ES2537423T3 - Ensayo para la metilación en el gen para la GST-Pi - Google Patents

Ensayo para la metilación en el gen para la GST-Pi Download PDF

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ES2537423T3
ES2537423T3 ES99914382.9T ES99914382T ES2537423T3 ES 2537423 T3 ES2537423 T3 ES 2537423T3 ES 99914382 T ES99914382 T ES 99914382T ES 2537423 T3 ES2537423 T3 ES 2537423T3
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gst
seq
dna
gene
exon
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Susan Joy Clark
Douglas S. Millar
Peter Laurence Molloy
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Commonwealth Scientific and Industrial Research Organization CSIRO
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C12N9/1088—Glutathione transferase (2.5.1.18)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/154—Methylation markers

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  • Chemical & Material Sciences (AREA)
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  • Health & Medical Sciences (AREA)
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  • Genetics & Genomics (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
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  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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Abstract

Un ensayo de diagnóstico o de pronóstico para el cáncer de próstata en un sujeto, dicho cáncer de próstata caracterizado por metilación anormal de la citosina en un sitio dentro de la región del gen para la glutatión S-transferasa humana (GST) Pi definido por uno cualquiera de los sitios CpG +1 a +33, en donde dicho ensayo comprende las etapas de: (i) aislar el ADN de dicho sujeto, y (ii) determinar la presencia de metilación anormal de la citosina en un sitio individual dentro de la región del gen para la GST-Pi humana definido por uno cualquiera de los sitios CpG +1 a +33.

Description

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E99914382
20-05-2015
Ed. (Birkliauser Verlag, Basel, Switzerland, 1993) pp. 404-424.
8.
C. Stirzaker, et al, Cancer Res 57, 2229-2237 (1997).
9.
K. D. Tremblay, K. L. Duran, M. S. Bartolomei, Mol Cell Biol Molecular and Cellular Biology 17, 4322-4329 (1997).
10.
R. Y.-H. Wang, Gehrke, C.W. and Ehriich, M., Nucl. Acids Res. 8, 4777-4790 (1980).
5 11. R. Shapiro, B. Braverman, J. B. Louis & R. E. Servis, /. Biol. Chem. 248, 4060-4064 (1973).
12.
M. Frommer, McDonald, L.E., Millar, D.S., Collis, CM., Watt, F., Grigg, G.W., Molloy, P.L. & Paul, C. L., Proc. Natl. Acad. Sci. USA. 89, 1827-1831 (1992).
13.
S. J. Clark, Harrison, J., Paul, C.L., & Frommer, M., Nucl. Acids Res. 22, 2990-2997 (1994).
14.
J. G. Herman, J. R. Graff, S. Myohanen, B. D. Nelkin &S. B. Baylin, Proc Natl Acad Sci USA 93, 9821-9826 (1996). 10 15. W. H. Lee, et al, Proc Natl Acad Sci USA 91, 11733-11737 (1994).
16.
S. J. Clark, M. Frommer, in Laboratory Methods for the detection of mutations and polymorphisms in DNA. G. Taylor, Ed. (CRC press, NewYork, 1997) pp. 151-162.
17.
C. L. Paul, S. J. Clark, BioTechniques 21, 126-133 (1996).
18.
W.H. Lee, W.B. Isaacs, G.S. Bova & W.G. Nelson, Cancer Epidemiol Biomarkers Prev. G, 443-450 (1997). 15 19. P.M. Holland, R.D. Abramson, R. Watson & D.H. Gelfland, Proc. Natl. Acad. Sci. USA 88, 7276-7280 (1991).
20.
F. Barany, Proc. Natl. Acad. Sci. USA 88, 189-193 (1991).
21.
R.D. Abramson and T.W. Myers, Curr. Opinion Biotechnol. 4, 41-47 (1993).
22.
U. Latza, G. Niedobitek, R. Schwarting, H. Nekarda and H.J. Stein, Clinical Pathology 43, 213-219 (1990).
23. H. Liu, P. Moy, S. Kim, Y. Xia, A. Rajasekaran, V. Navarro, B. Knudsen and N.H. Bander, Cancer Res. 57, 3629-3634 20 (1997).
Tabla 1
imagen15
16

