule positioning by electrical potential
ES2991004T3 (es) 2011-12-22 2024-12-02 Harvard College Métodos para la detección de analitos
WO2013096799A1 (en) 2011-12-22 2013-06-27 Ibis Biosciences, Inc. Systems and methods for isolating nucleic acids from cellular samples
WO2013096838A2 (en) 2011-12-22 2013-06-27 Ibis Biosciences, Inc. Systems and methods for isolating nucleic acids
WO2014163886A1 (en) 2013-03-12 2014-10-09 President And Fellows Of Harvard College Method of generating a three-dimensional nucleic acid containing matrix
US9970061B2 (en) 2011-12-27 2018-05-15 Ibis Biosciences, Inc. Bioagent detection oligonucleotides
WO2013102091A1 (en) 2011-12-28 2013-07-04 Ibis Biosciences, Inc. Nucleic acid ligation systems and methods
WO2013102081A2 (en) 2011-12-29 2013-07-04 Ibis Biosciences, Inc. Macromolecule delivery to nanowells
WO2013101743A2 (en) 2011-12-30 2013-07-04 Abbott Molecular, Inc. Microorganism nucelic acid purification from host samples
WO2013104990A1 (en) 2012-01-09 2013-07-18 Oslo Universitetssykehus Hf Methods and biomarkers for analysis of colorectal cancer
EP2802666B1 (en) 2012-01-13 2018-09-19 Data2Bio Genotyping by next-generation sequencing
US8986629B2 (en) 2012-02-27 2015-03-24 Genia Technologies, Inc. Sensor circuit for controlling, detecting, and measuring a molecular complex
WO2013152114A1 (en) 2012-04-03 2013-10-10 The Regents Of The University Of Michigan Biomarker associated with irritable bowel syndrome and crohn's disease
US8209130B1 (en) 2012-04-04 2012-06-26 Good Start Genetics, Inc. Sequence assembly
US10227635B2 (en) 2012-04-16 2019-03-12 Molecular Loop Biosolutions, Llc Capture reactions
WO2013166302A1 (en) 2012-05-02 2013-11-07 Ibis Biosciences, Inc. Nucleic acid sequencing systems and methods
ES2683707T3 (es) 2012-05-02 2018-09-27 Ibis Biosciences, Inc. Secuenciación de ADN
WO2013184754A2 (en) 2012-06-05 2013-12-12 President And Fellows Of Harvard College Spatial sequencing of nucleic acids using dna origami probes
WO2013188841A1 (en) 2012-06-15 2013-12-19 Genia Technologies, Inc. Chip set-up and high-accuracy nucleic acid sequencing
WO2014005076A2 (en) 2012-06-29 2014-01-03 The Regents Of The University Of Michigan Methods and biomarkers for detection of kidney disorders
CN108875312A (zh) 2012-07-19 2018-11-23 哈佛大学校长及研究员协会 利用核酸存储信息的方法
CA2887665A1 (en) 2012-10-16 2014-04-24 Abbott Molecular Inc. Methods and apparatus to sequence a nucleic acid
US9651539B2 (en) 2012-10-28 2017-05-16 Quantapore, Inc. Reducing background fluorescence in MEMS materials by low energy ion beam treatment
CA2890515C (en) 2012-11-09 2021-11-09 Stratos Genomics, Inc. Concentrating a target molecule for sensing by a nanopore
US9605309B2 (en) 2012-11-09 2017-03-28 Genia Technologies, Inc. Nucleic acid sequencing using tags
GB201222928D0 (en) 2012-12-19 2013-01-30 Oxford Nanopore Tech Ltd Analysis of a polynucleotide
US9914966B1 (en) 2012-12-20 2018-03-13 Nabsys 2.0 Llc Apparatus and methods for analysis of biomolecules using high frequency alternating current excitation
WO2014112950A1 (en) * 2013-01-18 2014-07-24 Nanyang Technological University Method of preparing a keratin-based biomaterial and keratin-based biomaterial formed thereof
