ES2539970T3 - Bacteria genéticamente modificada de la especie Listeria monocytogenes - Google Patents

Bacteria genéticamente modificada de la especie Listeria monocytogenes Download PDF

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Publication number
ES2539970T3
ES2539970T3 ES11716841.9T ES11716841T ES2539970T3 ES 2539970 T3 ES2539970 T3 ES 2539970T3 ES 11716841 T ES11716841 T ES 11716841T ES 2539970 T3 ES2539970 T3 ES 2539970T3
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Prior art keywords
genetically modified
listeria monocytogenes
modified bacterium
species listeria
species
Prior art date
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ES11716841.9T
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English (en)
Inventor
Peter Rossmanith
Karin Fruehwirth
Martin Wagner
Sabine Fuchs
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Merck Patent GmbH
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Merck Patent GmbH
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Publication date
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/166—Oligonucleotides used as internal standards, controls or normalisation probes

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Una bacteria genéticamente modificada de la especie Listeria monocytogenes, en la que se ha delecionado el locus genómico del factor de transcripción PrfA, caracterizada porque a nivel genómico contiene una secuencia artificial que actúa como control interno de amplificación (IAC).

Description

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E11716841
18-06-2015
TABLA 1. Secuencias de oligonucleótidos y cebadores y organismos
Oligonucleótidos
Secuencia / Marcado Ref. / Fuente
Lip 1
Cebador sentidoa 5´-GAT ACA GAA ACA TCG GTT GGC-3´ D´Agostino et al., 2004
Lip 2
Cebador complementarioa 5´-GTG TAA TCT TGA TGC CAT CAG G-3´ D´Agostino et al., 2004
Lip Bam
Cebador sentidob 5`GCG CGG ATC CGA TAC AGA AAC ATC GGT TGG C´3 SEQ ID NO: 6
Lip Sal
Cebador complementarioc 5´GCG CGT CGA CGT GTA ATC TTG ATG CCA TCA GG´3 SEQ ID NO: 7
Sonda de unión al
Rossmanith et al., 2006,
LipProbe
locus prfAc FAM-CAG GAT TAA AAG TTG ACC GCA-MGB SEQ ID NO: 5
pLucLm 4
Sonda de unión a la diana artificial HEX-TTC GAA ATG TCC GTT CGG TTG GC-BHQ1 Rossmanith et al., 2006, SEQ ID NO: 4
IAC
Diana artificial 5´-GAT ACA GAA ACA TCG GTT GGC GTA TTC GAA ATG TCC GTT CGG TTG GCG CTA TGA AGA GAT ACG CGG TGG AAC CTG GAA CCT GAT GGC ATC AAG ATT ACA C-3´ Rossmanith et al., 2006, SEQ ID NO: 1
NC 16
Confirmación de la inserción de pPL2 5´-GTC AAA ACA TAC GCT CTT ATC-3´ Lauer et al., 2002
PL 95
Confirmación de la inserción de pPL2 5´-ACA TAA TCA GTC CAA AGT AGA TGC-3´ Lauer et al., 2002
Sequ. 1 (NC 16)
Confirmación de la inserción de pPL2 Secuencia incluida en la tabla 1A Este trabajo
Sequ. 2 (PL 95)
Confirmación de la inserción de pPL2 Secuencia incluida en la tabla 1A Este trabajo
Especie / Plásmido
Cepa
d
L. monocytogenes
EGDe (1/2a) Cepa de tipo salvaje detectada mediante PCR en tiempo real para prfA IMML cepa No. 2964
L. monocytogenes
EGDe (1/2a) Cepa L. monocytogenes EGDe con deleción total Böckmann et al., 1996
Δdiana
ΔprfA del locus prfA
E. coli TOP10F´
-E. coli químicamente competente Invitrogen
Vector de inserción
Específico para L. Sitio de integración PSA. Integrantes de copia Lauer et al., 2002
basado en fago pPL2
monocytogenes única estables: –10-4/células donadoras.
a Específico para L. monocytogenes de tipo salvaje b Cebador que contiene el sitio de restricción BamHI. c Cebador que contiene el sitio de restricción SalI. d IMML: Instituto de Higiene de la Leche, Tecnología de la Leche y Ciencia de los Alimentos, Universidad de Medicina Veterinaria
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Claims (1)