Tabla 2.1 Cebadores para amplificación por PCR del gen para la GST-Pi modificado con bisulfito
PCR#
Objetivo Nombredelcebador Tipo decebador Cebador5' 3' Tamañoobjetivo(pb) HibridaciónºC PosiciónGenómica
-1
Hebra superiorde ADN endirección 5' GST-1GST-25GST-3GST-4 ExteriorInteriorInteriorExterior TTATGTAATAAATTTGTATATTTTGTATATG (SEQ ID NO: 23)TGTAGATTATTTAAGGTTAGGAGTT (SEQ ID NO: 24)AAACCTAAAAAATAAACAAACAACAAA (SEQ ID NO: 25)AAAAAACCTTTCCCTCTTTCCCAAATCCC (SEQ ID NO: 26) 646499499646 50/5050/5050/5050/50 381-411495-519967-993999-1027
1
Hebra superiorde ADN Exón 1 GST-9GST-11GST-12GST-10 ExteriorInteriorInteriorExterior TTTGTTGTTTGTTTATTTTTTAGGTTT (SEQ ID NO: 27)GGGATTTGGGAAAGAGGGAAAGGTTT (SEQ ID NO: 28)ACTAAAAACTCTAAACCCCATCCC (SEQ ID NO: 29)AACCTAATACTACC TTAACCCCAT (SEQ ID NO: 30) 346307307346 45/5045/5045/5045/50 967-993999-10251280-13031304-1329
2
Hebra inferiorde ADN Exón 1 GST-B1GST-B2GST-B3GST-B4 ExteriorInteriorInteriorExterior AATCCTCTTCCTACTATCTATTTACTCCCTAAA(SEQ ID NO: 31)AAAACCTAAAAAAAAAAAAAAAACTTCCC (SEQ ID NO: 32)TTGGTTTTATGTTGGGAGTTTTGAGTTTT (SEQ ID NO: 33)TTTTGTGGGGAGTTGGGGTTTGATGTTGT (SEQ ID NO: 34) 387314314387 50/5550/5550/5550/55 958-990999-10271285-13131317-1345
3
Hebra superiorde ADN Exón2/Exón 3 GST-13GST-14GST-15GST-16 ExteriorInteriorInteriorExterior GGTTTAGAGTTTTTAGTATGGGGTTAATT (SEQ ID NO: 35)TAGTATTAGGTTAGGGTTTT (SEQ ID NO: 36)AACTCTAACCCTAATCTACCAACAACATA (SEQ ID NO: 37)CA AAAAACTTTAAATAAACCCTCCTACCA (SEQ ID NO: 38) 691603603691 45/5045/5045/5045/50 1287-13151318-13371920-18921978-1950
4
Hebra superiorde ADN Exón 5 GST-30GST-31GST-32GST-33 ExteriorInteriorInteriorExterior GTTTTGTGGTTAGGTTGTTTTTTAGGTGTTAG (SEQ ID NO:39)GTTTTGAGTATTTGTTGTGTGGTAGTTTTT (SEQ ID NO: 40)TTAATATAAATAAAAAAAATATATTTACAA (SEQ ID NO: 41)CAACCCCCAATACCCAACCCTAATACAAATACTC (SEQ IDNO: 42) 340265265340 55/6040/4540/4555/60 2346-23762381-24162617-26462653-2686
5
Hebra superiorde ADN Exón 7 GST-26GST-27GST-28GST-29 ExteriorInteriorInteriorExterior GGTTTTAGTTTTTGGTTGTTTGGATG (SEQ ID NO: 43)TTTTTTTGTTTTTAGTATATGTGGGG (SEQ ID NO: 44)ATACTAAAAAAACTATTTTCTAATCCTCTA (SEQ ID NO: 45)CCAAACTAAAAACTCCAAAAAACCACTAA (SEQ ID NO: 46) 347287287347 50/5550/5550/5550/55 3845-38693874-38994161-41324192-4164
Las bases que surgen debido a la conversión de C en U por el tratamiento con bisulfito se muestran en negrita
17