EP2956550B1 (en) 2013-01-18 2020-04-08 Nabsys 2.0 LLC Enhanced probe binding
US9759711B2 (en) 2013-02-05 2017-09-12 Genia Technologies, Inc. Nanopore arrays
US20140287946A1 (en) 2013-03-14 2014-09-25 Ibis Biosciences, Inc. Nucleic acid control panels
EP2971171A4 (en) 2013-03-14 2016-11-02 Abbott Molecular Inc SYSTEMS AND METHODS FOR MULTIPLEX AMPLIFICATION SPECIFIC TO METHYLATION
CA2905410A1 (en) 2013-03-15 2014-09-25 Abbott Molecular Inc. Systems and methods for detection of genomic copy number changes
ES2716094T3 (es) 2013-03-15 2019-06-10 Ibis Biosciences Inc Métodos para analizar la contaminación en la secuenciación del ADN
JP6399610B2 (ja) * 2013-05-24 2018-10-10 クアンタポール， インコーポレイテッド 混合ｆｒｅｔ検出を用いたナノポアに基づく核酸の分析
MY197877A (en) 2013-06-04 2023-07-22 Harvard College Rna-guided transcriptional regulation
US20150037787A1 (en) * 2013-07-31 2015-02-05 International Business Machines Corporation Polynucleotide configuration for reliable electrical and optical sensing
ES2764096T3 (es) 2013-08-19 2020-06-02 Abbott Molecular Inc Bibliotecas de secuenciación de próxima generación
US9551697B2 (en) 2013-10-17 2017-01-24 Genia Technologies, Inc. Non-faradaic, capacitively coupled measurement in a nanopore cell array
WO2015057635A1 (en) 2013-10-18 2015-04-23 The Regents Of The University Of Michigan Systems and methods for determining a treatment course of action
US10851414B2 (en) 2013-10-18 2020-12-01 Good Start Genetics, Inc. Methods for determining carrier status
US9567630B2 (en) 2013-10-23 2017-02-14 Genia Technologies, Inc. Methods for forming lipid bilayers on biochips
JP6461943B2 (ja) 2013-10-23 2019-01-30 ジェニア・テクノロジーズ・インコーポレイテッド ナノポアを備えた高速分子検知
US10768181B2 (en) 2013-12-17 2020-09-08 The Brigham And Women's Hospital, Inc. Detection of an antibody against a pathogen
WO2015107430A2 (en) 2014-01-16 2015-07-23 Oslo Universitetssykehus Hf Methods and biomarkers for detection and prognosis of cervical cancer
FR3020071B1 (fr) 2014-04-17 2017-12-22 Dna Script Procede de synthese d'acides nucleiques, notamment d'acides nucleiques de grande longueur, utilisation du procede et kit pour la mise en œuvre du procede
WO2015164602A2 (en) * 2014-04-23 2015-10-29 Children's Medical Center Corporation High-throughput structure determination using nucleic acid calipers
US10934581B2 (en) 2014-04-30 2021-03-02 International Business Machines Corporation Bow tie DNA compositions and methods
US20150315637A1 (en) * 2014-04-30 2015-11-05 International Business Machines Corporation Bow tie dna compositions and methods
EP3143158A1 (en) 2014-05-14 2017-03-22 Stratos Genomics, Inc. Translocation control for sensing by a nanopore
WO2015188178A1 (en) 2014-06-06 2015-12-10 The Regents Of The University Of Michigan Compositions and methods for characterizing and diagnosing periodontal disease
EP3161157B1 (en) 2014-06-24 2024-03-27 Bio-Rad Laboratories, Inc. Digital pcr barcoding
EP3161490B1 (en) 2014-06-27 2019-10-02 Abbott Laboratories Compositions and methods for detecting human pegivirus 2 (hpgv-2)
WO2016007839A1 (en) 2014-07-11 2016-01-14 President And Fellows Of Harvard College Methods for high-throughput labelling and detection of biological features in situ using microscopy
