CO6640286A2 - Proceso in vitro para la determinacion rapida en un paciente del estado de infeccion con mycrobacterium tuberculosis - Google Patents

Proceso in vitro para la determinacion rapida en un paciente del estado de infeccion con mycrobacterium tuberculosis

Info

Publication number
CO6640286A2
CO6640286A2 CO12162307A CO12162307A CO6640286A2 CO 6640286 A2 CO6640286 A2 CO 6640286A2 CO 12162307 A CO12162307 A CO 12162307A CO 12162307 A CO12162307 A CO 12162307A CO 6640286 A2 CO6640286 A2 CO 6640286A2
Authority
CO
Colombia
Prior art keywords
infection
tuberculosis
cells
rapid determination
status
Prior art date
Application number
CO12162307A
Other languages
English (en)
Inventor
Ferdinand Hermann Bahlmann
Danilo Fliser
Martina Sester
Urban Sester
Original Assignee
Red Flag Diagnostics Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Red Flag Diagnostics Gmbh filed Critical Red Flag Diagnostics Gmbh
Publication of CO6640286A2 publication Critical patent/CO6640286A2/es

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria
    • G01N33/5695—Mycobacteria
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6863—Cytokines, i.e. immune system proteins modifying a biological response such as cell growth proliferation or differentiation, e.g. TNF, CNF, GM-CSF, lymphotoxin, MIF or their receptors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6863—Cytokines, i.e. immune system proteins modifying a biological response such as cell growth proliferation or differentiation, e.g. TNF, CNF, GM-CSF, lymphotoxin, MIF or their receptors
    • G01N33/6866—Interferon
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6863—Cytokines, i.e. immune system proteins modifying a biological response such as cell growth proliferation or differentiation, e.g. TNF, CNF, GM-CSF, lymphotoxin, MIF or their receptors
    • G01N33/6869—Interleukin
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/52—Assays involving cytokines
    • G01N2333/54—Interleukins [IL]
    • G01N2333/55—IL-2
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/52—Assays involving cytokines
    • G01N2333/555—Interferons [IFN]
    • G01N2333/57—IFN-gamma
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/56—Staging of a disease; Further complications associated with the disease

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Un proceso in-vitro para la determinación rápida del estado de infección de una infección por Mycobacterium tuberculosis a partir de sangre completa en términos de una tuberculosis activa o latente, que comprende los pasos de:• estimular una célula T especifica del antígeno presente en una primera muestra de sangre completa con derivado de proteínas purificado (PPD) en la presencia de anticuerpos contra CD28 o CD28 y CD49d;• Procesar el PPD por células presentadoras de antígenos (APC) por incubación durante 1,5 h a 2,5 h, en especial durante 2h, a 35°C - 39°C, en especial a 37°C, opcionalmente con adición de CO;• Luego agregar un inhibidor de la secreción;• realizar un mezclado exhaustivo; y• incubar adicionalmente por un periodo da al manos 2,5 h a una temperatura de entre 35°C y 39°C, y• determinar el perfil de citoquinas a partir de le producción de INF-? intracelular y la producción de IL-2 intracelular de la célula T específica del antígeno; en donde• la presencia de una tuberculosis activa está indicada por un cambio en el perfil de citoquinas para células IFN-? simples positivas acompañado por una disminución de células T IFN-?/IL-2 positivas dobles.
CO12162307A 2010-03-19 2012-09-19 Proceso in vitro para la determinacion rapida en un paciente del estado de infeccion con mycrobacterium tuberculosis CO6640286A2 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US28270110P 2010-03-19 2010-03-19
EP10157086 2010-03-19

Publications (1)

Publication Number Publication Date
CO6640286A2 true CO6640286A2 (es) 2013-03-22

Family

ID=42457988

Family Applications (1)

Application Number Title Priority Date Filing Date
CO12162307A CO6640286A2 (es) 2010-03-19 2012-09-19 Proceso in vitro para la determinacion rapida en un paciente del estado de infeccion con mycrobacterium tuberculosis

Country Status (16)

