ES2538817T3 - Método para la inducción simultánea de CTL y células gamma delta T - Google Patents

Método para la inducción simultánea de CTL y células gamma delta T Download PDF

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Publication number
ES2538817T3
ES2538817T3 ES09773177.2T ES09773177T ES2538817T3 ES 2538817 T3 ES2538817 T3 ES 2538817T3 ES 09773177 T ES09773177 T ES 09773177T ES 2538817 T3 ES2538817 T3 ES 2538817T3
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cells
ctl
gamma delta
simultaneous induction
peripheral blood
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ES09773177.2T
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Mie Nieda
Mai Tomiyama
Masato Muto
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Medinet Co Ltd
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Medinet Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0636—T lymphocytes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/0005—Vertebrate antigens
    • A61K39/0011—Cancer antigens
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/46—Cellular immunotherapy
    • A61K39/461—Cellular immunotherapy characterised by the cell type used
    • A61K39/4611—T-cells, e.g. tumor infiltrating lymphocytes [TIL], lymphokine-activated killer cells [LAK] or regulatory T cells [Treg]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/46—Cellular immunotherapy
    • A61K39/464—Cellular immunotherapy characterised by the antigen targeted or presented
    • A61K39/4643—Vertebrate antigens
    • A61K39/4644—Cancer antigens
    • A61K39/464466—Adhesion molecules, e.g. NRCAM, EpCAM or cadherins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/04—Immunostimulants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0636—T lymphocytes
    • C12N5/0638—Cytotoxic T lymphocytes [CTL] or lymphokine activated killer cells [LAK]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K2035/124—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells the cells being hematopoietic, bone marrow derived or blood cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/515—Animal cells
    • A61K2039/5158—Antigen-pulsed cells, e.g. T-cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/57—Medicinal preparations containing antigens or antibodies characterised by the type of response, e.g. Th1, Th2
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/2302—Interleukin-2 (IL-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Hematology (AREA)
  • Epidemiology (AREA)
  • Mycology (AREA)
  • Oncology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Un método in vitro para producir una población celular mixta de CTL específicos de antígeno de enfermedad y células γ δT, en que el método comprende las etapas de: añadir un antígeno de enfermedad y un aminobifosfonato a una población de células mononucleares de sangre periférica y cultivar las células mononucleares de sangre periférica resultantes que comprenden dichos CTL específicos de antígeno de enfermedad y células γ δT.

Description

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Claims (1)

  1. imagen1
ES09773177.2T 2008-07-01 2009-06-30 Método para la inducción simultánea de CTL y células gamma delta T Active ES2538817T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2008172797 2008-07-01
JP2008172797 2008-07-01
PCT/JP2009/003039 WO2010001599A1 (ja) 2008-07-01 2009-06-30 ＣＴＬとγδＴ細胞の同時誘導方法

Publications (1)

Publication Number Publication Date
ES2538817T3 true ES2538817T3 (es) 2015-06-24

Family

ID=41465706

Family Applications (1)

Application Number Title Priority Date Filing Date
ES09773177.2T Active ES2538817T3 (es) 2008-07-01 2009-06-30 Método para la inducción simultánea de CTL y células gamma delta T

Country Status (9)

Country Link
US (1) US8962313B2 (es)
EP (1) EP2311470B1 (es)
JP (1) JP5524056B2 (es)
CN (1) CN102137925B (es)
DK (1) DK2311470T3 (es)
ES (1) ES2538817T3 (es)
HU (1) HUE027084T2 (es)
PT (1) PT2311470E (es)
WO (1) WO2010001599A1 (es)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2379088T3 (es) * 2004-07-08 2012-04-20 Medinet., Co. Ltd. Fármaco de células dendríticas que contienen células dendríticas, procedimiento terapéutico usando la célula dendrítica y procedimiento de cultivar células T gamma delta
DK2589267T3 (en) 2010-06-04 2014-03-24 Opulent Electronics Internat Pte Ltd Device and method for driving the LED
US20130295671A1 (en) * 2011-01-21 2013-11-07 Biotherapy Institute Of Japan Method for producing nk cell-enriched blood preparation
JP5825966B2 (ja) * 2011-10-11 2015-12-02 株式会社日本バイオセラピー研究所 Ｃｄ５６陽性ｔ細胞増強方法
KR20160068960A (ko) 2013-10-25 2016-06-15 보드 오브 리전츠, 더 유니버시티 오브 텍사스 시스템 면역요법을 위한 다클론성 감마 델타 t 세포
CN103768595B (zh) * 2014-01-27 2016-03-02 中国医学科学院医学生物学研究所 唑来膦酸佐剂及含唑来膦酸佐剂的疫苗
CN107106578B (zh) * 2014-08-12 2020-12-25 香港大学 治疗厄泼斯坦-巴尔病毒相关疾病的双膦酸盐化合物和γδT细胞-介导的疗法
WO2016055996A1 (en) * 2014-10-06 2016-04-14 Tel Hashomer Medical Research Infrastructure And Services Ltd. Expanding t cell populations using biphosphonates, anti cd 3 antibody and il-2
GB201421716D0 (en) * 2014-12-05 2015-01-21 King S College London Cell expansion procedure
DE102017127984B4 (de) 2017-11-27 2019-12-05 Immatics US, Inc. Verfahren für die Vermehrung und Aktivierung von γδ-T-Zellen
CN108949685B (zh) * 2018-08-02 2022-03-29 吉林大学第一医院 一种体外诱导扩增高杀伤活性γδT细胞的方法
AU2019349956A1 (en) 2018-09-27 2021-04-29 PhosphoGam Inc. Methods and compositions for the expansion and use of allogeneic gamma/delta-T cells
CN109517793B (zh) * 2018-11-30 2022-05-10 广州长峰生物技术有限公司 一种NK细胞和γδT细胞共培养的建立方法

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050130899A1 (en) 2001-12-10 2005-06-16 Kyogo Itoh Tumor antigens
US20050048646A1 (en) 2003-08-25 2005-03-03 Medinet Co., Ltd. Method for inducing cytotoxic T lymphocyte
ES2379088T3 (es) 2004-07-08 2012-04-20 Medinet., Co. Ltd. Fármaco de células dendríticas que contienen células dendríticas, procedimiento terapéutico usando la célula dendrítica y procedimiento de cultivar células T gamma delta
JPWO2006006720A1 (ja) 2004-07-13 2008-05-01 株式会社メディネット γδＴ細胞の培養方法、γδＴ細胞及び治療・予防剤
CN100591761C (zh) * 2004-08-19 2010-02-24 加的夫大学学院咨询有限公司 呈递抗原的人γδT细胞的制备和在免疫治疗中的用途
WO2007029689A1 (ja) 2005-09-08 2007-03-15 Medinet Co., Ltd. 抗原提示細胞の活性化処理方法

Also Published As

Publication number Publication date
WO2010001599A1 (ja) 2010-01-07
CN102137925B (zh) 2014-12-10
EP2311470A1 (en) 2011-04-20
EP2311470B1 (en) 2015-05-27
DK2311470T3 (en) 2015-08-17
US20120107292A1 (en) 2012-05-03
HUE027084T2 (en) 2016-08-29
EP2311470A4 (en) 2013-05-08
JPWO2010001599A1 (ja) 2011-12-15
US8962313B2 (en) 2015-02-24
CN102137925A (zh) 2011-07-27
PT2311470E (pt) 2015-07-09
JP5524056B2 (ja) 2014-06-18

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