RU2697502C2 (ru) 2014-07-24 2019-08-14 Эбботт Молекьюлар Инк. Композиции и способы обнаружения и анализа микобактерии туберкулеза (mycobacterium tuberculosis)
FR3025201B1 (fr) 2014-09-02 2018-10-12 Dna Script Nucleotides modifies pour la synthese d'acides nucleiques, un kit renfermant de tels nucleotides et leur utilisation pour la production de genes ou sequences d'acides nucleiques synthetiques
US11408024B2 (en) 2014-09-10 2022-08-09 Molecular Loop Biosciences, Inc. Methods for selectively suppressing non-target sequences
KR20170064540A (ko) * 2014-09-26 2017-06-09 투 포어 가이즈, 인코포레이티드 합성 프로브의 나노포어 감지에 의한 표적 서열 검출
WO2016053883A1 (en) * 2014-10-03 2016-04-07 Life Technologies Corporation Detection, identification, validation and enrichment of target nucleic acids
WO2016057829A1 (en) 2014-10-10 2016-04-14 Quantapore, Inc. Nanopore-based polymer analysis with mutually-quenching fluorescent labels
EP4397972A3 (en) 2014-10-16 2024-10-09 Oxford Nanopore Technologies PLC Alignment mapping estimation
US9624537B2 (en) 2014-10-24 2017-04-18 Quantapore, Inc. Efficient optical analysis of polymers using arrays of nanostructures
HK1244813A1 (zh) * 2014-11-20 2018-08-17 F. Hoffmann-La Roche Ag 氰脲酸酯磷醯胺酯及其衍生物，其用途和合成
CN107077538B (zh) * 2014-12-10 2020-08-07 深圳华大生命科学研究院 测序数据处理装置和方法
CN107077533B (zh) * 2014-12-10 2021-07-27 深圳华大生命科学研究院 测序数据处理装置和方法
CN107075564A (zh) * 2014-12-10 2017-08-18 深圳华大基因研究院 确定肿瘤核酸浓度的方法和装置
WO2016112073A1 (en) * 2015-01-06 2016-07-14 Good Start Genetics, Inc. Screening for structural variants
US10208339B2 (en) 2015-02-19 2019-02-19 Takara Bio Usa, Inc. Systems and methods for whole genome amplification
DK3259602T3 (da) 2015-02-20 2021-02-15 Takara Bio Usa Inc Fremgangsmåde til hurtig nøjagtig dispensering, visualisering og analyse af enkeltceller
US9928869B2 (en) 2015-07-13 2018-03-27 President And Fellows Of Harvard College Methods for retrievable information storage using nucleic acids
WO2017011565A1 (en) 2015-07-14 2017-01-19 Abbott Molecular Inc. Compositions and methods for identifying drug resistant tuberculosis
CA2992449A1 (en) 2015-07-14 2017-01-19 Abbott Molecular Inc. Purification of nucleic acids using copper-titanium oxides
CA2994160C (en) 2015-07-30 2021-08-10 Massachusetts Eye And Ear Infirmary Ancestral virus sequences and uses thereof
EP3371329B1 (en) 2015-11-03 2026-03-25 President and Fellows of Harvard College Method and apparatus for volumetric imaging of a three-dimensional nucleic acid containing matrix
JP7064439B2 (ja) * 2015-11-04 2022-05-10 アトレカ インコーポレイテッド 単一細胞に関連する核酸の解析のための、核酸バーコードの組み合わせセット
EP3904528A1 (en) 2015-11-16 2021-11-03 Stratos Genomics Inc. Dp04 polymerase variants
US10730030B2 (en) 2016-01-08 2020-08-04 Bio-Rad Laboratories, Inc. Multiple beads per droplet resolution
US10626390B2 (en) 2016-02-08 2020-04-21 RGENE, Inc. Multiple ligase compositions, systems, and methods
EP3414347A4 (en) * 2016-02-11 2019-10-09 Qiagen Sciences, LLC ADDITIVE FOR IMPROVING SEQUENCING THROUGH SYNTHETIC PERFORMANCE
WO2017160788A2 (en) 2016-03-14 2017-09-21 RGENE, Inc. HYPER-THERMOSTABLE LYSINE-MUTANT ssDNA/RNA LIGASES
US11486873B2 (en) 2016-03-31 2022-11-01 Ontera Inc. Multipore determination of fractional abundance of polynucleotide sequences in a sample
CA3210120C (en) 2016-04-25 2024-04-09 President And Fellows Of Harvard College Hybridization chain reaction methods for in situ molecular detection
