ES2537094T3 - Enterotoxina termolábil de E. coli mutada - Google Patents

Enterotoxina termolábil de E. coli mutada Download PDF

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Publication number
ES2537094T3
ES2537094T3 ES07868328.1T ES07868328T ES2537094T3 ES 2537094 T3 ES2537094 T3 ES 2537094T3 ES 07868328 T ES07868328 T ES 07868328T ES 2537094 T3 ES2537094 T3 ES 2537094T3
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coli
thermolabile enterotoxin
seq
subunit
enterotoxin
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ES07868328.1T
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Yu-Shen Hsu
Young-Sun Lin
Ta-Tung Yuan
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Development Center for Biotechnology
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Development Center for Biotechnology
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Priority claimed from US11/779,419 external-priority patent/US8110197B2/en
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/02—Bacterial antigens
    • A61K39/025—Enterobacteriales, e.g. Enterobacter
    • A61K39/0258—Escherichia
    • A—HUMAN NECESSITIES
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    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/02—Bacterial antigens
    • A—HUMAN NECESSITIES
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    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A61K39/145—Orthomyxoviridae, e.g. influenza virus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/14—Antivirals for RNA viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/14—Antivirals for RNA viruses
    • A61P31/16—Antivirals for RNA viruses for influenza or rhinoviruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/14—Antivirals for RNA viruses
    • A61P31/18—Antivirals for RNA viruses for HIV
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/20—Antivirals for DNA viruses
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    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C07K14/425—Zeins
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/525—Virus
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    • A61K2039/54—Medicinal preparations containing antigens or antibodies characterised by the route of administration
    • A61K2039/541—Mucosal route
    • A61K2039/543—Mucosal route intranasal
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    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/555—Medicinal preparations containing antigens or antibodies characterised by a specific combination antigen/adjuvant
    • A61K2039/55511—Organic adjuvants
    • A61K2039/55544—Bacterial toxins
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    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16111—Influenzavirus A, i.e. influenza A virus
    • C12N2760/16134—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
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    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
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  • Health & Medical Sciences (AREA)
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  • Organic Chemistry (AREA)
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  • General Engineering & Computer Science (AREA)
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  • Proteomics, Peptides & Aminoacids (AREA)
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  • Pulmonology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • AIDS & HIV (AREA)
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  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

Un polipéptido aislado que comprende una subunidad A de enterotoxina termolábil de E. coli, teniendo la subunidad A de enterotoxina termolábil de E. coli una secuencia de aminoácidos seleccionada de un grupo que consiste en los SEQ ID NO: 2, 4, 8, y 10, en donde una enterotoxina termolábil de E. coli que contiene la subunidad A que tiene una secuencia de aminoácidos seleccionada a partir de el grupo que consiste en los SEQ ID NO: 2, 4, 8 y 10 tiene una toxicidad reducida en comparación con una enterotoxina termolábil de E. coli de tipo salvaje que contiene el SEQ ID NO: 5.

Description

imagen1
imagen2
imagen3
imagen4
imagen5
E07868328
18-05-2015
imagen6
Secuencia de aminoácidos (SEQ ID NO: 8) de LTA (S61H)
imagen7
Secuencia de nucleótidos (SEQ ID NO: 9) de LTA(S61Y)
imagen8
Secuencia de aminoácidos (SEQ ID NO: 10) de LTA(S61Y)
imagen9
Para la comparación, también se construyó otro mutante de LT, LTp (S63K), derivado de EWD299 (Dallas et al., 10 1979, J. Bacteriol. 139, 850-859) mediante la sustitución de serina de la posición de aminoácido 63 de la subunidad A por lisina.
Ejemplo 2: Preparación de LT de tipo salvaje y LT que contiene LTA mutada
7
imagen10
imagen11
imagen12

Claims (1)

  1. imagen1
ES07868328.1T 2007-07-18 2007-08-13 Enterotoxina termolábil de E. coli mutada Active ES2537094T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US779419 1991-10-17
US11/779,419 US8110197B2 (en) 2006-10-27 2007-07-18 Mutated E. coli heat-labile enterotoxin
PCT/US2007/075801 WO2009011707A1 (en) 2007-07-18 2007-08-13 Mutated e. coli heat-labile enterotoxin

Publications (1)

Publication Number Publication Date
ES2537094T3 true ES2537094T3 (es) 2015-06-02

Family

ID=40262240

Family Applications (1)

Application Number Title Priority Date Filing Date
ES07868328.1T Active ES2537094T3 (es) 2007-07-18 2007-08-13 Enterotoxina termolábil de E. coli mutada

Country Status (8)

Country Link
EP (1) EP2170377B1 (es)
JP (2) JP5576792B2 (es)
KR (1) KR101699521B1 (es)
CN (1) CN101687026B (es)
BR (1) BRPI0721746B1 (es)
ES (1) ES2537094T3 (es)
RU (1) RU2441879C2 (es)
WO (1) WO2009011707A1 (es)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8241608B2 (en) * 2010-03-23 2012-08-14 Development Center For Biotechnology Treating allergy with detoxified E. coli heat-labile enterotoxin
CN102858370A (zh) * 2010-05-03 2013-01-02 Dcb-美国有限责任公司 用作疫苗的多糖与去毒大肠杆菌(e.coli)不耐热肠毒素(lt)的偶联
CN113616783A (zh) * 2020-05-08 2021-11-09 昱厚生技股份有限公司 使用免疫调节剂和包含所述免疫调节剂的疫苗组合物预防或治疗冠状病毒感染的方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6019982A (en) * 1994-08-26 2000-02-01 The Administrators Of The Tulane Educational Fund Mutant enterotoxin effective as a non-toxic oral adjuvant
CA2396871A1 (en) * 2000-01-20 2001-12-20 Ottawa Health Research Institute Immunostimulatory nucleic acids for inducing a th2 immune response
EP1272210A1 (en) * 2000-03-17 2003-01-08 The Uab Research Foundation Chimeric nontoxic mutants of enterotoxins as mucosal adjuvants for cell-mediated or humoral immunity
US20040171565A1 (en) * 2003-02-14 2004-09-02 David Hone DNA vaccines that expresses mutant ADP-ribosyltransferase toxins which display reduced, or are devoid of, ADP-ribosyltransferase activity
US8110197B2 (en) * 2006-10-27 2012-02-07 Development Center For Biotechnology Mutated E. coli heat-labile enterotoxin

Also Published As

Publication number Publication date
RU2010105693A (ru) 2011-09-20
EP2170377A4 (en) 2010-12-29
CN101687026B (zh) 2012-07-18
KR20100054765A (ko) 2010-05-25
BRPI0721746A2 (pt) 2018-10-23
WO2009011707A1 (en) 2009-01-22
JP5576792B2 (ja) 2014-08-20
BRPI0721746B1 (pt) 2021-08-31
EP2170377B1 (en) 2015-03-04
RU2441879C2 (ru) 2012-02-10
EP2170377A1 (en) 2010-04-07
JP2014148513A (ja) 2014-08-21
KR101699521B1 (ko) 2017-01-24
JP2010533489A (ja) 2010-10-28
JP5913406B2 (ja) 2016-04-27
CN101687026A (zh) 2010-03-31

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ES2537094T3 (es) Enterotoxina termolábil de E. coli mutada