Tabla 2.2 Cebadores para secuenciación directa de fragmentos amplificados por PCR del gen para la GST-Pi
PCR#
Objetivo Nombredelcebador Tipo decebador Cebador5' 3' Tamañoobjetivo(pb) HibridaciónºC PosiciónGenómica
1
Hebra superiorde ADN Exón 1 GST-11GST-12 M13Biotina TGTAAAACGACGGCCAGTGGGATTTGGGAAAGAGGGAA(SEQ ID NO: 47)BioACTAAAAACTCTAAACCCCATCCC 307307 45/5045/50 1003-10261288-1313
2
Hebra inferiorde ADN Exón 1 GST-B2GST-B2 M13Biotina TGTAAAACGACGGCCAGTTGTTGGGAGTTTTGAGTTTT(SEQ ID NO: 48)BioAAAACCTAAAAAAAAAAAAAAAACTTCCC ( 314314 50/5550/55 999-10271285-1313
3
Hebra superiorde ADN Exón2/3 GST-14GST-15 M13Biotina TGTAAAACGACGGCCAGTTAGTATTAGGTTA (SEQ ID NO:49)BioAACTCTAACCCTAATCTACCAACAACATA 603603 45/5045/50 1317-13371920-1892
4
Hebra superiorde ADN Exón4/5 GST-31GST-32 M13Biotina TGTAAAACGACGGCCAGTGTTTTGAGTATTTGTTGTG (SEQID NO: 50)BioTTAATATAAATAAAAAAAATATATTTTACAA 265265 55/6055/60 2381-24102617-2646
5
Hebra superiorde ADN Exón 7 GST-27GST-28 M13Biotina TGTAAAACGACGGCCAGTGTTTTTAGTATATGTGG (SEQ IDNO: 51)BioATACTAAAAAAACTATTTTCTAATCCTCTA 287287 50/5550/55 3874-41324161-4164
Las extensiones sobre cebadores "M13" para la hibridación del cebador de secuenciación están subrayadas.
18

Claims (1)

  1. imagen1
    imagen2
ES99914382.9T 1998-04-23 1999-04-23 Ensayo para la metilación en el gen para la GST-Pi Expired - Lifetime ES2537423T3 (es)

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AUPP3129A AUPP312998A0 (en) 1998-04-23 1998-04-23 Diagnostic assay
AUPP312998 1998-04-23
PCT/AU1999/000306 WO1999055905A1 (en) 1998-04-23 1999-04-23 ASSAY FOR METHYLATION IN THE GST-Pi GENE