US10823721B2 (en) 2016-07-05 2020-11-03 Quantapore, Inc. Optically based nanopore sequencing
WO2018013509A1 (en) 2016-07-11 2018-01-18 Arizona Board Of Regents On Behalf Of The University Of Arizona Compositions and methods for diagnosing and treating arrhythmias
WO2018013999A1 (en) 2016-07-15 2018-01-18 Am Chemicals Llc Non-nucleosidic solid supports and phosphoramidite building blocks for oligonucleotide synthesis
CA3020629A1 (en) 2016-07-21 2018-01-25 Takara Bio Usa, Inc. Multi-z imaging and dispensing with multi-well devices
US11543417B2 (en) 2016-08-29 2023-01-03 Oslo Universitetssykehus Hf ChIP-seq assays
JP7239465B2 (ja) 2016-08-31 2023-03-14 プレジデント アンド フェローズ オブ ハーバード カレッジ 蛍光ｉｎ ｓｉｔｕ配列決定による検出のための核酸配列ライブラリの作製法
GB2569252A (en) 2016-08-31 2019-06-12 Harvard College Methods of combining the detection of biomolecules into a single assay using fluorescent in situ sequencing
US12116570B2 (en) 2016-09-29 2024-10-15 Roche Sequencing Solutions, Inc. Directed evolution of enzymes by plasmid tagging in droplets
EP3526349B1 (en) 2016-10-13 2021-03-17 F. Hoffmann-La Roche AG Molecular detection and counting using nanopores
US11021738B2 (en) 2016-12-19 2021-06-01 Bio-Rad Laboratories, Inc. Droplet tagging contiguity preserved tagmented DNA
ES2987024T3 (es) 2017-05-04 2024-11-13 Hoffmann La Roche Variantes de polimerasa DP04
EP3634992B1 (en) 2017-06-08 2024-03-27 The Brigham And Women's Hospital, Inc. Methods and compositions for identifying epitopes
EP3642363B1 (en) 2017-06-20 2025-12-24 Bio-Rad Laboratories, Inc. Mda using bead oligonucleotide
EP3665274B1 (en) 2017-08-07 2025-10-15 DNA Script Variants of family a dna polymerase and uses thereof
CN118186055A (zh) 2017-11-02 2024-06-14 生物辐射实验室股份有限公司 基于转座酶的基因组分析
US11131646B2 (en) 2017-11-03 2021-09-28 Robert Bosch Gmbh Electrochemical sequencing of DNA using an edge electrode
US11530392B2 (en) 2017-12-11 2022-12-20 Stratos Genomics, Inc. DPO4 polymerase variants with improved accuracy
JP7074857B2 (ja) * 2017-12-21 2022-05-24 エフ．ホフマン－ラ ロシュ アーゲー 一方向性の核酸配列決定のための組成物及び方法
JP7275148B2 (ja) 2018-01-05 2023-05-17 ストラトス ゲノミクス インコーポレイテッド 副溝結合成分による核酸重合の強化
CN111771001B (zh) * 2018-01-05 2024-09-06 F·霍夫曼-罗氏股份公司 通过芳香族化合物增强核酸聚合
US10752887B2 (en) 2018-01-08 2020-08-25 Dna Script Variants of terminal deoxynucleotidyl transferase and uses thereof
WO2019135007A1 (en) 2018-01-08 2019-07-11 Dna Script Variants of terminal deoxynucleotidyl transferase and uses thereof
JP6559814B2 (ja) * 2018-01-26 2019-08-14 株式会社日立ハイテクノロジーズ 生体分子測定方法
WO2019152395A1 (en) 2018-01-31 2019-08-08 Bio-Rad Laboratories, Inc. Methods and compositions for deconvoluting partition barcodes
US12037707B2 (en) 2018-04-05 2024-07-16 Massachusetts Eye And Ear Infirmary Methods of making and using combinatorial barcoded nucleic acid libraries having defined variation
US11512002B2 (en) 2018-04-18 2022-11-29 University Of Virginia Patent Foundation Silica materials and methods of making thereof
EP3788377A1 (en) 2018-05-04 2021-03-10 Abbott Laboratories Hbv diagnostic, prognostic, and therapeutic methods and products
EP3826762A1 (en) 2018-07-23 2021-06-02 DNA Script Massively parallel enzymatic synthesis of nucleic acid strands