Country Link
US (2) US20130122523A1 (es)
EP (1) EP2548019A1 (es)
JP (1) JP5925184B2 (es)
KR (1) KR20130029063A (es)
AU (1) AU2011228996B2 (es)
BR (1) BR112012023678A2 (es)
CA (1) CA2793151C (es)
CL (1) CL2012002542A1 (es)
CO (1) CO6640286A2 (es)
CU (1) CU20120141A7 (es)
MD (1) MD20120084A2 (es)
MX (1) MX2012010720A (es)
RU (1) RU2012144417A (es)
SG (1) SG184103A1 (es)
WO (1) WO2011113953A1 (es)
ZA (1) ZA201206950B (es)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2860056A1 (en) 2010-12-23 2012-06-28 Centre Hospitalier Universitaire Vaudois Methods for differentiating between disease states of mycobacterium tuberculosis infection
WO2012167307A1 (en) * 2011-06-06 2012-12-13 The University Of Melbourne Diagnosis of mycobacterial infection
US9678071B2 (en) 2012-01-12 2017-06-13 Mayo Foundation For Medical Education And Research Detecting latent tuberculosis infections
US10401360B2 (en) 2013-04-29 2019-09-03 Mayo Foundation For Medical Education And Research Flow cytometry assay methods
GB201315748D0 (en) 2013-09-04 2013-10-16 Imp Innovations Ltd Biological methods and materials for use therein
US9549914B2 (en) 2014-04-03 2017-01-24 The Johns Hopkins University Treatment of human cytomegalovirus by modulating Wnt
JP6306124B2 (ja) * 2016-11-01 2018-04-04 国立大学法人高知大学 結核検査用バイオマーカー
WO2018111536A1 (en) 2016-12-14 2018-06-21 Becton, Dickinson And Company Methods and compositions for obtaining a tuberculosis assessment in a subject
CN107831316A (zh) * 2017-10-31 2018-03-23 扬州大学 一种用于诊断牛结核病的流式细胞术检测试剂盒
CN109777852A (zh) * 2019-01-29 2019-05-21 浙江省人民医院 特异性抗原刺激cd4淋巴细胞表达cd69用于诊断结核杆菌感染的方法
KR102326294B1 (ko) * 2019-10-27 2021-11-16 바디텍메드(주) 결핵 진단 장치

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7514232B2 (en) 1996-12-06 2009-04-07 Becton, Dickinson And Company Method for detecting T cell response to specific antigens in whole blood
CZ20031866A3 (cs) * 2001-01-08 2004-03-17 Isis Innovation Limited Způsob pro stanovení účinnosti léčení mykobakteriální infekce
EP1833739A2 (de) * 2004-12-30 2007-09-19 Medmix Systems AG Mehrkomponenten-austragvorrichtung für flüssige und pulverförmige stoffe
BRPI0606165A2 (pt) * 2005-01-05 2009-06-02 Isis Innovation método de indução de uma resposta imunológica de célula t de memória contra pelo menos um antìgeno em um paciente humano, uso de uma composição imunogênica que compreende um vetor de vìrus de infecção virulenta não-replicante ou emparelhado de replicação que expressa o produto de tradução de um gene de ag85a micobacterial, uso de um antìgeno na manufatura de um medicamento para o tratamento de uma doença em um paciente mediante a indução de uma resposta imunológica de célula t de memória central e vacina vetorada
EP1767937A1 (en) * 2005-09-27 2007-03-28 Institut National De La Sante Et De La Recherche Medicale (Inserm) Method of diagnosis of tuberculosis related immune restoration syndrome (IRS)
GB0605474D0 (en) * 2006-03-17 2006-04-26 Isis Innovation Clinical correlates
JP5700934B2 (ja) 2007-03-16 2015-04-15 セルスティス リミテッド 細胞性免疫反応アッセイおよびそのためのキット

Also Published As

Publication number Publication date
ZA201206950B (en) 2013-05-29
MX2012010720A (es) 2013-01-29
BR112012023678A2 (pt) 2016-08-23
KR20130029063A (ko) 2013-03-21
CA2793151A1 (en) 2011-09-22
EP2548019A1 (en) 2013-01-23
CL2012002542A1 (es) 2013-02-08
CU20120141A7 (es) 2013-01-30
MD20120084A2 (ro) 2013-04-30
AU2011228996A1 (en) 2012-10-11
RU2012144417A (ru) 2014-04-27
AU2011228996B2 (en) 2015-01-29
JP5925184B2 (ja) 2016-05-25
JP2013522637A (ja) 2013-06-13
WO2011113953A1 (en) 2011-09-22
CA2793151C (en) 2018-05-22
US20130122523A1 (en) 2013-05-16
SG184103A1 (en) 2012-10-30
US20150099652A1 (en) 2015-04-09
US9476877B2 (en) 2016-10-25

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