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US6818404B2 (en) * 1997-10-23 2004-11-16 Exact Sciences Corporation Methods for detecting hypermethylated nucleic acid in heterogeneous biological samples
AUPP312998A0 (en) * 1998-04-23 1998-05-14 Commonwealth Scientific And Industrial Research Organisation Diagnostic assay
US6331393B1 (en) 1999-05-14 2001-12-18 University Of Southern California Process for high-throughput DNA methylation analysis
DE19959691A1 (de) * 1999-12-06 2001-08-16 Epigenomics Ag Verfahren zur parallelen Detektions des Methylierungszustandes von genomischer DNA
DE10010282B4 (de) 2000-02-25 2006-11-16 Epigenomics Ag Verfahren zur Detektion von Cytosin-Methylierung in DNA Proben
DE10010280B4 (de) 2000-02-25 2006-08-10 Epigenomics Ag Verfahren zur Detektion von Cytosin-Methylierung in DNA Proben
GB0025913D0 (en) 2000-10-23 2000-12-06 Guldberg Per Materials and methods relating to nucleic acid amplification and profiling
AU1070002A (en) * 2000-10-23 2002-05-06 Cancer Res Ventures Ltd Materials and methods relating to nucleic acid amplification and profiling
DE10112515B4 (de) * 2001-03-09 2004-02-12 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierungsmustern mit hoher Sensitivität
DE10128508A1 (de) * 2001-06-14 2003-02-06 Epigenomics Ag Verfahren und Nukleinsäuren für die Differenzierung von Prostata-Tumoren
DE10130800B4 (de) * 2001-06-22 2005-06-23 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierung mit hoher Sensitivität
US7252935B2 (en) * 2001-11-16 2007-08-07 The John Hopkins University School Of Medicine Method of detection of prostate cancer
AU2003212087B2 (en) * 2002-02-26 2008-06-12 Commonwealth Scientific And Industrial Research Organisation Headloop DNA amplification
AUPS076802A0 (en) * 2002-02-26 2002-03-21 Commonwealth Scientific And Industrial Research Organisation Selective nucleic acid amplification
DE10230692A1 (de) * 2002-07-08 2004-02-12 Epigenomics Ag Verfahren und Nukleinsäuren für die Analyse von Methylierungsmustern innerhalb des DD3-Gens
US7238518B2 (en) * 2002-10-04 2007-07-03 Nisshinbo Industries, Inc. Oligonucleotide-immobilized substrate for detecting methylation
US20040146868A1 (en) * 2003-01-24 2004-07-29 Epigenomics Ag Methods and nucleic acids for the analysis of CpG dinucleotide methylation status associated with the development of peripheral zone prostate cancer
US20040203004A1 (en) * 2003-04-10 2004-10-14 Bernard Hans Ulrich Diagnostic apparatus and method
WO2004113567A2 (en) * 2003-06-24 2004-12-29 Epigenomics Ag Improved heavymethyl assay for the methylation analysis of the gstpi gene
EP1649052A1 (en) * 2003-08-01 2006-04-26 The University Of Western Australia Methods and kits for predicting the likelihood of successful treatment of cancer
EP3269826B1 (en) 2003-12-01 2020-03-11 Epigenomics AG Methods and nucleic acids for the analysis of gene expression associated with the development of prostate cell proliferative disorders
EP1730160A4 (en) * 2004-03-17 2008-04-09 Univ Johns Hopkins COMPOSITIONS FOR THE DIAGNOSIS OF NEOPLASIA AND METHOD OF USE
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US20070059753A1 (en) * 2005-09-15 2007-03-15 Tatiana Vener Detecting gene methylation
EP3078751B1 (en) * 2007-11-23 2018-08-01 Epigenomics AG Methods and uses of nucleic acids for the analysis of methylation and expression of genes, in particular of nfatc3, associated with prostate cell proliferative disorders
CN102016067A (zh) * 2008-01-22 2011-04-13 维里德克斯有限责任公司 前列腺癌中gstp1高甲基化的检测
WO2009140666A2 (en) 2008-05-15 2009-11-19 Ribomed Biotechnologies, Inc. METHODS AND REAGENTS FOR DETECTING CpG METHYLATION WITH A METHYL CpG BINDING PROTEIN (MBP)
CA2755668A1 (en) 2009-03-15 2010-09-23 Ribomed Biotechnologies, Inc. Abscription based molecular detection
US20140274757A1 (en) 2013-03-14 2014-09-18 Marie K. Kirby Differential Methylation Level of CpG Loci That Are Determinative of a Biochemical Reoccurrence of Prostate Cancer

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US555277A (en) * 1896-02-25 Buckle
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KR102110886B1 (ko) 2014-04-24 2020-05-14 삼성전자주식회사 기판 탑재형 방사체를 구비한 휴대용 전자 장치

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CA2326494C (en) 2005-11-29
EP2357256B1 (en) 2016-03-16
EP2357256A2 (en) 2011-08-17
HK1161309A1 (zh) 2012-08-24
DK2357256T3 (en) 2016-04-18
US20090215056A1 (en) 2009-08-27
EP1071815A1 (en) 2001-01-31
US8088577B2 (en) 2012-01-03
NZ507987A (en) 2004-02-27
ZA200006803B (en) 2001-05-10
JP2002512810A (ja) 2002-05-08
CA2326494A1 (en) 1999-11-04
EP1071815A4 (en) 2005-01-26
US20120231452A1 (en) 2012-09-13
HK1161308A1 (zh) 2012-08-24
WO1999055905A1 (en) 1999-11-04
AU3324199A (en) 1999-11-16
ES2568900T3 (es) 2016-05-05
DK1071815T3 (en) 2015-06-08
US20060269937A1 (en) 2006-11-30
EP2357256A3 (en) 2011-09-28
EP2357255A2 (en) 2011-08-17
EP2357255A3 (en) 2011-09-28
EP1071815B1 (en) 2015-03-25
ES2568770T3 (es) 2016-05-04
AUPP312998A0 (en) 1998-05-14
JP3522690B2 (ja) 2004-04-26
US7049062B1 (en) 2006-05-23
DK2357255T3 (en) 2016-04-18
AU768791B2 (en) 2004-01-08
EP2357255B1 (en) 2016-03-16

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