WO2020028194A1 (en) 2018-07-30 2020-02-06 Readcoor, Inc. Methods and systems for sample processing or analysis
EP4249651B1 (en) 2018-08-20 2025-01-29 Bio-Rad Laboratories, Inc. Nucleotide sequence generation by barcode bead-colocalization in partitions
WO2020076976A1 (en) 2018-10-10 2020-04-16 Readcoor, Inc. Three-dimensional spatial molecular indexing
WO2020102513A1 (en) 2018-11-14 2020-05-22 Arizona Board Of Regents On Behalf Of The University Of Arizona Systems and methods for characterizing and treating cancer
CN113423841A (zh) 2018-12-13 2021-09-21 Dna斯克瑞普特公司 细胞和生物分子上的直接寡核苷酸合成
EP3924500A1 (en) 2019-02-12 2021-12-22 DNA Script Efficient product cleavage in template-free enzymatic synthesis of polynucleotides
JP2022523362A (ja) * 2019-02-21 2022-04-22 ストラトス ゲノミクス インコーポレイテッド 単一分子配列決定における使用のための拡張可能ポリマーの固体合成のための方法、組成物、およびデバイス
WO2020236526A1 (en) 2019-05-23 2020-11-26 Stratos Genomics, Inc. Translocation control elements, reporter codes, and further means for translocation control for use in nanopore sequencing
WO2020247950A1 (en) 2019-06-07 2020-12-10 Bio-Rad Laboratories, Inc. Multiple beads per droplet resolution
WO2020254672A1 (en) 2019-06-19 2020-12-24 Therycell Gmbh Spatial characterisation of target structures in a sample
US11755922B2 (en) * 2019-10-04 2023-09-12 The Board Of Trustees Of The University Of Illinois On-chip nanoscale storage system using chimeric DNA
EP4051778A4 (en) 2019-10-30 2023-11-22 Stratos Genomics Inc. METHODS AND COMPOSITIONS FOR ASSEMBLING BIOLOGICAL NANOPORES
JP7344786B2 (ja) 2019-12-19 2023-09-14 株式会社日立製作所 溶液中の任意のｄｎａ配列を同定する方法
WO2021155063A1 (en) 2020-01-29 2021-08-05 Readcoor, Llc Compositions and methods for analyte detection
WO2021152586A1 (en) 2020-01-30 2021-08-05 Yeda Research And Development Co. Ltd. Methods of analyzing microbiome, immunoglobulin profile and physiological state
WO2021214766A1 (en) 2020-04-21 2021-10-28 Yeda Research And Development Co. Ltd. Methods of diagnosing viral infections and vaccines thereto
WO2021224677A1 (en) 2020-05-05 2021-11-11 Akershus Universitetssykehus Hf Compositions and methods for characterizing bowel cancer
JP7706479B2 (ja) 2020-06-10 2025-07-11 エフ． ホフマン－ラ ロシュ アーゲー 膜における自己制限性プロテイン細孔挿入のためのファラデーシステム及び方法
WO2022020359A1 (en) 2020-07-24 2022-01-27 The Regents Of The University Of Michigan Compositions and methods for detecting and treating high grade subtypes of uterine cancer
US20220049303A1 (en) 2020-08-17 2022-02-17 Readcoor, Llc Methods and systems for spatial mapping of genetic variants
CN112201305B (zh) * 2020-10-12 2021-12-07 冬青南京生物科技有限公司 一种单细胞核中基因数目测定设备
GB202103605D0 (en) 2021-03-16 2021-04-28 Oxford Nanopore Tech Ltd Alignment of target and reference sequences of polymer units
EP4314810B1 (en) 2021-03-31 2026-02-18 Illumina, Inc. Nanopore sensor devices and method
US20230014008A1 (en) * 2021-07-13 2023-01-19 10X Genomics, Inc. Methods for improving spatial performance
CN118266034A (zh) 2021-10-04 2024-06-28 F·霍夫曼-罗氏股份公司 在线碱基识别压缩
WO2023081031A1 (en) 2021-11-08 2023-05-11 Illumina, Inc. Identifying nucleotides using changes in impedance between electrodes
WO2023186842A1 (en) 2022-03-28 2023-10-05 F. Hoffmann-La Roche Ag Synthesis of isomerically pure polyol-based phosphoramidites