  1. imagen1
    imagen2
ES11716841.9T 2010-06-02 2011-04-29 Bacteria genéticamente modificada de la especie Listeria monocytogenes Active ES2539970T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP10005731 2010-06-02
EP10005731 2010-06-02
PCT/EP2011/002147 WO2011150999A1 (en) 2010-06-02 2011-04-29 A genetically modified bacterium of the species listeria monocytogenes

Publications (1)

Publication Number Publication Date
ES2539970T3 true ES2539970T3 (es) 2015-07-07

Family

ID=44123196

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Application Number Title Priority Date Filing Date
ES11716841.9T Active ES2539970T3 (es) 2010-06-02 2011-04-29 Bacteria genéticamente modificada de la especie Listeria monocytogenes

Country Status (8)

Country Link
US (1) US8808683B2 (es)
EP (1) EP2576599B1 (es)
JP (1) JP5774095B2 (es)
CN (1) CN102906106B (es)
BR (1) BR112012030682B1 (es)
ES (1) ES2539970T3 (es)
MX (1) MX2012013890A (es)
WO (1) WO2011150999A1 (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102676506A (zh) * 2012-05-30 2012-09-19 曹际娟 单核细胞增生李斯特氏菌核酸标准样品、其建立方法及应用
WO2014093366A2 (en) * 2012-12-10 2014-06-19 Fred Hutchinson Cancer Research Center Megasphaera cerevisiae system process control (spc) primers, probes, and methods
WO2016153355A2 (en) * 2015-03-26 2016-09-29 Microbiome Limited Novel isolation and amplification process control
CN106978381A (zh) * 2017-03-23 2017-07-25 上海理工大学 prfA基因缺失单核细胞增生李斯特氏菌及制备方法
WO2020182924A1 (en) * 2019-03-14 2020-09-17 Merck Patent Gmbh A novel sampling method for long-term monitoring of microbes

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5801155A (en) 1995-04-03 1998-09-01 Epoch Pharmaceuticals, Inc. Covalently linked oligonucleotide minor grove binder conjugates
DE10012540B4 (de) * 2000-03-15 2004-09-23 Vermicon Ag Oligonukleotide und Verfahren zum spezifischen Nachweis von Mikroorganismen durch Polymerase-Kettenreaktion
US7019129B1 (en) 2000-05-09 2006-03-28 Biosearch Technologies, Inc. Dark quenchers for donor-acceptor energy transfer
EP2049677B1 (en) 2006-08-10 2011-07-20 Merck Patent GmbH Method for isolating cells
CN101177714A (zh) * 2007-11-15 2008-05-14 上海交通大学 添加扩增内标的单核细胞增生李斯特菌pcr检测方法
CN101368203B (zh) * 2008-09-16 2012-08-08 中国计量学院 单增李斯特菌环介导等温扩增技术快速检测用引物、试剂盒和检测方法

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Publication number Publication date
BR112012030682A2 (pt) 2020-08-25
BR112012030682B1 (pt) 2021-09-21
WO2011150999A1 (en) 2011-12-08
CN102906106B (zh) 2015-11-25
EP2576599B1 (en) 2015-03-18
JP2013532962A (ja) 2013-08-22
EP2576599A1 (en) 2013-04-10
JP5774095B2 (ja) 2015-09-02
MX2012013890A (es) 2012-12-17
US8808683B2 (en) 2014-08-19
CN102906106A (zh) 2013-01-30
US20130137100A1 (en) 2013-05-30

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