EP4499278A1 (en) 2022-03-31 2025-02-05 Illumina, Inc. Barriers including biological nanopore for dna sequencing, the barriers being made of co-polymers with end and/or middle groups, and methods of making the same
CN119013093A (zh) 2022-03-31 2024-11-22 伊鲁米纳公司 包括使用二嵌段或三嵌段共聚物的屏障的纳米孔装置以及制造该纳米孔装置的方法
US20250205655A1 (en) 2022-03-31 2025-06-26 Illumina, Inc. Amphiphilic polymers to be used in barriers and preparation thereof, barriers with nanopores and preparation thereof
WO2023187106A1 (en) 2022-03-31 2023-10-05 Illumina Cambridge Limited Barriers including cross-linked amphiphilic molecules, and methods of making the same
WO2023187001A1 (en) 2022-03-31 2023-10-05 Illumina Cambridge Limited Devices including osmotically balanced barriers, and methods of making and using the same
WO2023187112A1 (en) 2022-03-31 2023-10-05 Illumina Cambridge Limited Amphiphilic block-co-polymers cross-linked with different cross-linkers consecutively forming a cross-linking hierarchy
WO2023187081A1 (en) 2022-03-31 2023-10-05 Illumina Cambridge Limited Methods for inserting nanopores into polymeric membranes using chaotropic solvents
CN119137286A (zh) 2022-05-02 2024-12-13 豪夫迈·罗氏有限公司 在纳米孔测序期间减少普鲁士蓝形成的组合物和方法
WO2024074412A1 (en) 2022-10-03 2024-04-11 F. Hoffmann-La Roche Ag Single molecule multi-molecular trace methods and systems
WO2024083982A1 (en) 2022-10-21 2024-04-25 F. Hoffmann-La Roche Ag Detection of modified nucleobases in nucleic acid samples
EP4649168A1 (en) 2023-01-13 2025-11-19 F. Hoffmann-La Roche AG Detection of modified nucleobases in dna samples
WO2024200192A1 (en) 2023-03-30 2024-10-03 F. Hoffmann-La Roche Ag Modulation of target molecule-lipid bilayer interactions
EP4705012A1 (en) 2023-05-04 2026-03-11 F. Hoffmann-La Roche AG Hybrid triblock copolymer membrane compositions and methods for nanopore sequencing
CN121057766A (zh) 2023-05-04 2025-12-02 豪夫迈·罗氏有限公司 经修饰的三嵌段共聚物化合物及其使用方法
WO2024242847A1 (en) 2023-05-25 2024-11-28 Illumina, Inc. Methods of making barriers including nanopores and cross¬ linked amphiphilic molecules, and barriers formed using same
WO2025082960A1 (en) 2023-10-18 2025-04-24 F. Hoffmann-La Roche Ag Dpo4 polymerase variants with improved properties
WO2025106652A1 (en) 2023-11-17 2025-05-22 Illumina, Inc. Fluidic devices, nanopore instruments, and methods
WO2025132780A2 (en) 2023-12-22 2025-06-26 F. Hoffmann-La Roche Ag Methods and compositions for nucleic acid library and template preparation for duplexed sequencing by expansion
WO2025132779A2 (en) 2023-12-22 2025-06-26 F. Hoffmann-La Roche Ag Methods and compositions for nucleic acid library and template preparation for duplexed sequencing by expansion
WO2025137293A2 (en) 2023-12-22 2025-06-26 Roche Sequencing Solutions, Inc. Enhancement of nucleic acid polymerization by aromatic compounds
WO2025149478A1 (en) 2024-01-12 2025-07-17 F. Hoffmann-La Roche Ag Compositions of modified nucleoside triphosphates
WO2025149479A1 (en) 2024-01-12 2025-07-17 Roche Diagnostics Gmbh Synthesis of modified nucleoside triphosphates
WO2025174660A1 (en) 2024-02-12 2025-08-21 Illumina, Inc. Recharging for nanopore system
WO2025174659A1 (en) 2024-02-12 2025-08-21 Illumina, Inc. Net-zero current scheme for nanopore system
EP4603180A1 (en) 2024-02-15 2025-08-20 Roche Diagnostics International AG Techniques for synthesizing a macromolecule from a sample
WO2025212586A1 (en) 2024-04-02 2025-10-09 Roche Sequencing Solutions, Inc. High throughput inramolecular consensus reads
WO2025240460A2 (en) 2024-05-14 2025-11-20 Roche Molecular Systems, Inc. Assay for detection of mutations conferring resistance to treatment with an immunotherapeutic agent
WO2026033006A1 (en) 2024-08-07 2026-02-12 Roche Sequencing Solutions, Inc. Nickase-mediated linear amplification of library constructs for duplex sequencing
WO2026033007A1 (en) 2024-08-07 2026-02-12 F. Hoffmann-La Roche Ag Automated solid-state amplification of library constructs for duplex sequencing
WO2026046950A1 (en) 2024-08-28 2026-03-05 F. Hoffmann-La Roche Ag Methods and compositions for size-selective elution of xpandomer macromolecules for nanopore sequencing
WO2026050541A1 (en) 2024-08-28 2026-03-05 Roche Sequencing Solutions, Inc. Methods and systems for allele-specific copy number calling
WO2026050290A1 (en) 2024-08-29 2026-03-05 Roche Sequencing Solutions, Inc. Lipid composition and methods of use in a nanopore sequencing assay
WO2026050292A1 (en) 2024-08-29 2026-03-05 Roche Sequencing Solutions, Inc. Optimized reagent introduction during a nanopore sequencing assay
WO2026050289A1 (en) 2024-08-29 2026-03-05 Roche Sequencing Solutions, Inc. Pre-and post-sequencing cleaning protocols for nanopore sequencing
WO2026050150A1 (en) 2024-08-30 2026-03-05 Roche Sequencing Solutions, Inc. Dynamic control of spatial and temporal variability in nanopore sequencing
WO2026050121A1 (en) 2024-08-30 2026-03-05 Roche Sequencing Solutions, Inc. Compositions for replicating a nucleic acid template
WO2026050702A1 (en) 2024-08-31 2026-03-05 Roche Sequencing Solutions, Inc. Systems and methods for tuning sequencing parameters
CN118904278A (zh) * 2024-10-08 2024-11-08 祥符实验室 一种核酸固相合成提取磁珠及其制备方法和应用

Family Cites Families (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4458066A (en) 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
US4415732A (en) 1981-03-27 1983-11-15 University Patents, Inc. Phosphoramidite compounds and processes
US5378825A (en) 1990-07-27 1995-01-03 Isis Pharmaceuticals, Inc. Backbone modified oligonucleotide analogs
EP0544824B1 (en) 1990-07-27 1997-06-11 Isis Pharmaceuticals, Inc. Nuclease resistant, pyrimidine modified oligonucleotides that detect and modulate gene expression
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5432272A (en) 1990-10-09 1995-07-11 Benner; Steven A. Method for incorporating into a DNA or RNA oligonucleotide using nucleotides bearing heterocyclic bases
AU3222793A (en) 1991-11-26 1993-06-28 Gilead Sciences, Inc. Enhanced triple-helix and double-helix formation with oligomers containing modified pyrimidines
FR2702874B1 (fr) 1993-03-18 1995-06-09 Centre Nat Rech Scient Cellule memoire insensible aux rayonnements.
DK0691980T3 (da) 1993-03-30 1997-12-29 Sanofi Sa 7-deazapurinmodifiderende oligonukleotider
AU6632094A (en) 1993-04-19 1994-11-08 Gilead Sciences, Inc. Enhanced triple-helix and double-helix formation with oligomers containing modified purines
FR2705099B1 (fr) 1993-05-12 1995-08-04 Centre Nat Rech Scient Oligonucléotides phosphorothioates triesters et procédé de préparation.
DE4438918A1 (de) 1994-11-04 1996-05-09 Hoechst Ag Modifizierte Oligonukleotide, deren Herstellung sowie deren Verwendung
US6150510A (en) 1995-11-06 2000-11-21 Aventis Pharma Deutschland Gmbh Modified oligonucleotides, their preparation and their use
WO1998023733A2 (en) 1996-11-27 1998-06-04 University Of Washington Thermostable polymerases having altered fidelity
US7399844B2 (en) * 1998-07-09 2008-07-15 Agilent Technologies, Inc. Method and reagents for analyzing the nucleotide sequence of nucleic acids
US6242589B1 (en) 1998-07-14 2001-06-05 Isis Pharmaceuticals, Inc. Phosphorothioate oligonucleotides having modified internucleoside linkages
AU2206800A (en) 1998-12-11 2000-06-26 Regents Of The University Of California, The Targeted molecular bar codes and methods for using the same
NO986133D0 (no) * 1998-12-23 1998-12-23 Preben Lexow FremgangsmÕte for DNA-sekvensering
US7371851B1 (en) 1999-03-18 2008-05-13 Complete Genomics As Methods of cloning and producing fragment chains with readable information content
AU5763000A (en) * 1999-06-22 2001-01-09 President And Fellows Of Harvard College Molecular and atomic scale evaluation of biopolymers
EP1072679A3 (en) 1999-07-20 2002-07-31 Agilent Technologies, Inc. (a Delaware corporation) Method of producing nucleic acid molecules with reduced secondary structure
KR20020047136A (ko) 1999-09-01 2002-06-21 누에이스 테크놀로지스 리미티드 올리고뉴클레오타이드 트리포스페이트 빌딩 블록을 이용한주형-의존 핵산 중합
US6329178B1 (en) 2000-01-14 2001-12-11 University Of Washington DNA polymerase mutant having one or more mutations in the active site
US6951720B2 (en) 2001-05-10 2005-10-04 San Diego State University Foundation Use of phosphorothiolate polynucleotides in ligating nucleic acids
GB0308852D0 (en) 2003-04-16 2003-05-21 Lingvitae As Method
US20070048748A1 (en) 2004-09-24 2007-03-01 Li-Cor, Inc. Mutant polymerases for sequencing and genotyping
WO2006076650A2 (en) * 2005-01-12 2006-07-20 Applera Corporation Compositions, methods, and kits for selective amplification of nucleic acids
CN101189345A (zh) * 2005-02-01 2008-05-28 Ab先进基因分析公司 珠基测序的试剂、方法和文库
EP2230315A1 (en) * 2005-02-01 2010-09-22 AB Advanced Genetic Analysis Corporation Nucleic acid sequencing by performing successive cycles of duplex extension
DK2171088T3 (en) * 2007-06-19 2016-01-25 Stratos Genomics Inc Nucleic acid sequencing in a high yield by expansion
CN201189345Y (zh) * 2008-05-23 2009-02-04 北京航天长峰股份有限公司 一种氧浓度调节装置

Also Published As

Publication number Publication date
CA2691364A1 (en) 2008-12-24
EP2171088A2 (en) 2010-04-07
AU2008265691A1 (en) 2008-12-24
US7939259B2 (en) 2011-05-10
WO2008157696A2 (en) 2008-12-24
US20180334729A1 (en) 2018-11-22
US20250109449A1 (en) 2025-04-03
IL202821A (en) 2017-07-31
HK1217736A1 (en) 2017-01-20
AU2008265691B2 (en) 2014-04-24
KR101685208B1 (ko) 2016-12-09
US20110251079A1 (en) 2011-10-13
HK1143188A1 (zh) 2010-12-24
JP2010530759A (ja) 2010-09-16
ES2959127T3 (es) 2024-02-20
US20110237787A1 (en) 2011-09-29
KR20100044790A (ko) 2010-04-30
EP2171088B1 (en) 2015-10-14
CN102083998B (zh) 2016-08-03
US20220064741A1 (en) 2022-03-03
EP2952587A1 (en) 2015-12-09
IL224295A (en) 2017-09-28
US20130172197A1 (en) 2013-07-04
DK2171088T3 (en) 2016-01-25
US20090035777A1 (en) 2009-02-05
JP5745842B2 (ja) 2015-07-08
EP2952587B1 (en) 2023-07-05
US8349565B2 (en) 2013-01-08
US8324360B2 (en) 2012-12-04
CN102083998A (zh) 2011-06-01
CA2691364C (en) 2020-06-16
US9920386B2 (en) 2018-03-20
WO2008157696A3 (en) 2009-04-02
US20160208344A1 (en) 2016-07-21

Similar Documents

Publication Publication Date Title
ES2559313T3 (es) Secuenciación de ácidos nucleicos de alto rendimiento por expansión
US8592182B2 (en) High throughput nucleic acid sequencing by spacing
EP2391726B1 (en) High throughput nucleic acid sequencing by expansion and related methods
ES2887177T3 (es) Método de preparación de una biblioteca de secuenciación de ácidos nucleicos
HK1143188B (en) High throughput nucleic acid sequencing by expansion